BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,581,316	G→A	G60E (GGA→GAA)	yhhY →	N‑acetyltransferase YhhY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,581,316	0	G	A	98.8%	282.5 / NA	81	G60E (GGA→GAA)	yhhY	N‑acetyltransferase YhhY
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (45/35); minor base C (1/0); total (46/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGGAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGACTGGTAAGAAGTACGCATTGCGTAATGGTGAATATGTCGATGCATATTATAT  >  NC_000913/3581023‑3581610

ataataatCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGAcgt                                                                                                                                                                                                                                                                                                   >  1:234555/1‑300 (MQ=255)

ataataatCATTCTCANTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGNCATCAAGCAACTCGTCGCNTGTATTGATGAAGAcgt                                                                                                                                                                                                                                                                                                   >  1:601240/1‑300 (MQ=255)

       tcattctcattcGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGc                                                                                                                                                                                                                                                                                            <  1:699949/300‑1 (MQ=255)

               attcGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGTAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCAc                                                                                                                                                                                                                                                                                    <  1:350809/300‑1 (MQ=255)

                   gCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCAt                                                                                                                                                                                                                                                                                 <  2:678918/299‑1 (MQ=255)

                    cACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCAtt                                                                                                                                                                                                                                                                                >  1:684170/1‑299 (MQ=255)

                    cACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCATGTATTGATGAAGACGTCGTGGGCCATCTCACCAtt                                                                                                                                                                                                                                                                                >  1:115315/1‑299 (MQ=255)

                     aCTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATGTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAAGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCGGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGa                                                                                                                                                                                                                                                                              <  2:485103/300‑1 (MQ=255)

                       ttGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACg                                                                                                                                                                                                                                                                            <  1:382602/300‑1 (MQ=255)

                         gTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGt                                                                                                                                                                                                                                                                           >  1:103908/1‑299 (MQ=255)

                         gTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCAGGCATCAAGCAACTCGTCGCATGTATTGATGAAGACGTCGTGGGCCATCTCACCAttaaagt                                                                                                                                                                                                                                                                           >  2:41918/1‑294 (MQ=255)

                             aaCACTTTTTGCAAAAAAAGGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGTACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAAc                                                                                                                                                                                                                                                                      <  2:689600/300‑1 (MQ=255)

                             aaCACTTTTGGCAAAGAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAATGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGTAGGTGC‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAACGACTCGCCGATCGTCCCTGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAAc                                                                                                                                                                                                                                                                      <  2:493541/300‑1 (MQ=255)

                                               aTGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTTAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTc                                                                                                                                                                                                                                                    <  2:342422/300‑1 (MQ=255)

                                                             cATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGAtttt                                                                                                                                                                                                                                      >  1:372620/1‑300 (MQ=255)

                                                             cATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTAACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGAtttt                                                                                                                                                                                                                                      >  1:163882/1‑300 (MQ=255)

                                                                     aGTCGATGAGGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACTGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGTAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCTCAGTCATGTTGCCGATTTTGGTATCtg                                                                                                                                                                                                                              <  2:63024/300‑1 (MQ=255)

                                                                               gTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGNCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCAGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGActct                                                                                                                                                                                                                    >  2:513088/1‑300 (MQ=255)

                                                                               gTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGActct                                                                                                                                                                                                                    >  1:186219/1‑300 (MQ=255)

                                                                                      gggACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGga                                                                                                                                                                                                             >  2:417160/1‑300 (MQ=255)

                                                                                       ggACATTTACCCCAAAGAGTACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGTAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGga                                                                                                                                                                                                             <  2:6500/299‑1 (MQ=255)

                                                                                        gACATTTACCCCAAAGAGGACAAAGGAGTGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAAAACACTACAGGTGCCTCATCCTTCCGATCATCTGTGGCAGGAGCGACTCGCCGATCGTCCAGGCATCAAGCAACTCGTCGCCTGTATTGATGAATACGTCGTGGGCCATCTCACCATTGACGt                                                                                                                                                                                                                                                                           >  2:147923/1‑236 (MQ=255)

                                                                                        gACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTACGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCATGTATTGATGAAGACGTCGTGGGCCATCTAACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGgaa                                                                                                                                                                                                            >  2:148726/1‑299 (MQ=255)

                                                                                                    aaaGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCAGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTc                                                                                                                                                                                               >  2:665747/1‑300 (MQ=255)

                                                                                                       gagGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTAAAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGc                                                                                                                                                                                             >  1:23226/1‑299 (MQ=255)

                                                                                                          gACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAgc                                                                                                                                                                                         <  2:656704/300‑1 (MQ=255)

                                                                                                                   aTGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATg                                                                                                                                                                                <  1:663883/300‑1 (MQ=255)

                                                                                                                   aTGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCATGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTTTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATg                                                                                                                                                                                <  1:663888/300‑1 (MQ=255)

                                                                                                                     gagtgagATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGc                                                                                                                                                                               >  1:191920/1‑299 (MQ=255)

                                                                                                                          agaTAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGTAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCgagaga                                                                                                                                                                         <  1:189126/300‑1 (MQ=255)

                                                                                                                          agaTAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTATCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCgagaga                                                                                                                                                                         >  1:98781/1‑300 (MQ=255)

                                                                                                                          agaTAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTAATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCAGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGACAGCGCCCTGATGCgagaga                                                                                                                                                                         >  1:500730/1‑300 (MQ=255)

                                                                                                                               gTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCNGCATCAAGCAACTCGTCGACTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGAtt                                                                                                                                                                    >  2:517466/1‑300 (MQ=255)

                                                                                                                               gTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGAtt                                                                                                                                                                    >  1:250324/1‑300 (MQ=255)

                                                                                                                               gTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCAGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGAtt                                                                                                                                                                    >  1:250315/1‑300 (MQ=255)

                                                                                                                                                      ggATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGc                                                                                                                                             >  1:560517/1‑299 (MQ=255)

                                                                                                                                                         ttACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGCTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGt                                                                                                                                         <  2:623732/300‑1 (MQ=255)

                                                                                                                                                             gAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGAt                                                                                                                                     <  1:504527/300‑1 (MQ=255)

                                                                                                                                                              aGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTNGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATc                                                                                                                                    <  1:148726/300‑1 (MQ=255)

                                                                                                                                                              aGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTNGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATGGAAATGTGCGACAA‑CTGGTTGCGGGTAGATc                                                                                                                                    <  1:147923/300‑1 (MQ=255)

                                                                                                                                                               gccagcagggaaGATTCNCGCCCAGCCGGAGGGGTCNTGGCAACGCACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAANCTGGTTGCGGGTAGAt                                                                                                                                     <  1:599715/289‑1 (MQ=255)

                                                                                                                                                                           gATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCTCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCNCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAAc                                                                                                                       >  1:515949/1‑300 (MQ=255)

                                                                                                                                                                            aTTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCNCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAAcc                                                                                                                      <  1:530060/300‑1 (MQ=255)

                                                                                                                                                                            aTTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAAcc                                                                                                                      <  1:358208/300‑1 (MQ=255)

                                                                                                                                                                             ttCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCg                                                                                                                     <  1:470526/300‑1 (MQ=255)

                                                                                                                                                                                      aGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCANTCATGTTGCCGATTTTGGTATCTGTGTCGACTNTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGATAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGt                                                                                                              <  2:545946/298‑1 (MQ=255)

                                                                                                                                                                                           gAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCNCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAAc                                                                                                       >  1:531235/1‑300 (MQ=255)

                                                                                                                                                                                              gtgt‑ATTGCAACACACTACAGGTTCCTCATCCTTCCGATCATATGTGGCATGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGTTCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGcc                                                                                                    <  2:400380/300‑1 (MQ=255)

                                                                                                                                                                                                gt‑ATTGCAACACACTANAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGNTCGCATTGAACTAACCGTNTTTGTCGATAACGcccc                                                                                                  >  1:603540/1‑300 (MQ=255)

                                                                                                                                                                                                gt‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCAGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGccca                                                                                                  >  1:457119/1‑299 (MQ=255)

                                                                                                                                                                                                           acacTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTaa                                                                                         >  1:659587/1‑299 (MQ=255)

                                                                                                                                                                                                            cacTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTaa                                                                                         >  2:464435/1‑298 (MQ=255)

                                                                                                                                                                                                            cacTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTaa                                                                                         >  1:490696/1‑298 (MQ=255)

                                                                                                                                                                                                             acTACATGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGNAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAgg                                                                                       <  2:564238/299‑1 (MQ=255)

                                                                                                                                                                                                             acTACAGGTGCCTCATCCTTGCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGt                                                                                      <  1:351122/300‑1 (MQ=255)

                                                                                                                                                                                                              cTACAGGTGCCTCATCNTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTNACCGTGTTTGTCGATAACNCCCCGGCAATTAAGGTc                                                                                     >  1:611455/1‑300 (MQ=255)

                                                                                                                                                                                                              cTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTAAACATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCAGGCAATTAAGGTc                                                                                     >  2:646964/1‑300 (MQ=255)

                                                                                                                                                                                                              cTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCATGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAAATGTGTTTGTCGATAACGCCCCGGCAATTAAGGTc                                                                                     >  2:134809/1‑300 (MQ=255)

                                                                                                                                                                                                                                      cATATGTGGCAGGAGCGACTCGCCGATCGTCCAGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCAGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAAtt                                                             >  1:164577/1‑300 (MQ=255)

                                                                                                                                                                                                                                       atatGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAAtt                                                             >  1:462415/1‑299 (MQ=255)

                                                                                                                                                                                                                                        tatGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGCAGACGTCGTGGGCCATCTCACCATTGCCGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGGGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGCACTAACCGTGTTTGTCGATAACGCCCAGGCAATTAAGGTCTATAAAAAATAAGGCTTTGAAATTGa                                                           >  2:438389/1‑300 (MQ=255)

                                                                                                                                                                                                                                            tgGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCAGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGaa                                                          >  1:589014/1‑297 (MQ=255)

                                                                                                                                                                                                                                                         tCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTTGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCATTCATGTTGCCGATTTGGGTATCTGTTTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGATAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTGGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGACTGGTaagaag                                           <  1:392525/299‑1 (MQ=255)

                                                                                                                                                                                                                                                         tCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGACTGGTaagaag                                           <  1:663541/299‑1 (MQ=255)

                                                                                                                                                                                                                                                         tCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGACTGGTAAGAAGt                                          <  1:41918/300‑1 (MQ=255)

                                                                                                                                                                                                                                                            ccGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGACTGGTAAGAAGTAc                                        >  1:277030/1‑299 (MQ=255)

                                                                                                                                                                                                                                                            ccGATCGTCCAGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGACTGGTAAGAAGTAc                                        >  1:278457/1‑299 (MQ=255)

                                                                                                                                                                                                                                                              gATCGTCCCGGCATCAAGCAACTCGTCGCCGGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGNAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGACTGGTAAGAAGTACGc                                      <  2:234555/299‑1 (MQ=255)

                                                                                                                                                                                                                                                               aTCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGACTGGTAAGAAGTACGCAt                                    <  1:253031/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                   tCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGACTGGTAAGAAGTACGCATTGc                                 >  1:566543/1‑299 (MQ=255)

                                                                                                                                                                                                                                                                        gCATCAAGCAACTCGTCGCATGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGACAGCGCCCTGATGCGAGAGATGATTGANATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGACTGGTAAGAAGTCCGCATTGCGTAAt                            >  2:236988/1‑299 (MQ=255)

                                                                                                                                                                                                                                                                          aTCAAGCAACTCGTCGCTTGCATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGATAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGAGCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGACTGGTAAGAAGTACGCATTGCGTAATgg                          <  1:100028/299‑1 (MQ=255)

                                                                                                                                                                                                                                                                             aaGCAACTCGTCGCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGATTTGAAATTGAAGGGACTGGTAAGAAGTACGCATTGCGTAATGGTGaa                      >  2:368096/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                    tcttcgCCTGTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGANAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACNGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGACTGGTAAGAAGTACGCATTGCGTAATGGTGAATATGTc                <  2:542622/296‑1 (MQ=255)

                                                                                                                                                                                                                                                                                             gTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGACTGGTAAGAAGTACGCATTGCGTAATGGTGAATATGTCGATGCAtatt      >  2:596586/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                             gTATTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGACTGGTAAGAAGTACGCATTGCGTAATGGTGAATATGTCGATGCAtatt      >  1:472968/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                             gTATTGATGAAGACGCCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACACCGCAGTCATGTTGACAATTTTGGTATCTGTGGCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGACTGGTAAGAAGTACGCATTGCGTAATGGTGAATATGTCGATGCAtatt      <  2:186219/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                               aTTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAATGGACTGGTAAGAAGTACGCATTGCGTAATGGTGAATATGTCGATGCAtgttat    >  1:245939/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                               aTTGATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGACTGGTAAGAAGTACGCATTGCGTAATGGTGAATATGTCGATGCAtattat    <  1:665747/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                               aTTGATGAAGACGTCGTGGGCCATCTAACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAATGGACTGGTAAGAAGTACGCATTGCGTAATGGTGAATATGTCGATGCAtgttat    >  1:246221/1‑300 (MQ=255)

                                                                                                                                                                                                                                                                                                 tgatgaAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATNTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGACTGGTAAGAAGTACGCATTGCGTAATGGTGAATATGTCGATGCATATTatat  <  1:513088/300‑1 (MQ=255)

                                                                                                                                                                                                                                                                                                 ggATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTTTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGACTGGTAAGAAGTACGCATTGCGTAATGGTGAATATGTCGATGCATATTatat  <  2:684170/299‑1 (MQ=255)

                                                                                                                                                                                                                                                                                                  gATGAAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCGCGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGACTGGTAAGAAGTACGCATTGCGTAATGGTGAATATGTCGATGCATATTatat  >  1:108507/1‑299 (MQ=255)

ATAATAATCATTCTCATTCGCACTTGTCCAACACTTTTTGCAAAAAAATGCATTTGACTCGCATTTGAAAGTCAATGATGTTGAAAGGGACATTTACCCCAAAGAGGACAAAGGAATGAGTGAGATAGTAATACGCCACGCAGAAACACGGGATTACGAGGCCATCAGGCAGATTCACGCCCAGCCGGAGGTGT‑ATTGCAACACACTACAGGTGCCTCATCCTTCCGATCATATGTGGCAGGAGCGACTCGCCGATCGTCCCGGCATCAAGCAACTCGTCGCCTGTATTGATGGAGACGTCGTGGGCCATCTCACCATTGACGTGCAACAGCGCCCACGCCGCAGTCATGTTGCCGATTTTGGTATCTGTGTCGACTCTCGTTGGAAGAACCGCGGCGTCGCCAGCGCCCTGATGCGAGAGATGATTGAAATGTGCGACAA‑CTGGTTGCGGGTAGATCGCATTGAACTAACCGTGTTTGTCGATAACGCCCCGGCAATTAAGGTCTATAAAAAATACGGCTTTGAAATTGAAGGGACTGGTAAGAAGTACGCATTGCGTAATGGTGAATATGTCGATGCATATTATAT  >  NC_000913/3581023‑3581610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`