Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,065,449	G→A	L216L (TTG→TTA)	scpC →	propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,065,449	0	G	A	100.0%	288.7 / NA	83	L216L (TTG→TTA)	scpC	propionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (49/34);  total (49/34)

TTCTTCGGCGACATTGATGTTGCCGTCATTGAAGCATCGGCACTGGCACC‑GGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATC  >  NC_000913/3065153‑3065734                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                              ttcttcGGCGACATTGATGTTGCCGTCATTGAAGCATCGGCACTGGCACC‑GGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTAccc                                                                                                                                                                                                                                                                                            <  1:691308/300‑1 (MQ=255)           aCATTGATGTTGCCGTCATTGAAGCATCGGCACTGGCACC‑GGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGACCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCAGGTa                                                                                                                                                                                                                                                                                  >  2:118235/1‑300 (MQ=255)           aCATTGATGTTGCCGTCATTGAAGCATCGGCACTGGCACC‑GGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACCGGCGGATATTGTGATTCCTGGCGCGCCACCGCGACGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTa                                                                                                                                                                                                                                                                                  >  1:44485/1‑300 (MQ=255)             aTTGATGTTGCCGTCATTGAAGCATCGGCACTGGCACC‑GGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTaa                                                                                                                                                                                                                                                                                 <  1:554160/299‑1 (MQ=255)              ttGATGTTGCCTTCGTTGAAGCATCGGCACTGGCACC‑GGATGGTCGAGTCTGTTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCAGTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAata                                                                                                                                                                                                                                                                               <  2:592256/300‑1 (MQ=255)              ttGATGTTGCCGTCATTGAAGCATCGGCACTGGCACC‑GGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGANCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAata                                                                                                                                                                                                                                                                               <  2:236816/300‑1 (MQ=255)                gATGTTGCCGTCATTGAAGCATCGGCACTGGCACC‑GGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAatat                                                                                                                                                                                                                                                                              >  1:177775/1‑299 (MQ=255)                 aTGTTGCCGTCATTGAAGCATCGGCACTGGCACC‑GGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGCCCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCCCGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGc                                                                                                                                                                                                                                                                            >  1:469680/1‑300 (MQ=255)                 aTGTTGCCGTCATTGAAGCATCGGCACTGGCACC‑GGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGTCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCNCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTTTCGATCTTCCATGCAGTGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGc                                                                                                                                                                                                                                                                            <  2:231412/300‑1 (MQ=255)                 aTGTTGCCGTCATTGAAGCATCGGCACTGGCACC‑GGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGc                                                                                                                                                                                                                                                                            >  1:469688/1‑300 (MQ=255)                   gTTGCCGTCATTGAAGCATCGGCACTGGCACC‑GGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCNGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTg                                                                                                                                                                                                                                                                          <  1:147097/300‑1 (MQ=255)                         gTCATTGAAGCATCGGCACTGGCACC‑GGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATaa                                                                                                                                                                                                                                                                     >  1:709746/1‑299 (MQ=255)                                 aGCATCGGCACTGGCACC‑GGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATcc                                                                                                                                                                                                                                                            <  2:718877/300‑1 (MQ=255)                                  gCATCGGCACTGGCACCCGGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGACAACCAACCTTACCCGAAGCCGGTAatcgcgctggataggcgaaa                                                                                                                                                                                                                                                              >  1:243083/1‑282 (MQ=255)                                             ggCACC‑GGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCcagca                                                                                                                                                                                                                                                >  1:202813/1‑300 (MQ=255)                                              gCACC‑GGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCcagcag                                                                                                                                                                                                                                               <  1:448515/300‑1 (MQ=255)                                                         gTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATa                                                                                                                                                                                                                                     >  1:559505/1‑300 (MQ=255)                                                         gTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCANGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGCTCCGAAAAAGGTTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAACTCCACTGTGCCAGCAGATTGCCGATa                                                                                                                                                                                                                                     >  2:229806/1‑300 (MQ=255)                                                                  ggTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTc                                                                                                                                                                                                                             >  1:4444/1‑299 (MQ=255)                                                                  ggTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACATGGTCa                                                                                                                                                                                                                            >  1:4717/1‑300 (MQ=255)                                                                                  cGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGa                                                                                                                                                                                                            >  2:461097/1‑300 (MQ=255)                                                                                    gTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCNAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGaaa                                                                                                                                                                                                          >  2:562890/1‑300 (MQ=255)                                                                                           gccaaCCTGGTTGCTGCGGGCGAAGAAATTGATCATTGAACTCAATCACTATCATGATCCGCGCTTTGCAAAACTGGCGTATATTGTGATTCCTGGCGCTCCACCGCGGCGCAATAGCTCGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCTCTATGTGCAAATCGATCCGAAAAAAATTGTCGCCGTCCTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATACGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATgg                                                                                                                                                                                                       <  2:630239/292‑1 (MQ=255)                                                                                              gACCTGGCTGCTGCGGGCTAAGAAATTGATCATTGAACTCAATCACTATCACGATCCGCGCTTTGCAGAACTTGCGGATATTGTGATTCCTGGCGCGCCACCGCTGCGCAATAGTGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATTCTAAAAAGATTGTCGCCGTCGTGGA‑GACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCAt                                                                                                                                                                                                  <  2:632531/298‑1 (MQ=255)                                                                                              gACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTAGA‑AACCAAC‑TTACCCGACGCAGGTAATATGCTGGATAAGCAAAATCCAATGTGCAAGCAGATTGCCGATAACGTGGTCACGTTTTTATTGCAGGAAATGGCGCAt                                                                                                                                                                                                  >  2:65550/1‑298 (MQ=255)                                                                                                      tgctgcGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTc                                                                                                                                                                                        <  1:410004/300‑1 (MQ=255)                                                                                                                                gAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGt                                                                                                                                                              <  1:98940/300‑1 (MQ=255)                                                                                                                                       aTCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTgg                                                                                                                                                       <  1:662935/300‑1 (MQ=255)                                                                                                                                          aCTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGc                                                                                                                                                     <  2:434965/299‑1 (MQ=255)                                                                                                                                           cTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCaa                                                                                                                                                   >  1:423268/1‑300 (MQ=255)                                                                                                                                           cTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCaa                                                                                                                                                   >  2:680405/1‑300 (MQ=255)                                                                                                                                           cTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGAAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGt                                                                                                                                                         >  2:680885/1‑294 (MQ=255)                                                                                                                                             aTCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTACTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGAAat                                                                                                                                                  >  2:615881/1‑299 (MQ=255)                                                                                                                                                            ttGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCaa                                                                                                                                                   >  1:421254/1‑283 (MQ=255)                                                                                                                                                            ttGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTaa                                                                                                                                  >  1:450416/1‑300 (MQ=255)                                                                                                                                                            ttGCAGAACTGGCGGANATTGTGATTCCTGGCGCGCCACCGCTGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGNTGCAAAGTGGCGTGGGCANTATCAATAATGCGGTaa                                                                                                                                  >  1:598360/1‑300 (MQ=255)                                                                                                                                                                aGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGTTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATgg                                                                                                                               <  2:202813/299‑1 (MQ=255)                                                                                                                                                                 gAACTGGCGGATGTTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTGGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGCGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCAGGAATTTCTGCCGCTGCAAAGTGGCGTGGGTAACCTCAATAATGTGGTCATGgc                                                                                                                              >  2:478366/1‑299 (MQ=255)                                                                                                                                                                 gAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGgcg                                                                                                                             <  1:503879/300‑1 (MQ=255)                                                                                                                                                                          gATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCCAAGTGGCGTGGGc                                                                                                                                                     >  2:211550/1‑267 (MQ=255)                                                                                                                                                                                             cgcCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTTGGGGAAAACCCGGTAATtcctc                                                                                                 >  2:506457/1‑300 (MQ=255)                                                                                                                                                                                                       gcgcAATCGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGTAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCAGGTAATATGCTGGATAAGCAAAATCCAATGTGCCAGCAGATTGCCGATAAAGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAat                                                                                                                                                  >  2:209100/1‑241 (MQ=255)                                                                                                                                                                                                       gcgcAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTTGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTatgat                                                                                       <  2:452296/300‑1 (MQ=255)                                                                                                                                                                                                       gcgcAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAACTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTatgat                                                                                       <  1:399604/300‑1 (MQ=255)                                                                                                                                                                                                         gcAATAGCGTGTCGATTGTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGTAAATCCATCCGAAAGAGATTTTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGTAAATGGCGCGTGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGTTAATGGCGCGCCTGGGTGAAAACCCGGTAATCCCTCCttttcttccc                                                                                      <  2:389466/299‑7 (MQ=255)                                                                                                                                                                                                                 ttttCGATCTTCCATGCAATGGATCGCTTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGTAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGa                                                                              <  2:709746/296‑1 (MQ=255)                                                                                                                                                                                                                 ttgtCAATCTTCCAGGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATCGTCACCGTTGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCNAGTAGATTGCCGATAACGTGGTCACGTTCTTATTGCGGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTGTGATGTATTCGGa                                                                              <  2:220901/298‑1 (MQ=255)                                                                                                                                                                                                                 gtttcaaTCTTCCATGCAAGGGATCGCGTCGGTACCCGCTAGGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCNAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGa                                                                              <  2:221573/293‑1 (MQ=255)                                                                                                                                                                                                                 gtgtCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGTTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGa                                                                              <  1:291602/299‑1 (MQ=255)                                                                                                                                                                                                                  tttCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTANTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAg                                                                            <  2:526358/298‑1 (MQ=255)                                                                                                                                                                                                                    tCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAACTTCTGCCGCTGCAAAGTGGCGTGGGTAATATCAATAATGCGGt                                                                                                                                    >  2:51084/1‑242 (MQ=255)                                                                                                                                                                                                                           tCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATNTCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTCTGATGTATTCGGAAGTGCTACa                                                                     >  1:155279/1‑298 (MQ=255)                                                                                                                                                                                                                              aTGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCCATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTa                                                                                           >  2:483873/1‑273 (MQ=255)                                                                                                                                                                                                                              aTGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTCTGATGTATTCGGAAGTGCTACAGGAAt                                                                >  1:703787/1‑300 (MQ=255)                                                                                                                                                                                                                                gCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATc                                                               >  1:443617/1‑299 (MQ=255)                                                                                                                                                                                                                                     ggATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGtttcttatgtcttctga                                                                              >  2:478568/1‑265 (MQ=255)                                                                                                                                                                                                                                     ggATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGa                                                                              <  1:478568/279‑1 (MQ=255)                                                                                                                                                                                                                                      gATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCCATAATGCGGTAATGgc                                                                                                                              >  2:45935/1‑230 (MQ=255)                                                                                                                                                                                                                                           cgTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCCGGAAATGGCGCATGGGCGTATTCCGACGGAATTTCTGCCGCTGCAAAGt                                                                                                                                                              >  2:172311/1‑193 (MQ=255)                                                                                                                                                                                                                                           cgTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGt                                                                                                                                                              <  1:169106/193‑1 (MQ=255)                                                                                                                                                                                                                                           cgTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGt                                                                                                                                                              <  1:172311/193‑1 (MQ=255)                                                                                                                                                                                                                                           cgTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGt                                                                                                                                                              >  2:169106/1‑193 (MQ=255)                                                                                                                                                                                                                                           cgTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACCGGAATCGGTGGTGCAttt                                                   >  1:401762/1‑300 (MQ=255)                                                                                                                                                                                                                                           cgTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGAGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATTATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCAttt                                                   >  1:401998/1‑300 (MQ=255)                                                                                                                                                                                                                                            gccGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGCCGCCGTCGTGGA‑AACCAAC‑CTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGTGCATGTGCGTATTCCGCCGGAATTTCTGCCGCTGCAAATTGGCGTTGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAGACTCGGTAATTCCTCCGTTTATGATGTATTCGGAGGTGCTACAGGAATCggtggtacagtta                                                  <  2:44485/298‑8 (MQ=255)                                                                                                                                                                                                                                             tCGGTACCCGCTATGTGCAGATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGACAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGTTGCAAAGTGGCGTGGGCAATATCAATACTGCGGTAATGGCGCGTCTGTTGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTAc                                                 <  2:160552/300‑1 (MQ=255)                                                                                                                                                                                                                                                gTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTCTGATGTATTCGGAAGTGTTACAGGAATCGGTGGTGCATTTACt                                                >  1:229773/1‑298 (MQ=255)                                                                                                                                                                                                                                                gTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGACGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACt                                                >  1:355050/1‑298 (MQ=255)                                                                                                                                                                                                                                                      gCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGNGGGAAAACCCAGTAATTCCTCCGTTTCTGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAAcc                                         >  1:137103/1‑299 (MQ=255)                                                                                                                                                                                                                                                      gCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAAcc                                         >  1:668839/1‑299 (MQ=255)                                                                                                                                                                                                                                                      gCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAACAATGCGGTAATGGCGCGTCTGNGGGAAAACCCGGTAATTCCTCAGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTCCTGGAAAcc                                         >  1:136667/1‑299 (MQ=255)                                                                                                                                                                                                                                                           gtgCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCGTGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTCCAGGAATCGGTGGTGCATTTACTGGAAACCGGCaaa                                   >  1:469833/1‑300 (MQ=255)                                                                                                                                                                                                                                                           gtgCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCaa                                    >  1:626362/1‑299 (MQ=255)                                                                                                                                                                                                                                                           gtgCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACCGGAAACCGGCaaa                                   >  1:626373/1‑300 (MQ=255)                                                                                                                                                                                                                                                           gtgCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAAACCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGaaaa                                   >  1:470068/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    tCGCCTTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGcc          <  2:469688/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                    tCGCCGTCGTGTA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGcc          <  2:177775/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                    tCGCCGTCGTGGA‑AACTAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCAATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTTCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATGTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGcc          <  2:49651/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      gCCGTCGTGTA‑AACCAAC‑CTACCCGACGCCGGTAATATGCTGGATAAGCAAAAGCCCATGTGCCAGCAGATTGCCGATAACGTGTTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGcc          <  2:469680/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                       ccGTCGTGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGCAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTCTGATGTCTTCGGAAGTGCTACAAGAATCGGTGGTGCATTTACTGGAAACCTGCAAAATCAGCGGGGCCAGCGTCTCCAGCCTGa       >  2:629312/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         gtcgtGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAACAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACCGGAAACCGGCAAAATCAGCAGGGCCAGCGCATCCAGCCTGACa     >  2:656964/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           cgtGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCCGCGGGGCCAGCGCCTCCAGCCTGACAAt   >  1:673777/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            gtGGA‑AACCAAC‑TTACCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAAtc  <  2:668839/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                              TTCTTCGGCGACATTGATGTTGCCGTCATTGAAGCATCGGCACTGGCACC‑GGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGCTGCTGCGGGCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGA‑AACCAAC‑TTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATC  >  NC_000913/3065153‑3065734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑