Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,256,705	C→T	R23R (CGC→CGT)	dgkA →	diacylglycerol kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,256,705	0	C	T	97.8%	295.6 / NA	90	R23R (CGC→CGT)	dgkA	diacylglycerol kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (42/46);  minor base G (0/2);  total (42/48)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACCATGAGCTTTCCGGACGCGCAAAAGATATGGGATCCGCTGCGGTGCTGATTGCCATTATCGTCGCCGTGATTACCTGGTGCATTCTGTTATGGTCGCATTT  >  NC_000913/4256416‑4256998                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                      gCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGa                                                                                                                                                                                                                                                                                             <  2:394345/300‑1 (MQ=255)   ttAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGNAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATc                                                                                                                                                                                                                                                                                           <  1:483820/300‑1 (MQ=255)   ttAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATc                                                                                                                                                                                                                                                                                           <  1:11084/300‑1 (MQ=255)      aTGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGNTTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCAGAATTATCAAAGCTGCCGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAAc                                                                                                                                                                                                                                                                                        >  2:216742/1‑300 (MQ=255)        ggTAAATTCAGTAATTTTTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGa                                                                                                                                                                                                                                                                                      <  2:433995/300‑1 (MQ=255)        ggTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATTTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGTAAAGGTTTACGTGCTGCATGGATCAACGa                                                                                                                                                                                                                                                                                      <  1:256321/300‑1 (MQ=255)        ggTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGa                                                                                                                                                                                                                                                                                      <  2:149697/300‑1 (MQ=255)        ggTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGa                                                                                                                                                                                                                                                                                      <  1:256329/300‑1 (MQ=255)        ggTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGAGGGAAATCCTCTGGTTAATAATGCAAGTGCGCGGCAATGATAGCAGAAAGTCATGGGAAAGTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGACATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTGACGGGCTGCATGGATCAACGa                                                                                                                                                                                                                                                                                      <  2:453560/300‑1 (MQ=255)             aTTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGa                                                                                                                                                                                                                                                                                      >  1:216176/1‑295 (MQ=255)                    aaTTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGNCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTc                                                                                                                                                                                                                                                                            <  2:541283/298‑1 (MQ=255)                     aTTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGt                                                                                                                                                                                                                                                                         <  2:638463/300‑1 (MQ=255)                      tttGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTc                                                                                                                                                                                                                                                                        <  1:506457/300‑1 (MQ=255)                           agtaAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGaa                                                                                                                                                                                                                                                                   <  1:629135/300‑1 (MQ=255)                                     tGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCgg                                                                                                                                                                                                                                                         <  1:71732/300‑1 (MQ=255)                                          atccGGACAGAAGCGAGGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAtgg                                                                                                                                                                                                                                                    <  2:102403/298‑3 (MQ=255)                                          agctggACAGAAACGATGTGAAGCCTCTGGTTAATAAGACAAATGCGCGGCAAGGATAGCATAAAGTCATGGGAAATTCTGTGGGATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGGATTAGCAAAGCTGCTGGCTATTCCTGGAAAGGTTGACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAtgg                                                                                                                                                                                                                                                    <  2:101095/296‑3 (MQ=255)                                          aGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAttg                                                                                                                                                                                                                                                    <  1:514629/300‑1 (MQ=255)                                          aGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAt                                                                                                                                                                                                                                                      <  2:305238/298‑1 (MQ=255)                                          aGCCAGACATAAACGAGGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGAGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAttg                                                                                                                                                                                                                                                    <  2:102341/300‑1 (MQ=255)                                           gCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAttgt                                                                                                                                                                                                                                                   >  1:321109/1‑300 (MQ=255)                                              ggACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAATTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCCCATAATGTAAAGAGGTTCTTTCAATGGCCAATAATACCACTGGATGAACCCGAATTATCAAAGCTGCTTGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAttgttg                                                                                                                                                                                                                                                 <  1:98347/299‑1 (MQ=255)                                              ggACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAACTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAttgttg                                                                                                                                                                                                                                                 <  2:405867/299‑1 (MQ=255)                                               gacctAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCCAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGc                                                                                                                                                                                                                                               >  2:658821/5‑300 (MQ=255)                                               gACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCCCCCGACTTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGTGTAGTGGTAttgtt                                                                                                                                                                                                                                                  >  2:658822/1‑297 (MQ=255)                                               gACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGNCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAAAGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGc                                                                                                                                                                                                                                               >  1:135171/1‑300 (MQ=255)                                               gACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTAttgttg                                                                                                                                                                                                                                                 <  2:405865/298‑1 (MQ=255)                                                         atttAAAACCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATc                                                                                                                                                                                                                                     <  2:124762/297‑1 (MQ=255)                                                         aTGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTTTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATc                                                                                                                                                                                                                                     <  2:559811/300‑1 (MQ=255)                                                                    ctGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGCAGCGGTATTGTTGGCGGTGGTCATCGCCTgctggct                                                                                                                                                                                                                          >  2:247663/1‑300 (MQ=255)                                                                         taataaTGCAAATTCGCGGCAAGGATAGCATAAAGTCATGTGAACTTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAACTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCGTGGATCAACTAAGCGGCATTCCGGCAGTAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGAtg                                                                                                                                                                                                                     <  2:351762/300‑1 (MQ=255)                                                                               tGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAACAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAATCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATATGGAcg                                                                                                                                                                                                               <  1:514105/300‑1 (MQ=255)                                                                                        gctgCAAGGATAGCAGAAAGTCATTGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTAccc                                                                                                                                                                                                      <  1:383757/297‑1 (MQ=255)                                                                                               ggATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGtgct                                                                                                                                                                                               >  1:58759/1‑300 (MQ=255)                                                                                               ggATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAANAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGtgct                                                                                                                                                                                               >  2:495425/1‑300 (MQ=255)                                                                                               ggATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTAAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAANCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGtgct                                                                                                                                                                                               >  2:215746/1‑300 (MQ=255)                                                                                                      aGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATc                                                                                                                                                                                        <  1:436228/300‑1 (MQ=255)                                                                                                        aaaGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAg                                                                                                                                                                                      <  1:279313/300‑1 (MQ=255)                                                                                                                gggAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGa                                                                                                                                                                              <  1:57459/300‑1 (MQ=255)                                                                                                                gggAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGGGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGa                                                                                                                                                                              <  1:57567/300‑1 (MQ=255)                                                                                                                   aaaTTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGc                                                                                                                                                                           <  2:549168/300‑1 (MQ=255)                                                                                                                           tgGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCTGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGTtgatg                                                                                                                                                                   <  2:319665/300‑1 (MQ=255)                                                                                                                           tgGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGtgatg                                                                                                                                                                   <  1:379505/300‑1 (MQ=255)                                                                                                                                    cTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGaa                                                                                                                                                          >  2:48145/1‑300 (MQ=255)                                                                                                                                              gcgcgGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAAt                                                                                                                                                 <  1:39796/299‑1 (MQ=255)                                                                                                                                              gcgcgGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACACTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAAt                                                                                                                                                 <  2:302019/299‑1 (MQ=255)                                                                                                                                              gcgcgGCGCTACGCAAACCCGAATCATCAGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGCTCTTTCAATTGCCAACAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTACTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAAt                                                                                                                                                 <  2:69313/299‑1 (MQ=255)                                                                                                                                                  ggcgCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCTTGATGCTGGTGATGATTGTGGAAATCCTCAATAgcgc                                                                                                                                            <  2:678541/300‑1 (MQ=255)                                                                                                                                                  ggcgCGACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCGCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTTGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGATATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGTTGATGATTGTGGAAATCCTCAATAgcgc                                                                                                                                            <  2:316129/300‑1 (MQ=255)                                                                                                                                                   gcgcTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTTTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGcc                                                                                                                                           >  2:102597/1‑300 (MQ=255)                                                                                                                                                   gcgcTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGcc                                                                                                                                           >  2:148135/1‑300 (MQ=255)                                                                                                                                                   gcgcTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGcc                                                                                                                                           >  1:403366/1‑300 (MQ=255)                                                                                                                                                   gcgcTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTAGGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCATAAATAgcgc                                                                                                                                            >  2:421219/1‑299 (MQ=255)                                                                                                                                                   gcgcTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCAGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCATGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGcc                                                                                                                                           >  2:421417/1‑300 (MQ=255)                                                                                                                                                   gcgcCACGCAAACCCGAATCATCGGATTTAATGGTACACGGAGATTGACGATAATAATGTAAAAAGGTTCTTTCGATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCATGAAGGCGTAGCGGTATTGTTGTCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAgcgc                                                                                                                                            <  2:315974/299‑1 (MQ=255)                                                                                                                                                     gcTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCAGAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACTAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCGGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAgcgc                                                                                                                                            <  2:315965/297‑1 (MQ=255)                                                                                                                                                        aCGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGCCGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGa                                                                                                                                      >  1:539903/1‑300 (MQ=255)                                                                                                                                                        aCGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGa                                                                                                                                      >  1:539909/1‑300 (MQ=255)                                                                                                                                                          gCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGaa                                                                                                                                     >  1:16180/1‑299 (MQ=255)                                                                                                                                                             aaCCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCa                                                                                                                                  >  1:658048/1‑299 (MQ=255)                                                                                                                                                               cccGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCNATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGt                                                                                                                                >  1:144738/1‑299 (MQ=255)                                                                                                                                                                  gAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGTCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCTAAGCAGTgg                                                                                                                              <  2:88899/298‑1 (MQ=255)                                                                                                                                                                  gAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTgg                                                                                                                              <  1:632776/298‑1 (MQ=255)                                                                                                                                                                                 aCGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGCTTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCACTAGCGCCATCGAAGCAGTGGTTGACCGAATTGGctct                                                                                                             >  1:171274/1‑300 (MQ=255)                                                                                                                                                                                 aCGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGctct                                                                                                             >  1:83714/1‑300 (MQ=255)                                                                                                                                                                                 aCGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGAACGAATTGGctct                                                                                                             >  1:427611/1‑300 (MQ=255)                                                                                                                                                                                    gTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGaa                                                                                                          >  1:357534/1‑300 (MQ=255)                                                                                                                                                                                       cacTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATAc                                                                                                       >  2:2787/1‑300 (MQ=255)                                                                                                                                                                                        acTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTTGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGTTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATAc                                                                                                       <  2:68773/299‑1 (MQ=255)                                                                                                                                                                                         cTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTNCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACCa                                                                                                     >  1:499448/1‑300 (MQ=255)                                                                                                                                                                                         cTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCCATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACCa                                                                                                     >  1:259233/1‑300 (MQ=255)                                                                                                                                                                                         cTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACCa                                                                                                     >  1:53203/1‑300 (MQ=255)                                                                                                                                                                                                        aTAATGTAAAAAGGTTCNTTCAATGGCCAATAATACNACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACNATGAGCTTTCCGGAcg                                                                                      <  1:574656/300‑1 (MQ=255)                                                                                                                                                                                                             gTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACCATGAGCTTTCCGGACGCGCaa                                                                                 >  2:130784/1‑300 (MQ=255)                                                                                                                                                                                                                    ggTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACCATGAGCTTTCCGGACGCGCAAAAGatat                                                                          <  1:652561/300‑1 (MQ=255)                                                                                                                                                                                                                         tttCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACCATGAGCTTTCCGGACGCGCAAAAGATATGGGa                                                                      <  2:16180/299‑1 (MQ=255)                                                                                                                                                                                                                         tttCAATGGCCAATAANACCACTGGATTCACCCGANTTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACCATGAGCTTTCCGGACNCGCAAAAGATATGGGa                                                                      <  1:574103/299‑1 (MQ=255)                                                                                                                                                                                                                                 gCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACCATGAGCTTTCCGGACGCGCAAAAGATATGGGATCCGCTGc                                                              >  1:122560/1‑299 (MQ=255)                                                                                                                                                                                                                                   cAATAATACCACTGGATTCGCCCGAATTATTGAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCAGTGGCCATCGCATGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTACGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACCATGAGTGTTCCGGATGAGCAAAAGATATGGGATCCGCTGCGGt                                                           >  2:260660/1‑300 (MQ=255)                                                                                                                                                                                                                                      taataCCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACCATGAGCTTTCCGGACGCGCAAAAGATATGGGATCCGCTGCGGTGCt                                                        >  1:226318/1‑300 (MQ=255)                                                                                                                                                                                                                                           ccACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACCATGAGCTTTCCGGACGCGCAAAAGATATGGGATCCGCTGCGGTGCTGAtt                                                    >  1:543313/1‑299 (MQ=255)                                                                                                                                                                                                                                                     cACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACCATGAGCTTTCCGGACGCGCAAAAGATATGGGATCCGCTGCGGTGCTGATTGCCATTAtcgt                                         >  2:314867/1‑300 (MQ=255)                                                                                                                                                                                                                                                              tctCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCAATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACCATGAGCTTTCCGGACGCGCAAAAGATATGGGATCAGCTGCAGTGCTGCTTGCCATTAtcgtagaagtgat                                >  2:269925/3‑291 (MQ=255)                                                                                                                                                                                                                                                                     gctgttGGCTATTCATGGAAAGATTTAAGTGCTGCATGGATCAACGAAGCGGCATTCAGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCTGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTAAATCGCGCAATCGAAGCAGTGGTTGACCGAATTGGCCCTGCATACCATGAGCTTTACGGACGCGCACAAGATATGGGATCCGCTGCGATGCTGCTTGCCATTCTCGTCGCCGCGATTAc                             >  2:325733/1‑296 (MQ=255)                                                                                                                                                                                                                                                                     gctgctGGCTATTCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACCATGAGCTTTCCGGACGCGCAAAAGATATGGGATCCGCTGCGGTGCTGATTGCCATTATCGTCGCCGTGATTACCt                           >  2:325110/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                ttCCTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACCATGAGCTTTCCGGACGCGCAAAAGATATGGGATCCGCTGCGGTGCTGATTGCCATTATCGTCGCCGTGATTACCTGGTGCATTCTGtt              >  1:472195/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   cTGGAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACCATGAGCTTTCCGGACGCGCAAAAGATATGGGATCCGCTGCGGTGCTGATTGCCATTATCGTCGCCGTGATTACCTGGTGCATTCTGTTAt            >  1:165659/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                      gAAAGGTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACCATGAGCTTTCCGGACGCGCAAAAGATATGGGATCCGCTGCGGTGCTGATTGCCATTATCGTCGCCGTGATTACCTGGTGCATTCTGTTATGGTc        <  1:216742/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                          ggTTTACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACCATGAGCTTTCCGGACGCGCAAAAGATATGGGATCCGCTGCGGTGCTGATTGCCATTATCGTCGCCGTGATTACCTGGTGCATTCTGTTATGGTCGCAt    >  1:94195/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            tttACGTGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGTTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACCATGAGCTTTCCGGACGCGCAAAAGATATGGGATCCGCTGCGGTGCTGATTGCCATTATCGTCGCCGTGATTACCTGGTGCATTCTGTTATGGTCGCAttt  >  2:349381/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                      GCTTAATGGTAAATTCAGTAATTTGTAGTAAATTCGTGGCCAGCCGGACATAAACGATGTAAAGCCTCTGGTTAATAATGCAAATGCGCGGCAAGGATAGCAGAAAGTCATGGGAAATTCTGTGGTATCCGCTCATGTTTCGCGCGGCGCTACGCAAACCCGAATCATCGGATTTAACGGTACACTGATATTGACGCTCATAATGTAAAAAGGTTCTTTCAATGGCCAATAATACCACTGGATTCACCCGAATTATCAAAGCTGCTGGCTATTCCTGGAAAGGTTTACGCGCTGCATGGATCAACGAAGCGGCATTCCGTCAGGAAGGCGTAGCGGTATTGTTGGCGGTGGTCATCGCCTGCTGGCTGGATGTGGACGCGATTACCCGCGTGCTGCTTATCAGCTCCGTGATGCTGGTGATGATTGTGGAAATCCTCAATAGCGCCATCGAAGCAGTGGTTGACCGAATTGGCTCTGAATACCATGAGCTTTCCGGACGCGCAAAAGATATGGGATCCGCTGCGGTGCTGATTGCCATTATCGTCGCCGTGATTACCTGGTGCATTCTGTTATGGTCGCATTT  >  NC_000913/4256416‑4256998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑