Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,795,848	G→A	V59M (GTG→ATG)	glaR →	DNA‑binding transcriptional repressor GlaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,795,848	0	G	A	100.0%	208.1 / NA	62	V59M (GTG→ATG)	glaR	DNA‑binding transcriptional repressor GlaR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/24);  total (38/24)

GGATTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAAAGCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGGTGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTT  >  NC_000913/2795571‑2796137                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                  ggATTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAAAGCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGt                                                                                                                                                                                                                                                                             <  1:398190/300‑1 (MQ=255)     tATCTGTACCGTGCCAATTATGGCTCGCTGGAAAAAGCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGATCAGGAGTCACATCATGACCATTACGTATATGGATGACTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGCCATCGCGTTATGCACTTGGCGTTGGGCAGTTACGGGAAGCTCTTTCGAAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGc                                                                                                                                                                                                                                                                          >  2:148963/1‑299 (MQ=255)     tATCTGTACCGTGCCAATTATGGCTCGCTGGAAAAAGCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCACAAAGGGTATCGGGTGGc                                                                                                                                                                                                                                                                          >  1:206852/1‑299 (MQ=255)     tATCTGTACCGTGCCAATTATGGCTCGCTGGAAAAAGCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGc                                                                                                                                                                                                                                                                          >  1:338753/1‑299 (MQ=255)     tATCTGTACCGTGCCAATTATGGCTCGCTGGAAAAAGCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAAATGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGc                                                                                                                                                                                                                                                                          >  1:161627/1‑299 (MQ=255)       tCTGTACCGTGCCAATTATGGCTCGCTGGAAAAAGCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATNATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTc                                                                                                                                                                                                                                                                       <  1:525199/300‑1 (MQ=255)               gTGCCAATTATGGCTCGCTGGAAAAAGCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTc                                                                                                                                                                                                                                                                 <  1:108241/298‑1 (MQ=255)                 gCCAATTATGGCTCGCTGGAAAAAGCGGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGATCAGGAGTCACACCATGACCATTACTTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGTTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCagag                                                                                                                                                                                                                                                             <  1:503374/300‑1 (MQ=255)                    aaTTATGGCTCGCTGGAAAAAGCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGATCAGGAGTCACACCATGACCATTACGTCTATGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCa                                                                                                                                                                                                                                                           >  1:267643/1‑299 (MQ=255)                                  ggAAAAAGCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGATCAGGAGTCACACCATGACCATTACGTCTATGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGatat                                                                                                                                                                                                                                            >  1:253475/1‑300 (MQ=255)                                                            acacCCGTTCATAATCCGCACTAATCATGATCAGGAGCCACACTATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAg                                                                                                                                                                                                                  <  2:26300/300‑1 (MQ=255)                                                                       taataCGCGCTGATCATGATTAGGAGTCACACCATTACCATTACGTCTCTGGGTGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTTACATCGCGTTATGCACTTGGCGTTGGGCTGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTg                                                                                                                                                                                                       <  2:423236/300‑1 (MQ=255)                                                                       taataCGCGCTAACCATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACTGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGTAAGCGATGTTAGTg                                                                                                                                                                                                       <  2:31359/300‑1 (MQ=255)                                                                           aCGCGCTGATCATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTc                                                                                                                                                                                                   <  1:695559/300‑1 (MQ=255)                                                                             gcgcTGATCATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAANAATTACGAATGAGTTTGCTGACATCGCGTTATNCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGCGTCt                                                                                                                                                                                                  >  2:549979/1‑299 (MQ=255)                                                                              cgcTGATCATGATCAGGAGTCACACCATGACCATTACGTCTATGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCt                                                                                                                                                                                                  >  1:207013/1‑298 (MQ=255)                                                                                  gatcatgatcaGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGNAACTGGTGGCGGAACGGCTGGTCAAGGTGATGAATCAAAAAGGGTATCGGGTGGCGTATATGTCAGAGCAGGAGCTGCTCGATATTTTCGAAGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGAt                                                                                                                                                                                            >  2:237016/1‑300 (MQ=255)                                                                                  gatcatgatcaGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGAt                                                                                                                                                                                            >  2:711793/1‑300 (MQ=255)                                                                                        gatcaGGAGTCACACCGTGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGccc                                                                                                                                                                                       >  2:259252/1‑299 (MQ=255)                                                                                        gatcaGGAGTCACACCATGACCATTACGTCTATGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGATGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGccc                                                                                                                                                                                       >  1:93632/1‑299 (MQ=255)                                                                                             ggAGTCACACCATGACCATTACGTCTATGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGt                                                                                                                                                                                  >  2:485494/1‑299 (MQ=255)                                                                                              gAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGCAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCACCTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCGGGAGCTGCTCGATATTTTCGACGCACGTGCCGATATGGAAGCGCTGTTAGTGAGTCTGGCGATTGCCCGCggc                                                                                                                                                                                  >  2:151547/1‑297 (MQ=255)                                                                                              gAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGt                                                                                                                                                                                  >  2:448016/1‑298 (MQ=255)                                                                                              gAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGt                                                                                                                                                                                  >  1:655457/1‑298 (MQ=255)                                                                                              gAGTCACACCATGACCATTACGTCTATGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTNCTGACATCGCGTTATGCACTTGGCGTTGGGCCNTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGt                                                                                                                                                                                  >  2:540501/1‑298 (MQ=255)                                                                                              gAGTCACACCATGACCATTACGTCTATGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGNAACGGCTGGTAACGGTGATGAATCAAAAAGGGTATAGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGt                                                                                                                                                                                  >  2:223593/1‑298 (MQ=255)                                                                                                    cacCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGa                                                                                                                                                                          >  1:422483/1‑300 (MQ=255)                                                                                                    cacCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCACGCGGTGGCGATGa                                                                                                                                                                          >  2:21864/1‑300 (MQ=255)                                                                                                          gACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGa                                                                                                                                                                    >  2:676917/1‑300 (MQ=255)                                                                                                            ccATTACGTCTATGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTAACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGa                                                                                                                                                                    >  2:187344/1‑298 (MQ=255)                                                                                                               ttACGTCTCTGGATGGCGAGCGCTTGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGGAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGTGCCGTTACTGGAAGCTCTGTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGc                                                                                                                                                                 <  2:180114/298‑1 (MQ=255)                                                                                                                   gTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACg                                                                                                                                                            <  1:187344/299‑1 (MQ=255)                                                                                                                         ggATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGc                                                                                                                                                     >  1:640529/1‑300 (MQ=255)                                                                                                                                       gactgAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATACACTTGGCGTTGGGCCGTTACTGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACATTCTCGCAAAAgc                                                                                                                                               <  2:69538/290‑1 (MQ=255)                                                                                                                                            aagaaCGATATTATTCGCGGTAATTTTCAACCGGATGAAAAACTACGAATGAGTTTGCCGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGTTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATctgctg                                                                                                                                     <  1:671946/297‑1 (MQ=255)                                                                                                                                                   atatTATTCGCGGTAATTTTCAACCGGATGAAAAATCACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAGAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGCGTAAGCtt                                                                                                                            >  2:262794/1‑299 (MQ=255)                                                                                                                                                      ttattCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATAGGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAgg                                                                                                                        <  2:207013/300‑1 (MQ=255)                                                                                                                                                               gTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGAc                                                                                                                >  2:695831/1‑299 (MQ=255)                                                                                                                                                                 aaTTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGcc                                                                                                             <  1:200992/300‑1 (MQ=255)                                                                                                                                                                                                 tttGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTTAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCg                                                                             <  2:636806/300‑1 (MQ=255)                                                                                                                                                                                                      tGACATCGCGTTATGCACTTTGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGtcatc                                                                        <  2:655457/300‑1 (MQ=255)                                                                                                                                                                                                      tGACATCACGTTATGCACTTGGCGTGGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGGATCAAAAAGGGTATTGGGTGGCGGCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGtcatc                                                                        <  2:311862/300‑1 (MQ=255)                                                                                                                                                                                                       gACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGtcatc                                                                        <  2:311908/299‑1 (MQ=255)                                                                                                                                                                                                             gcgTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGtt                                                                 >  1:497376/1‑300 (MQ=255)                                                                                                                                                                                                               gTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGttt                                                                >  2:600028/1‑299 (MQ=255)                                                                                                                                                                                                               gTTATGCACTTGGCGTNGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAANTGCTTGATGAGTGGGATCNGCGTCATCAGGCGTTTc                                                               >  1:605391/1‑300 (MQ=255)                                                                                                                                                                                                                           gCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGCCGCCAGCGAGAAAATGCTTGATGAGTGGGATTTGCGTCATAAGGTGTTTCCTAcagaaatt                                                    >  2:15586/1‑292 (MQ=255)                                                                                                                                                                                                                             gTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATtgt                                                  <  1:183457/299‑1 (MQ=255)                                                                                                                                                                                                                                 ggCCGTTACGGTAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCgg                                             <  1:645224/300‑1 (MQ=255)                                                                                                                                                                                                                                     gTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGc                                           <  1:237016/298‑1 (MQ=255)                                                                                                                                                                                                                                     gTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCt                                          >  2:58092/1‑299 (MQ=255)                                                                                                                                                                                                                                     attacagaaaactctTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAATGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGtcgtc                                                                        >  2:241352/12‑266 (MQ=255)                                                                                                                                                                                                                                        aCGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCggg                                            >  1:586521/1‑294 (MQ=255)                                                                                                                                                                                                                                                ctctTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGAAGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACt                              >  2:172196/1‑300 (MQ=255)                                                                                                                                                                                                                                                   tttCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTAtt                           >  2:628784/1‑300 (MQ=255)                                                                                                                                                                                                                                                   tttCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTAt                            <  1:682555/299‑1 (MQ=255)                                                                                                                                                                                                                                                     tCGCAACTGGTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTAttt                          >  1:167889/1‑299 (MQ=255)                                                                                                                                                                                                                                                         aaCTGTTGGCGGAACGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTtgctg                      <  1:58092/299‑1 (MQ=255)                                                                                                                                                                                                                                                                     aaCGGCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGNCGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAAc         <  1:148963/300‑1 (MQ=255)                                                                                                                                                                                                                                                                         gCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGNTCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGTTGCAAATGCGTGAACGgtt     >  1:149225/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         gCTGGTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGNTCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGgtt     >  1:149214/1‑300 (MQ=255)                                                                                                                                                                                                                                                                            ggTCACGGTGATGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGgttgtt  >  1:219175/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                  GGATTATCTGTACCGTGCCAATTATGGCTCGCTGGAAAAAGCTGGTATTGTGGCAAAAAACACCCGTTCATAATACGCGCTGATCATGATCAGGAGTCACACCATGACCATTACGTCTCTGGATGGCTATCGCTGGCTGAAGAACGATATTATTCGCGGTAATTTTCAACCGGATGAAAAATTACGAATGAGTTTGCTGACATCGCGTTATGCACTTGGCGTTGGGCCGTTACGGGAAGCTCTTTCGCAACTGGTGGCGGAACGGCTGGTCACGGTGGTGAATCAAAAAGGGTATCGGGTGGCGTCTATGTCAGAGCAGGAGCTGCTCGATATTTTCGACGCCCGCGCCAATATGGAAGCGATGTTAGTGAGTCTGGCGATTGCCCGCGGTGGCGATGAGTGGGAGGCAGACGTTCTCGCAAAAGCGCATCTGCTGAGTAAGCTTGAGGCCTGTGACGCCAGCGAGAAAATGCTTGATGAGTGGGATCTGCGTCATCAGGCGTTTCATACGGCAATTGTGGCGGGCTGTGGTTCTCACTATTTGCTGCAAATGCGTGAACGGTTGTT  >  NC_000913/2795571‑2796137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑