Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,095,026	G→A	D319D (GAC→GAT)	rhaA ←	L‑rhamnose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,095,026	0	G	A	100.0%	194.5 / NA	53	D319D (GAC→GAT)	rhaA	L‑rhamnose isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/32);  total (21/32)

TTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCAACGATTTCTGCTCTTCCAGCAGTGCCAGACGCGCAGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATACCCCATGTAAAACTCATTGGAGCCAACCGTGTAGCTCTCTGCGCCAATGCCAAACAATTTGCTCTCAAC  >  NC_000913/4094747‑4095310                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                             tttctCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCAACGATTTCTGCTCTTCCAGCAGTGCCAGACGCGCAGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGcac                                                                                                                                                                                                                                                                          <  1:559582/300‑1 (MQ=255)                    ctctCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCAACGATTTCTGCTCTTCCAGCAGTGCCAGACGCGCAGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGcc                                                                                                                                                                                                                                                       >  1:616914/1‑300 (MQ=255)                    ctctCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCAACGATTTCTGCTCTTACAGCAGTGCCAGACGCGCAGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTAATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCATAATCTCACTGGCAATTGc                                                                                                                                                                                                                                                        >  2:290956/1‑299 (MQ=255)                      ctcCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCAACGATTTCTGCTCTTCCAGCAGTGCCAGACGCGCAGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGcat                                                                                                                                                                                                                                                      >  1:396164/1‑297 (MQ=255)                      ctcCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCAACGATTTCTGCTCTTCCAGCAGTGCCAGACGCGCAGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCt                                                                                                                                                                                                                                                      >  1:395484/1‑299 (MQ=255)                                                tATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCAACGATTTCTGCTCTTCCAGCAGTGCCAGACGCGCAGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGt                                                                                                                                                                                                                           <  2:392659/300‑1 (MQ=255)                                                      ggcGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCAACGATTTCGGCTCTTCCAGCAGTGCCAGACGCGCAGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACACACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGc                                                                                                                                                                                                                     <  2:407583/298‑1 (MQ=255)                                                               aaTACATTTCCCAGACCGCCTGCCACGGCAACGATTTCTGCTCTTCCAGCAGTGCCAGACGCGCAGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCANGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAg                                                                                                                                                                                                             <  2:488767/299‑1 (MQ=255)                                                                              ccGCCTGCCACGGCAACGATTTCTGCTCTTCCAGCAGTGCCAGACGCGCAGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGa                                                                                                                                                                                             >  1:2855/1‑300 (MQ=255)                                                                                                           tCCAGCAGTGCCAGACGCGCAGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGTGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGc                                                                                                                                                                <  2:241365/300‑1 (MQ=255)                                                                                                           tCCAGCAGTGCCAGACGCGCAGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGc                                                                                                                                                                <  2:455043/300‑1 (MQ=255)                                                                                                           tCCAGCAGTGCCAGACGCGCAGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGc                                                                                                                                                                <  1:74180/300‑1 (MQ=255)                                                                                                              agcagTGCCAGACGCGCAGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCAt                                                                                                                                                              <  1:105415/299‑1 (MQ=255)                                                                                                                                gtgtAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTNACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTc                                                                                                                                            >  2:219744/1‑299 (MQ=255)                                                                                                                                gtgtAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTc                                                                                                                                            >  1:420235/1‑299 (MQ=255)                                                                                                                                                          gcgcAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGt                                                                                                                  >  1:168392/1‑299 (MQ=255)                                                                                                                                                            ttcacTCAGCGGTAGGTTCCAGCGACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCg                                                                                                                           <  2:123990/284‑1 (MQ=255)                                                                                                                                                              aGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTNGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGccc                                                                                                              <  2:493183/299‑1 (MQ=255)                                                                                                                                                               gCTCAGCGGTAGGTTCCAGCAATGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTNAAGGCCGATATGCACCCGATCAAACAGATCGTNACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGATGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGCCGGGTGGAAGTGccc                                                                                                              >  2:519006/1‑298 (MQ=255)                                                                                                                                                               gCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTAAGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGt                                                                                                                  >  2:99664/1‑294 (MQ=255)                                                                                                                                                                  cAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGt                                                                                                         >  1:284298/1‑300 (MQ=255)                                                                                                                                                                    gCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCACGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTAGGTTTCATCATCCAGCAGCCCTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATAGAGGCGGAAATCTTGTCGGAAATCACTTAAGTCGGGTGGAAGTGCCCGGCGTcc                                                                                                       >  2:92850/1‑300 (MQ=255)                                                                                                                                                                          gtttCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCTGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGACCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGcac                                                                                                 <  2:339377/298‑1 (MQ=255)                                                                                                                                                                          ggTTCCAGCAACGCACGNAGCAGGGCTTTTTTCATANTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTNCCCGGCGTCCAGGcac                                                                                                 <  1:563406/300‑1 (MQ=255)                                                                                                                                                                          ggTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGcac                                                                                                 <  2:612946/300‑1 (MQ=255)                                                                                                                                                                                      gcacgcgGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGACGTGCCCGGCGTCCAGGCACAGCGCAGTCt                                                                                       >  2:620196/1‑298 (MQ=255)                                                                                                                                                                                      gcacgcaGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCt                                                                                       >  1:652999/1‑298 (MQ=255)                                                                                                                                                                                        acgcaGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCTATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCAATTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTggc                                                                                    >  1:665566/1‑299 (MQ=255)                                                                                                                                                                                          gcagcaGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTTGAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGTTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTggcgg                                                                                  <  2:16305/299‑1 (MQ=255)                                                                                                                                                                                            agcagGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCcggcgg                                                                                  <  2:71291/297‑1 (MQ=255)                                                                                                                                                                                                     tttttCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATAc                                                                      <  1:324796/300‑1 (MQ=255)                                                                                                                                                                                                       tttCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATAccc                                                                    <  1:592959/300‑1 (MQ=255)                                                                                                                                                                                                        ttCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAAATCGTGACGCACAATCTCACTGGCAATTGCATGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCAAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGAGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATAcc                                                                     >  2:289539/1‑298 (MQ=255)                                                                                                                                                                                                                       ccAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATACCCCATGTAAAACTCAtt                                                     >  2:388322/1‑299 (MQ=255)                                                                                                                                                                                                                                 actcgGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATACCCCATGTAAAACTCATTGGAGCCAAcc                                           <  1:359376/296‑1 (MQ=255)                                                                                                                                                                                                                                 aCGCGGCAATGCGGTTGATAGATGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCNCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATACCCCATGTAAAACTCATTGGAGCCAACCg                                          <  2:227861/300‑1 (MQ=255)                                                                                                                                                                                                                                 aCGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCNCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATACCCCATGTAAAACTCATTGGAGCCAACCg                                          <  2:211008/300‑1 (MQ=255)                                                                                                                                                                                                                                        aaTGCGGTGGATAGAGGCAGCGAAGAAGTCAATGCCGATATACACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGTGTTTCATCATCCAGCAGCACGACGGGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATACCCCATGTAAAACTCATTGGAGCCAACCGTGTAg                                     <  2:260684/298‑1 (MQ=255)                                                                                                                                                                                                                                         aTGCGGTTGATATAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATACCCCATGTAAAACTCATTGGAGCCAACCGTGTAGctc                                  <  2:672078/300‑1 (MQ=255)                                                                                                                                                                                                                                         aTGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACGGAGCGTGACGCACAATCTCACTTGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACTTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCTGGTGGAAGTGCCCTGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATACCCCATGTAAAACTCATTGGAGCCAACCGTGTAg                                     <  2:260718/297‑1 (MQ=255)                                                                                                                                                                                                                                         aTGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATACCCCATGTAAAACTCATTGGAGCCAACCGTGTAGctc                                  <  1:90114/300‑1 (MQ=255)                                                                                                                                                                                                                                              gTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCAATTAAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATACCACATGTAAAACTCATTGGAGCCAACCGTGTAGctatct                               >  2:19531/1‑298 (MQ=255)                                                                                                                                                                                                                                                tGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCTTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATACCCCATGTAAAACTCATTGGAGCCAACCGTGTAGCTCTCTgcgc                           <  2:155135/300‑1 (MQ=255)                                                                                                                                                                                                                                                 gATAGAGGCATCGAAGGAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGTCAATTGCCTGGGTTTCATCATCAAGCAGCACTACTTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCACGGCTGCGGAAATCTGGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGTTGGCATACCCCATGTAAAACTCATTGGAGCCAACCGTGTAGCTCTCGGCGcc                          <  1:453095/300‑1 (MQ=255)                                                                                                                                                                                                                                                 gATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCAAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATACCCCATGTAAAACTCATTGGAGCCAACCGTGTAGCTCTCTGCGcc                          <  1:453104/300‑1 (MQ=255)                                                                                                                                                                                                                                                  aTAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTTGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATACCCCATGTAAAACTCATTGGAGCCAACCGTGTAGCTCTCTGCGCCa                         <  2:314457/300‑1 (MQ=255)                                                                                                                                                                                                                                                      atacagcGAAGAAGTCGAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACGATCTCACTGTCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGNTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGTTGGCATACTCCATGTAAAACTCATTGGAGCCAACCGTTTAGCTCTCTgctcc                          <  2:208078/289‑4 (MQ=255)                                                                                                                                                                                                                                                             gaagaaGTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATACCCCATGTAAAACTCATTGGAGCCAACCGTGTAGCTCTCTGCGCCAATGCCAAACaa              >  2:276344/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  agTCAAGGCCTATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATACCCCATGTAAAACTCATTGGAGCCAACCGTGTAGCTCTCTGCGCCAATGCCAAACAATTTGc         >  1:65671/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  agTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATACCCCATGTAAAACTCATTGTAGCCAACCGTGTAGCTCTCTGCGCCAATGCCAAACAATTTGc         >  1:553814/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  agTCAAGGCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATACCCCATGTAAAACTCATTGGAGCCAACCGTGTAGCTCTCTGCGCCAATGCCAAACAATTTGc         <  2:605377/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         gCCGATATGCACCCGATCAAACAGATCTTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATACCCCATGTAAAACTCATTGGAGCCAACCGTGTAGCTCTCTGCGCCAATGCCAAACAATTTGCTCTCAAc  <  2:396164/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         gCCGATATGCACCCGATCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATACCCCATGTAAAACTCATTGGAGCCAACCGTGTAGCTCTCTGCGCCAATGCCAAACAATTTGCTCTCAAc  <  2:395484/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                             TTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGGCAATACATTTCCCAGACCGCCTGCCACGGCAACGATTTCTGCTCTTCCAGCAGTGCCAGACGCGCAGTGTAATCGCCCGCCGCTTCCAGCTTGCGCAGCTCAGCGGTAGGTTCCAGCAACGCACGCAGCAGGGCTTTTTTCATATTGCGTGTACCAATGACCCACGCGGCAATGCGGTTGATAGAGGCATCGAAGAAGTCAAGGCCGATATGCACCCGGTCAAACAGATCGTGACGCACAATCTCACTGGCAATTGCCTGGGTTTCATCATCCAGCAGCACTACGTGATCGCTGTCCCAGCGAACCGGACGGCTGACGTGCAGCAGCAACTGCGGCACATACAGCATGGCGGCGGAAATCTTGTCGGAAATCACTTCAGTCGGGTGGAAGTGCCCGGCGTCCAGGCACAGCGCAGTCTGGCGGCTGGTGGCATACCCCATGTAAAACTCATTGGAGCCAACCGTGTAGCTCTCTGCGCCAATGCCAAACAATTTGCTCTCAAC  >  NC_000913/4094747‑4095310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑