Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,322,288	A→G	F45F (TTT→TTC)	phnF ←	putative transcriptional regulator PhnF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,322,288	0	A	G	97.7%	146.3 / ‑5.5	45	F45F (TTT→TTC)	phnF	putative transcriptional regulator PhnF
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (28/15);  total (29/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

GACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCA‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCT  >  NC_000913/4322014‑4322581                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                       gacgCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCAAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCCATACCGAAAGTAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGTGCTGTACCCAGCCTTTTTCAACCAGTTGGTCGATGGCGTGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGACAGTTGCTGCTc                                                                                                                                                                                                                                                                                >  2:42907/1‑298 (MQ=255)       gggTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGTTCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGaa                                                                                                                                                                                                                                                                        >  1:431736/1‑300 (MQ=255)       gggTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCAAGTGCGTCAGCGACGTGGACGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAAATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑CAGCGCGCTTCCAGTTGCTTCTCGGCGGGa                                                                                                                                                                                                                                                                         >  1:602861/1‑299 (MQ=255)       gggTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCACAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGaa                                                                                                                                                                                                                                                                        >  1:602854/1‑300 (MQ=255)         gTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGa                                                                                                                                                                                                                                                                      >  1:186640/1‑300 (MQ=255)                                       gTAATCCCCAGTGCGTAAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGGTAGTCGCCGCAGCGGTAGTGTTGACGAAGc                                                                                                                                                                                                                                        >  2:565762/1‑300 (MQ=255)                                                                      aCGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGAt                                                                                                                                                                                                         <  1:606675/300‑1 (MQ=255)                                                                      aCGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGAGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGAt                                                                                                                                                                                                         <  1:26259/300‑1 (MQ=255)                                                                             gcgcAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCgtgt                                                                                                                                                                                                  >  1:265568/1‑300 (MQ=255)                                                                             gcgcAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCCCTCTCTTGATAGCgtgt                                                                                                                                                                                                  >  1:265974/1‑300 (MQ=255)                                                                                  aTACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTTGCTAAAACGCGCCGGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCTGCGCAGGGGGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCTGGAAGAGAGTCGCCGCAGCGGTAGTGTTGACGAAGCCCCTGCTCAAGTTTTGCTGCTATCTCTTGATAGCGTGTTGGGt                                                                                                                                                                                             <  2:602861/300‑1 (MQ=255)                                                                                  aTACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACTGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGt                                                                                                                                                                                             <  2:163952/300‑1 (MQ=255)                                                                                  aTACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGt                                                                                                                                                                                             <  1:42907/300‑1 (MQ=255)                                                                                  aTACCGAAAGCAGTTTGTCGCTGGGGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGt                                                                                                                                                                                             <  2:602854/300‑1 (MQ=255)                                                                                       gAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTg                                                                                                                                                                                        <  1:363861/300‑1 (MQ=255)                                                                                                       tgttggGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGTTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGTCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACa                                                                                                                                                                        <  2:387973/297‑1 (MQ=255)                                                                                                       tggtggGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACa                                                                                                                                                                        <  1:335549/300‑1 (MQ=255)                                                                                                       tggtggGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAAGCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATATTCGCCGCAGCGGTAGTGTTGACTAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACa                                                                                                                                                                        <  1:333529/300‑1 (MQ=255)                                                                                                               tGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCACTGACGGCGCTGTACCCAGCCTTTTTCCACAAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCGGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTATTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATa                                                                                                                                                                >  2:345208/1‑300 (MQ=255)                                                                                                               tGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCAACGCACTGGCGGCGCCGTCCCCCACCTTCTTACACAAGTGGATAAACAGCGCGGCGCATGGCGCGGCGGTTCACCTCG‑AAGGGCGTTGTTAGTTGCTGCTCGGCGGGAGGGTAGGCGACGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTCGCTGGTCGGATGTGTAGAAAAGCGCATa                                                                                                                                                                >  2:344649/1‑300 (MQ=255)                                                                                                                 gCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGa                                                                                                                                                              <  1:546597/300‑1 (MQ=255)                                                                                                                 gCTGCCCTGATCCAGCAGATTCCTGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCTGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGa                                                                                                                                                              <  2:83176/300‑1 (MQ=255)                                                                                                                     cccTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATc                                                                                                                                                          >  1:262084/1‑300 (MQ=255)                                                                                                                         gATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCAACGCACTGACGGAGGTGTAGAGGGCATTTTTACAACAGTGTGTCGATGGTGAGGCGCCGGGTGTGGCGGTTCAC‑TCATATGCGCGTTGATCGTCGATGCTTGGTGGGAAGCTGGTGGCCGCAGTGGCAGTGTTGAAGCCGCTGCTGTTCAAGTTTTGTGGATAGCTATTGATAGAGTGGTGGGGAGATGGTCGGATGTGTAGGTGAGTGCGTAGAGATCAAt                                                                                                                                                       >  2:270805/1‑299 (MQ=255)                                                                                                                          aTCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGc                                                                                                                                                     <  1:645621/300‑1 (MQ=255)                                                                                                                               gcagATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGc                                                                                                                                                >  1:329572/1‑300 (MQ=255)                                                                                                                                     tCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTagc                                                                                                                                           <  1:6460/299‑3 (MQ=255)                                                                                                                                                         gCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTAGCTTATCAGATAAAGTGCTTACGCAAcc                                                                                                                      >  1:660659/1‑300 (MQ=255)                                                                                                                                                         gCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCAACAGTTGGTCGATGGCGCGGCGCAGGGTGTGGAGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGAAGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCaaac                                                                                                                      >  1:103805/1‑298 (MQ=255)                                                                                                                                                                                         caccagcacGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTNCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGt                                                                                       >  1:141940/1‑299 (MQ=255)                                                                                                                                                                                                                   gcTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAggg                                                                <  2:186640/296‑1 (MQ=255)                                                                                                                                                                                                                      gTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGt                                                         >  2:119513/1‑300 (MQ=255)                                                                                                                                                                                                                                       accaGTTGGTCGATGGCGCGGCGCAGGGTTTGGCGATTCACCTCT‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGATATAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGaa                                          <  2:329572/298‑1 (MQ=255)                                                                                                                                                                                                                                            ttGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGa                                   >  2:402357/1‑300 (MQ=255)                                                                                                                                                                                                                                            ttGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCATGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGa                                   >  2:338934/1‑300 (MQ=255)                                                                                                                                                                                                                                                           gcgcAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTccccca                      >  2:611696/1‑295 (MQ=255)                                                                                                                                                                                                                                                           gcgcAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCcaca                      >  1:518659/1‑298 (MQ=255)                                                                                                                                                                                                                                                           gcgcAGGGTGTGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAAACGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCcaca                      >  1:640241/1‑298 (MQ=255)                                                                                                                                                                                                                                                                  gtgtGGCGATTCACCTCG‑AAGCGTGCTGCCAGTTGCTGCTCGACGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCCATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGCTGTGTAGACAAGTGCATAGATATCAATGCCTAGCTTATCAGATACAGTGTTTAATAAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAGGCACAATCCGGGCGCCGGTTTGTTGGAACTTGAACCCGCGAATCGCTTCACACAGGGTggcga             >  2:200129/1‑296 (MQ=255)                                                                                                                                                                                                                                                                  gtgtGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGc             >  1:163690/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  gtgtGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGc             >  2:417881/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  gtgtGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTACCACAGGGTGACGc             >  2:474059/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  gtgtGGCGATTCACCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTATTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTAGATTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGc             >  2:106766/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  gtgtGGCGATTCACCTCG‑AAGCGCGCTGCCAGTCGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTAGCTTATCAGATAAAGTGCTCACGCAACCGTTGAGAGAAGAAATCCAGCAAGCTGACTGTGAAGATGATAAGCACCATCAGGGCACAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGAc               >  2:474036/1‑298 (MQ=255)                                                                                                                                                                                                                                                                              aCCTCG‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCt  >  1:352539/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                       GACGCAGGGTGCGCAGGTGGATGACGTTCTCCCCCTCGGTAATCCCCAGTGCGTCAGCGACGTGGCCGGACGCGGGGCGCAATACCGAAAGCAGTTTTTCGCTGGTGGGATGGCTGCCCTGATCCAGCAGATTCTGGCTAAAACGCGCCTGGGCGTTGAGCGGGTAATCGAACGGGCGCATCAGCACCAGCACGCCGACGCCCTGACGGCGCTGTACCCAGCCTTTTTCCACCAGTTGGTCGATGGCGCGGCGCAGGGTGTGGCGATTCACCTCA‑AAGCGCGCTGCCAGTTGCTGCTCGGCGGGAAGATAGTCGCCGCAGCGGTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCT  >  NC_000913/4322014‑4322581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑