Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,348,366	C→A	T328K (ACG→AAG)	nrdB →	ribonucleoside‑diphosphate reductase 1 subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,348,366	0	C	A	100.0%	192.9 / NA	56	T328K (ACG→AAG)	nrdB	ribonucleoside‑diphosphate reductase 1 subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/34);  total (22/34)

GAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGACGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAATGTGGCGGTTGAGTACCAGTGTCGCGAAGGTTACTGCGGCTCCTGTCGCACA  >  NC_000913/2348098‑2348654                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                 gaAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAacac                                                                                                                                                                                                                                                                    <  1:18681/299‑1 (MQ=255)    gCCCTGCACCTGACCGGCACCTAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACtt                                                                                                                                                                                                                                                                  >  2:352144/1‑298 (MQ=255)    gCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACtt                                                                                                                                                                                                                                                                  >  1:240447/1‑298 (MQ=255)    gCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCAGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACtt                                                                                                                                                                                                                                                                  >  2:426806/1‑298 (MQ=255)    gCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCAACAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACtt                                                                                                                                                                                                                                                                  >  1:367240/1‑298 (MQ=255)        tGCACCTGACCTGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCTAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGATAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGc                                                                                                                                                                                                                                                               <  2:26211/297‑1 (MQ=255)        tGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTTAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGGCCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACtttgc                                                                                                                                                                                                                                                               <  1:30572/297‑4 (MQ=255)        tGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGc                                                                                                                                                                                                                                                               <  1:31064/297‑1 (MQ=255)          cACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACNTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACNGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGgt                                                                                                                                                                                                                                                           >  2:543303/1‑299 (MQ=255)            ccTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGCAGCGCTCCAACCCGATCCCGTGGCTCAACACTTGGCTGGTGTc                                                                                                                                                                                                                                                        >  2:658311/1‑300 (MQ=255)            ccTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGACGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCAACAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCAAAAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTc                                                                                                                                                                                                                                                        >  2:339416/1‑300 (MQ=255)                aCCGGCACCCAGCATATNCTGAATCTGCTGCGCAGCNGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACANTTGGCTGGTGTCTGAt                                                                                                                                                                                                                                                    <  1:605593/300‑1 (MQ=255)                   ggCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATaa                                                                                                                                                                                                                                                  <  2:577922/299‑1 (MQ=255)                   ggCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATaa                                                                                                                                                                                                                                                  <  1:235458/299‑1 (MQ=255)                    gCACCCAGCATATGCTGAATCGGCGGCGCAGCGGCGCGGACAATCCGGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGTTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCATGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGagaa                                                                                                                                                                                                                                                  <  2:388440/298‑4 (MQ=255)                          aGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGAACTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCa                                                                                                                                                                                                                                            >  2:90890/1‑298 (MQ=255)                          aGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGAGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCAATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGCATACATCAACAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGTGCTCCAACCCGATCCCGTGGATCa                                                                                                                                                                                                                                                                        >  2:210606/1‑270 (MQ=255)                             atatGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGt                                                                                                                                                                                                                                         <  2:400663/298‑1 (MQ=255)                                tGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGCCGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCAAGAAGCGCTCCAAACCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTc                                                                                                                                                                                                                                    >  2:493319/1‑300 (MQ=255)                                tGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCAACAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCCACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTc                                                                                                                                                                                                                                    >  2:157260/1‑300 (MQ=255)                                tGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTCCAGGCAGCTCAACAGGAGAAAGACTGGGCGGGTTATCTGTTCAGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCAACAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCACAACTTGGCTGGTGTCTGATAACGTGCAGGCTGCTc                                                                                                                                                                                                                                    >  2:157128/1‑300 (MQ=255)                                 gCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTcc                                                                                                                                                                                                                                   <  1:214513/300‑1 (MQ=255)                                                           aCGATCCTGAGATGGCGGAAATTGCCTAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGTAGAAAGACTGTGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGTTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATc                                                                                                                                                                                                         <  2:262713/300‑1 (MQ=255)                                                             gATCCTGAGATTGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACATGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCATGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCTTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATc                                                                                                                                                                                                         <  2:263610/298‑1 (MQ=255)                                                                                        gagTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCACCCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGac                                                                                                                                                                            <  2:393010/300‑1 (MQ=255)                                                                                          gtttAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCAACAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGacac                                                                                                                                                                          >  1:639164/4‑300 (MQ=255)                                                                                          gtgtAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATTTGGTCGGGCAGATTGACTCGGCAGTGGaca                                                                                                                                                                           >  2:192706/1‑299 (MQ=255)                                                                                              aaGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACcgac                                                                                                                                                                      >  1:425048/1‑300 (MQ=255)                                                                                                                 tgtttgttCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGc                                                                                                                                                   <  1:634723/300‑1 (MQ=255)                                                                                                                  gtttgttCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGTGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGc                                                                                                                                                   <  1:634733/299‑1 (MQ=255)                                                                                                                   tttgttCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGctc                                                                                                                                                 <  1:615073/300‑1 (MQ=255)                                                                                                                        tCAGGCAGCTCAACAGTAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATg                                                                                                                                            <  1:473868/300‑1 (MQ=255)                                                                                                                               gCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCAACAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGANCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTAAGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACCCCGACGATTTGAGTAACTTCCAGCTCTGATGGc                                                                                                                                          >  2:223102/1‑295 (MQ=255)                                                                                                                                      atgagaAAGACTGGGCGGATTATCTGTTCCGCGACGGGTCGATGATGGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCATAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTAccc                                                                                                                               <  2:374180/297‑1 (MQ=255)                                                                                                                                      agtagaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTAccc                                                                                                                               <  1:209307/296‑1 (MQ=255)                                                                                                                                      aGGAGAGAGACTGGGCGGATTATCTGTTCCGTGACGGTGTGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTAccc                                                                                                                               <  2:391832/299‑1 (MQ=255)                                                                                                                                      aGGAGAAAGACTGTGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTAccc                                                                                                                               <  2:379695/299‑1 (MQ=255)                                                                                                                                      aGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGTGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTAccc                                                                                                                               <  2:409450/299‑1 (MQ=255)                                                                                                                                      aGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTAccc                                                                                                                               <  1:209321/299‑1 (MQ=255)                                                                                                                                       ggAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACCCTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGAATCGGAAGTGGACAACGACGATTTGAGTAACTTc                                                                                                                                                       >  2:60045/1‑274 (MQ=255)                                                                                                                                        gagaAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTAcccct                                                                                                                             >  1:379695/1‑297 (MQ=255)                                                                                                                                                       gATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGcacac                                                                                                             >  1:10563/1‑300 (MQ=255)                                                                                                                                                              tGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAActgc                                                                                                       <  1:92391/299‑1 (MQ=255)                                                                                                                                                                                      gtctggagaaaGACATTCTCTGCCAGTACGTTGAATACATCACCGATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAATTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACAcccttccct                                                                              <  2:477487/292‑1 (MQ=255)                                                                                                                                                                                          gAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCt                                                                           >  1:75415/1‑299 (MQ=255)                                                                                                                                                                                          gAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTNCTTATCTGGTCGGGCAGCTTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACAcccttcccttat                                                                           >  2:224312/1‑297 (MQ=255)                                                                                                                                                                                                              aGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGNTTGACTCGGAAGTGGACACCGACGATTTGAGTNACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACaa                                                      <  2:539209/300‑1 (MQ=255)                                                                                                                                                                                                               gTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAAt                                                     <  1:383006/300‑1 (MQ=255)                                                                                                                                                                                                                 aCGTTGAATACATCAACAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACaa                                                      >  2:51859/1‑297 (MQ=255)                                                                                                                                                                                                                   gTTGAATACATCACCGGTATCTGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGTTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAAtgt                                                   <  2:112785/298‑1 (MQ=255)                                                                                                                                                                                                                                   atatCCGTATGCATGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAATGTGGCGGTTGAGTACCAgtg                                 <  1:658311/300‑1 (MQ=255)                                                                                                                                                                                                                                   atatCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAATGTGGCGGTTGAGTACCAgtg                                 <  1:339416/300‑1 (MQ=255)                                                                                                                                                                                                                                                   agTCGGTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAATGTGGCGGTTGAGTACCAGTGTCGCGAAGGTTACTGc                 <  1:493319/300‑1 (MQ=255)                                                                                                                                                                                                                                                   agTCGGTTTGGATCTGCCGTGCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAATGTGGCGGTTGAGTACCAGTGTCGCGAAGGTTACTGc                 <  2:656677/300‑1 (MQ=255)                                                                                                                                                                                                                                                        gTTTGGATCTGCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAATGTGGCGGTTGAGTACCAGTGTCGCGAAGGTTACTGCGGCTc            <  1:426806/300‑1 (MQ=255)                                                                                                                                                                                                                                                                  gCCGTTCCAGAAGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAATGTGGCGGTTGAGTACCAGTGTCGCGAAGGTTACTGCGGCTCCTGTCGcaca  <  1:543303/300‑1 (MQ=255)                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                 GAAGCCCTGCACCTGACCGGCACCCAGCATATGCTGAATCTGCTGCGCAGCGGCGCGGACGATCCTGAGATGGCGGAAATTGCCGAAGAGTGTAAGCAGGAGTGCTATGACCTGTTTGTTCAGGCAGCTCAACAGGAGAAAGACTGGGCGGATTATCTGTTCCGCGACGGTTCGATGATTGGTCTGAATAAAGACATTCTCTGCCAGTACGTTGAATACATCACCAATATCCGTATGCAGGCAGTCGGTTTGGATCTGCCGTTCCAGACGCGCTCCAACCCGATCCCGTGGATCAACACTTGGCTGGTGTCTGATAACGTGCAGGTTGCTCCGCAGGAAGTGGAAGTCAGTTCTTATCTGGTCGGGCAGATTGACTCGGAAGTGGACACCGACGATTTGAGTAACTTCCAGCTCTGATGGCCCGCGTTACCCTGCGCATCACTGGCACACAACTGCTGTGCCAGGATGAACACCCTTCCCTTCTGGCGGCGCTGGAATCCCACAATGTGGCGGTTGAGTACCAGTGTCGCGAAGGTTACTGCGGCTCCTGTCGCACA  >  NC_000913/2348098‑2348654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑