Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,050,472	G→A	L66L (CTG→TTG)	gcvT ←	aminomethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,050,472	0	G	A	99.0%	328.2 / NA	96	L66L (CTG→TTG)	gcvT	aminomethyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (46/48);  total (47/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTC  >  NC_000913/3050177‑3050753                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                            tttgcttCATTAAACAGTTTTGCAGCTTTTGCTTGCGCATTCGGACCTTGCACCGCAATCATGGAAAGGTCAGCACGAACGGTAATTTCGATGCCTAGAGGTTCAGCGTGGTGGGTAATCCAGGAGAGGGCTTTTTCGCGGGTGGCGTAGTTAACAACGAGGCGNAAGAAATCTTCAGTAAAGGAGTATACGATGAANTCATCTATCACACCGCCATAGGCATTCAACATCCCCGAGTAAAGGGCTGTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGACAACGCAAAAAc                                                                                                                                                                                                                                                                                         <  2:544958/294‑1 (MQ=255)       tCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAANATCCCAGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCCGCAGATAACGCAAAAAC‑Tccc                                                                                                                                                                                                                                                                                    >  1:152887/1‑298 (MQ=255)       tCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑Tccc                                                                                                                                                                                                                                                                                    >  1:167439/1‑298 (MQ=255)       tCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCAGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑Tcca                                                                                                                                                                                                                                                                                    >  1:125118/1‑297 (MQ=255)       tCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCACGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑Tccc                                                                                                                                                                                                                                                                                    >  1:169430/1‑298 (MQ=255)       tCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGACAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑Tccc                                                                                                                                                                                                                                                                                    >  2:618503/1‑298 (MQ=255)                 gtgtGGCAGCTTTTGCNTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCNCCAGCAGATAACGCAAAANA‑TCCCGGGTGTGGCt                                                                                                                                                                                                                                                                          >  1:593772/1‑298 (MQ=255)                 gtgtGGCAGCTTTTGCCTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGc                                                                                                                                                                                                                                                                        >  1:61844/1‑300 (MQ=255)                         gCTTTTGCCTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TACCGGGTGCGGCTGCCGCGAAGa                                                                                                                                                                                                                                                                >  2:579927/1‑300 (MQ=255)                         gCTTTTGCCTGCGCACTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGACCGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTCATCCAGGAGAGGTCTTTTTCGCGTGTGACGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGGCGATGAGGTCATATATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCAGATTTTGGTGAGCTTCGCCACATCGTTCGACAGCAGATAACGCAAAAAC‑TCACAGGTGCGGCTGCCGCGAAGa                                                                                                                                                                                                                                                                >  2:336258/1‑300 (MQ=255)                                  tGCGCATTCGGCCCTTGCACTGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGTAGAGGTCTTTTTCGCGGGTGGCGGAGGTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATcgacga                                                                                                                                                                                                                                                         <  2:79932/298‑1 (MQ=255)                                           gtcccTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATTTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATAtg                                                                                                                                                                                                                                               <  2:666420/297‑1 (MQ=255)                                           ggCCCTTGCACGGCAATCATGTAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATAtgt                                                                                                                                                                                                                                              <  1:128000/300‑1 (MQ=255)                                           ggCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATAtgt                                                                                                                                                                                                                                              <  1:126446/300‑1 (MQ=255)                                            gCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGTAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTACCACACCGCCATAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATAtg                                                                                                                                                                                                                                               <  2:495970/298‑1 (MQ=255)                                             cccTTGCCCGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGa                                                                                                                                                                                                                                            >  1:423708/1‑300 (MQ=255)                                                       gCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAAc                                                                                                                                                                                                                                  <  2:666737/300‑1 (MQ=255)                                                          aTCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGCGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACAtt                                                                                                                                                                                                                               >  2:457799/1‑300 (MQ=255)                                                          aTCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACAtt                                                                                                                                                                                                                               >  1:536606/1‑300 (MQ=255)                                                             aTGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTcc                                                                                                                                                                                                                             >  1:405632/1‑299 (MQ=255)                                                                    ggTCATCACGAATGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGAAACATCAAACATTCCGGCATc                                                                                                                                                                                                                       >  2:358563/1‑298 (MQ=255)                                                                    ggTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGGAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGACAGAGGCATTCAACATCCAAGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAACAT‑TCCCGGGTGCGGCTGCAGCGAAGCTCGACGATGGTCATATGTGAAAAATCAAACATTCCGGCa                                                                                                                                                                                                                         >  2:358164/1‑296 (MQ=255)                                                                           aCGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGgtacgt                                                                                                                                                                                                                <  1:499445/298‑1 (MQ=255)                                                                             gAACGGTAACTTCGATGCCGAAAGGTTCAGCGTGGTGGGTAATCCATGAGAGGTCTTTTTCGCGGTTGTCGGAGTTAACAACGAGGCGTAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGATGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCg                                                                                                                                                                                                            <  2:194515/300‑1 (MQ=255)                                                                                 gttaacttCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCGGTAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCATTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCat                                                                                                                                                                                                         <  2:77200/293‑1 (MQ=255)                                                                                           aTGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCACGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTtcgtc                                                                                                                                                                                               >  1:283984/1‑299 (MQ=255)                                                                                                     gTTCAGCGTGTTGGGTAATCCAGGCGAGGTCTTTTTCGCGGGTGGCGGAGTTAACCACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCAAACCGCCAGAGGCATTCAACATCCCCGAGTCAAGGGCTTTGCCGCTTTTGGTGAGCTTAGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGACGATCGACGATGGTAATATGTGAAAAATCAAACATTCCAGCATCGATACGCACCGCATGATGTTCGCCGATTTGTGAAc                                                                                                                                                                                    >  2:248967/1‑300 (MQ=255)                                                                                                     attCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAAc                                                                                                                                                                                    >  2:688124/2‑300 (MQ=255)                                                                                                             tgtTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGc                                                                                                                                                                            <  1:579927/300‑1 (MQ=255)                                                                                                               ttGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAg                                                                                                                                                                          <  1:459831/300‑1 (MQ=255)                                                                                                                  ggTAATCCAGTAGAGGTNTTTTTCGCGGGTGGCGGANTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCNAACCGTAATGCAGCgg                                                                                                                                                                       <  1:609537/300‑1 (MQ=255)                                                                                                                  ggTAATCCAGGAGAGGTNTTTTTCGCGGGTGGCGGANTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCNAACCGTAATGCAGCgg                                                                                                                                                                       <  1:609526/300‑1 (MQ=255)                                                                                                                      aTCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTCTCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGcatc                                                                                                                                                                   >  1:392908/1‑300 (MQ=255)                                                                                                                      aTCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGcatc                                                                                                                                                                   >  2:685350/1‑300 (MQ=255)                                                                                                                      aTCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGcatc                                                                                                                                                                   >  1:5716/1‑300 (MQ=255)                                                                                                                          attagagGTCTTTTTCGCGGGTGGCGGAGTTAACAACTAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑GCCCGGGTACGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCatcatc                                                                                                                                                                <  2:180863/296‑1 (MQ=255)                                                                                                                          agtagagGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCATAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCatcatc                                                                                                                                                                <  2:622976/296‑1 (MQ=255)                                                                                                                           gtagagGTCTTTGTCGCTGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAATTAGTAGACGATTAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACTTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCatcatc                                                                                                                                                                <  2:178926/296‑1 (MQ=255)                                                                                                                               agGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCATAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATANCGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCg                                                                                                                                                          <  2:515318/300‑1 (MQ=255)                                                                                                                                    tttttCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGa                                                                                                                                                      <  1:126612/299‑1 (MQ=255)                                                                                                                                       ttCGCGGGTGGCGGAGTTAACAACGAGGCGTAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCATAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATc                                                                                                                                                  <  1:92290/300‑1 (MQ=255)                                                                                                                                       ttCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCATAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATc                                                                                                                                                  <  1:91038/300‑1 (MQ=255)                                                                                                                                                      gTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAATCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAgcgac                                                                                                                                   >  2:37441/1‑298 (MQ=255)                                                                                                                                                      gTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAAGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGcgcc                                                                                                                                   >  1:61941/1‑300 (MQ=255)                                                                                                                                                        tAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGcgccgc                                                                                                                                 <  1:222465/300‑1 (MQ=255)                                                                                                                                                                     gaagaaATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCACTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTTACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCTGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGtgttg                                                                                                                    <  2:59687/300‑1 (MQ=255)                                                                                                                                                                      aagaaATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGTCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATAGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGTCGCAAAGCGTGtgttg                                                                                                                    <  2:271373/299‑1 (MQ=255)                                                                                                                                                                                 aggaaaGGAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAgg                                                                                                       <  1:537099/297‑1 (MQ=255)                                                                                                                                                                                 aGTAAAGTAGTATACGATGAGGGCATCTATCACACCGTCATATGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGGTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATCCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATACAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGTAAAGTGTGTGTTGTTCGTACA‑AAgg                                                                                                       <  2:67009/300‑1 (MQ=255)                                                                                                                                                                                 aGTAAAGTAGTAGACGATTAGTTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAgg                                                                                                       <  2:454789/300‑1 (MQ=255)                                                                                                                                                                                 aGTAAAGTAGTAGACGATTAGGTCATCTATCACACCGCCATATGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAgg                                                                                                       <  2:316927/300‑1 (MQ=255)                                                                                                                                                                                 aGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAgg                                                                                                       <  1:536939/300‑1 (MQ=255)                                                                                                                                                                                 aGTAAAGTAGTAGACGATGAGGTCATCTACCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAgg                                                                                                       <  1:395917/300‑1 (MQ=255)                                                                                                                                                                                       gtagtaGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCACGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGTGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACACCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCt                                                                                                  >  2:67476/1‑299 (MQ=255)                                                                                                                                                                                             gACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGc                                                                                           <  1:457799/300‑1 (MQ=255)                                                                                                                                                                                               cGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACNCAAAAAC‑TCCCGGGTGCGGATGCCGCGAAGATNGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCa                                                                                         >  2:533630/1‑300 (MQ=255)                                                                                                                                                                                                  tGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCt                                                                                      <  1:477434/300‑1 (MQ=255)                                                                                                                                                                                                   gAGGTCATCTATCACACCGCCAGCGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCACTTTTGGTGAGCTTAGCAACATCGCTCGCCAGCAGATAACACAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCAGTGGAGATCAAACATGTGAGTGCAGGAAAGAGTGTGTTGTTCGTACACAAGG‑GTCTGTTGTGCCATCtt                                                                                     >  2:58896/1‑300 (MQ=255)                                                                                                                                                                                                   gAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCACGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGAAACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGAGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTACCATCtt                                                                                     >  2:58376/1‑300 (MQ=255)                                                                                                                                                                                                   gAGGTCATCTATCACACCGCCAACGGCATTCCCCATCCCCGAGTAAAGGGCTTTGCCGCTTTCGGCAAGCTTCGACACACCATTCGCCAGCAGATAACGCA‑AAACATCCCGGGTGCGGCTGCCGCGAAGATCGCCGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACAGTCATGCAGCGGCATTATCCAGCCGTGGAAATCCAACATGAGAGTGCCGCaaa                                                                                                                              >  2:58744/1‑260 (MQ=255)                                                                                                                                                                                                       tCATCTATCACACCGCCATAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTcc                                                                                 <  1:3891/300‑1 (MQ=255)                                                                                                                                                                                                       tCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGTTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTcc                                                                                 <  1:2358/300‑1 (MQ=255)                                                                                                                                                                                                       tCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTcc                                                                                 <  1:455936/300‑1 (MQ=255)                                                                                                                                                                                                         aTCTATCACACCGCCATAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCACCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTc                                                                               <  2:55977/300‑1 (MQ=255)                                                                                                                                                                                                         aTCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACC‑AAGGAGTCTGTTGTGCCATCTTGTCCTc                                                                               >  1:585610/1‑300 (MQ=255)                                                                                                                                                                                                         aTCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTc                                                                               >  1:88428/1‑300 (MQ=255)                                                                                                                                                                                                         aTCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCAGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTc                                                                               >  1:213306/1‑300 (MQ=255)                                                                                                                                                                                                         aTCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCACCGGTACGTACCACATGACGTTCGTCGATTTGCGAACCATAATGCAGCGGCATCATCAAGCCGTAGAAATCAACCATGCGAGCGCCGCAAAGAGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCCTCTTGTcc                                                                                 >  2:199529/1‑298 (MQ=255)                                                                                                                                                                                                          tCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTCa                                                                              >  1:488993/1‑300 (MQ=255)                                                                                                                                                                                                           cTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAGGGGCTTTGCAGCTTTTGGTGAGCTTCGCCACATCGTCCGCAAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTACCGCGAAGATCGCCGATGGTCATATGTGACACATTAAACATTCCGGCATCGGTACGTACCGCCTGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCAGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGGTGTTCGTACA‑AAAGAGTCTGTTGTGCCAtattatactcat                                                                             >  2:135712/1‑289 (MQ=255)                                                                                                                                                                                                           cTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAT‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTAAGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTCAt                                                                             >  2:103257/1‑300 (MQ=255)                                                                                                                                                                                                           cTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACCTCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCCCCATGCGAGCACCGCAAAGCGTGTGTTGTTCGTACA‑AAGGGGTCTGTTGTGCCATCTTGTCCTCAt                                                                             >  2:135701/1‑300 (MQ=255)                                                                                                                                                                                                                   ccGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCACGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAAt                                                                        >  2:18929/1‑297 (MQ=255)                                                                                                                                                                                                                    cGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTTGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGaa                                                                                                                                                     >  2:43920/1‑220 (MQ=255)                                                                                                                                                                                                                    cGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGaa                                                                                                                                                     <  1:43920/220‑1 (MQ=255)                                                                                                                                                                                                                     gCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACCCATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGGACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCg                                                                   <  2:577687/300‑1 (MQ=255)                                                                                                                                                                                                                     gCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCg                                                                   <  1:721551/300‑1 (MQ=255)                                                                                                                                                                                                                          agGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAAC‑TCCCGGGTGCTGCTGCCGCGAAGATCGACGATGTTCATGTGTTACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCgtgtgggg                                                                                                                    <  2:271970/247‑4 (MQ=255)                                                                                                                                                                                                                               ttCAACATCCTCCAGGAAAGGGCCTTGCCGCTTTTGGGGAGCTGCGCCACATCGTTCGCCAGCAGATAACGCAAGAAC‑TCCCGGGTGCAGCTGCCGCGCAGATTTACGATGGTCAGAGGTTACACATCAAACATTCCTACATCGGTACGTACCACATTATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCTTGGAAATCCACCATGCTAGCGTCGCAAAACGTGTGGTGTTCGTACA‑AAGGAGTCTGGTGTGCCATCTTGTCCCTGTGGAAGAAGCGGGGCTGACaa                                                         <  2:339077/300‑1 (MQ=255)                                                                                                                                                                                                                                                  gggCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTa                                      >  1:476205/1‑300 (MQ=255)                                                                                                                                                                                                                                                  gggCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGAAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTTACAACTTTTTCATGGTGAAATTa                                      >  1:477070/1‑300 (MQ=255)                                                                                                                                                                                                                                                      tttGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGAATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATcac                                  <  1:89295/300‑1 (MQ=255)                                                                                                                                                                                                                                                               tttGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAAcc                         <  1:688124/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          tCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCACAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTCTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGa               >  2:429075/1‑299 (MQ=255)                                                                                                                                                                                                                                                                          tCGCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGATCATATGTGACACATCAAACATTCCGGTATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGCAACCCACCCTGCGAGCGCCCCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCAATCTTGTCATCATTGAATAAGCGGGGCTGACAACTTTTTAATGGTGAAATTATCACCAAGAAACACAGCATCGGa               >  2:428985/1‑299 (MQ=255)                                                                                                                                                                                                                                                                            gCCACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCAATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTCCATGGTGAAATTATCAACACGAAACCCAGCATCGGAGcc            >  2:357372/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             ccccaTCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCAGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCAAGCCGCGGAAATCCACCCTGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTCCTTGAATCAGCGGGGCTGACAACTTTTTCATGGTGAAATTCTcacacagaagacaagcataggagcaa           >  2:89602/4‑277 (MQ=255)                                                                                                                                                                                                                                                                             ccACCTCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGCACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTCGTTCGTACA‑AAGGAGTCTGTTGTGCAATCTTGTCCTCATTGAACAAGCGGGGCTGACAACTTTTTAATGGTGAAATTATcac                                  >  2:89539/1‑277 (MQ=255)                                                                                                                                                                                                                                                                             ccACATCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCa           >  2:90959/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               acaTCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACt         >  1:718531/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               acaTCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCACATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCAc          <  2:295031/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                 aTCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTc        <  2:476205/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                  tCGTTCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCTGCTGCCGCGAAGATCGACGATGGTCATATTTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTc        <  2:477070/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                  tCGTTCGCCAGCAGATAACACAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTGGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGTTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTc        <  2:258751/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                      tCGCCAGCAGATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTc  <  2:593772/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               gATAACGCAAAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGATGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTc  <  2:423708/291‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                            TGGGCGTCATTAAACAGTGTGGCAGCTTTTGCCTGCGCATTCGGCCCTTGCACGGCAATCATGGAAAGGTCATCACGAACGGTAATTTCGATGCCGAAAGGTTCAGCGTGTTGGGTAATCCAGGAGAGGTCTTTTTCGCGGGTGGCGGAGTTAACAACGAGGCGGAAGAAATCTTCAGTAAAGTAGTAGACGATGAGGTCATCTATCACACCGCCAGAGGCATTCAACATCCCCGAGTAAAGGGCTTTGCCGCTTTTGGTGAGCTTCGCCACATCGTTCGCCAGCAGATAACGCAGAAAC‑TCCCGGGTGCGGCTGCCGCGAAGATCGACGATGGTCATATGTGACACATCAAACATTCCGGCATCGGTACGTACCGCATGATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACA‑AAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTC  >  NC_000913/3050177‑3050753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑