Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,797,083	C→T	V288M (GTG→ATG)	pheT ←	phenylalanine‑‑tRNA ligase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,797,083	0	C	T	100.0%	211.4 / NA	62	V288M (GTG→ATG)	pheT	phenylalanine‑‑tRNA ligase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/29);  total (33/29)

ACCGCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCC  >  NC_000913/1796790‑1797375                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                     accGCAGATGTCGATCAGCAGACGTGTCGCACGCTCTATCGCTGTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACAGTTAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCGTTCCAGCAGCACGTTTTGTTTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCTCCCATCGCCAGCGCCTTGTTGTGGTCGTCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGt                                                                                                                                                                                                                                                                                                <  2:279263/300‑1 (MQ=255) accGCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCATGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGTTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGt                                                                                                                                                                                                                                                                                                <  2:12636/300‑1 (MQ=255) accGCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTTAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCg                                                                                                                                                                                                                                                                                                 <  2:559248/299‑1 (MQ=255) accGCAGATGTAGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGTTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCg                                                                                                                                                                                                                                                                                                 <  1:211914/299‑1 (MQ=255) accGCAGATGTAGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCg                                                                                                                                                                                                                                                                                                 <  1:211929/299‑1 (MQ=255)             gATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCttt                                                                                                                                                                                                                                                                                    >  1:169028/1‑300 (MQ=255)             gATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCACTCttt                                                                                                                                                                                                                                                                                    >  1:169041/1‑300 (MQ=255)                 agcagACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTTATACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGc                                                                                                                                                                                                                                                                                 <  2:253493/299‑1 (MQ=255)                    agACGGTTCGAACTTTCCATCGCTTTGTGCGTCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGGATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAGAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCACCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACTAGAGTGTCAGTATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATc                                                                                                                                                                                                                                                                             <  2:610660/300‑1 (MQ=255)                    agACGGGTCGCACGTTCCATCGCTTTGTGCGGCAGTGCCGGATCAACGCCACGCTCATAACGGTTAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATc                                                                                                                                                                                                                                                                             <  2:610669/300‑1 (MQ=255)                         ggTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGcac                                                                                                                                                                                                                                                                        >  1:325152/1‑300 (MQ=255)                                 gTTCCATCGCTTTGTGCTGCATTGCCGGATCAACTCCACGCTCATAACGTTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATgc                                                                                                                                                                                                                                                                <  1:259817/300‑1 (MQ=255)                                 gTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATgc                                                                                                                                                                                                                                                                <  1:259808/300‑1 (MQ=255)                                   tCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTTAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCNCCACCGAAGATGCCGCCCATCGCCAGCGCCTTNTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATgccg                                                                                                                                                                                                                                                              <  2:515134/300‑1 (MQ=255)                                              gtgCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGAACGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCCCTTTCGCCATCCGCACCCCAATGCCGCCTTCAATGc                                                                                                                                                                                                                                                    >  2:400248/1‑299 (MQ=255)                                                            gATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGaaa                                                                                                                                                                                                                                      >  1:404204/1‑299 (MQ=255)                                                                 aCGCCACGCTCATAACGGTTAGACGCATCGGTATGCATGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCACATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTTTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCTCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGGAAGCGTGc                                                                                                                                                                                                                                <  2:12317/300‑1 (MQ=255)                                                                 aCGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCATTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGCTTCGCCCTCTTTCGCCACCCGCACCACAATGCCGCCTTCAATGCGATCTTTACCGAAAGCGTGc                                                                                                                                                                                                                                >  2:313866/1‑300 (MQ=255)                                                                  cGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCa                                                                                                                                                                                                                               >  1:656233/1‑300 (MQ=255)                                                                   gCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCAt                                                                                                                                                                                                                              >  1:46890/1‑300 (MQ=255)                                                                        gCTCATAACGTTTAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGc                                                                                                                                                                                                                          <  1:179230/299‑1 (MQ=255)                                                                        gCTCATAACGGTGAGACGCATTGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGc                                                                                                                                                                                                                          <  1:179118/299‑1 (MQ=255)                                                                           cATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGc                                                                                                                                                                                                                      >  1:99684/1‑300 (MQ=255)                                                                                           aTCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCGAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCTAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGcac                                                                                                                                                                                                      <  2:389838/300‑1 (MQ=255)                                                                                            tCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATTACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGcaca                                                                                                                                                                                                     >  1:523929/1‑300 (MQ=255)                                                                                              ggTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGATCCGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCACAGTTCGAGCAGCACAt                                                                                                                                                                                                    >  1:558762/1‑299 (MQ=255)                                                                                              ggTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGNCATCCGCACCACAATGCAGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATa                                                                                                                                                                                                   >  1:155101/1‑300 (MQ=255)                                                                                                      ggCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGa                                                                                                                                                                                           >  1:168302/1‑300 (MQ=255)                                                                                                                           cGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATa                                                                                                                                                                       >  1:647278/1‑299 (MQ=255)                                                                                                                            ggTGATAGACAGCGGGCGAAAGAACGCGCATTACAACAGCACTTTTTGTGTTCCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGTTCTTCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGGACCGTCGAGCAGCATCAGCGGTTCGCCCTCTTGCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGTCCAGTTCGAGCAGCACATAGTTGTTGACGTCAACAACTGCATCGATAg                                                                                                                                                                      <  2:316192/299‑1 (MQ=255)                                                                                                                             gTGATAGACAGCGGGCTAAAGACCGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGCTCGCCAACGAAGATGCCGCCCGTCGCAAGCGCCTTGTTGTGGTTGGCGATGACCAGAGTGTCAGCAGTCAGCTTCGCTTCAGTACCGTCGCGCAGCATCAGCGTTTCGCACTCTTTCGCCATCTGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGTTGGCCCAGTTCGAGCAACACATAGTTGGTGCCGTCAACAACTGAATCGATAGaa                                                                                                                                                                    >  2:48798/1‑300 (MQ=255)                                                                                                                              tGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTTTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAAc                                                                                                                                                                   <  2:28874/300‑1 (MQ=255)                                                                                                                                         gggCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCaa                                                                                                                                                        >  1:62670/1‑300 (MQ=255)                                                                                                                                          ggCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCacc                                                                                                                                                       >  2:669999/1‑298 (MQ=255)                                                                                                                                          ggCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAac                                                                                                                                                       >  2:670242/1‑300 (MQ=255)                                                                                                                                          ggCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCAAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCACGCaa                                                                                                                                                        >  2:397609/1‑299 (MQ=255)                                                                                                                                                       gcATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATGGAACGGATCCCGCAACGACGCAGTTtttc                                                                                                                                          <  1:420466/300‑1 (MQ=255)                                                                                                                                                         agtCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGTNGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTtttctt                                                                                                                                        <  2:534527/298‑1 (MQ=255)                                                                                                                                                          ttCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTtttcttt                                                                                                                                       >  1:70395/1‑300 (MQ=255)                                                                                                                                                                 agcaCGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCcac                                                                                                                                <  1:358379/300‑1 (MQ=255)                                                                                                                                                                 agcaCGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCcac                                                                                                                                <  2:46890/300‑1 (MQ=255)                                                                                                                                                                                         aCGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTa                                                                                                        >  1:640738/1‑300 (MQ=255)                                                                                                                                                                                              agagTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGcc                                                                                                   <  1:427130/300‑1 (MQ=255)                                                                                                                                                                                                 gtgtTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCttt                                                                                                >  1:391023/1‑300 (MQ=255)                                                                                                                                                                                                       gCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCNGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACcac                                                                                          <  1:487520/300‑1 (MQ=255)                                                                                                                                                                                                          aCCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAANTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCacac                                                                                        >  2:528681/1‑299 (MQ=255)                                                                                                                                                                                                                  tGCCTCCCCTCGCCAGCTCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGAt                                                                               <  2:249766/300‑1 (MQ=255)                                                                                                                                                                                                                                      ttgttgTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCGGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGgc                                                           >  1:637210/1‑300 (MQ=255)                                                                                                                                                                                                                                      ttgttgTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGgc                                                           >  1:637202/1‑300 (MQ=255)                                                                                                                                                                                                                                        gttgtGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGgcgc                                                         >  2:670691/1‑300 (MQ=255)                                                                                                                                                                                                                                             ggTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGACCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTACTGCCTTTTACCACACGGCCAAGGTAACGAGGGCAGGCTTCCGGCGCTTCGa                                                    >  1:126764/1‑300 (MQ=255)                                                                                                                                                                                                                                                      tGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCg                                           <  1:528681/300‑1 (MQ=255)                                                                                                                                                                                                                                                        aCCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCGGTTTTTCTTTCATCCACAGCGGAGTTGGCGATTTAACGTTAATGCCTTTTACCACACGGACAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGc                                         >  2:640689/1‑300 (MQ=255)                                                                                                                                                                                                                                                           agagTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAg                                       <  1:48798/299‑1 (MQ=255)                                                                                                                                                                                                                                                           agagTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTCTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAg                                       <  1:46429/299‑1 (MQ=255)                                                                                                                                                                                                                                                            gagTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGc                                      >  1:609038/1‑299 (MQ=255)                                                                                                                                                                                                                                                                 tCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGTATCAGCATTTCGCCCTCTTTCACCATCCGCACCCCAATGCCGACTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCCAAGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCACGATAACGCAGGCAGGCTTCCGGCGCTTCGACTGTaa                                              >  2:383413/1‑286 (MQ=255)                                                                                                                                                                                                                                                                   aGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTgtcgt                               <  1:184599/299‑1 (MQ=255)                                                                                                                                                                                                                                                                              tcgcttcAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCAc                   <  1:363643/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 cttcAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCACCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTCAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCaa                >  1:664303/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    cAGTACCGTCGAGCAGCATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAAcc              >  1:655937/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                               agcagcATCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTcc  >  2:132635/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                     ACCGCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGCATTCCAGCAGCACGTTTTGTGTTTCGTCATTCACGCCAGAGTGTTCGCCACCGAAGATGCCGCCCATCGCCAGCGCCTTGTTGTGGTCGGCGATGACCAGAGTGTCAGCATTCAGCTTCGCTTCAGTACCGTCGAGCAGCACCAGCGTTTCGCCCTCTTTCGCCATCCGCACCACAATGCCGCCTTCAATGCGATCTTTATCGAAAGCGTGCATCGGCTGGCCCAGTTCGAGCAGCACATAGTTGGTGACGTCAACAACTGCATCGATAGAACGGATCCCGCAACGACGCAGTTTTTCTTTCATCCACAGCGGAGTTGGCGCTTTAACGTTAATGCCTTTTACCACACGGCCAAGATAACGCGGGCAGGCTTCCGGCGCTTCGACTGTAATCGGCAGCGTGTCGTCGATGGTCGCACCAACCGGAACGATTTCC  >  NC_000913/1796790‑1797375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑