Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,322,605	C→T	intergenic (‑183/+731)	phnF ← / ← phnD	putative transcriptional regulator PhnF/phosphonate/phosphate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,322,605	0	C	T	100.0%	182.8 / NA	54	intergenic (‑183/+731)	phnF/phnD	putative transcriptional regulator PhnF/phosphonate/phosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/37);  total (17/37)

GTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTCGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCA  >  NC_000913/4322336‑4322851                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                       gTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATag                                                                                                                                                                                                                          <  1:51402/300‑1 (MQ=255) gTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATa                                                                                                                                                                                                                           >  2:613543/1‑299 (MQ=255)  tAGTGTTGACGAAACTCCTGCTCAATTTTTTCTGCTATCTCTTGATAGCGTTTTTGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCTTTGAGAGAGGAAATCCAGCAGGCTGACNGTGACGATGATAAGCACCATCAGGGCGCAGGTNTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGTTGACGCCGATCCCGCCTGCGCTGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAg                                                                                                                                                                                                                                <  2:538728/293‑1 (MQ=255)    gtgtTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATaga                                                                                                                                                                                                                         >  1:679661/1‑298 (MQ=255)      gtTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGa                                                                                                                                                                                                                     >  2:171403/1‑300 (MQ=255)        tGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGt                                                                                                                                                                                                                   <  1:495200/300‑1 (MQ=255)          acgtaGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGATAGGAAATCCAGCAGGCTGACCTTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATagag                                                                                                                                                                                                                        <  2:106373/289‑1 (MQ=255)             aaGCTCCTGCTCAATTTTTGCGGCTATCTCTTGATAGCGTGTTGGTTAGCTGGTCGGATGTGTAGACAAGTGCATANATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAgg                                                                                                                                                                                                                <  1:525960/298‑1 (MQ=255)             aaGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGgag                                                                                                                                                                                                              <  1:6352/300‑1 (MQ=255)                         aaGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCTAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTgg                                                                                                                                                                                                   <  2:122679/299‑1 (MQ=255)                               tGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCAcc                                                                                                                                                                                            <  2:467607/300‑1 (MQ=255)                               tGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGNTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCAcc                                                                                                                                                                                            <  2:516836/300‑1 (MQ=255)                                     tATCTCTTGATAGCGTGTTGGTTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGgc                                                                                                                                                                                      <  2:150685/300‑1 (MQ=255)                                     tATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGgc                                                                                                                                                                                      <  1:276252/300‑1 (MQ=255)                                          ctTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCAc                                                                                                                                                                                 >  1:564138/1‑300 (MQ=255)                                               tAGCGTGTTGTGTAGCTTGTCTGATGTGTAGACAAGTGCAGAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGCCGCAGGTTGTTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGTTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGATCGGACGTTGTATTCGAAGCGATAGAGGGAGTAGGAGATCAGCATTGGCATCACCTGTGGCAGCACGCCGt                                                                                                                                                                            <  2:193757/300‑1 (MQ=255)                                               tAGCGTGTTGGGTAGCTGTTCGGATGTGTAGAAAAGTGTAGACATATCAATGTCCCTCTTAGCAAATAAAATACTTGCGCAACCGTTGAGAGGGGGAATCCCACAGGCTGACCTTTACGACGATAAACACCCCCAGTGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCAGGCCGACGACGGTTGCCGAGCGGACCTTGTATTCGAAGCGATAGAGGGAGTAGTAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGt                                                                                                                                                                            <  2:194813/300‑1 (MQ=255)                                                    tgtTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAgg                                                                                                                                                                       <  1:422717/300‑1 (MQ=255)                                                     gtTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATGTCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGTAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACTGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCGGCAGTGGCATCACATGTGGCAGAACGCAGTAGAGGa                                                                                                                                                                      >  2:415470/1‑300 (MQ=255)                                                      ttggttaacTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGTGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGAt                                                                                                                                                                     <  2:259764/292‑1 (MQ=255)                                                       tGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGAtc                                                                                                                                                                    <  1:233620/300‑1 (MQ=255)                                                        gggTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGAtct                                                                                                                                                                   >  1:56244/1‑300 (MQ=255)                                                                ggTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGc                                                                                                                                                           <  2:604149/300‑1 (MQ=255)                                                                ggTCGGATGGGTAGACAAGTGCATAGATATCAATGCCTCGCTGATCAGATAAAGTGCTTACGCAACCGTTGAGATAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGTAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGc                                                                                                                                                           <  2:250422/300‑1 (MQ=255)                                                                        gtgtAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAANCCGCGAATCGCTTCCCACAGGGTGACGCCGCTCCCGCCTGCGCCGCCCATGCGAACGACGGTTGCCGAGCGGGGGTTGGATTCGAAGCGATAGCGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCGTCGAgt                                                                                                                                                           >  2:531695/1‑291 (MQ=255)                                                                        ggctAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTCACCCAACCGTTAAGAGAGANAATCCAGCAGGCTGACCGCAACGATGACAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGc                                                                                                                                                           <  1:531695/289‑1 (MQ=255)                                                                          gtAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCANGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCAc                                                                                                                                                 <  1:137021/300‑1 (MQ=255)                                                                          gtAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCAc                                                                                                                                                 <  1:695292/300‑1 (MQ=255)                                                                                         gATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGc                                                                                                                                  >  1:127543/1‑300 (MQ=255)                                                                                                aTGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCAc                                                                                                                           <  1:44791/300‑1 (MQ=255)                                                                                                     tCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCAccgggc                                                                                                                      <  1:344633/300‑1 (MQ=255)                                                                                                        cTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGCAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTACAccgggcc                                                                                                                     >  2:359644/1‑298 (MQ=255)                                                                                                                          tACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGANCTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCg                                                                                                 <  1:524494/300‑1 (MQ=255)                                                                                                                                aCCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCTAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCAccgg                                                                                                                                                                                          <  2:283064/205‑3 (MQ=255)                                                                                                                                aCCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCtg                                                                                                                                                                                          >  1:283064/1‑205 (MQ=255)                                                                                                                                aCCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCACTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGCTGCCGAGCGGACGTTGGATTCGAAGCAATAGAGGGAGTAGGCGATCAGCAGTGGCATCCCCTGTGGCAGCACGCCGTAGGGGATCTACTCGAGCTTGTTGGCCCCGGTGGAGAGAATGCCTTCAACCGGGACGGGCTCCATCTCtt                                                                                                       >  2:181641/1‑288 (MQ=255)                                                                                                                                 ccGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGa                                                                                          >  1:655918/1‑300 (MQ=255)                                                                                                                                          gagGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCtt                                                                                 >  1:416308/1‑300 (MQ=255)                                                                                                                                                aTCCAGTATGCTGGCCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTACGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATATAGGGAGTAGTAGATCAGCATTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCATCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGagag                                                                           <  2:333461/300‑1 (MQ=255)                                                                                                                                                       agGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGcc                                                                     <  1:198945/299‑1 (MQ=255)                                                                                                                                                           tGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCggtgg                                                                <  1:170856/300‑1 (MQ=255)                                                                                                                                                                      atgatAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGTTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAAc                                                     <  1:217971/300‑1 (MQ=255)                                                                                                                                                                      atgatAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGTTGGTGTGGATAAAc                                                     <  1:217876/300‑1 (MQ=255)                                                                                                                                                                      atgatAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAAc                                                     <  1:567747/300‑1 (MQ=255)                                                                                                                                                                       tgatAAGCACCATCAGTGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACa                                                    <  1:451038/300‑1 (MQ=255)                                                                                                                                                                       tgatAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACa                                                    <  1:451120/300‑1 (MQ=255)                                                                                                                                                                        gatAAGCATCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCAAAGGGTGGAGCCGATCCCGCATGCGCCGACCATGCAGACGACGGTTGCCGAGCGGACGTTGGATTTGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTCGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTACACCGGGACGGGCTCAATCGTTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACa                                                    >  2:243250/1‑299 (MQ=255)                                                                                                                                                                        gatAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCAACTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAACAGCTTGGAGAGCATGCCGGTGGTGTGGATAAACa                                                    >  2:93118/1‑299 (MQ=255)                                                                                                                                                                               aCCATCAGGCTGCAGGTTTGTTGGAGCTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACTACGGTTGCCGAGCGGACGTTGGATTCTAAGCGATAGAGAGAGTAGTAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACa                                                    <  2:204675/292‑1 (MQ=255)                                                                                                                                                                                             ggTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCAAAGGGTGACGCCGACCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCTAAGCGATAGAGGGAGTAGTAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCTTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGTTGGTGTGGATAAACAGCGCCagcacgccagcacgcc                               <  2:41347/299‑1 (MQ=255)                                                                                                                                                                                                            gAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGggccgagg               <  2:583891/300‑1 (MQ=255)                                                                                                                                                                                                                         gCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCa  >  1:442576/1‑300 (MQ=255)                                                                                                                                                                                                                                             gATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAg                                                   >  2:485517/1‑231 (MQ=255)                                                                                                                                                                                                                                             gATCCCGCCTGCGCCGACCATGCCGACGACGGTTGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAg                                                   <  1:485517/231‑1 (MQ=255)                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                       GTAGTGTTGACGAAGCTCCTGCTCAAGTTTTGCGGCTATCTCTTGATAGCGTGTTGGGTAGCTGGTCGGATGTGTAGACAAGTGCATAGATATCAATGCCTCGCTTATCAGATAAAGTGCTTACGCAACCGTTGAGAGAGGAAATCCAGCAGGCTGACCGTGACGATGATAAGCACCATCAGGGCGCAGGTTTGTTGGAACTGGAAACCGCGAATCGCTTCCCACAGGGTGACGCCGATCCCGCCTGCGCCGACCATGCCGACGACGGTCGCCGAGCGGACGTTGGATTCGAAGCGATAGAGGGAGTAGGAGATCAGCAGTGGCATCACCTGTGGCAGCACGCCGTAGAGGATCTCTTCGAGCTTGTTGGCACCGGTGGCGCGAATGCCTTCCACCGGGCCGGGCTCAATCGCTTCCACCGCTTCGGAAAGCAGCTTGGAGAGCACGCCGGTGGTGTGGATAAACAGCGCCAGCACGCCAGCACGCCAGCGAACGGGCCGAGGCCGACGGCGACCA  >  NC_000913/4322336‑4322851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑