Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,320,079	C→T	Q195Q (CAG→CAA)	phnI ←	carbon‑phosphorus lyase core complex subunit PhnI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,320,079	0	C	T	100.0%	176.0 / NA	51	Q195Q (CAG→CAA)	phnI	carbon‑phosphorus lyase core complex subunit PhnI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/27);  total (24/27)

AGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGACGCATCCCGG  >  NC_000913/4319798‑4320374                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                        agtgccatcgccatcGCTTTGCGCTCGCTCAGGCCGAACACCAGCCCTTGGTCGCGCGTGAAGTGCTGCGGCTCACCTGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGgaggag                                                                                                                                                                                                                                                                                       <  2:100739/297‑1 (MQ=255)        tcgccatcgcTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGTGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCTACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACTTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGAGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGc                                                                                                                                                                                                                                                                                <  1:287211/300‑1 (MQ=255)        tcgccatcgcTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGc                                                                                                                                                                                                                                                                                <  1:418183/300‑1 (MQ=255)         cgccatcgcTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGCCAAACCCGTTCACCACTTCAAACTCAATAATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTACGGCACAATCGACCCGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATTAATTGCTGCAAACGGGAGGAGCGTGAgta                                                                                                                                                                                                                                                                               >  2:698686/1‑298 (MQ=255)                                                      gcgcgTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTgcgc                                                                                                                                                                                                                                   <  1:298352/299‑1 (MQ=255)                                                       cgcgTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGcgcc                                                                                                                                                                                                                                  >  1:633012/1‑299 (MQ=255)                                                         cgTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCANCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCt                                                                                                                                                                                                                               >  1:150354/1‑300 (MQ=255)                                                             aaGTGCGGCGGCTCACCCGGCGGTTCGATAAAACCGTTAACCATTTCACACTCATTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCATGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATcc                                                                                                                                                                                                                           <  2:386251/300‑1 (MQ=255)                                                             aaGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATcc                                                                                                                                                                                                                           >  1:499782/1‑300 (MQ=255)                                                               gTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCctc                                                                                                                                                                                                                         >  2:702602/1‑300 (MQ=255)                                                                      ggcTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAAc                                                                                                                                                                                                                  <  1:280044/300‑1 (MQ=255)                                                                                        aTAAAACCGTTAACCATTCCACACTCAGTCATCAGCAGTTCGCCGACGTCCACCGCAAAGCCCAGCTCTTCCGGCACAATCGACACGTCGATACAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTTGGTGGAGTAGGCCAGCACCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCGGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGc                                                                                                                                                                                                                                          <  2:198078/258‑1 (MQ=255)                                                                                                     ccATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGTGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGa                                                                                                                                                                                   >  1:208715/1‑300 (MQ=255)                                                                                                       gtttCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGACAGCGCCAGCAAATAGCCTTCGTCGAAGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTACACCGGCGGCGTGCGGGTGATGTCATCCGGCCGTGCGCCGCTATCCTCTTCAAACTTCGAAAGCCCATGACGCGCCAGCAGGCTGaaa                                                                                                                                                                                 >  2:245050/2‑300 (MQ=255)                                                                                                       aTTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCCAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGaaa                                                                                                                                                                                 >  1:721066/1‑300 (MQ=255)                                                                                                                 cAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTgcggc                                                                                                                                                                        >  2:710054/1‑299 (MQ=255)                                                                                                                   gTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCACTGACGCGCCAGCAGGCTGAAAACGTGCGGCGAc                                                                                                                                                                     >  2:302749/1‑300 (MQ=255)                                                                                                                                  ccGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCt                                                                                                                                                       >  2:610621/1‑299 (MQ=255)                                                                                                                                  ccGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCATTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCt                                                                                                                                                       >  2:610614/1‑299 (MQ=255)                                                                                                                                    gACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCTGCGACGGCTGCTGTTCGCTg                                                                                                                                                      <  1:9265/298‑1 (MQ=255)                                                                                                                                    gACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTg                                                                                                                                                      <  1:9463/298‑1 (MQ=255)                                                                                                                                              gCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTc                                                                                                                                          <  1:273609/300‑1 (MQ=255)                                                                                                                                                                             cGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTcggcgg                                                                                                                                               >  2:178249/1‑264 (MQ=255)                                                                                                                                                                             cGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTcggcgg                                                                                                                                               <  1:178249/264‑1 (MQ=255)                                                                                                                                                                                          gCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCa                                                                                               >  1:558601/1‑299 (MQ=255)                                                                                                                                                                                                  tctcGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGTGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATggg                                                                                      <  2:620409/300‑1 (MQ=255)                                                                                                                                                                                                         tGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATGGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTNAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCNGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAACAGGCGATGGGTGTAGTc                                                                               <  2:546897/300‑1 (MQ=255)                                                                                                                                                                                                         tGCGAACGGGTGAGTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGACGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGt                                                                                <  2:53291/299‑1 (MQ=255)                                                                                                                                                                                                         tGCGAACGGGGGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGTAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGt                                                                                <  2:53292/299‑1 (MQ=255)                                                                                                                                                                                                                gggTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCTGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCATCCCCTGACGCGCCAGCAGGCTGAAAACGTGCTGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGt                                                                         <  2:665170/299‑1 (MQ=255)                                                                                                                                                                                                                gggTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGt                                                                         <  2:575964/299‑1 (MQ=255)                                                                                                                                                                                                                 ggTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTTGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGt                                                                         <  1:242055/298‑1 (MQ=255)                                                                                                                                                                                                                      ttGCGCCCGTAACCGCGTTGGTTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCa                                                                  <  2:689328/300‑1 (MQ=255)                                                                                                                                                                                                                          gcCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAagca                                                              <  1:200534/300‑1 (MQ=255)                                                                                                                                                                                                                                         gggTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCACACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGaa                                               >  1:208442/1‑300 (MQ=255)                                                                                                                                                                                                                                         gggTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGaa                                               >  1:709644/1‑300 (MQ=255)                                                                                                                                                                                                                                         gggTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGa                                                >  2:409651/1‑299 (MQ=255)                                                                                                                                                                                                                                         gggTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCAAGCAGGGTAAAATCGAGCAGGCGATGGATGTAGTCGTAGGTTGGGCCCAGCAGCTGGCCGCCGGGaa                                               >  2:85248/1‑300 (MQ=255)                                                                                                                                                                                                                                         gggTGGAGCAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGTATCACTTGCTGCAACCGGGAGGAGCGTGAGCAGGGGTAACCCGACGGCGTGCGGGTGATGTCATCCGGCTGTGCGCAGCTATCCTATTCAAACTTCGCAAGCCCATGACGCGCCAGCAGGCTGAAAACATGCGGCGACGGCTACTATTCGCTGTAGGCGGCGGTACGCGTCGGCGCTTCACCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAACTGGCCGCCGGGac                                               >  2:409657/1‑299 (MQ=255)                                                                                                                                                                                                                                              gAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCGTCAATTGCTGCAGACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGTTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCTCCAGCCCCTTACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAg                                                                                 <  2:14747/261‑1 (MQ=255)                                                                                                                                                                                                                                              gAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGTAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAg                                                                                 <  2:14357/261‑1 (MQ=255)                                                                                                                                                                                                                                              gAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAANGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGACGGGAATGTCt                                          >  2:221109/1‑300 (MQ=255)                                                                                                                                                                                                                                              gAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAg                                                                                 >  1:14357/1‑261 (MQ=255)                                                                                                                                                                                                                                              gAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCt                                          >  1:214770/1‑300 (MQ=255)                                                                                                                                                                                                                                              gAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCAAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAg                                                                                 >  1:14747/1‑261 (MQ=255)                                                                                                                                                                                                                                                               cAAATAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGAt                         >  1:683704/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   tAGCCTTCGTCGCCGCGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACgg                     <  1:471341/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              gccgcGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGTTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGAc           <  1:208568/299‑1 (MQ=255)                                                                                                                                                                                                                                                                              gccgcGCATCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGAc           <  1:172894/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                      tCAATTGCTGCAAACGGTAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGTTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCATTCGAGACGCATCCCgg  <  2:499782/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      tCAATTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGACGCATACCgg  <  1:702602/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                        AGCGCCATCGCCATCGCTTTGCGCTCGCTCATGCCGAACACCAGCCCGTAGCCGCGCGTGAAGTGCGGCGGCTCACCCGGCGGGTCGATAAAACCGTTAACCATTTCACACTCAGTCATCAGCAGTTCGCCGACGTTCACCGCAAATCCCAGCTCTTCCGGCACAATCGACACGTCGATATAGCCGCTGCGGATCTCGCCTGCGAACGGGTGATTGCGCCCGTAACCGCGTTGGGTGGAGTAGGCCAGCGCCAGCAAATAGCCTTCGTCGCCGCGCATCAACTGCTGCAAACGGGAGGAGCGTGAGCAGGGGTAAACCGGCGGCGTGCGGGTGATGTCATCCGGCTGTGCGCCGCTATCCTCTTCAAACTTCGCCAGCCCCTGACGCGCCAGCAGGCTGAAAACGTGCGGCGACGGCTGCTGTTCGCTGTCGGCGGTGGTCAGCGTCGGCGCTTCGCCGTTTGCCAGCAGGGTAAAATCGAGCAGGCGATGGGTGTAGTCGTAGGTTGGGCCAAGCAGCTGGCCGCCGGGAATGTCTTTATAAACGGCGGAGATACGGCGTTCGAGACGCATCCCGG  >  NC_000913/4319798‑4320374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑