Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,100	G→A	E172K (GAA→AAA)	ydiF →	putative acetate‑CoA transferase YdiF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,100	0	G	A	100.0%	268.9 / NA	77	E172K (GAA→AAA)	ydiF	putative acetate‑CoA transferase YdiF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/39);  total (38/39)

GTATTAGTCCTCTGGCGCAAGAAGGTCTGGTGAAATGGGCATTATGTGGTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAGAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCACCGG  >  NC_000913/1775819‑1776379                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                         gTATTAGTCCTCTGGCNCAAGAAGGTCTGGTGAAATGGGCATTATGTGGTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTGNGTCGAGTTTGATAACAAANAAATATCTCTAtgtaca                                                                                                                                                                                                                                                                         >  1:597635/1‑295 (MQ=255) gTATTAGTCCTCTGGCGCAAGAAGGTCTGGTGAAATGGGCATTATGTGGTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAACTTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGGTAACAAAAAC‑TATCTCTATTACaaa                                                                                                                                                                                                                                                                        >  2:156888/1‑299 (MQ=255) gTATTAGTCCTCTGGCGCAAGAAGGTCTGGTGAAATGGGCATTATGTGGTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTCAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTAaaaa                                                                                                                                                                                                                                                                        >  2:157652/1‑299 (MQ=255) gTATTAGTCCTCTGGCGCAAGAAGGTCTGGTGAAATGGGCATTATGTGGTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACaaa                                                                                                                                                                                                                                                                        >  1:286451/1‑299 (MQ=255) gTATTAGTCCTCTGGCGCAAGAAGGTCTGGTGAAATGGGCATTATGTGGTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGAACTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTATATTACaaa                                                                                                                                                                                                                                                                        >  1:539427/1‑299 (MQ=255)       gTCCTCTGGCGCAAGAAGGTCTGGTGAAATGGGCATTATGTGGTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAANTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGAtt                                                                                                                                                                                                                                                                  >  1:526488/1‑299 (MQ=255)              ggcacAAGAAGGTCTGGTGAAATGGGCATTATGTGGTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCa                                                                                                                                                                                                                                                            >  1:287543/5‑298 (MQ=255)              ggCGCAAGAAGGTCTGGTGAAATGGGCATTATGTGGTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTATATTACAAAGCGATTGCGCCAGa                                                                                                                                                                                                                                                          >  1:285951/1‑300 (MQ=255)               gcgcAAGAAGGTCTGTTGAAATGGGCATTATGTGGTCACTGTGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAATGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGat                                                                                                                                                                                                                                                         <  2:452900/300‑1 (MQ=255)                    agaagGTCTGGTGAAATGGGCATTATGTGGTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGc                                                                                                                                                                                                                                                    <  1:384513/300‑1 (MQ=255)                    agaagGTCTGGTGAAATGGGCATTATGGGGTCACTGTGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCTGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGc                                                                                                                                                                                                                                                    <  2:285951/300‑1 (MQ=255)                    agaagGGCTGGTGAAATGGGCATTAGGTGGTCACTGTGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACGGAATGAAGGCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGc                                                                                                                                                                                                                                                    <  2:287543/300‑1 (MQ=255)                      aagGTCTGGTGAAAGGGGCATTATGTGGTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCt                                                                                                                                                                                                                                                  <  2:384924/300‑1 (MQ=255)                         gTCTGGTGGAATGGGCATTATGTGGTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAACGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGAttacggcaggcctttccttca                                                                                                                                                                                                                                               >  2:165462/1‑281 (MQ=255)                                 aaaTGGGCATTATGTGGTCACTGTGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑TTCGAGTTTGATAACAAAAAA‑TACCTCTATTACAAAACGATTACGccctgtctcttatacacatctcgc                                                                                                                                                                                                                                       <  2:450295/300‑23 (MQ=255)                                  aaTGGGCATTATGTGGTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCAATAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTATATTACAAAGCGATTGCGCAAGATATTGCCTTCATTCGCGCt                                                                                                                                                                                                                                       >  2:280620/1‑299 (MQ=255)                                     gggCATTATGTGGTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATACAATTATTGCTTCTAACTACCCCCAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCATGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTacca                                                                                                                                                                                                                                   >  1:363049/1‑300 (MQ=255)                                     gggCATTATGTGGTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTacca                                                                                                                                                                                                                                   >  1:362764/1‑300 (MQ=255)                                          ttATGTGGTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGc                                                                                                                                                                                                                              >  2:358755/1‑300 (MQ=255)                                                    aCTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGaa                                                                                                                                                                                                                     >  2:504477/1‑299 (MQ=255)                                                    aCTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCACTACCACCCGCGACAGTGa                                                                                                                                                                                                                      >  2:354383/1‑298 (MQ=255)                                                    aCTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTATATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGa                                                                                                                                                                                                                      >  1:41770/1‑298 (MQ=255)                                                      tgtggACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCTGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGc                                                                                                                                                                                                                  <  2:297673/297‑1 (MQ=255)                                                       ggggACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGAAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAAAAAAAAA‑TATTTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCt                                                                                                                                                                                                                 >  2:649934/1‑300 (MQ=255)                                                                         tttCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTTATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATNACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGAt                                                                                                                                                                                                >  1:152302/1‑299 (MQ=255)                                                                         tttCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGACGAt                                                                                                                                                                                                >  1:630687/1‑299 (MQ=255)                                                                                      aGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATTAGGTGATGTAtct                                                                                                                                                                                  <  2:624230/300‑1 (MQ=255)                                                                                      aGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTAtct                                                                                                                                                                                  <  2:450469/300‑1 (MQ=255)                                                                                                     tattGCTTATAACTACCCATAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCTGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GNCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTAt                                                                                                                                                                   <  2:511462/300‑1 (MQ=255)                                                                                                         gCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGcc                                                                                                                                                               >  1:470776/1‑300 (MQ=255)                                                                                                              taACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGc                                                                                                                                                          >  1:664044/1‑300 (MQ=255)                                                                                                                aCTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCgg                                                                                                                                                        <  1:51691/300‑1 (MQ=255)                                                                                                                   aCCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGc                                                                                                                                                     >  1:77169/1‑300 (MQ=255)                                                                                                                    cccACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGANCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCACAGGCGGTGca                                                                                                                                                    >  2:525376/1‑300 (MQ=255)                                                                                                                         aaGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCTGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGTTGATATATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAAt                                                                                                                                                <  1:291463/299‑1 (MQ=255)                                                                                                                         aaGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATATATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAAt                                                                                                                                                <  1:696894/299‑1 (MQ=255)                                                                                                                          aGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑TTCGAGGTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATaa                                                                                                                                              <  2:260638/300‑1 (MQ=255)                                                                                                                           ggTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTATATTACAAAGCGATTGCGCCAGATATTGCATTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAAc                                                                                                                                             >  2:37754/1‑300 (MQ=255)                                                                                                                               tACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCAACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTATATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTAGACGCATTGGTTATTGCCCAGGCGGTGCACAATAAcggc                                                                                                                                          >  2:20760/1‑299 (MQ=255)                                                                                                                                aCTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGGTGAGGTGATGTATCTAGAAGCATTGGTTATTGCCCAGGCGGTGCCCAATAAc                                                                                                                                             >  2:187727/1‑295 (MQ=255)                                                                                                                                aCTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGACACTTTTGAAGATGAGGTGATGTATCTCGAAGCATCGGTTATTGACCAGGCGGTGCACAATCAcggc                                                                                                                                          >  2:187800/1‑298 (MQ=255)                                                                                                                                     cacaAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTAtt                                                                                                                                    >  1:457394/1‑299 (MQ=255)                                                                                                                                      acgaaCCTTACGCGCCGCCGCAGCCCACTAGCCTGGTAGTATTAGTGATATTGGTATTGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCAAGAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAGGGCTACGCCACTTTTGAAGATTGGTTGATGTATCTCGACGTATTGGTTATTGCCCAGGCGGTGCATAATAACGGCGGTATtg                                                                                                                                   <  2:397795/296‑1 (MQ=255)                                                                                                                                        aaaCCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAANCGATTGCGCCAGATATTGCCTTCATTCGCGCTNCCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGTTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGtga                                                                                                                                <  2:537471/300‑1 (MQ=255)                                                                                                                                          aCCTTACGCGCCGCCACAGCCCACCAGCCTGGTATTATTATTGATATGGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGTGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTACCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGtgatg                                                                                                                              <  2:286451/300‑1 (MQ=255)                                                                                                                                                 gctccgccgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACGGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGNCAGATATTGCCTTCATTCGCGCTACCACCTGCNACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAgg                                                                                                                       <  2:546276/297‑1 (MQ=255)                                                                                                                                                       ccgcAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGa                                                                                                                 >  1:348733/1‑300 (MQ=255)                                                                                                                                                               caccaGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTAAGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGtt                                                                                                         >  2:142764/1‑300 (MQ=255)                                                                                                                                                                         gTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCa                                                                                               >  2:412164/1‑300 (MQ=255)                                                                                                                                                                                  gTGATATTGGCATCTGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGTTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATc                                                                                      <  1:591200/300‑1 (MQ=255)                                                                                                                                                                                    gATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTATATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGANGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCt                                                                                    >  2:564017/1‑300 (MQ=255)                                                                                                                                                                                         tgtcATCTGGACATTTGTTGAGCCACGCCAGCGAGGCGGCAAACGGAATGAAATGACTAAAGAAGACCTAATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TACCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAAATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATTCTAAATc                                                                               <  2:460932/297‑1 (MQ=255)                                                                                                                                                                                         tGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTN‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGTTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATc                                                                               <  1:157652/300‑1 (MQ=255)                                                                                                                                                                                         tGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTN‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATc                                                                               <  1:156888/300‑1 (MQ=255)                                                                                                                                                                                         tGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACATTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTTTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATc                                                                               <  2:139339/300‑1 (MQ=255)                                                                                                                                                                                         tGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATc                                                                               <  1:412164/300‑1 (MQ=255)                                                                                                                                                                                                  gACATTTGTCGATCCACGCCAGCAAGTCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACGG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTTCGACATTGAAGGCTACGCCACTTTTGAAGATTAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAATCCACGCTGCATCCTAAATCTGTCCGTAt                                                                      <  2:359035/300‑1 (MQ=255)                                                                                                                                                                                                              tCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑TTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCt                                                          <  1:504477/300‑1 (MQ=255)                                                                                                                                                                                                              tCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCt                                                          <  1:464601/300‑1 (MQ=255)                                                                                                                                                                                                                  cGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCtggt                                                       >  1:414047/1‑299 (MQ=255)                                                                                                                                                                                                                          agtcggcAAACTGAATGAAGTCACTAAAGGAGACCGGATTAAGCTT‑GTCGAGGTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTACGCCAGATATTGCCTTCATTCGCACTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGTCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATtgt                                              <  2:363049/297‑1 (MQ=255)                                                                                                                                                                                                                          aGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGAGAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATTAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATtgt                                              <  2:362764/300‑1 (MQ=255)                                                                                                                                                                                                                               gcAAACTGAATGAAGTCACTAAAGAAGACCCGATTAAACTG‑TTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAATCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTgtgg                                            <  2:77169/297‑1 (MQ=255)                                                                                                                                                                                                                                      gaatgaaGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCATTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTCTTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAACTCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTTGATc                                   >  2:201522/1‑299 (MQ=255)                                                                                                                                                                                                                                                             tGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGc            >  1:450884/1‑299 (MQ=255)                                                                                                                                                                                                                                                             tGATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGc            <  1:20760/299‑1 (MQ=255)                                                                                                                                                                                                                                                              gagtAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCgg          <  2:457394/297‑1 (MQ=255)                                                                                                                                                                                                                                                              gATTAAACTG‑TTCGAGTTTGAGAACAAAAAA‑TATCTCTATTACAAAGCAATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTACGACAGTGAAGGCTACGCCACTTTTGAAAATGAGGTAATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCgg          <  2:43653/300‑1 (MQ=255)                                                                                                                                                                                                                                                              gATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGANGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGc            >  2:512123/1‑298 (MQ=255)                                                                                                                                                                                                                                                              gATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACACTCACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCAGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACGGTATGGc            >  2:576730/1‑298 (MQ=255)                                                                                                                                                                                                                                                              gATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCgg          <  1:191476/300‑1 (MQ=255)                                                                                                                                                                                                                                                              gATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATATATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCgg          <  1:358755/300‑1 (MQ=255)                                                                                                                                                                                                                                                              gATTAAACTG‑GTCGAGTTTGATAACAAAAAA‑TATCTATATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCTAGGCGGTGCACAATAACGGTGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTATGGTGGTCGATCCGGATCAAACCCCACTGTAt               >  2:37039/1‑295 (MQ=255)                                                                                                                                                                                                                                                              gATTAAACTG‑GTCGAGTTGGATAACAAAAAC‑TATCTCTATTACAGAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATTAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCgg          <  2:597635/300‑1 (MQ=255)                                                                                                                                                                                                                                                              gATTAAACTG‑GTCGAGNTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGNGGTGGTCGATCCGGATCANACCCAACTGTATGGc            >  1:605621/1‑298 (MQ=255)                                                                                                                                                                                                                                                              gATTAAACTA‑TTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCACGCTACCACCTGCGACAGTGAAGGCTACTCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCgg          <  2:123645/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   aaCTG‑GTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATATCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCAc     <  1:482404/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      gg‑gTCGAGTTTGATAACAAAAAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCNGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCACCgg  <  2:526488/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                         GTATTAGTCCTCTGGCGCAAGAAGGTCTGGTGAAATGGGCATTATGTGGTCACTGGGGACAATCGCCGCGTATTTCTGAACTCGCAGAACAAAATAAAATTATTGCTTATAACTACCCACAAGGTGTACTTACACAAACCTTACGCGCCGCCGCAGCCCACCAGCCTGGTATTATTAGTGATATTGGCATCGGGACATTTGTCGATCCACGCCAGCAAGGCGGCAAACTGAATGAAGTCACTAAAGAAGACCTGATTAAACTG‑GTCGAGTTTGATAACAAAGAA‑TATCTCTATTACAAAGCGATTGCGCCAGATATTGCCTTCATTCGCGCTACCACCTGCGACAGTGAAGGCTACGCCACTTTTGAAGATGAGGTGATGTATCTCGACGCATTGGTTATTGCCCAGGCGGTGCACAATAACGGCGGTATTGTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCACCGG  >  NC_000913/1775819‑1776379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑