Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,138,436 C→T L200L (CTG→CTA)  gldA ← L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,138,4360CT98.1% 179.2 / NA 52L200L (CTG→CTA) gldAL‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (34/17);  minor base G (0/1);  total (34/18)
Fisher's exact test for biased strand distribution p-value = 3.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCCAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGT  >  NC_000913/4138141‑4138730
                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                      
gggCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGATCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCAGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGATTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAgc                                                                                                                                                                                                                                                                                                     >  2:403368/1‑299 (MQ=255)
   cAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCa                                                                                                                                                                                                                                                                                                  >  1:2031/1‑299 (MQ=255)
   cAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACTGCGTGCGCCGCAGCCAGACCACCAATTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCa                                                                                                                                                                                                                                                                                                  >  1:2246/1‑299 (MQ=255)
   cAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACTGCGTGCGCCGCAGCCAGACCAACACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCa                                                                                                                                                                                                                                                                                                  >  1:2260/1‑299 (MQ=255)
           gTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTNAAATAGGTGTTCGCTTCAATCACGCGCTCCAGNGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTg                                                                                                                                                                                                                                                                                         <  2:513230/300‑1 (MQ=255)
           gTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTg                                                                                                                                                                                                                                                                                         <  2:621662/300‑1 (MQ=255)
                                     ttCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTcgc                                                                                                                                                                                                                                                               >  1:671002/1‑300 (MQ=255)
                                               aGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACga                                                                                                                                                                                                                                                     <  1:600621/300‑1 (MQ=255)
                                                        aGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCa                                                                                                                                                                                                                                            >  1:170065/1‑300 (MQ=255)
                                                           gTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCANAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCAtggt                                                                                                                                                                                                                                                                     >  1:494933/1‑272 (MQ=255)
                                                           gTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCAcgc                                                                                                                                                                                                                                         >  1:392487/1‑300 (MQ=255)
                                                           gTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAANCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCAtggt                                                                                                                                                                                                                                                                     <  2:494933/272‑1 (MQ=255)
                                                             aCCGAATGCCACTTTTTCACCTTGATAATAGTGATGCGCATCCTGGATAGCGGTCAGGCCATTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCCCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATNCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTNTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACgcgc                                                                                                                                                                                                                                       <  2:511300/300‑1 (MQ=255)
                                                                                    gATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCAt                                                                                                                                                                                                                >  1:674927/1‑300 (MQ=255)
                                                                                    gATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCAGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCAt                                                                                                                                                                                                                >  1:674948/1‑300 (MQ=255)
                                                                                    gATAATAGTGATGCGCGTCCGGGATAGCGGCCAGGCCGTTCTGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCAATACATGCTGTTCGGCAGCAAGCATTGCTTTTTCGCATTCTTACAGTAGGGTGTTGTAGCACAGTTCAGCAAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGACATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACAAGGTTGCCAGCGCa                                                                                                                                                                                                                 >  2:27764/1‑299 (MQ=255)
                                                                                                  gcgTCCGGGATAGCGGTCAGGCCGTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACccgcc                                                                                                                                                                                                  >  1:15109/1‑300 (MQ=255)
                                                                                                  gcgTCCGGGATAGCGGTCAGGCCGTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAAACAGGTTGCAAGCGCATCGCCGATACccgcc                                                                                                                                                                                                  >  1:394234/1‑300 (MQ=255)
                                                                                                    gTCCGGGATAGCGGTCAGGCCGTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCcgccgc                                                                                                                                                                                                >  2:237753/1‑300 (MQ=255)
                                                                                                           aTAGCGGTCAGGCCGTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGNTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGa                                                                                                                                                                                         <  1:137127/300‑1 (MQ=255)
                                                                                                                    aGGCCGTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAgg                                                                                                                                                                                 <  2:476646/299‑1 (MQ=255)
                                                                                                                         gTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGcc                                                                                                                                                                           >  1:102310/1‑300 (MQ=255)
                                                                                                                         gTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCAAAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGcc                                                                                                                                                                           >  2:626053/1‑300 (MQ=255)
                                                                                                                         gTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTATTCCAGCAGGGTGTTGTAGCATAGTTCAGCAAGTGCTAGTGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCAGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCAAGCGCATCGACGATACCCGAAGCTAACAGAAGTGCAGGTGCGcc                                                                                                                                                                           >  2:66281/1‑300 (MQ=255)
                                                                                                                            aTGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGNCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCAAGc                                                                                                                                                                        >  2:527494/1‑300 (MQ=255)
                                                                                                                                                    accaccACTTTCAAAACCAACACCGCTCAAAGAGGTGTTCGCTTCAATCACGCGCTCCAGTGCCGGGGTCATTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTATCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCAtggt                                                                                                                                                                                                                                                                     <  2:611947/183‑1 (MQ=255)
                                                                                                                                                                aaaaACAATACCGCTCAGATAGGTGTTCGTTTCAATCACTCGCTCTAGCGCCGTATTCACTACATGCTGTGCTGCAGCAGGCATCACTTGTTTGCCTCCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCATTGCGAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCatactc                                                                                                                                         <  2:384753/295‑4 (MQ=255)
                                                                                                                                                                  aaCCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTAt                                                                                                                                  >  2:601148/1‑300 (MQ=255)
                                                                                                                                                                      aaCACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTAttt                                                                                                                                <  2:614001/298‑1 (MQ=255)
                                                                                                                                                                         accacTCAAATAAGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATTTTGTTCGGTAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTTTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCaa                                                                                                                           <  2:76008/296‑1 (MQ=255)
                                                                                                                                                                              gcgagatgtgtataagagacagcACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAAcagca                                                                                                                      >  1:464068/23‑300 (MQ=255)
                                                                                                                                                                               cAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCAAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAAcaaca                                                                                                                      >  2:17191/1‑296 (MQ=255)
                                                                                                                                                                                    aGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGc                                                                                                                <  2:170065/300‑1 (MQ=255)
                                                                                                                                                                                                  aTCACGCGCTCCAGCGCCGTAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTTTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGTTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCAccc                                                                                                  <  1:412765/300‑1 (MQ=255)
                                                                                                                                                                                                  aTCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCAccc                                                                                                  <  1:237753/300‑1 (MQ=255)
                                                                                                                                                                                                                 gcCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTTTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATa                                                                                   <  2:431857/300‑1 (MQ=255)
                                                                                                                                                                                                                 gcCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATa                                                                                   <  1:509374/300‑1 (MQ=255)
                                                                                                                                                                                                                    ggAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAAc                                                                                 <  1:5710/299‑1 (MQ=255)
                                                                                                                                                                                                                     gAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACa                                                                                >  1:604117/1‑299 (MQ=255)
                                                                                                                                                                                                                          aCTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCAGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAAACAGGTTGCCAGCGCATCGACGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCCGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAAt                                                                          >  1:229749/1‑300 (MQ=255)
                                                                                                                                                                                                                                                      cTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCNACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGa                                              >  1:490482/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                           gggTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGTGATAGTCGGTGCGATCGCTaac                          >  2:136923/1‑297 (MQ=255)
                                                                                                                                                                                                                                                                           gggTGTTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTAcc                          >  2:242381/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                             gtgtTGTAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGa                       >  1:160842/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                               gttgtAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAac                     >  2:350055/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                ttgtAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAACCTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGaa                      >  2:627835/1‑298 (MQ=255)
                                                                                                                                                                                                                                                                                ttgtAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCAc                                                                 <  1:268039/255‑1 (MQ=255)
                                                                                                                                                                                                                                                                                ttgtAGCACAGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCAc                                                                 >  2:268039/1‑255 (MQ=255)
                                                                                                                                                                                                                                                                                        caGTTCAGCCAGTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGaa            >  1:555615/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                   gTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGTGATAGTCGGTGCGATCGCTACCGGAACACCCATGCAATGTGCCAGt  >  2:424810/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                                                   gTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGt  >  1:94861/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                                                   gTGCTAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGt  >  2:27248/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                      
GGGCAGCTACGGTTTCGATTTCCTCCACCGGCGCATTTTCCAGAACCAGCTGCGTCAGCGTACCGAATGCCACTTTTTCACCGTGATAATAGTGATGCGCGTCCGGGATAGCGGTCAGGCCGTTATGCACTGCGTGCGCCGCAGCCAGACCACCACTTTCAAAACCAACACCGCTCAAATAGGTGTTCGCTTCAATCACGCGCTCCAGCGCCGGAGTCACTACATGCTGTTCGGCAGCAAGCATCGCTTTTTCGCCTTCTTCCAGCAGGGTGTTGTAGCACAGTTCAGCCAGTGCCAGCGCAGCCTGGGTGCACTTGCCGCCCGCCATGGTGGTCGCGCCGCTACGAGAGCAGGCACGCGCTTCAAACCAGGTTGCCAGCGCATCGCCGATACCCGCCGCTAACAGACGTGCAGGTGCGCCAGCGACGATTTTGGTGTCGACAATGACCATATTCGGGTTATTTGGCAACAGCAGATAGCGGTCAAACTCACCCTCATCGGTGTAGATAACAGACAATGCGCTGCACGGTGCATCGGTAGAGGCGATAGTCGGTGCGATCGCTACCGGAACACCCATGAAATGTGCCAGT  >  NC_000913/4138141‑4138730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑