Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,104,887	G→A	S152N (AGC→AAC)	mltC →	membrane‑bound lytic murein transglycosylase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,104,887	0	G	A	100.0%	355.3 / NA	98	S152N (AGC→AAC)	mltC	membrane‑bound lytic murein transglycosylase C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (45/53);  total (45/53)

TTCAAAGAGGTGGTGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAGCAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTC  >  NC_000913/3104592‑3105170                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                            ttCAAAGAGGTGGTGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAAAGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCAGATTCGCTGGGAAGGTCGCGCAaacaac                                                                                                                                                                                                                                                                                         >  2:172650/1‑300 (MQ=255)       gagGTGGTGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTcgc                                                                                                                                                                                                                                                                                    >  2:719456/1‑299 (MQ=255)         ggtggtGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGGCGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGCTGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTcgc                                                                                                                                                                                                                                                                                    >  1:717563/1‑297 (MQ=255)          gtggtgATCGCTGATCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGACGGGGCAGAACCTGCCGCGTATTTGCGCCGGGCAATTATCAAAATGTTACTGATGGATGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGAAACCACCGGGCAGCCGATTCGATGGGACGGTCGCGCCAACACCTTCGCGGAt                                                                                                                                                                                                                                                                                >  2:158181/1‑300 (MQ=255)             gtgATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTAt                                                                                                                                                                                                                                                                             >  1:659820/1‑300 (MQ=255)               gATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATct                                                                                                                                                                                                                                                                           >  1:258670/1‑300 (MQ=255)               ctgcgagatgtgtataagagacaggAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATct                                                                                                                                                                                                                                                                           >  1:342374/25‑300 (MQ=255)                      ggTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCCAACAACTTCGCGGATTATCTGCTGaaa                                                                                                                                                                                                                                                                    >  1:494019/1‑300 (MQ=255)                      ggTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGaaa                                                                                                                                                                                                                                                                    >  1:5327/1‑300 (MQ=255)                      ggTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTCCGCGGATTATCTGCTGaaa                                                                                                                                                                                                                                                                    >  1:494072/1‑300 (MQ=255)                      ggTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAAACATCGCCGGGACAGAACCTGCCGCGCATTTGCGCAGGGCAATTATCAAAGCGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCCAACAACTTCGCGGATTATCTGCTGaaa                                                                                                                                                                                                                                                                    >  2:280254/1‑300 (MQ=255)                         ccTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATTTGCTGaaaaa                                                                                                                                                                                                                                                                  >  2:7867/1‑299 (MQ=255)                         ccTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTTCTGAAAAAc                                                                                                                                                                                                                                                                 >  1:455549/1‑300 (MQ=255)                         ccTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCAGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAAc                                                                                                                                                                                                                                                                 >  2:7812/1‑300 (MQ=255)                           tAAGGACTACGTTAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAAc                                                                                                                                                                                                                                                                 <  2:456237/298‑1 (MQ=255)                                   aCGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAaga                                                                                                                                                                                                                                                       >  1:470317/1‑300 (MQ=255)                                     gTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGANAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGCAAAACCGTCTGAAGAGc                                                                                                                                                                                                                                                     >  1:508175/1‑300 (MQ=255)                                       gAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGcc                                                                                                                                                                                                                                                    >  2:655355/1‑299 (MQ=255)                                              acCGATCAAGATCAGACCCGCAGCCGCATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCTGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGTTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCGTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCaa                                                                                                                                                                                                                                             <  2:58222/299‑1 (MQ=255)                                                  aTCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGc                                                                                                                                                                                                                                        <  1:166174/300‑1 (MQ=255)                                                     aaTATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCNGGGCAATTATCAAAACGTTATTGATGGTTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAAAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTg                                                                                                                                                                                                                                      <  2:568853/299‑1 (MQ=255)                                                     aaTATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCNGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTg                                                                                                                                                                                                                                      <  2:568713/299‑1 (MQ=255)                                                      atatCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGc                                                                                                                                                                                                                                     >  1:254307/1‑299 (MQ=255)                                                       tatCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATAGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCNATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGt                                                                                                                                                                                                                                   >  2:516746/1‑300 (MQ=255)                                                            gACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTatcat                                                                                                                                                                                                                              <  1:536713/300‑1 (MQ=255)                                                             aCCCGCAGCCACATCAGCTTCTATGACGGGAAGAGTATTCTCGAAACCATCGCCGGGACAGAGCTGGTCGCGTATTTGCGTTGGGCAAGGGTCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGCTGATGATATTACGATTTCGAAAGAACCTTTCCTTCGCGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCGAACAACTTCGCGAATTACCTGCTGAAAAACCGCCTGAAGAGCCGTAGCAACGTGCTGCGTatcatc                                                                                                                                                                                                                             <  2:412626/300‑1 (MQ=255)                                                             aCCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAAAGCCGCAGCAACGGGCTGCGTatcatc                                                                                                                                                                                                                             <  2:412627/300‑1 (MQ=255)                                                             aCCCGCAGCCACAGCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTatcatc                                                                                                                                                                                                                             <  2:412732/300‑1 (MQ=255)                                                                 gCAGCCACATCAACTTCGATGGCGGTACGATTGCTATCGAAACCATCGCCGGGACAGAACCTGCCGTGCATTGGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGTGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTAc                                                                                                                                                                                                                          <  2:580890/299‑1 (MQ=255)                                                                 gCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTAc                                                                                                                                                                                                                          <  1:130973/299‑1 (MQ=255)                                                                  cAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGNTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACa                                                                                                                                                                                                                         >  1:145402/1‑299 (MQ=255)                                                                   aGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAg                                                                                                                                                                                                                        <  1:666000/299‑1 (MQ=255)                                                                    gCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTNGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGc                                                                                                                                                                                                                       >  1:526040/1‑299 (MQ=255)                                                                             aCTTCGATGACGGTACGATTCCTATCGAAACCATCGACGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGCGACGATCCGAGTTCGGTCGATCCCTATTCCGACACTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACAGGGTAGCCGATTCGCTGGAACGGTCACGCACACAACTTCGCAGCTTATCTGCTGAAAAACAGTCTGAAGAGCCGCAGAAACGGGCTGCGTATCATCTCCAGCGtacccatcc                                                                                                                                                                                                             >  2:100605/1‑292 (MQ=255)                                                                             aCTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTAAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCa                                                                                                                                                                                                                   >  2:99831/1‑294 (MQ=255)                                                                               ttCGATGACGGTACGATTACTATCGAAACCGTCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATGATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAAc                                                                                                                                                                                                           <  2:657601/300‑1 (MQ=255)                                                                                tCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGTTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACa                                                                                                                                                                                                          <  2:609245/300‑1 (MQ=255)                                                                                   gtGACGGGAAGATCACTATCGAAACCACCGCCTGGACAGAACCTGCCGCTCATTTGCGCCGGGCAATCATCAAAATGTTATTGATTGGTGACGAGCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATACTACGATTTCTACAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTTAAAAACCGTCTGAATATCCGCAGCAACGGTCTTCGTATCATCtccctccccacccccccccccc                                                                                                                                                                                                       <  2:170335/299‑22 (MQ=255)                                                                                       cGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGcc                                                                                                                                                                                                   >  2:616610/1‑300 (MQ=255)                                                                                        ggTACGATTACTATCGAAACCATCGCCTGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGcc                                                                                                                                                                                                   <  1:348212/299‑1 (MQ=255)                                                                                           aCGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTTCGCCGTGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCTGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGTACAACACCGGGCAGCCGATTCGCTTGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAac                                                                                                                                                                                               <  2:305772/300‑1 (MQ=255)                                                                                           aCGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGNGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAac                                                                                                                                                                                               <  2:560612/300‑1 (MQ=255)                                                                                                    aTCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCATTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGAt                                                                                                                                                                                      >  1:55218/1‑300 (MQ=255)                                                                                                     tCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATa                                                                                                                                                                                     <  1:186387/300‑1 (MQ=255)                                                                                                         aaCCATCGCCTGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCNGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACg                                                                                                                                                                                 <  2:526040/300‑1 (MQ=255)                                                                                                         aaCCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACg                                                                                                                                                                                 <  1:364755/300‑1 (MQ=255)                                                                                                                    ggACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAat                                                                                                                                                                      >  1:718902/1‑300 (MQ=255)                                                                                                                        aGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCtctgtctcttatacacatctcgcag                                                                                                                                                                  <  1:423772/300‑25 (MQ=255)                                                                                                                                     cATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGc                                                                                                                                                     >  2:455276/1‑300 (MQ=255)                                                                                                                                     cATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGc                                                                                                                                                     >  1:666124/1‑300 (MQ=255)                                                                                                                                       tttGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCa                                                                                                                                                   <  1:420856/300‑1 (MQ=255)                                                                                                                                          gcgcCGGGCAATTATCAAGACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTTAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGc                                                                                                                                                <  1:497918/300‑1 (MQ=255)                                                                                                                                          gcgcCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGc                                                                                                                                                <  1:497932/300‑1 (MQ=255)                                                                                                                                                           aaaCGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTAGGCATGGTCCGCCAGGCGTCACGGAAATATGGc                                                                                                                                >  1:437643/1‑299 (MQ=255)                                                                                                                                                                 tATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTtgatg                                                                                                                         <  2:437043/300‑1 (MQ=255)                                                                                                                                                                  aTTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTAGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTtgatga                                                                                                                        >  2:708886/1‑300 (MQ=255)                                                                                                                                                                       tGGGTGACGATCTGAGTTTGGTCGATCTCTATTCCGACGGTGATGATATTGCGATTTCGAAAGAACCTTTCCTTTACGTTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGGGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAg                                                                                                                       <  2:289632/296‑1 (MQ=255)                                                                                                                                                                        gggTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTAGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCt                                                                                                                  >  1:419022/1‑300 (MQ=255)                                                                                                                                                                                    gAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAAt                                                                                                      >  2:664145/1‑300 (MQ=255)                                                                                                                                                                                              gATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGAc                                                                                             <  2:505111/299‑1 (MQ=255)                                                                                                                                                                                               aTCTCTATTCCTACTTGGATGATATTACGATTTCGAAAGGACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTTGTAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTTAAGAGCCGCAGCAACGTGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGAc                                                                                             <  2:131404/298‑1 (MQ=255)                                                                                                                                                                                                tctcTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTAGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGa                                                                                          >  2:62869/1‑300 (MQ=255)                                                                                                                                                                                                 ctctATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGaa                                                                                         >  1:655839/1‑300 (MQ=255)                                                                                                                                                                                                              ttGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGNAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAAcc                                                                            <  1:516746/300‑1 (MQ=255)                                                                                                                                                                                                                   gatATTACGATTTCTAAAGAAACTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTTTGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACTATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATACCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAg                                                                                                                       <  2:288983/252‑1 (MQ=255)                                                                                                                                                                                                                    atatTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGNGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATATCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTAt                                                                       >  2:515705/1‑299 (MQ=255)                                                                                                                                                                                                                    atatTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCAAGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTAt                                                                       >  1:289409/1‑299 (MQ=255)                                                                                                                                                                                                                                  aaaGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTTCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTcc                                                        <  2:455549/300‑1 (MQ=255)                                                                                                                                                                                                                                      aaCCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAAACACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCAGTTTCGAt                                                     >  2:89330/1‑299 (MQ=255)                                                                                                                                                                                                                                        cctttcctttACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCCGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTCTAACCCGTATGCGGTCAGCCGTTCCGAt                                                     >  2:327341/1‑297 (MQ=255)                                                                                                                                                                                                                                        cctttcctttACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATgc                                                   >  1:706145/1‑299 (MQ=255)                                                                                                                                                                                                                                             cctttACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCAGATGCGCt                                                >  2:287548/1‑297 (MQ=255)                                                                                                                                                                                                                                               tttACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGa                                            <  2:2539/299‑1 (MQ=255)                                                                                                                                                                                                                                                ttACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGAtt                                          <  1:719456/300‑1 (MQ=255)                                                                                                                                                                                                                                                     gTCAGTTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCACAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGc                                     <  2:40079/300‑1 (MQ=255)                                                                                                                                                                                                                                                     gTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGc                                     <  2:698582/300‑1 (MQ=255)                                                                                                                                                                                                                                                            ggtggACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCAAGGAAATATGGCGTTGGTGGGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCAGTTCCGATGCGCTGGGATTAATGCAggtggt                              >  2:307056/1‑300 (MQ=255)                                                                                                                                                                                                                                                              tggACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTac                            <  1:655355/300‑1 (MQ=255)                                                                                                                                                                                                                                                               ggACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTaca                           >  1:628220/1‑300 (MQ=255)                                                                                                                                                                                                                                                               ggACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCAAGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTaca                           >  2:275513/1‑300 (MQ=255)                                                                                                                                                                                                                                                                gACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCNCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTaca                           <  2:525255/299‑1 (MQ=255)                                                                                                                                                                                                                                                                 acaacaCCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTacaac                         <  1:715407/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 acaacaCCGGGCAGCCGATTCGCTGGGAAGGTCACGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGTAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTacaac                         <  2:258670/300‑1 (MQ=255)                                                                                                                                                                                                                                                                 acaacaCAGGGCAGCCGATTCGCTGGGAAGGTCACGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGTGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCNGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGATTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTacaac                         <  2:514851/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   aacaCCGGGCAGCCGATTCGCTGGGAAGTTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGGACTACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACAt                       <  2:681356/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   aacaCCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCNGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACAt                       <  1:158181/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   aacaCCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACAt                       <  1:79358/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   aacaCCGGGCAGCCGATTCGCGGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAAGATCGCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGAGGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTGCCGATGCGCTGGGAGTAATGCAGGTGGTACAACAt                       <  1:709222/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      acCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCATGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACt                    <  1:7867/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      acCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACt                    <  1:7812/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      acCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGCTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATAc                     <  2:659820/299‑1 (MQ=255)                                                                                                                                                                                                                                                                         gggCAGCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGc                  <  1:3304/299‑1 (MQ=255)                                                                                                                                                                                                                                                                              gCCGATTCGCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGaa            >  2:473191/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      gCTGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATgtgt    >  1:49003/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        ttggAAGTTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTTAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATAGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCATACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTc  <  2:55218/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                        tGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTc  <  1:361890/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        tGGGAAGGTCGCGCAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAAACACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTc  <  1:89330/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  gcgcAAACAACGTCGCGGAGTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACATCGCCACCATGAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGG‑AAAGATGTGTTc  <  2:95481/289‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   cgcAAACAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGTGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTAAGCCAGGCGTCAAGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCGATTCTGCAGACCGAATCTTCCTGTAACCCGTATGCGGTCAGCCGTTCCGa                                                      >  2:27130/1‑237 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                            TTCAAAGAGGTGGTGATCGCTGGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTCGATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGCCGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCCGACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAACACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAGCAACTTCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTC  >  NC_000913/3104592‑3105170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑