Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,179,001	C→T	S41S (AGC→AGT)	rplA →	50S ribosomal subunit protein L1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,179,001	0	C	T	98.7%	263.2 / NA	74	S41S (AGC→AGT)	rplA	50S ribosomal subunit protein L1
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (34/39);  total (35/39)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGCGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACC  >  NC_000913/4178707‑4179296                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                         gTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGNAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGa                                                                                                                                                                                                                                                                                                     >  1:151322/1‑300 (MQ=255)  tCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCTACATGACTTGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTTAAAGAGCTGGCGACTGCTAAATTCTTAGAAAGTGTGGAc                                                                                                                                                                                                                                                                                                    <  2:381474/300‑1 (MQ=255)    tGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACGAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACg                                                                                                                                                                                                                                                                                                   <  1:679554/299‑1 (MQ=255)              aaGCCGAACAAAGACAAAATGAATAAAATTTACCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCAATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACTAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGACAGAGCTGGCGACTGCTAAATTTGCAAAAAGCGTAGACGTAGCTGTTAAc                                                                                                                                                                                                                                                                                        >  2:297653/1‑300 (MQ=255)                   gAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTc                                                                                                                                                                                                                                                                                     <  1:583802/298‑1 (MQ=255)                   gAACAAAGACAAAGTGGGTAAAATTTCCCGCGCGCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACGGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTTGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTc                                                                                                                                                                                                                                                                                     <  1:583832/298‑1 (MQ=255)                          gacaaagTGGGTAAAATTTCCAGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGAc                                                                                                                                                                                                                                                                            >  1:199547/1‑300 (MQ=255)                           acaaagTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACg                                                                                                                                                                                                                                                                           <  1:586122/300‑1 (MQ=255)                                     tAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATc                                                                                                                                                                                                                                                                 <  2:498493/300‑1 (MQ=255)                                     tAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATc                                                                                                                                                                                                                                                                 <  1:463798/300‑1 (MQ=255)                                                gcgcTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAGGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTTACCAGAac                                                                                                                                                                                                                                                       >  2:9716/1‑299 (MQ=255)                                                gcgcTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAac                                                                                                                                                                                                                                                       <  2:591542/299‑1 (MQ=255)                                                    tCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTTAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGGCCAGAacgtacg                                                                                                                                                                                                                                                  <  2:160341/300‑1 (MQ=255)                                                    tCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGANGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAacgtacg                                                                                                                                                                                                                                                  <  1:137944/300‑1 (MQ=255)                                                    tCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGANGGTACTGCA‑CGTTACATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAacgtacg                                                                                                                                                                                                                                                  <  1:157350/300‑1 (MQ=255)                                                    tCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAacgtacg                                                                                                                                                                                                                                                  <  1:397212/300‑1 (MQ=255)                                                    tCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAAACTCGGCATCGACGCTCGTAAATCTGACCAGAacgtacg                                                                                                                                                                                                                                                  <  1:724485/300‑1 (MQ=255)                                                             ggAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAAc                                                                                                                                                                                                                                         <  1:117660/300‑1 (MQ=255)                                                               aaaTCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CNTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTg                                                                                                                                                                                                                                       <  1:156884/300‑1 (MQ=255)                                                               aaaTCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATGTAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CNTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTg                                                                                                                                                                                                                                       <  1:156775/300‑1 (MQ=255)                                                                           ccAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCt                                                                                                                                                                                                                                                                         >  2:501052/1‑254 (MQ=255)                                                                           ccAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCt                                                                                                                                                                                                                                                                         <  1:501052/254‑1 (MQ=255)                                                                                     cGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGATGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGcc                                                                                                                                                                                                                  >  2:201091/1‑299 (MQ=255)                                                                                      gACATTACTGTTGCCGACATTGAATCGATTACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGTTAGT‑GGAGGAC‑TAATAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCTCTAGAAAGTTGATGCAACCAAACAGTACGACATCGACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCTTAGAAAGTGTGGACTTAGCTGTTAATCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTATTGCCGCACGGTACTGGcc                                                                                                                                                                                                                  <  2:165180/298‑1 (MQ=255)                                                                                      gACATGACTGGTGCCGACATTTAAGCGATTACTCGCTCCATCGAAGGTACTACA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGcc                                                                                                                                                                                                                  <  2:324450/298‑1 (MQ=255)                                                                                      gACATGACTGGTGCCGACATTTAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGcc                                                                                                                                                                                                                  <  2:497417/298‑1 (MQ=255)                                                                                      gACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGcc                                                                                                                                                                                                                  <  1:46382/298‑1 (MQ=255)                                                                                      gACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGAAATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGcc                                                                                                                                                                                                                  <  1:452417/298‑1 (MQ=255)                                                                                      gACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCAATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGcc                                                                                                                                                                                                                  <  1:287888/298‑1 (MQ=255)                                                                                           gACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGccgttccgt                                                                                                                                                                                                           >  1:644026/1‑300 (MQ=255)                                                                                              tGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCcgttccgttc                                                                                                                                                                                                         <  1:461804/299‑1 (MQ=255)                                                                                                           gAAGCGATGACTCGCTCCATCGAAGTGACTACA‑CGTTCCAGGGGACTGGGAGT‑GTAGGAC‑GAAGAAATGGTTAAACTGACCAAGCGCATGNGTGTGATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTTAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACTTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTTACCAGAACGTACTTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTactc                                                                                                                                                                                           <  2:563388/300‑4 (MQ=255)                                                                                                            aagctacgACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGNGTGTTATCCGCTAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAt‑tt                                                                                                                                                                                          <  2:563379/292‑1 (MQ=255)                                                                                                            aaGCAATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGccctgtctcttatacacatctcgcag                                                                                                                                                                                          <  1:403990/300‑25 (MQ=255)                                                                                                                gATGCCTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTAccc                                                                                                                                                                                      <  2:52633/299‑1 (MQ=255)                                                                                                                   gACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATNTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCaa                                                                                                                                                                                    >  1:153501/1‑298 (MQ=255)                                                                                                                             aTCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GTAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCt                                                                                                                                                                        >  1:682768/1‑300 (MQ=255)                                                                                                                             aTCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCt                                                                                                                                                                        >  1:669141/1‑300 (MQ=255)                                                                                                                               gcgagggaCTGCAACNTTCCATGGGCCTGGTAGTNGGAGGACCTAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTATNTTACCCAAGGTGCaaa                                                                                                                                                                            <  1:599834/291‑1 (MQ=255)                                                                                                                                 aaGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGaa                                                                                                                                                                     >  2:606395/1‑299 (MQ=255)                                                                                                                                  aGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GTAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCTAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAgc                                                                                                                                                                   <  2:451699/300‑1 (MQ=255)                                                                                                                                   ggTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAgct                                                                                                                                                                  >  1:441791/1‑300 (MQ=255)                                                                                                                                    gTACTGCC‑CGCTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAGTGGTTAAACTGACCAAGCGCATGCGTATTATCCGCGAGAAAGTTGATGCAACCACACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAGAGTGTGGACGCAGCTGTTAACCTCGGCACCGAAGCTCGTAAACCTGAACAAAACGTCAGTGGTGCAACTGTACCGCCGCACGGTACTGGACGGTCCATCCGCGTAGc                                                                                                                                                                                                  >  2:352790/1‑268 (MQ=255)                                                                                                                                    gTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAGGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGACGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATTTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGACGTAT‑TTACCCAAAGTGTAAACGCTAAAgct                                                                                                                                                                  >  2:353115/1‑299 (MQ=255)                                                                                                                                             cGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCTTCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTCCCCAAGGTGCAAACGCTGAAGCTGCTAAAGCt                                                                                                                                                         >  2:129360/1‑300 (MQ=255)                                                                                                                                               ttCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGc                                                                                                                                                       >  2:451965/1‑300 (MQ=255)                                                                                                                                                      ggCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCa                                                                                                                                                >  2:505832/1‑300 (MQ=255)                                                                                                                                                       gCCTGGTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCa                                                                                                                                                >  1:20272/1‑299 (MQ=255)                                                                                                                                                            gTAGT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACt                                                                                                                                           >  2:586030/1‑299 (MQ=255)                                                                                                                                                               gT‑GGAGGAC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTggta                                                                                                                                       <  1:570883/300‑1 (MQ=255)                                                                                                                                                                     ggaC‑TAAGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTAt                                                                                                                                  >  2:692328/1‑300 (MQ=255)                                                                                                                                                                           aaGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCTAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAg                                                                                                                             <  2:613015/300‑1 (MQ=255)                                                                                                                                                                           aaGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAg                                                                                                                             <  2:609171/300‑1 (MQ=255)                                                                                                                                                                           aaGAAATGGTTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACACAAGGTGCAAACGCTGAAGCTGCTAACGCTGCAGGCGCAGAACTGGTAGGTATGGaa                                                                                                                              >  2:348107/1‑299 (MQ=255)                                                                                                                                                                                           gACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGAt                                                                                                             >  1:394272/1‑300 (MQ=255)                                                                                                                                                                                                    cATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGaaa                                                                                                      >  1:98498/1‑298 (MQ=255)                                                                                                                                                                                                       gCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGa                                                                                                 >  1:461839/1‑300 (MQ=255)                                                                                                                                                                                                       gCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCAAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGa                                                                                                 >  1:461580/1‑300 (MQ=255)                                                                                                                                                                                                                   cgAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACGTTGa                                                                                     >  2:417249/1‑300 (MQ=255)                                                                                                                                                                                                                                gCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCt                                                                        >  1:236630/1‑300 (MQ=255)                                                                                                                                                                                                                                 cAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCtt                                                                       >  1:116963/1‑300 (MQ=255)                                                                                                                                                                                                                                           gTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGc                                                             <  1:410831/300‑1 (MQ=255)                                                                                                                                                                                                                                                    cAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCgt                                                    >  1:302985/1‑300 (MQ=255)                                                                                                                                                                                                                                                    cAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCAGGATGCAATGCGCgt                                                    >  1:303644/1‑300 (MQ=255)                                                                                                                                                                                                                                                             tattgcatggctgAAAGAGCTGGCGACTGCTAAATTCTTAGAAAGTGTGGACGGAGCTGTTAACCGCGGCATCTACGCTCGTAAATCTGNCCAGAACGTACGTGGTGCAACTGTACTTCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAAGTGCAAATGCTGAAGCTGCTAAAGCGGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGTCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGcc                                            <  2:558086/290‑1 (MQ=255)                                                                                                                                                                                                                                                             tATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGNCCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGcc                                            <  2:558191/299‑1 (MQ=255)                                                                                                                                                                                                                                                             tATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTggac                                            <  1:714993/299‑3 (MQ=255)                                                                                                                                                                                                                                                             tATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGcc                                            <  1:113137/299‑1 (MQ=255)                                                                                                                                                                                                                                                                      gctgAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTACCCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACAAGATCAAGAAAGGCGAAATGAAATTTGACGTTGTTATTGTTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGcc                                   >  2:52432/1‑299 (MQ=255)                                                                                                                                                                                                                                                                      gctgAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGAACAGATCAAGAAAGGCGAAATGAACCTTGAAGTTGTTATTGCTTCTACGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCa                                  >  2:337161/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      gctgAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTAGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTACGGATGCAATGTGCGTTGTTGGCCAGCTGGGc                                    >  2:7501/1‑298 (MQ=255)                                                                                                                                                                                                                                                                         gAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCAGGt                               >  1:290957/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             gagCTGGCGACTGCTAAATTCGTAGAAAGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCNTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCAGGTTCt                            >  2:517941/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                      aaaGTGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAAcc  <  2:116963/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                         GTCTGGTTCCGGTAAGCCGAACAAAGACAAAGTGGGTAAAATTTCCCGCGCTCAGCTGCAGGAAATCGCGCAGACCAAAGCTGCCGACATGACTGGTGCCGACATTGAAGCGATGACTCGCTCCATCGAAGGTACTGCA‑CGTTCCATGGGCCTGGTAGT‑GGAGGAC‑TAAGAAATGGCTAAACTGACCAAGCGCATGCGTGTTATCCGCGAGAAAGTTGATGCAACCAAACAGTACGACATCAACGAAGCTATCGCACTGCTGAAAGAGCTGGCGACTGCTAAATTCGTAGAAAGCGTGGACGTAGCTGTTAACCTCGGCATCGACGCTCGTAAATCTGACCAGAACGTACGTGGTGCAACTGTACTGCCGCACGGTACTGGCCGTTCCGTTCGCGTAGCCGTAT‑TTACCCAAGGTGCAAACGCTGAAGCTGCTAAAGCTGCAGGCGCAGAACTGGTAGGTATGGAAGATCTGGCTGACCAGATCAAGAAAGGCGAAATGAACTTTGACGTTGTTATTGCTTCTCCGGATGCAATGCGCGTTGTTGGCCAGCTGGGCCAGGTTCTGGGTCCGCGCGGCCTGATGCCAAACC  >  NC_000913/4178707‑4179296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑