| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 59,968 | C→T | V127I (GTC→ATC) | rluA ← | 23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 59,968 | 0 | C | T | 98.2% | 174.3 / NA | 55 | V127I (GTC→ATC) | rluA | 23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (23/31); total (24/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCG > NC_000913/59712‑60257 | gTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGa > 1:434061/1‑300 (MQ=255) cTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACANGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCAACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATccacc > 2:529589/1‑300 (MQ=255) cTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATccacc > 1:386713/1‑300 (MQ=255) ccATACGCCGGATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCt > 1:436585/1‑300 (MQ=255) ggATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGc > 1:717117/1‑296 (MQ=255) ggATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCgggg < 2:408903/300‑1 (MQ=255) ggTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGggat > 1:661137/1‑298 (MQ=255) aaTCTTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCGTCGCTCTCGCTTCTGTTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCTGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGgatggatg < 2:194108/300‑1 (MQ=255) aaTCGTCAACATCTCTGCATTTAGCAACAAACTTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGgatggatg < 2:108284/300‑1 (MQ=255) aaTCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGgatggatg < 1:364627/300‑1 (MQ=255) aTCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGAcg < 1:31410/300‑1 (MQ=255) aTCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGAcg < 2:310534/300‑1 (MQ=255) gCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGcc > 1:426623/1‑300 (MQ=255) aGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGTTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATAc < 2:94690/300‑1 (MQ=255) aGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGTTGATGCATAAAAACGATCGACGAGAAGCGGACGACCCAGCGCCATCATATGCACACGCGATTGGTGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCTCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATAc < 2:93073/300‑1 (MQ=255) aGCAACAAACGTGGTGCCATAGCTCTCGCTTCTGGTGATGCATAAAAACGACCGCCGAGAATCGGATGACCCAGCGCCAGCATAGGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGTGGATGGATGCCCCCAGACGCGGGCCACATAc < 2:297644/300‑1 (MQ=255) gCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGgctcgcgctc > 1:130870/1‑299 (MQ=255) gCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCAATTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGgctcgcgctc > 1:131756/1‑299 (MQ=255) gCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCAATTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCACAGCCGCGGGCCACATACTGCTTTTTCGgctcgcgctc > 1:130751/1‑299 (MQ=255) tCTGGTGATGCATAAAAACGATCGCCGAGAATCGTATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACt > 1:412988/1‑298 (MQ=255) aTGCATAAAAACGATCGCAGAGAATCGGATGACCCCGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTCCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGTGGGGGATGGATGCCCCCAGACGCGGGCCACATAc < 2:93719/263‑1 (MQ=255) aCGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTc < 1:636238/300‑1 (MQ=255) aGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCAGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTTCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTa < 2:159446/298‑1 (MQ=255) gcgcCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGTTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACaa < 1:91156/300‑1 (MQ=255) gcgcCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACaa > 1:445610/1‑300 (MQ=255) aGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCTGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCAc > 1:432699/1‑299 (MQ=255) acacGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCTGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTgg < 2:309804/298‑1 (MQ=255) acGCAATTGATGCGAACGCCCTGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGc < 2:188549/300‑1 (MQ=255) gAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACg < 1:547794/299‑1 (MQ=255) aacacccGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACg < 2:276621/294‑1 (MQ=255) ggTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGa < 1:163116/300‑1 (MQ=255) tAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGAtt < 1:688848/300‑1 (MQ=255) tAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACCCCACCACTTCATATTCCTTCTGCGCAGGTTTACCCATTTCGTAACAGATTTCC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGTGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGTTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGAtt < 2:314462/300‑1 (MQ=255) aaTCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGAtt < 2:314427/299‑1 (MQ=255) ggTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTctgcc < 2:445610/300‑1 (MQ=255) gTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCAATTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCAGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCCGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAATTGGCGTTTTAAATCACGATCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTatgcct > 2:501634/1‑300 (MQ=255) caccacTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTCCCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCCGTCGCAAATCAGCGGCAGATCCCCCAGACCTTCTGCGGGGGATGGATGCACCCAGACGCGGGCCACGTACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGACTCTGCATGCGGAt > 2:81276/1‑299 (MQ=255) gtgtTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAAt > 1:488504/1‑299 (MQ=255) gtgtTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCAAGCTGAAt > 1:442567/1‑299 (MQ=255) gccgcATACTCCACCAGTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGtcatc > 2:664037/1‑300 (MQ=255) gccgcATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGtcatc > 2:682995/1‑300 (MQ=255) aTACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCNGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGc < 1:147869/299‑1 (MQ=255) cTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCAAGCTGAATGCGCGtaatcaag > 2:335145/1‑293 (MQ=255) cTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCACAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACg > 2:312083/1‑295 (MQ=255) acTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGTGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGc < 2:717117/300‑1 (MQ=255) acTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGANCTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTtgtg < 2:521377/299‑1 (MQ=255) ttCATATTCCGTCTGCGGAGGTTTACCCGTTTCGTAAAAG‑CTTTCCTGTCTCGGGCGGTTTGGCCAGTCGCAAATCAGCGACAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCACAGACGCGGGCCACATCTTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCAAATACAATCACGCCGCTGGTATCCATATCCAGACGATGCACCGATTCTGCCTGTGGATAATCACGCTGACTGCGCGTAATCACGCCGTCTTTGTGcta > 2:98834/1‑299 (MQ=255) ttCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGTTGTCTTTGTGctc > 2:99160/1‑300 (MQ=255) ttCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCAGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACCGATTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCCGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCAAGCTGTCTTTGTGctc > 2:99779/1‑300 (MQ=255) atgTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGTGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCAc < 2:332371/298‑1 (MQ=255) aGGTTTACCCGTTTCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCAc < 1:335145/300‑1 (MQ=255) ttCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCTGCAGATCCACCAGACCTTCTGCGGTGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAAcc < 1:98834/300‑1 (MQ=255) ttCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAAcc < 1:99160/300‑1 (MQ=255) ttCGTAACAGATTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGGTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGACACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTTATTACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAAcc < 1:99779/300‑1 (MQ=255) tCGTAGCAGATTTTC‑TGTTTCGGTTGGTTTGGCCAGTCGCAAATCAGCTGCAGATCCCCCAGACTTTCTGCTTGGGATGGATTCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCTCGGCTTTGTTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCAGATAATCACGCTGAATGCACGTCATCACACTTTATTGGGGAGCTGCCAGACGACCCGGCACTGACAACAAACCg < 2:436585/300‑1 (MQ=255) | GTCATACTATTGCCATACGCCGGATGGGTAATCGTCAACATCTCTGCATGTAGCAACAAACGTGGTGCCATCGCTCTCGCTTCTGGTGATGCATAAAAACGATCGCCGAGAATCGGATGACCCAGCGCCAGCATATGCACACGCAATTGATGCGAACGCCCGGTAATCGGTTTTAACACCACTCTTGCCGTGTTATCCGCCGCATACTCCACCACTTCATATTCCGTCTGCGCAGGTTTACCCGTTTCGTAACAGACTTTC‑TGTTTCGGGCGGTTTGGCCAGTCGCAAATCAGCGGCAGATCCACCAGACCTTCTGCGGGGGATGGATGCCCCCAGACGCGGGCCACATACTGCTTTTTCGGCTCGCGCTCGCGGAACTGGCGTTTTAACTCCCGCTCCGCGGCTTTGGTCAGCGCCACTACAATCACGCCGCTGGTAGCCATATCCAGACGATGCACCGATTCTGCCTGCGGATAATCACGCTGAATGCGCGTCATCACGCTGTCTTTGTGCTCTTCCAGACGACCCGGCACTGACAACAAACCG > NC_000913/59712‑60257 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |