Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,875,647	T→C	D228G (GAC→GGC)	cysD ←	sulfate adenylyltransferase subunit 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,875,647	0	T	C	97.8%	331.1 / ‑2.2	91	D228G (GAC→GGC)	cysD	sulfate adenylyltransferase subunit 2
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (40/49);  total (41/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

ATCATCCAGGCTTCGACGCCGCCTTCATTGGCGATTTGTTGTGCAAGTGCGGTGTTCATCTTAAAAATACCCCTGACGTTTTTTCAGCTCCATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGTC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGATCCCAGCGATGGAAGCGGTCACGGAAAGAGTAAATTCGCTCTTTAGCGCGGGATTTCTCTTCGTCACGGCGCGCACCACCGAAGGC  >  NC_000913/2875361‑2875939                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                              atcatcCAGGCTTCGACGCCGCCTTCATTGGCGATTTGTTGTGCAAGTGCGGTGTTCATCTTAAAAATACCCCTGACGTTTTTTCAGCTCCATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAatcatcat                                                                                                                                                                                                                                                                                                 >  1:118932/1‑300 (MQ=255)    atcCAGGCTTCGACGCCGCCTTCATTGGCGATTTGTTGTGCAAGTGCGGTGTTCATCTTAAAAATACCCCTGACGTTTTTTCAGCTCCATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCaa                                                                                                                                                                                                                                                                                              >  1:556259/1‑300 (MQ=255)        aGGCTTCGACGCCGCCTTCATTGGCGATTTGTTGTGCAAGTGCGGTGTTCATCTTAAAAATACCCCTGACGTTTTTTCAGCTCCATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACAt                                                                                                                                                                                                                                                                                           <  1:683069/299‑1 (MQ=255)         ggCTTCGACGCCGCCTTCATTGGCGATTTGTTGTGCAAGTGCGGTGTTCATCTTAAAAATACCCCTGACGTTTTTTCAGCTCCATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATAc                                                                                                                                                                                                                                                                                         >  1:50462/1‑300 (MQ=255)           cTTCGACGCCGCCTTCATTGGCGATTTGTTGTGCAAGTGCGGTGTTCATCTTAAAAATACCCCTGACGTTTTTTCAGCTCCATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATAcc                                                                                                                                                                                                                                                                                        >  2:286336/1‑299 (MQ=255)                         tCATTGGCGATTTGTTGTGCAAGTGCGGTGTTCATCTTAAAAATACCCCTGACGTTTTTTCAGCTCCATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAAc                                                                                                                                                                                                                                                                         <  1:45524/300‑1 (MQ=255)                         tCATTGGCGATTTGTTGTGCAAGTGCGGTGTTCATCTTAAAAATACCCCTGACGTTTTTTCAGCGCCATAGACCCCGCCTGTTCGCGGTCAATCACGCGGCCCTGACGTTCACTGTTGGTGTAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTATTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑AGCAAGCATCATCAACATATCGTCGCGTTCCAGAAc                                                                                                                                                                                                                                                                         <  1:45532/300‑1 (MQ=255)                           agcggcaaTTTGTTGTGCAAGTGCGGTGTTCATCTTAAAAACACCCCTAACATTTTTTCAGCTCCATAGACCCCTCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCACCTCTTCGATGATTGCCGGCAGGGTTTGTGCATTGGACGCCACCGCACCGGTCAGCGGCCAGCAG‑‑CTCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑atcaatca                                                                                                                                                                                                                                                                                                     <  2:211081/263‑1 (MQ=255)                                                   ggTGTTCATCTTAAAAATACCCCTGACGTTTTTTCAGCTCCATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGc                                                                                                                                                                                                                                                <  1:226133/299‑1 (MQ=255)                                                    gtgtTTATCTTAAAAATACCCCTGACGTTTTTTCAGCTCCATAGACCCCGCCTGGTCGCGGTCAATCACGCTGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCgg                                                                                                                                                                                                                                              <  2:284045/300‑1 (MQ=255)                                                    gtgtTCATCTTAAAAATACCCCTGACGTTTTTTCAGCTCCATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCgg                                                                                                                                                                                                                                              <  1:450755/300‑1 (MQ=255)                                                    gtgtTCATCTTAAAAATACCCCTGACGCTTTTTCAGCTCCATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGTTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCgg                                                                                                                                                                                                                                              <  2:145128/300‑1 (MQ=255)                                                       ttCATCTTAAAAATACCCCTGACGTTTTTTCAGCTCCATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAAc                                                                                                                                                                                                                                           <  1:41561/300‑1 (MQ=255)                                                       ttCATCTTAAAAATACCCCTGACGTTTTTTCAGCTCCATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCATACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAAc                                                                                                                                                                                                                                           <  1:42329/300‑1 (MQ=255)                                                       ttCATCTTAAAAATACCCCTGACGTTTTTTCAGCGCCATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTCGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGTTCGATACGGTTGCC‑ATCAATCATCATCAACGTACCGTCGCGTTCCAGAACCAGACGTTCCGCAGCGAGATATAGCGGAAc                                                                                                                                                                                                                                           <  2:424216/300‑1 (MQ=255)                                                          aTCTTAAAAATACCCCTGACGTTTTTTCAGCTCCATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACaa                                                                                                                                                                                                                                         <  2:494214/299‑1 (MQ=255)                                                             ttAAAAATACCCCTGAAGGTTTTTCAGCTCCATAGACCCCGCCTGTTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGTTCGATACGGTTGCC‑ATCAATCATCATCAAAATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGt                                                                                                                                                                                                                                      <  2:138518/299‑1 (MQ=255)                                                                                tttttCAGCTCCATAGACCCCGCCTGGTCGCAGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATg                                                                                                                                                                                                                  <  2:176887/300‑1 (MQ=255)                                                                                   ttCAGCTCCATAGACCCCGCCGGGTCGCGGTCAATCACGCGGTCCTGACGTTCACTGTTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGTAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGTAAACGCACCATCCGTTTTTTAATCACTTCGCCCGTTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTac                                                                                                                                                                                                               <  2:369446/300‑2 (MQ=255)                                                                                   ttCAGCGCCATAGACCCCGCCTGTTCGCGGTCAATCACGCGGCCCTAACGTTCACTGGGGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACGCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAAGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACTGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTGTCCAGCCAGATggat                                                                                                                                                                                                               <  2:369625/300‑4 (MQ=255)                                                                                       gCTCCATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑Gcc                                                                                                                                                                                                          >  1:21617/1‑300 (MQ=255)                                                                                       gCTCCATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTAGATATTTTCCAGCCAGATGTATT‑Gcc                                                                                                                                                                                                          >  1:22048/1‑300 (MQ=255)                                                                                       gCTCCATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCAGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑Gcc                                                                                                                                                                                                          >  1:22922/1‑300 (MQ=255)                                                                                       gCTCCATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCAAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑gac                                                                                                                                                                                                          >  1:22953/1‑298 (MQ=255)                                                                                       gCTCCATAGACCCCGCCTAGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑Gcc                                                                                                                                                                                                          >  1:21699/1‑300 (MQ=255)                                                                                         tCCATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCa                                                                                                                                                                                                         >  1:695970/1‑299 (MQ=255)                                                                                          ccATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTCATCCCTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGGGGTATGGCGGAACAATGCCGat                                                                                                                                                                                                                                  >  2:16124/1‑274 (MQ=255)                                                                                          ccATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGCCGTTCCGCAGCGAGGTATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCAAGa                                                                                                                                                                                                       >  2:244101/1‑300 (MQ=255)                                                                                               gACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGTTGGAAAC‑CAGCATCTCTTCGATGATTTCCTGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACNTCGCCCAGTTGCAGGTCGATACGGTTGCC‑ATCNATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCTGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATcc                                                                                                                                                                                                  <  2:534666/300‑1 (MQ=255)                                                                                                    cGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑Ct                                                                                                                                                                                             >  2:655495/1‑299 (MQ=255)                                                                                                     gCCTGGTCGCGGTCGATCACGCGGCCCTGACGTTCACTGTTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCNCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCANCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCTAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTc                                                                                                                                                                                            <  2:550794/299‑1 (MQ=255)                                                                                                     gCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTc                                                                                                                                                                                            <  1:387204/299‑1 (MQ=255)                                                                                                        tGGTCGCGGTCAATCACGCGGCCCTGATGTTCACTGGTGGTGGAAAT‑CGGCATCTCTTCGATGATTTCCGTCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCATCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGataccc                                                                                                                                                                                                  <  2:166457/291‑4 (MQ=255)                                                                                                         ggTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTcc                                                                                                                                                                                       <  1:664239/300‑1 (MQ=255)                                                                                                          gTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCa                                                                                                                                                                                      >  1:36508/1‑300 (MQ=255)                                                                                                             gcgGTCAATCACGCGGCCCTGACGTTCACTGTTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCTTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGtt                                                                                                                                                                                   <  1:256964/300‑1 (MQ=255)                                                                                                               ggTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTa                                                                                                                                                                                  <  1:94809/299‑1 (MQ=255)                                                                                                                gTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTaga                                                                                                                                                                                >  1:674060/1‑300 (MQ=255)                                                                                                                gTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTA‑CTCGGTCCAGTTaga                                                                                                                                                                                >  1:674068/1‑300 (MQ=255)                                                                                                                 tCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTagag                                                                                                                                                                               <  2:676795/300‑1 (MQ=255)                                                                                                                  cAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGCACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTTGGTCCAGTTagaga                                                                                                                                                                              >  2:137232/1‑300 (MQ=255)                                                                                                                   aatcctgtggCCCTGANCTTCACTGGTGGTGGAAAN‑CAGCATCCCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTGNCTCGGTCCAGTTagag                                                                                                                                                                               <  1:599589/291‑1 (MQ=255)                                                                                                                   aaccgcgatgCCCTGACNATCACTGGTGGGGGAAACNCAGCATTGCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGTAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTGNCTCGGTCCAGTTagag                                                                                                                                                                               <  1:599311/291‑1 (MQ=255)                                                                                                                   aaaagcgtggCCCTAACNGTCACTGGGGGTGGAAACNCAGCATCGCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGTAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTGNCTCGGTCCAGTTagag                                                                                                                                                                               <  1:599302/292‑1 (MQ=255)                                                                                                                   aaTCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTagagag                                                                                                                                                                             <  1:233510/300‑1 (MQ=255)                                                                                                                           ggCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCAGCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCATCTAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGa                                                                                                                                                                     <  1:401929/300‑1 (MQ=255)                                                                                                                            gCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGAc                                                                                                                                                                    >  2:259923/1‑300 (MQ=255)                                                                                                                            gCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTT‑TCTATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCgg                                                                                                                                                                                                                                              <  2:418606/228‑1 (MQ=255)                                                                                                                            gCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTT‑TCTATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCgg                                                                                                                                                                                                                                              >  1:418606/1‑228 (MQ=255)                                                                                                                            gCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGCTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGAc                                                                                                                                                                    >  1:63226/1‑300 (MQ=255)                                                                                                                               cTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCAAGTTAGAGAGCGGGAAGa                                                                                                                                                                     >  1:475070/1‑296 (MQ=255)                                                                                                                                            gtgttggAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGTTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGcc                                                                                                                                                     <  2:356066/295‑1 (MQ=255)                                                                                                                                             tggtggAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCtt                                                                                                                                                   <  1:648/300‑1 (MQ=255)                                                                                                                                                    aaC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAt                                                                                                                                            >  1:647576/1‑300 (MQ=255)                                                                                                                                                    aaC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAAt                                                                                                                                            >  2:584911/1‑300 (MQ=255)                                                                                                                                                          gcctctcTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGc                                                                                                                                        >  1:101740/4‑299 (MQ=255)                                                                                                                                                          gCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGcc                                                                                                                                       >  2:447256/1‑300 (MQ=255)                                                                                                                                                                     atgatTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCGGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCTAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑tg                                                                                                                        <  2:575256/300‑1 (MQ=255)                                                                                                                                                                      tgatTTCCGGCATTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑tg                                                                                                                        <  2:427829/299‑1 (MQ=255)                                                                                                                                                                                 aGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCTTTTTTTAATCACTTCGCCNGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCTAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTc                                                                                                             <  2:553892/299‑1 (MQ=255)                                                                                                                                                                                 aGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTc                                                                                                             <  2:176148/299‑1 (MQ=255)                                                                                                                                                                                 aGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGGTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTc                                                                                                             <  2:401596/299‑1 (MQ=255)                                                                                                                                                                                  gtgtTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTcc                                                                                                            >  2:607294/1‑299 (MQ=255)                                                                                                                                                                                          gCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGa                                                                                                 >  1:428167/1‑300 (MQ=255)                                                                                                                                                                                                gACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCACGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTc                                                                                           >  2:19637/1‑300 (MQ=255)                                                                                                                                                                                                    ccACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGAt                                                                                       >  1:94850/1‑300 (MQ=255)                                                                                                                                                                                                      accgcaccgGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCCTCATCAACATACCGTCGAGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGATc                                                                                      >  2:155179/1‑299 (MQ=255)                                                                                                                                                                                                         gcaccgGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGATCCCa                                                                                   >  1:485533/1‑299 (MQ=255)                                                                                                                                                                                                         gcaccgGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCAAGCAAGATGTATT‑GACAGATATCCTG‑CTCGGTACAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCATTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCAGG‑‑GCGCTGATTTTTCGGATCCCa                                                                                   >  2:83333/1‑299 (MQ=255)                                                                                                                                                                                                              ggTCAGCGGCCAGCAGGGCCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATTCGCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTGGGTCGTCCTGTGCCACAGCTCCGGGAGCGCTGATTTTTccggac                                                                                      >  1:241197/1‑295 (MQ=255)                                                                                                                                                                                                              ggTCAGCGGCCAGCAG‑‑CCCAGCGTACGTAAACGCACCAGCCGTTTTTTAATCACTTCGCCCGGTTGCAGTTCGAGACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGATc                                                                                      <  2:32075/291‑1 (MQ=255)                                                                                                                                                                                                              ggTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGATCCCAGCGAt                                                                              >  1:263583/1‑299 (MQ=255)                                                                                                                                                                                                                  aGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGNCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCANTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGATCCCAGCGATGGAAg                                                                         <  2:547444/300‑1 (MQ=255)                                                                                                                                                                                                                             g‑‑cCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAAAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGTCACAGCTCCGG‑‑GCGCTGATTTTTCGGATCCCAGCGATGGAAGCGGTCGCGGaa                                                              >  2:5852/1‑300 (MQ=255)                                                                                                                                                                                                                                    gCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGATCCCAGCGATGGAAGCGGTCACGGAAAGAGt                                                         >  1:216766/1‑300 (MQ=255)                                                                                                                                                                                                                                    gCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGAAGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGATCCCAGCGATGGAAGCGGTCACGGAAAGAGt                                                         >  1:216948/1‑300 (MQ=255)                                                                                                                                                                                                                                           gAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGATCCCAGCGATGGAAGCGGTCACGGAAAGAGTAAATTc                                                   >  2:635047/1‑299 (MQ=255)                                                                                                                                                                                                                                                      tCCGTTTTTTAATCACTTCGCCCTGTTGCAGTTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGTACGTTNCGCAGCTAGATATAGCGGAACAATTTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGATCCCAGCGATGGAAGCGGTCACGGAAAGAGTAAATTCGCTCTTTAgcgc                                       <  2:556259/300‑1 (MQ=255)                                                                                                                                                                                                                                                      tCCGTTTTTTAATCACTTCGCCCGGTTGCAGTTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGATCCCAGCGATGGAAGCGGTCACGGAAAGAGTAAATTCGCTCTTTAgcgc                                       <  1:483200/300‑1 (MQ=255)                                                                                                                                                                                                                                                      tCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGATCCCAGCGATGGAAGCGGTCACGGAAAGAGTAAATTCGCTCTTTAgcgc                                       <  1:483188/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         gCCCGGTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCAACAGCTACGG‑‑GCGCTGCTTTTTCGGATCCCAGCGATGGAAGCGGTCACGGAAAGAGTAAATTCGCTCTTTAGCGCGGGATTTCTATTCGTCAc                     >  2:263775/1‑299 (MQ=255)                                                                                                                                                                                                                                                                              gTTGCAGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGATCCCAGCGATGGAAGCGGTCACGGAAAGAGTAAATTCGCTCTTTAGCGCGGGATTTCTCTTCGTCACGgcgc                >  1:473558/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                   aGGTCGATACTGTTGCC‑ATCAATCATCATCAACATACTGTCGCATTCCATAACCGGACGTTCCGCAGCTAGATATAGCGGAACGATGTCGGTATTTTCCAGACAGATGTATT‑GCCAAATATCCTG‑CGTGGTCCAGTTAGAGAGCGGGAGGACGCGGATGCGTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGGTTTTTCGGATCCCAGCGATGGAAGCGGTCACGGAAAGAGTAAATTCGCTCTTTAGCGCTGGATTTCTCTGCGCCACGGCGCAcacc           <  2:478442/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                   aGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGTACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGATCCCAGCGATGGAAGCGGTCACGGAAAGAGTAAATTCGCTCTTTAGCGCGGGATTTCTCTTCGTCACGGCGCGcacc           <  2:21617/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                   aGGTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCTAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTT‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGATCCCAGCGATGGAAGCGGTCACGGAAAGAGTAAATTCGCTCTTTAGCGCGGGATTTCTCTTCGTCACGGCGCGcacc           <  2:478709/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                   aGGTCGATACGGTTGCC‑ATCAATCATCATAAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATTTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGATCCCAGCGATGGAAGCGGTCACGGAAAGAGTAAATTCGCTCTTTAGCGCGGGATTTCTCTTCGTCACGGCGCGcacc           <  2:22922/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                   aGGTCGATACATTTGCC‑ATCAATCATCAGCAACATACCGCCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATGTAGCGGAACAATGTCAATATTTGCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAGGACGCGGATGCTGTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAACTCCGG‑‑GCGCTGATTTTTCGGATCCCAGCGATGGAAGCGGTCACGGAAAGAGTGAATTCGCTCTTTAGCGCGGGATTTCTCTTCGTCACGGCGCGcacc           <  2:22953/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                    ggTCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGANCCCAGCGATGGAAGCGGTCACGGAAAGAGTAAATTCGCTCTTTAGCGCGGGATTTCTCTTCGTCACGGCGCGcaccac         >  1:518519/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      tCGATACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATAGAGCGGAACAAGGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCATTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGATCCCAGCGATGGAAGCGGTCACGGAAAGAGTAAATTCGCTCTTTAGCGCGGGATTTCTCTTCGTCACGGCGCGcacc           <  2:21699/296‑1 (MQ=255)                                                                                                                                                                                                                                                                                         aTACGGTTGCC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGATCCCAGCGATGGAAGCGGTCACGGAAAGAGTAAATTCGCTCTTTAGCGCGGGATTTCTCTTCGTCACGGCGCGCACCACCGAAg    <  1:469751/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                           aCGGTTGCC‑ATCAATCATCAGCAACATACCGTCGCGTTCCAGGACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCATCCAGATGTATT‑GCCAGATACCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGATCCCAGCGATGGAAGCGGTCACGGAAAGAGTAAATTCGCTCTTTAGCGCGGGATTTCTCTTCGTCACGGCGCGCACCACCGAAggc  <  2:118932/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                              ATCATCCAGGCTTCGACGCCGCCTTCATTGGCGATTTGTTGTGCAAGTGCGGTGTTCATCTTAAAAATACCCCTGACGTTTTTTCAGCTCCATAGACCCCGCCTGGTCGCGGTCAATCACGCGGCCCTGACGTTCACTGGTGGTGGAAAC‑CAGCATCTCTTCGATGATTTCCGGCAGTGTTTGTGCATTTGACTCCACCGCACCGGTCAGCGGCCAGCAG‑‑CCCAGCGTACGGAAACGCACCATCCGTTTTTTAATCACTTCGCCCGGTTGCAGGTCGATACGGTTGTC‑ATCAATCATCATCAACATACCGTCGCGTTCCAGAACCGGACGTTCCGCAGCGAGATATAGCGGAACAATGTCGATATTTTCCAGCCAGATGTATT‑GCCAGATATCCTG‑CTCGGTCCAGTTAGAGAGCGGGAAGACGCGGATGCTTTCGCCTTTGTTAATTTGCCCGTTG‑‑TAGT‑‑TGTGCCACAGCTCCGG‑‑GCGCTGATTTTTCGGATCCCAGCGATGGAAGCGGTCACGGAAAGAGTAAATTCGCTCTTTAGCGCGGGATTTCTCTTCGTCACGGCGCGCACCACCGAAGGC  >  NC_000913/2875361‑2875939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑