Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,087,508	C→T	P268L (CCA→CTA)	metK →	methionine adenosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,087,508	0	C	T	100.0%	186.8 / NA	59	P268L (CCA→CTA)	metK	methionine adenosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/36);  total (23/36)

TTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACGTGAGTTCTTCGACCTGCGCCCATACGGTCTGATTCAGATGCTGGATCTGCTGCACCCGATCTACAAAGAAACCGCAGCATACGGTCACTTTGGTCG  >  NC_000913/3087216‑3087793                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                              tttCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCCCGGTGGCGGTGCATTCTCTGGTAAAGATCTATCaaaa                                                                                                                                                                                                                                                                                        >  2:655447/1‑300 (MQ=255)      gTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCAAGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGa                                                                                                                                                                                                                                                                                   >  1:399236/1‑300 (MQ=255)                   aaaaTCGTTGTTATCGATGCTTTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCACTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGTTCCTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAg                                                                                                                                                                                                                                                                        <  2:633350/298‑1 (MQ=255)                         gttgatgtCGATTCTGCCGTGCTGTACATTCAGAACTTTGAAGAGACCGACAAGCACTCGCTACAAGAAGTGGTGATGGAAGAGATCATCAAGCCAATCCTGCCCGCCGAATGGCTAACCGCTTCCACCAACTTCCTCATCAACCCGGCCGGTCGTTTCGTTATCGGTGGCCCACTGGTTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGGTACTTACGGCGGCGTGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACgc                                                                                                                                                                                                                                                                 <  2:637103/292‑1 (MQ=255)                         gTTGGTATCGATGTTGTCGGGCTTTCCACTCAGCGCTCTGAAGAGATCGACCAGAAAGCGCTGCAAGAAGTGGTAATGTAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTTGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACgc                                                                                                                                                                                                                                                                 <  2:247570/299‑1 (MQ=255)                         gTTGGTATCGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGGGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACgc                                                                                                                                                                                                                                                                 <  2:637093/299‑1 (MQ=255)                             gTATCGATGCTTTCGTGCTTTCCACTCAGCACTCTGAAGAGATTGACCAGAAATCGTTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCGATTCTGCCCGCTGAAGGGCTGACTTCTGCCACCAAGTTCTTNATCAATCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGTTCTGACTGATCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCAc                                                                                                                                                                                                                                                            <  2:221051/300‑1 (MQ=255)                                tCGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGtt                                                                                                                                                                                                                                                         <  1:91399/300‑1 (MQ=255)                                     gCTGTCGTGCTTTCCACTCAGCACTCCGAAGAAATCGACCAGAAATCGCGGCAAGAAGCGGTAAGGGAGGGGATCATCAAGTCAATTCTGCCCGCTGAATGGCTGATTGCGGCCACTAAATTCGTCATCAACCCGACCGGTCGTGTCGTTATCGTCGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAGATTATCGTTGATACCTACGACGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGt                                                                                                                                                                                                                                                     <  2:314872/299‑1 (MQ=255)                                      cTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCGCGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCATACGCAGCATGTTATGt                                                                                                                                                                                                                                                     >  2:544110/1‑298 (MQ=255)                                                  ccACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCCGCACGTTATGTCGCGAAAAACATc                                                                                                                                                                                                                                        >  2:398112/1‑299 (MQ=255)                                                    aCTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCGATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGtt                                                                                                                                                                                                                                     >  1:204615/1‑300 (MQ=255)                                                    aCTCAGCACTCTGAAGAGATCGAACAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGGTCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGTGTCACGGTGGCGGTGCATTCTATGGTAAAGATCTATCAAAAGTGGACCGTTCAGAAGCCTACGCAGCACGGTATGTAGCGAAAAACATTGtt                                                                                                                                                                                                                                     >  2:32175/1‑300 (MQ=255)                                                           aCTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCTTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTgctgct                                                                                                                                                                                                                               >  1:61058/1‑299 (MQ=255)                                                           aCTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTgctgct                                                                                                                                                                                                                               >  1:19758/1‑299 (MQ=255)                                                                gAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGctggcct                                                                                                                                                                                                                          >  1:654510/1‑299 (MQ=255)                                                                gAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTAGCGAAAAACATCGTTGCTGctggcct                                                                                                                                                                                                                          >  1:654812/1‑299 (MQ=255)                                                                   gagaTCGACTAGAAATCGCGACAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCTAATGGGTGACTGCGGTCTGACTGGTCGGAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCtggcctggcc                                                                                                                                                                                                                      <  2:27227/300‑1 (MQ=255)                                                                         gACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGt                                                                                                                                                                                                                <  1:283489/300‑1 (MQ=255)                                                                                 aTCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAAt                                                                                                                                                                                                        <  1:62319/300‑1 (MQ=255)                                                                                              gCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTAc                                                                                                                                                                                           >  1:12745/1‑300 (MQ=255)                                                                                                 gTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATTGTTGTGAAATTTCGGTTTCCTACGCa                                                                                                                                                                                        >  2:96705/1‑300 (MQ=255)                                                                                                                   aaGCCAATTCTGCCCGTTGAATGGCGGACCTCTGCCACCAAATTCCTCATCAACCCGACCGTTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCAGTCTGACTGGTCGGAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAAGTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCg                                                                                                                                                                      <  2:563631/300‑1 (MQ=255)                                                                                                                   aaGCCAATTCTGCCCGCTGAAGGGCTGACCTCTGCCACCAAATTCCTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGGAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCg                                                                                                                                                                      <  2:563606/300‑1 (MQ=255)                                                                                                                    aGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGATAAAGATCTATCACAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTAGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGtt                                                                                                                                                                                                 >  2:264835/1‑272 (MQ=255)                                                                                                                    aGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGAATGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGAGTGGCTGAACCGa                                                                                                                                                                     >  1:91978/1‑300 (MQ=255)                                                                                                                         aTTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTcc                                                                                                                                                                >  1:279201/1‑300 (MQ=255)                                                                                                                                  gCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATgg                                                                                                                                                         <  1:56273/298‑1 (MQ=255)                                                                                                                                   cTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACAGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTa                                                                                                                                                       >  1:647014/1‑299 (MQ=255)                                                                                                                                                ttCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTAc                                                                                                                                         <  1:640643/300‑1 (MQ=255)                                                                                                                                                       accaAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTtaga                                                                                                                                    <  1:632904/298‑1 (MQ=255)                                                                                                                                                       accaAATTCTTCATCAACCCAACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTgaga                                                                                                                                    <  2:661381/298‑1 (MQ=255)                                                                                                                                                                    tcaACCCGACCGTTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATANCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGaac                                                                                                                     <  1:494771/300‑1 (MQ=255)                                                                                                                                                                    tcaACCCGACCGTTCGTTTCATTATCAGTAGCCCAATGGGTGACTGTGGTCGGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGTTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTTTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTTAGAAAGTGCCTTCTGaac                                                                                                                     <  2:36963/300‑1 (MQ=255)                                                                                                                                                                               ggTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGAccc                                                                                                            <  1:379521/298‑1 (MQ=255)                                                                                                                                                                                gTCGTTTCGTTATAGGTGGCCCAATGTGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGTCTGCATGGCGCGTCACGGTTGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTAAccc                                                                                                            <  2:99950/297‑1 (MQ=255)                                                                                                                                                                                     ttcgttATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTa                                                                                                    <  1:641136/300‑1 (MQ=255)                                                                                                                                                                                     ttcgtgATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTggga                                                                                                    <  1:521632/300‑3 (MQ=255)                                                                                                                                                                                           atcgtcggCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACGTGAg                                                                                              <  2:255524/294‑1 (MQ=255)                                                                                                                                                                                                 tgCCCAATGTGTGACTGCGGTCTAACTGGTCGTAAAATTATCGTTGATACCGACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGTTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACGTGAg                                                                                              <  2:325683/293‑1 (MQ=255)                                                                                                                                                                                                   cccAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCCCGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGACTTCTGAACAACTGACCATGCTGATACGCGAGTTCTTCGa                                                                                      >  2:15188/1‑300 (MQ=255)                                                                                                                                                                                                   cccAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACg                                                                                                  >  2:94661/1‑288 (MQ=255)                                                                                                                                                                                                   cccAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACg                                                                                                  <  1:94661/288‑1 (MQ=255)                                                                                                                                                                                                              aCTGCGTTCTGACTATTCGTAAAATTATCGTGGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGTTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCGACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGaacaac                                                                                                                  <  2:62845/261‑1 (MQ=255)                                                                                                                                                                                                                tGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACGTGAGTTCTTCGACCTGCGCCCATAc                                                                         <  1:408743/300‑1 (MQ=255)                                                                                                                                                                                                                   ggTCTGGCTGTTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAANGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACGTGAGTTCTTCGACCTGCGCCCATACgg                                                                       <  2:524943/299‑1 (MQ=255)                                                                                                                                                                                                                    gttggACTGGTCGTAAAACTATCGTTGATACCTACGGCGGCATTGCGCGTCACGGTGGTGGTGCATTCTCTGGTAAAGATCTATCAAANGTGGACCGTTCCGCAATCTACGCAGCACGTTATGTCGCTAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGGTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTTAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGGACGTGAGTTCTTCGACCTGCGCCCATACgg                                                                       <  2:524936/294‑1 (MQ=255)                                                                                                                                                                                                                             gTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACGTGAGTTCTTCGACCTGCGCCCATACGGTCTGATTCa                                                              >  1:675689/1‑298 (MQ=255)                                                                                                                                                                                                                                                    tACGGCAGCATGGCGCGTCACGGTNGTGGTGCATTCTCGGTTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAACAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAGGTTCAGGTTTCCTACGCGATTGGCGTGGCTGAACCGACCTCCATCATGGTAGaagc                                                                                                                                                  <  2:205230/191‑3 (MQ=255)                                                                                                                                                                                                                                                                   cgTCCCGGTGGCGGTGCACTCTCTGGTAAAGATCTATCAAAAGTGGAACGTTCCGCAGCATACGCAGCACGTTATGTCGAGAAAAGCATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTACATTCTGAAAAACTGACCCTGTTGGTACGTGAGTTCTTCGAACTGTACCCATACGGTCTGATTCAGATACTGGATCTGCTGCACCCGATCTACAAAGaaa                           >  2:82621/1‑295 (MQ=255)                                                                                                                                                                                                                                                                     tCACGTTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGTACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGTCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCGGAACCGACCTCCATCATGGTAGAAGCTTTCGGTACTGAGAAAGTGCCTTCGGAACAACTGACCCTGCTGGTACg                                                                                                  <  2:276847/222‑1 (MQ=255)                                                                                                                                                                                                                                                                     tCACGGTGGCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACGTGAGTTCTTCGACCTGCGCCCATACGGTCTGATTCAGATGCTGGATCTGCTGCACCCGATCTACAAAGAAACCgcagc                    >  1:263857/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           tGGCGGTGCATTCTCTGTTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAGCATCGTTGCTACTGGCCTGGCCAATCGGTGTGAAATTCAGGTTTCCTGCGCAATCGGCGTGGCTGAACCAACCTCCATCATGGGAGAAACGTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACGTGAGTTCTTCGACCTGCGCCCATACGGTCTGATTCAGATGCTGGATCTGCTGCACCCGATCTACaaa                               <  2:26651/283‑1 (MQ=255)                                                                                                                                                                                                                                                                            ggCGGTGCATTCTTTGTTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTNGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGCCTGAACCGACCTCCATCATGTTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACGTGAGTTCTTCGACCTGCGCCCATACGGTCTGATTCAGATGCTGGATCTGCTGCACCCGATCTACAAAGAAACCGCAGCATACGGt             <  2:523982/300‑1 (MQ=255)                                                                                                                                                                                                                                                                             gCGGTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACGTGAGTTCTTCGACCTGCGCCCATACGGTCTGATTCAGATGCTGGATCTGCTGCACCCGATCTACAAAGAAACCGCAGCATACGGTc            >  1:90346/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                gTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACGTGAGTTCTTCGACCTGCGCCCATACGGTCTGATTCAGATGCTGGATCTGCTGCACCCGATCTACAAAGAAACCGCAGCATACGGt             <  2:346165/296‑1 (MQ=255)                                                                                                                                                                                                                                                                                gTGCATTCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGACCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACGTGAGTTCTTCGACCTGCGCCCATACGGTCTGATTCAGATGCTGGATCTGCTGCACCCGATCTACAAAGAAACCGCAGCATATGGTCACt         >  2:370019/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                     ttCTCTGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACGTGAGTTCTTCGACCTGCGCCCATACGGTCTGATTCAGATGCTGGATCTGCTGCACCCGATCTACAAAGAAACCGCAGCATACGGTCACTTTGGt    <  1:447424/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        tctGGTAAAGATCTATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACGTGAGTTCTTCGACCTGCGCCCATACGGTCTGATTCAGATGCTGGATCTGCTGCACCCGATCTACAAAGAAACCGCAGCATACGGTCACTTTGGTCg  <  2:590325/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                              TTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTATCGGTGGCCCAATGGGTGACTGCGGTCTGACTGGTCGTAAAATTATCGTTGATACCTACGGCGGCATGGCGCGTCACGGTGGCGGTGCATTCTCTGGTAAAGATCCATCAAAAGTGGACCGTTCCGCAGCCTACGCAGCACGTTATGTCGCGAAAAACATCGTTGCTGCTGGCCTGGCCGATCGTTGTGAAATTCAGGTTTCCTACGCAATCGGCGTGGCTGAACCGACCTCCATCATGGTAGAAACTTTCGGTACTGAGAAAGTGCCTTCTGAACAACTGACCCTGCTGGTACGTGAGTTCTTCGACCTGCGCCCATACGGTCTGATTCAGATGCTGGATCTGCTGCACCCGATCTACAAAGAAACCGCAGCATACGGTCACTTTGGTCG  >  NC_000913/3087216‑3087793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑