Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,587,112	C→T	A461V (GCA→GTA)	narQ →	sensor histidine kinase NarQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,587,112	0	C	T	100.0%	245.2 / NA	75	A461V (GCA→GTA)	narQ	sensor histidine kinase NarQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/38);  total (37/38)

GCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGGCACGTTAGTGAGTATTAGCTTTCGCTCTGCGGA  >  NC_000913/2586817‑2587406                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                       gCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTg                                                                                                                                                                                                                                                                                                    <  2:179539/300‑1 (MQ=255)   gACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGa                                                                                                                                                                                                                                                                                                  <  2:439191/300‑1 (MQ=255)    aCCATCGCCCGCGAATTGCACGACTCGCTGGCTCCGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGCCTACTTTCCGCCTGACGCTGCAGCAGGCAGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATCCGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGAt                                                                                                                                                                                                                                                                                                 >  2:259947/1‑300 (MQ=255)    aCCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGTGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTCCAAAATCCAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGAt                                                                                                                                                                                                                                                                                                 >  2:259889/1‑300 (MQ=255)    aCCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCATGGTACTGGAt                                                                                                                                                                                                                                                                                                 >  2:611732/1‑300 (MQ=255)              gcgAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGc                                                                                                                                                                                                                                                                                       <  1:46924/300‑1 (MQ=255)                gAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTTGATGCGCAAATGCa                                                                                                                                                                                                                                                                                      >  1:686155/1‑299 (MQ=255)                     gCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCa                                                                                                                                                                                                                                                                                >  1:410240/1‑300 (MQ=255)                       aCGACTCGCTTGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGTAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCAt                                                                                                                                                                                                                                                                               <  2:434241/299‑1 (MQ=255)                                     cAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGTAGTTACGCGAGTTGNTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCATCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCCGGTGCATGTGTTGCAAactatt                                                                                                                                                                                                                                                                >  2:530711/1‑300 (MQ=255)                                           cTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGaa                                                                                                                                                                                                                                                          >  1:77737/1‑300 (MQ=255)                                           cTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAAACCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGaa                                                                                                                                                                                                                                                          >  1:228170/1‑300 (MQ=255)                                                  acttacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGc                                                                                                                                                                                                                                                   <  1:657106/300‑1 (MQ=255)                                                  aattacttagccattggcctgtccggacgCGTTCGATACCGTACGAGAACGAAAACGCACAAAGTATAACGGCCGCTGTTTCCCAGGAGTTGAATGATGATTATAGGCAGTTAGGCGAGCCGTGGTATATTTTCTGTGTGAAACGGCGGCAGGCGGATCTTTCATCCGCATCTGATC‑TAATGCCGAATGTACTACAAAACCAACCCAGCGCCAAACTGACCCTCG‑CTCGTCGACTGCCAGCCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGc                                                                                                                                                                                                                                                   <  1:308168/272‑1 (MQ=255)                                                     tacGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGa                                                                                                                                                                                                                                                >  1:620394/1‑300 (MQ=255)                                                        gTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGaa                                                                                                                                                                                                                                               >  2:599337/1‑298 (MQ=255)                                                                    cGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCac                                                                                                                                                                                                                                  >  1:85336/1‑299 (MQ=255)                                                                      ttACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCacgcc                                                                                                                                                                                                                               >  2:470951/1‑300 (MQ=255)                                                                              gCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCATGGTACTGGATGTGCAAATGCAGGTGCATTTGTTGCAGATTATTCGCGAAGCGGTGCTGAATGCGATGAAACaagcaaacgcca                                                                                                                                                                                                                        >  2:345598/1‑299 (MQ=255)                                                                                 ttCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGa                                                                                                                                                                                                                    >  1:478055/1‑300 (MQ=255)                                                                                             aGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCa                                                                                                                                                                                                         >  1:579264/1‑299 (MQ=255)                                                                                                     ccACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGCTGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAATGCCAGCGAAATCGCCGTCAGtt                                                                                                                                                                                                      >  2:52296/1‑294 (MQ=255)                                                                                                          gCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCAGTCAGTTGCGTCACcgc                                                                                                                                                                                            >  2:100450/1‑299 (MQ=255)                                                                                                               aaGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCCCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGa                                                                                                                                                                                      >  1:375013/1‑300 (MQ=255)                                                                                                               aaGTATCATGGCCGATTTTTCCAAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGa                                                                                                                                                                                      >  1:375419/1‑300 (MQ=255)                                                                                                               aaGTATCATGGCCGATTTTTCCAAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCATGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGa                                                                                                                                                                                      >  1:374993/1‑300 (MQ=255)                                                                                                                 gTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCCACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGAc                                                                                                                                                                                     >  2:139029/1‑299 (MQ=255)                                                                                                                                      atgcggtGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATc                                                                                                                                                                <  1:362663/293‑1 (MQ=255)                                                                                                                                      atgCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATc                                                                                                                                                                <  2:620394/297‑1 (MQ=255)                                                                                                                                      atgCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATc                                                                                                                                                                <  1:389731/297‑1 (MQ=255)                                                                                                                                      atgCGTTGAATGATGCTTATCGGCAGTTACGCGAACTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACACCAACACCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATc                                                                                                                                                                <  2:65279/297‑1 (MQ=255)                                                                                                                                      aGGCGTTTAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATc                                                                                                                                                                <  2:179796/299‑1 (MQ=255)                                                                                                                                      aGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATc                                                                                                                                                                <  1:657977/299‑1 (MQ=255)                                                                                                                                        gCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCNATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGt                                                                                                                                                             >  1:507951/1‑300 (MQ=255)                                                                                                                                        aggTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCg                                                                                                                                                              <  1:411310/297‑1 (MQ=255)                                                                                                                                          gTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCgct                                                                                                                                                            >  2:411310/1‑297 (MQ=255)                                                                                                                                                           ggCAGTTACGAGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGATGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTTCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATc                                                                                                                                           <  2:717081/299‑1 (MQ=255)                                                                                                                                                              aGTTACGCGAGCTGTTTACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGTAT‑TGAGGTAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCAGCTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTg                                                                                                                                       <  2:495490/300‑1 (MQ=255)                                                                                                                                                              aGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGATGTAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTTACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCANTTGTTGCAAATTATTCGCGAAGCGGTGCTGAANGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGt                                                                                                                                        <  2:531783/299‑1 (MQ=255)                                                                                                                                                               gTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGATGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGGTGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAGTCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATc                                                                                                                                                                <  2:665949/274‑1 (MQ=255)                                                                                                                                                               gTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATc                                                                                                                                                                >  1:665949/1‑274 (MQ=255)                                                                                                                                                                 tACGCTAGCTGTTGATTACTTTCCGCCTGACGCTGCAGCATGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTTCGCCAACCCTGGTACTGTATGCGCAAATGCAGGTGCGTTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCATTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCTTGATAACGGGATTGGTATCGTTGAAc                                                                                                                                    <  2:54232/300‑1 (MQ=255)                                                                                                                                                                       ggCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGTCGGATCTCCCCTCCGCAT‑TTAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAAc                                                                                                                                    <  2:54253/293‑1 (MQ=255)                                                                                                                                                                        gCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGATGGAAATGCTGGATACGTTACAAGATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCTTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCTAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCATTTGCGTCACCGCGCCGGACGGCAAGCACACGGTTTATATc                                                                                                                                                                <  2:93615/265‑1 (MQ=255)                                                                                                                                                                        gCTGTTGACTACTTGCTGCCTGACGCTGCAGCATGCGGATCTCCCCTCCGCAT‑TGATGGAAATGCTTGATACGTTACAAAATCAAACCAGCGCCAGACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCTCTAAGCGGTGCTGAATGCGATGAAGCACGCCAACTCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATc                                                                                                                                                                <  2:179882/265‑1 (MQ=255)                                                                                                                                                                           gTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAAcc                                                                                                                          >  2:434085/1‑300 (MQ=255)                                                                                                                                                                           gTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAAcc                                                                                                                          >  1:75839/1‑300 (MQ=255)                                                                                                                                                                               ggtaatttCCGCCTGACGCTGCAGCAGGTGGATCTCCCCTCCGCAT‑TGAGGGCAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTAGACT‑GTCGTCTGCCAACCCTGGTACTGGATGCGCAAATGTAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCGAGCGAAAt                                                                                                                                                                                                                 >  2:132232/6‑209 (MQ=255)                                                                                                                                                                                         gCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTAt                                                                                                             <  1:584867/299‑1 (MQ=255)                                                                                                                                                                                           tgaccgtgaagaaggccGGATCTCCCCTCCGCAT‑TCAGGGAAACGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATgg                                                                                                           <  1:246185/283‑1 (MQ=255)                                                                                                                                                                                            tGACGCTGCAGCATGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATgg                                                                                                           <  2:693905/298‑1 (MQ=255)                                                                                                                                                                                            tGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTc                                                                                                         <  1:77473/300‑1 (MQ=255)                                                                                                                                                                                             gACGCTGCAGCAGGCAGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCATCGCCAAACCTACCCTCGACT‑GCCGTCTGTCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTACAAATTATTCGCGAAGCGTTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTc                                                                                                         <  2:67616/299‑1 (MQ=255)                                                                                                                                                                                                 cTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAat                                                                                                    >  1:182765/1‑300 (MQ=255)                                                                                                                                                                                                             gATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTNTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGaa                                                                                        >  1:520135/1‑300 (MQ=255)                                                                                                                                                                                                                    ccTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAAcgcgc                                                                                   >  2:500825/1‑298 (MQ=255)                                                                                                                                                                                                                              ttaGGGAAATGCTGGATGCGTTACAAAAGCAAACCAGCGCCAAGCTTACCCTCGACT‑GCCTTCTGCCAACCCTGGTACTGGATNCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGag                                                                               <  2:567883/291‑2 (MQ=255)                                                                                                                                                                                                                                aGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGNATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGt                                                                      <  1:523402/300‑1 (MQ=255)                                                                                                                                                                                                                                 gggAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGTATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTg                                                                     <  1:683188/300‑1 (MQ=255)                                                                                                                                                                                                                                  ggAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGNCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTgg                                                                    <  2:507951/300‑1 (MQ=255)                                                                                                                                                                                                                                    aaaTGCTGGATACGTTACAAAATCAAGCCAGCGCCAAACTGACCCTCGGCT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATTAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTg                                                                     <  2:85104/297‑1 (MQ=255)                                                                                                                                                                                                                                     aaTGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGAc                                                                 <  1:505163/300‑1 (MQ=255)                                                                                                                                                                                                                                        gCTGGATACGTTACCCAATCAAACCAGCGCCCACCTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCGAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCAAACGGCTTCTATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGt                                                                                                                   >  2:202444/1‑247 (MQ=255)                                                                                                                                                                                                                                        gCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCt                                                              >  2:472825/1‑300 (MQ=255)                                                                                                                                                                                                                                           ggATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGAc                                                           >  1:269249/1‑300 (MQ=255)                                                                                                                                                                                                                                             aTACATTACAAAATCAAACCAGCGCCAAACTGACCCGCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATGTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGTGATTGGTGTCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGaac                                                                                                    <  2:64851/257‑2 (MQ=255)                                                                                                                                                                                                                                                                         aCTGACCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGGCACGtt                             <  2:75839/300‑1 (MQ=255)                                                                                                                                                                                                                                                                             aCCCTCGACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGGCACGTTAGTg                         <  1:717426/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   gACT‑GCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGGCACGTTAGTGAGTAt                    <  2:410240/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                        gccgccTGCCAACCCTGNTACTGGATGCGCAAATGCNGGTGCATGTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGGCACNTTAGTGAGTATTAGCt               <  1:599337/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        gCCGTCTGCCAACCCTGGTACTTGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGGCACGTTAGTGAGTATTAGCt               <  2:686155/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              tGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGGCACGTTAGTGAGTATTAGCTTTCGct         >  1:24862/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                   aCCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGGCACGTTAGTGAGTATTAGCTTTCGCTCTGc     >  1:683664/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                    cccTGGTACTGGATGCGCAAATGTAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCAAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGGCACGTTAGTGAGTATTAGCTTTCGCTCTGc     >  2:308266/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                                      cTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAATAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGGCACGTTAGTGAGTATTAGCTTTCGCTCTGCGGa  >  1:354519/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                       GCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCAT‑TGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACT‑GCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGGGACGCTGACTTTTTCACAACCTTCCGGCGGCGGCACGTTAGTGAGTATTAGCTTTCGCTCTGCGGA  >  NC_000913/2586817‑2587406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑