Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,737,783	C→T	T96I (ACC→ATC)	malS →	alpha‑amylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,737,783	0	C	T	100.0%	213.5 / NA	63	T96I (ACC→ATC)	malS	alpha‑amylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/31);  total (32/31)

GCCATGAAACTCGCCGCCTGTTTTCTGACACTCCTTCCTGGCTTCGCCGTTGCCGCCAGCTGGACTTCTCCGGGGTTTCCCGCCTTTAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAACCCGCTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACACATCCGCCCCTTTCGACTGGCATAACGCCACGGTTTACTT  >  NC_000913/3737494‑3738074                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                    gCCATGAAACTCGCCGCCTGTTTTCTGACACTCCTTCCTGGCTTCGCCGTTGCCGCCAGCTGGACTTCTCCGGGGTTNCCCGCCTTTAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCgg                                                                                                                                                                                                                                                                                            <  1:498090/299‑1 (MQ=255)           tCGCCGCCTGTTTTCTGACACTCCTTCCTGGCTTCGCCGTTGCCGCCAGCTGGACTTCTCCGGGGTTTCCCGCCTTTAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACAt                                                                                                                                                                                                                                                                                 <  1:33190/300‑1 (MQ=255)           tCGCCACCTGTTTTCTGACGCTCCTTCCTTTCTTCGCCGCTGCCGCCAGCTGTACTTCTCCTGGGTTTCCCGCCTTTAGCGAACATTGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAGTCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGTCAACAt                                                                                                                                                                                                                                                                                 <  2:252479/300‑1 (MQ=255)                          tGACACTCCTTCCTGGCTTCGCCGTTGCCGCCAGCTGGACTTCTCCGGGGTTTCCCGCCTTTAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTtccatcc                                                                                                                                                                                                                                                                  <  1:394168/300‑1 (MQ=255)                               cTCCTTCCTGGCTTCGCCGTTGCCGCCAGCTGGACTTCTCCGGGGTTTCCCGCCTTTAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCCATGGCGATTGTTCAGGGACGGCGAATATACGCTGCCAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGa                                                                                                                                                                                                                                                               >  1:285346/1‑298 (MQ=255)                               cTCCTTCCTGGCTTCGCCGTTGCCGCCAGCTGGACTTCTCCGGGGTTTCCCGCCTTTAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAAc                                                                                                                                                                                                                                                             >  1:286406/1‑300 (MQ=255)                                           ttCGCCGTTGCCGCCAGCTGGACTTCTCCGGGGTTTCCCGCCTTTAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCg                                                                                                                                                                                                                                                 <  1:433211/300‑1 (MQ=255)                                                          aGCTGGACTTCTCCGGGGTTTCCCGCCTTTAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTc                                                                                                                                                                                                                                  <  1:589180/300‑1 (MQ=255)                                                                aCTTCTCCGGTGTTTCCCGCCTTTAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAANACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGTTAGCAAGCCTGGTCCGTGaa                                                                                                                                                                                                                            <  2:222513/300‑1 (MQ=255)                                                                    ctcCGGGGTTTCCCGCCTTTAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCCATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCTGTGAATGc                                                                                                                                                                                                                         >  2:459302/1‑299 (MQ=255)                                                                     tcctgggTTTCCCGCCTTTAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGcc                                                                                                                                                                                                                        <  2:115869/295‑1 (MQ=255)                                                                                      ttAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTAc                                                                                                                                                                                                      >  1:515342/1‑300 (MQ=255)                                                                                           gAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTc                                                                                                                                                                                                 <  1:201700/300‑1 (MQ=255)                                                                                                ggggACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGt                                                                                                                                                                                            >  1:673936/1‑300 (MQ=255)                                                                                                  ggACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGTTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAACAAGCCTGGTACGTGAATACCCGAAATGGGATGGATTACCGATcaccgt                                                                                                                                                                                            >  2:360972/1‑295 (MQ=255)                                                                                                  ggACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGACTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGt                                                                                                                                                                                            >  1:639200/1‑298 (MQ=255)                                                                                                  ggACAAGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCAACTAACGCTAAATTCTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCAAACATTGATGATTTACATCCAGAACGCCGCCGAACCGGTAGCAAGCATGGTCCGTGGATGCCCGAAGTGGGATGGATTAc                                                                                                                                                                                                      >  2:360982/1‑288 (MQ=255)                                                                                                      aGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCATGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATg                                                                                                                                                                                       <  1:366492/299‑1 (MQ=255)                                                                                                      aGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATg                                                                                                                                                                                       <  2:79797/299‑1 (MQ=255)                                                                                                      aGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATg                                                                                                                                                                                       <  1:349911/299‑1 (MQ=255)                                                                                                        gcaCATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGAttt                                                                                                                                                                                      >  2:375792/3‑296 (MQ=255)                                                                                                        gAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTAAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCAGAAATGGGATGGATTACCGCTCACAGTGGATGTCa                                                                                                                                                                                    >  1:202427/1‑300 (MQ=255)                                                                                                           cATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAgc                                                                                                                                                                                  >  1:159473/1‑299 (MQ=255)                                                                                                             tttGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTNAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGcc                                                                                                                                                                               >  2:500098/1‑300 (MQ=255)                                                                                                             tttGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGcc                                                                                                                                                                               >  2:431698/1‑300 (MQ=255)                                                                                                                       aCGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTccc                                                                                                                                                                       >  1:332807/1‑298 (MQ=255)                                                                                                                              gTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAgc                                                                                                                                                              >  1:669733/1‑300 (MQ=255)                                                                                                                                 gCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCATCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGACCCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAgccgc                                                                                                                                                           >  2:632363/1‑300 (MQ=255)                                                                                                                                  cccAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAgccgcc                                                                                                                                                          >  1:662748/1‑300 (MQ=255)                                                                                                                                      aaGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCCAATAGACATCTGCTCCGATACGCAAAAATTGATGATTTCCATCCAAAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGCTTAACGCTCACAGTGGATGTCAGCGCCACTTTCCAGGAAGGAGCCGCCGTAc                                                                                                                                                      >  2:286254/1‑300 (MQ=255)                                                                                                                                        ggTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACgg                                                                                                                                                    <  2:668390/300‑1 (MQ=255)                                                                                                                                                tCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACTGGAttatta                                                                                                                                            <  1:252565/300‑1 (MQ=255)                                                                                                                                                tCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGAttatta                                                                                                                                            <  1:252387/300‑1 (MQ=255)                                                                                                                                                   aCTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCATGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGAttatta                                                                                                                                            <  1:252381/297‑1 (MQ=255)                                                                                                                                                     tAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGTCGATTGTTCATGGACGGCGAATATACGCTGCANATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATTGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTAcagcc                                                                                                                                       <  2:215281/300‑1 (MQ=255)                                                                                                                                                           tAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTACAGCCAGCaa                                                                                                                                  <  2:137305/299‑1 (MQ=255)                                                                                                                                                                         cAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTACCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCAGGAAGGAGCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTgaa                                                                                                                    >  2:265286/1‑299 (MQ=255)                                                                                                                                                                           gTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTgaagaa                                                                                                                 >  1:327689/1‑300 (MQ=255)                                                                                                                                                                           gTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATACAGAACGCAGCCGAACCGGTAGCAAGCATGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTAAAGCCAACAAATTGCGCTAGTgaagaa                                                                                                                 >  2:614950/1‑300 (MQ=255)                                                                                                                                                                            tGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCTGAAGGAGCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAAc                                                                                                                <  2:308919/300‑1 (MQ=255)                                                                                                                                                                                    gCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGNCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAaat                                                                                                        >  1:136502/1‑300 (MQ=255)                                                                                                                                                                                    gCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGNCAGCGCCACTTTCCCGGAAGGAGCCGCAGTACGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAaat                                                                                                        >  1:486924/1‑300 (MQ=255)                                                                                                                                                                                                gATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACTGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAAcc                                                                                            <  2:456962/300‑1 (MQ=255)                                                                                                                                                                                                      aCTCAATCAGATGCTTTACCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAACCCGCTGc                                                                                      <  1:617662/300‑1 (MQ=255)                                                                                                                                                                                                                    tttCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAACCCGCTGCCACCAGCAACGGtt                                                                        <  1:516437/300‑1 (MQ=255)                                                                                                                                                                                                                                                     aaTGGCGATTGTTCATGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGTAAGGAGCCGCCGTACTGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAACCCGCTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGacac                                         <  2:310475/298‑1 (MQ=255)                                                                                                                                                                                                                                                               gTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAACCCGCTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACACATCCGCCCCttt                             >  1:171004/1‑300 (MQ=255)                                                                                                                                                                                                                                                               gTTCAGGGACGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCAGGAAGGAGCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAACCCGCTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACACATCCGCCCCttt                             >  1:171283/1‑300 (MQ=255)                                                                                                                                                                                                                                                               gTTCAGGGACGGCGAATATACGCTGCAAATAGAAATCCGCTCAGGTACGCAAAAATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCAGGAGGGAGCCGCCGTACGGGGTTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAACCCACTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAAGAACTGACACAt                                       >  2:44068/1‑290 (MQ=255)                                                                                                                                                                                                                                                                        cGGCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAACCCGCTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACACATCCGCCCCTTTCGACTGGCa                    >  1:325919/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          gCGAATATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAACCCGCTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACACATCCGCCCCTTTCGACTGGCATa                  >  1:41897/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             aaTATACGCTGCAAATAGACATCCGCTCCGTGACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAATCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCGCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACTGGATTACTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAACCCGCTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACACATCCGCCCCTTTCGACTGGCATAACg               <  2:285346/300‑1 (MQ=255)                                                                                                                                                                                                                                                                             aaTATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGTTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGTAAGGAGCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAACCCGCTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACACATCCGCCCCTTTCGACTGGCATAACg               <  2:286406/300‑1 (MQ=255)                                                                                                                                                                                                                                                                             aaTATACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTACAGCCAGCAAATTTCGATAGTGAAGAACGGTCAAATAATGTTACAACCCACTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACACATCCGCCCCTTTCGACTGGCATAACg               <  2:143393/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              atatACGCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAACCCGCTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACACATCCGCCCCTTTCGACTGGCATAACGc              <  1:265286/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              atatACACTGCAAATAGGCATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACTGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAACCCGCTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACACATCCGCCCCTTTCGACTGGCATAACGc              <  2:2999/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                    gCTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAACCCGCTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACACATCCGCCCCTTTCGACTGGCATAACGCCACGGt        <  2:669733/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     cTGCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAACCCGCTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACACATCCGCCCCTTTCGACTGGCATAACGCCACGGtt       >  1:402844/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                       gCAAATAGACGTCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGGTGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAACCCGCTGCCACCAGAAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACACATCCGCCCCTTTCGACTGGCATAACGCCACGGTTTa     >  2:474832/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                       gCAAATAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAAACCGCTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACACATCCGCCCCTTTCGAATGGCATAACGCCACGGTTTa     >  2:307413/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         aaaTAGACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTACAGNCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAACCCGCTGCCACCAGCAACGGTTTACTCATGCTGGAACGGGCAGAAACTGACACATCCGTCCCTTTCGACTGGCCTAACGCCACGGTTTACt   >  2:227938/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                          aaTATACATCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATTGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACTGGATTATTACANCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAACCCGCTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACACATCCGCCCCTTTCGACTGGCATAACGCCACGGTTTACtt  <  2:480188/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                          aaTAGACATCCTCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATTGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTACANCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAACCCGCTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACACATCCGCCCCTTTCGACTGGCATAACGCCACGGTTTACtt  <  2:480002/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                    GCCATGAAACTCGCCGCCTGTTTTCTGACACTCCTTCCTGGCTTCGCCGTTGCCGCCAGCTGGACTTCTCCGGGGTTTCCCGCCTTTAGCGAACAGGGGACAGGAACATTTGTCAGCCACGCGCAGTTGCCCAAAGGTACGCGTCCACTAACGCTAAATTTTGACCAACAGTGCTGGCAGCCTGCGGATGCGATAAAACTCAATCAGATGCTTTCCCTGCAACCTTGTAGCAACACGCCGCCTCAATGGCGATTGTTCAGGGACGGCGAATATACGCTGCAAATAGACACCCGCTCCGGTACGCCAACATTGATGATTTCCATCCAGAACGCCGCCGAACCGGTAGCAAGCCTGGTCCGTGAATGCCCGAAATGGGATGGATTACCGCTCACAGTGGATGTCAGCGCCACTTTCCCGGAAGGAGCCGCCGTACGGGATTATTACAGCCAGCAAATTGCGATAGTGAAGAACGGTCAAATAATGTTACAACCCGCTGCCACCAGCAACGGTTTACTCCTGCTGGAACGGGCAGAAACTGACACATCCGCCCCTTTCGACTGGCATAACGCCACGGTTTACTT  >  NC_000913/3737494‑3738074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑