Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,286,033	C→T	A447T (GCG→ACG)	acs ←	acetyl‑CoA synthetase (AMP‑forming)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,286,033	0	C	T	100.0%	203.3 / NA	61	A447T (GCG→ACG)	acs	acetyl‑CoA synthetase (AMP‑forming)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/31);  total (30/31)

GCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACC  >  NC_000913/4285738‑4286322                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                   gcTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGt                                                                                                                                                                                                                                                                                                <  1:628821/300‑1 (MQ=255)       gcagtCTTCGGATGCGCCACTAGCGCCGACTCAATCTCTGCCGTCCCCAGACGCGGACCTGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCATTAATAGCCATTTTCATCGCGACGCGCGCCGTCGCCGCTTAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCTTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGATTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGt                                                                                                                                                                                                                                                                                                <  2:185167/290‑1 (MQ=255)         aaTCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGTAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGc                                                                                                                                                                                                                                                                                         <  1:286242/299‑1 (MQ=255)         aaTCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGc                                                                                                                                                                                                                                                                                         <  1:286695/299‑1 (MQ=255)                                    cTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCATGAACAGGCAAGGAGTCGGTGATTACCAGGCTACCTTCGGNGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGc                                                                                                                                                                                                                                                             >  1:144404/1‑300 (MQ=255)                                        aTCTCTGCTGTCCCCAGACGGGGACCGTAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTTAAGGTGTAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTc                                                                                                                                                                                                                                                         <  2:96760/300‑1 (MQ=255)                                        aTCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCNCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTc                                                                                                                                                                                                                                                         >  1:150545/1‑300 (MQ=255)                                           tctaccGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGTTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGNGGACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGc                                                                                                                                                                                                                                                      <  2:514718/296‑1 (MQ=255)                                           tctGCCGTCCCCATGCGGTGACCGTAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGNGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGc                                                                                                                                                                                                                                                      <  2:514733/300‑1 (MQ=255)                                           tctGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGTAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGc                                                                                                                                                                                                                                                      <  1:402436/300‑1 (MQ=255)                                                       aGACGGTGACCTTAGACGTTCAGCACGTCGTCCACACGCCCGGTGATCCAGGAATAGCCATCTTCAGCGCGACGCGCGTCGTCGCCGCTGAAATACATATTTTTGAAGGGGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑a                                                                                                                                                                                                                                         <  2:343622/300‑1 (MQ=255)                                                       aGACGGTGACCGTAGACGTGCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑a                                                                                                                                                                                                                                         <  2:343612/300‑1 (MQ=255)                                                              gACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGc                                                                                                                                                                                                                                   >  1:397263/1‑299 (MQ=255)                                                                    agaCGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGTAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGa                                                                                                                                                                                                                            <  2:287265/300‑1 (MQ=255)                                                                        gTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATCCATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTCTCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCAt                                                                                                                                                                                                                        >  2:717119/1‑300 (MQ=255)                                                                        gTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCAt                                                                                                                                                                                                                        >  1:405218/1‑300 (MQ=255)                                                                          tCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGACGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGa                                                                                                                                                                                                                      >  2:347952/1‑300 (MQ=255)                                                                            aGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGTAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGaa                                                                                                                                                                                                                     <  1:265107/299‑1 (MQ=255)                                                                            aGCACGGCGTCCACACGCCCGGTGATCCAGTAATAGCCATCTGCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGaa                                                                                                                                                                                                                     <  2:490508/299‑1 (MQ=255)                                                                                 gtcgtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAAccgcc                                                                                                                                                                                                               >  1:464315/1‑300 (MQ=255)                                                                                 gtagtcCACACGCACGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGAACCGGCCAGGAGCCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAAc                                                                                                                                                                                                                                                                                 >  2:659188/4‑235 (MQ=255)                                                                                   cgtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAAccgcc                                                                                                                                                                                                               >  1:255686/1‑298 (MQ=255)                                                                                    gtcCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCAAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCANCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGt                                                                                                                                                                                                            >  2:555139/1‑300 (MQ=255)                                                                                    gtcCACACGCCCGGTTATCCAGTAATAGCCATCTCCATCGCGACGCGCGCCGTCGCCGCTGAAATACACATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGTGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGCAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCCGCGGCAGCGGGGTGATCATGAAAccgcc                                                                                                                                                                                                               >  2:239683/1‑298 (MQ=255)                                                                                                 gTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGcca                                                                                                                                                                                                >  2:171872/1‑299 (MQ=255)                                                                                                 gTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAAACGGCTTTCAGCTAGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGccac                                                                                                                                                                                               >  2:171626/1‑300 (MQ=255)                                                                                                     tCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAgg                                                                                                                                                                                           <  1:296363/300‑1 (MQ=255)                                                                                                        aGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTAt                                                                                                                                                                                        <  1:176355/300‑1 (MQ=255)                                                                                                         gTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATc                                                                                                                                                                                       >  1:35069/1‑300 (MQ=255)                                                                                                         gTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGACGGTTTCGGTCTGCCACCAGGTATc                                                                                                                                                                                       >  1:587737/1‑300 (MQ=255)                                                                                                            aTAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGTTGGAGAAGTAGGTCTGTTCAAAACGTGCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGac                                                                                                                                                                                    <  2:718193/300‑1 (MQ=255)                                                                                                            aTAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGac                                                                                                                                                                                    <  1:561/300‑1 (MQ=255)                                                                                                                        aTCGCGACGCGCGCCGTCGCCGCTTAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACAtt                                                                                                                                                                         <  2:335434/299‑1 (MQ=255)                                                                                                                        aTCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACAttt                                                                                                                                                                        >  1:416982/1‑300 (MQ=255)                                                                                                                        aTCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACAtt                                                                                                                                                                         <  1:555139/299‑1 (MQ=255)                                                                                                                        aTCGCGACGCACGCCGTCGCCGCTGAAATACATATTTTTGAAGTTGTAGAGGTAGGTCTGTTCAAAACGTGCGTGATCGCCAAACAGCGTACGCGCCTTACCCTGCCATGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCTAGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTGTCGACCACCGGACAtt                                                                                                                                                                         <  2:335466/299‑1 (MQ=255)                                                                                                                                                 aaaTACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGTCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTAc                                                                                                                                               <  2:604246/300‑1 (MQ=255)                                                                                                                                                       atatTTTTGAAGGTTGAGAAGTAGGTTTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGNTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTc                                                                                                                                          <  2:237200/299‑1 (MQ=255)                                                                                                                                                       atatTTTTGAAGGTGTAGAAGTAGGTCTGTTCAAAACGGTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGTTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTc                                                                                                                                          <  2:204733/299‑1 (MQ=255)                                                                                                                                                       atatTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTc                                                                                                                                          <  1:453702/299‑1 (MQ=255)                                                                                                                                                                                   gTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCCCGCTTCCGGGTTAATTGGCTCGccc                                                                                                              >  1:699691/1‑299 (MQ=255)                                                                                                                                                                                   gTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCa                                                                                                             >  1:176523/1‑300 (MQ=255)                                                                                                                                                                                                    tCGCCAAATAGCGTACGCACCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACTACTCCCACGc                                                                                                                                    >  2:103948/1‑260 (MQ=255)                                                                                                                                                                                                    tCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATgcgc                                                                                            >  1:279771/1‑300 (MQ=255)                                                                                                                                                                                                      gCCAAACAGCGGACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCATGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATgcgc                                                                                            <  2:296891/298‑1 (MQ=255)                                                                                                                                                                                                                     cgcCTGACCCGGCCAGGAGTCGGTGATTACAAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTACAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATACGCAGCGAAGAAtggtaggt                                                                           >  2:386165/1‑300 (MQ=255)                                                                                                                                                                                                                               ggCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCg                                                                    <  1:364876/297‑1 (MQ=255)                                                                                                                                                                                                                               ggCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATc                                                                 >  1:62990/1‑300 (MQ=255)                                                                                                                                                                                                                                gCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATc                                                                 >  1:475837/1‑299 (MQ=255)                                                                                                                                                                                                                                          ggTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGANGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGc                                                       <  1:509565/299‑1 (MQ=255)                                                                                                                                                                                                                                                         aCCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTACGCCATCAgcgc                                       >  2:653057/1‑300 (MQ=255)                                                                                                                                                                                                                                                             tCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAgcgcgc                                     >  1:109093/1‑298 (MQ=255)                                                                                                                                                                                                                                                                       ccTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTNGGTCTGCCACCAGGTATCGACCACCGGACATTNCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGAt                                                                  <  2:537861/259‑1 (MQ=255)                                                                                                                                                                                                                                                                       ccTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGAt                                                                  >  1:537861/1‑259 (MQ=255)                                                                                                                                                                                                                                                                        cTCCAGCGGGTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGAACACCGGACATTTCTCGTTGCCGATTTTTTTCCCGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGt                           >  1:94634/1‑297 (MQ=255)                                                                                                                                                                                                                                                                               gggTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATNCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGta                 >  1:155754/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                ggTTACCTTCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGtat                >  1:290910/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                       tttgTTATCGACCATCTTCGTTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAat         <  2:150545/297‑1 (MQ=255)                                                                                                                                                                                                                                                                                       ttCGTTATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑ANGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAgaga           <  1:144587/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                           ttATCGACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGANCGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATAtt      >  1:135566/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                gACCAGCGTCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCTGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCATCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGAcc  <  2:280893/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                   GCTTCGGCAATCTTCGGATGCGCCACCAGCGCCGACTCAATCTCTGCCGTCCCCAGACGGTGACCGGAGACGTTCAGCACGTCGTCCACACGCCCGGTTATCCAGTAATAGCCATCTTCATCGCGACGCGCGCCGTCGCCGCTGAAATACATATTTTTGAAGGTGGAGAAGTAGGTCTGTTCAAAACGTTCGTGATCGCCAAACAGCGTACGCGCCTGACCCGGCCAGGAGTCGGTGATTACCAGGCTACCTTCGGTGGCCCCCTCCAGCGGGTTACCTTCGTTATCGACCAGCGCCGGTTGCACGCCGAAGAACGGACGTGTTGCCGAACCGGCTTTCAGCTCGGTAGCGCC‑AGGCAGCGGGGTGATCATGAAACCGCCGGTTTCGGTCTGCCACCAGGTATCGACCACCGGACATTTCTCGTTGCCGATTTTTTTCCAGTACCACTCCCACGCTTCCGGGTTAATTGGCTCGCCCACGGAACCGAGAATGCGCAGCGACGAACGGTCGGTGCCTTCGATCGCTTTATCGCCTTCCGCCATCAGCGCGCGGATCGCCGTGGGTGCGGTATAGAGAATATTGACC  >  NC_000913/4285738‑4286322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑