Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,224,895	C→T	L755L (CTG→TTG)	ebgA →	evolved beta‑D‑galactosidase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,224,895	0	C	T	100.0%	265.9 / NA	77	L755L (CTG→TTG)	ebgA	evolved beta‑D‑galactosidase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (41/36);  total (41/36)

GCCCCCTTGCAGATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGTGGCGCTTTCCGGCGAGCGTTACGGCGACTATCCGCACATCATTC  >  NC_000913/3224601‑3225183                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                  gCCCCCTTGCAGATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACt                                                                                                                                                                                                                                                                                              <  1:4667/300‑1 (MQ=255) gCCCCCGTGCAGATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAATGNAAACACCACGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCTGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACt                                                                                                                                                                                                                                                                                              <  2:229130/300‑1 (MQ=255)    cccTTGCAGATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCAGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTcgc                                                                                                                                                                                                                                                                                           >  1:448405/1‑300 (MQ=255)    cccTTGCAGATCACGCNGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACNTCCTGGCAGGTGAATGGCNAATCGCTGTTGACTcgc                                                                                                                                                                                                                                                                                           >  1:604951/1‑300 (MQ=255)      cTTGCAGATCACGCTGCCGCAGCTGGACGCCCGCGACGCGTTCCTCAACATTACGGTGACCAAAGATTCCCGAACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATCGTGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCAACATCCTGGCAGGTGAATGGCGAATCGCTGTTGAATCGCGa                                                                                                                                                                                                                                                                                         >  2:446521/1‑300 (MQ=255)                    tGCCGCAGCTGGATGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTTCCGCACCCGCTACATCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTGCGACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAAc                                                                                                                                                                                                                                                                            <  2:391133/299‑1 (MQ=255)                                   cccGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCgatga                                                                                                                                                                                                                                                            >  1:350782/1‑300 (MQ=255)                                        gAAGCGTTCCTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGAc                                                                                                                                                                                                                                                       <  1:8594/300‑1 (MQ=255)                                           gCGTTCCTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTTGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAAc                                                                                                                                                                                                                                                    <  1:265518/300‑1 (MQ=255)                                              ttCCTCAACATTACGGTGACCACAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGTAGGTGAATGGCGAATTGCTGTTGACTCGCGAGCCaa                                                                                                                                                                                                                                                                                    >  2:125035/1‑265 (MQ=255)                                                ccTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACaa                                                                                                                                                                                                                                               >  1:15512/1‑300 (MQ=255)                                                     aCATTACGGTGACCAANGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCNACTTCTTCAAGCCGATGANCGACAACCACAAGCa                                                                                                                                                                                                                                            >  1:607934/1‑298 (MQ=255)                                                     aCATTACGGTGACCAAAGATTCCCGCACCCTCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTAAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCa                                                                                                                                                                                                                                            >  1:483391/1‑298 (MQ=255)                                                     aCATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGACTGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCa                                                                                                                                                                                                                                            >  2:676376/1‑298 (MQ=255)                                                     aCATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCACGCCGATGATCGACAACCACAAGCa                                                                                                                                                                                                                                            >  1:270508/1‑298 (MQ=255)                                                     aCATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCAAAAGCa                                                                                                                                                                                                                                            >  1:271284/1‑298 (MQ=255)                                                     aCATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTAAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAgta                                                                                                                                                                                                                                            >  1:270522/1‑296 (MQ=255)                                                     aCATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCAACTATCAGTTCCCGCTGAAGGACAACACCGCGCAGCCAGTGCCTTTCGCACCCAATAATGCGCGTCCGCTGACGCTGGACGACGATCGCTTGAGCTGCACCGTTCGTGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGTAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGTCAAAGATCAACTTCTTCAAGCCGATGATCGACGACCACACGCa                                                                                                                                                                                                                                            >  2:676370/1‑298 (MQ=255)                                                          aCGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTAc                                                                                                                                                                                                                                     >  1:357099/1‑300 (MQ=255)                                                            ggTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTTAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGa                                                                                                                                                                                                                                   <  1:205350/300‑1 (MQ=255)                                                            ggTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGa                                                                                                                                                                                                                                   <  1:205433/300‑1 (MQ=255)                                                               gACCAAAGATTCCCGCACCCGCTACATCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACAGCGTAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACNATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACTAAgg                                                                                                                                                                                                                                <  2:218655/300‑1 (MQ=255)                                                               gACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTNCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAgg                                                                                                                                                                                                                                <  2:569859/300‑1 (MQ=255)                                                                    aaGATTCCCGCACCCGCTACAGCGAAGCCGGACACTCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGATTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGAGCGACAACCACAAGCAGGAGTACGAAgg                                                                                                                                                                                                                                <  2:241932/295‑1 (MQ=255)                                                                      gATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTgg                                                                                                                                                                                                                         <  1:636982/300‑1 (MQ=255)                                                                             gCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTCGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAAcc                                                                                                                                                                                                                    <  2:631270/298‑1 (MQ=255)                                                                                         gCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAATGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAg                                                                                                                                                                                                      <  2:25096/299‑1 (MQ=255)                                                                                                 ggACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGTGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAAACGAAGCCTTT‑GCAGATCa                                                                                                                                                                                                  >  2:175025/1‑295 (MQ=255)                                                                                                      cccTATCGCCACTTATNAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCNGTGGCAACCGAATCATTTNGCAGATCATGCAgggaa                                                                                                                                                                                         >  1:599054/1‑297 (MQ=255)                                                                                                      cccTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACa                                                                                                                                                                                        >  1:534311/1‑300 (MQ=255)                                                                                                      cccTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCACTT‑GCAGATCATGCAGGAACa                                                                                                                                                                                        >  2:579149/1‑300 (MQ=255)                                                                                                         tATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGTAACATc                                                                                                                                                                                      <  2:499487/299‑1 (MQ=255)                                                                                                          aTCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCNGCACCGTTCGCGGCTACAACTTCGCGATCACCNTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCt                                                                                                                                                                                     >  2:551022/1‑299 (MQ=255)                                                                                                                aCTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGAc                                                                                                                                                                              >  1:488480/1‑300 (MQ=255)                                                                                                                   tATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATNGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACttt                                                                                                                                                                           >  1:151593/1‑300 (MQ=255)                                                                                                                       aGTTCCCGCTGAAGGAAAACACCGCGCAACCAGTGCCGTTCGCACCAAATAATGCGCGTCCGCTGACGCTGTAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACTAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCg                                                                                                                                                                       <  2:45380/300‑1 (MQ=255)                                                                                                                                  aaGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGATTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGc                                                                                                                                                            <  2:467401/300‑1 (MQ=255)                                                                                                                                    ggAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGa                                                                                                                                                          >  1:221458/1‑300 (MQ=255)                                                                                                                                    ggAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTAAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGa                                                                                                                                                          >  1:221657/1‑300 (MQ=255)                                                                                                                                    ggAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTAAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGCATCGCTGTTGACTCGCGAGCAAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGa                                                                                                                                                          >  2:42711/1‑300 (MQ=255)                                                                                                                                    ggAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGAGATCACCTTCTAAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCAGTAGAACAGAACGa                                                                                                                                                          >  2:43943/1‑300 (MQ=255)                                                                                                                                     gAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCATTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGAt                                                                                                                                                         <  2:85329/300‑1 (MQ=255)                                                                                                                                       aaaCACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATgg                                                                                                                                                       <  1:84828/300‑1 (MQ=255)                                                                                                                                              gcgcAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACTAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAg                                                                                                                                                  <  2:103530/298‑1 (MQ=255)                                                                                                                                                gcAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGGAGTGc                                                                                                                                               <  1:198716/299‑1 (MQ=255)                                                                                                                                                gcAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGc                                                                                                                                               <  1:197269/299‑1 (MQ=255)                                                                                                                                                gcAGCCAGTGCCTTTCGCAACAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGGGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGc                                                                                                                                               <  1:198742/299‑1 (MQ=255)                                                                                                                                                       gTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGatcatc                                                                                                                                       >  1:237675/1‑300 (MQ=255)                                                                                                                                                            tttCGCACCAGATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTATAATTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACTAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGc                                                                                                                                    <  2:155573/298‑1 (MQ=255)                                                                                                                                                                     aaaTAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGCGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAAACGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGCAGCACAGAGCGATGGTGAAGTGCTGATCATCAGCAGCACAGtcat                                                                                                                         >  2:196722/1‑297 (MQ=255)                                                                                                                                                                     aaaTAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTAt                                                                                                                         >  1:45878/1‑300 (MQ=255)                                                                                                                                                                     aaaTAATGCGCGTCCGCTGACGCTGGAAGACGACCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCAGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGAACAGGTAt                                                                                                                         >  2:21216/1‑300 (MQ=255)                                                                                                                                                                         aatGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGATTGGCNAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGcc                                                                                                                     <  2:560618/300‑1 (MQ=255)                                                                                                                                                                         aatGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGcc                                                                                                                     <  1:185077/300‑1 (MQ=255)                                                                                                                                                                                   cGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGANTGGCGAATCGCTGTTGACTCGCGAGCCAAAGANCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGAGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGgtgt                                                                                                           >  2:538191/1‑300 (MQ=255)                                                                                                                                                                                   cGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGgtgt                                                                                                           >  2:608340/1‑300 (MQ=255)                                                                                                                                                                                                       cGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGc                                                                                       >  1:566199/1‑300 (MQ=255)                                                                                                                                                                                                         tttGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCAc                                                                                     <  2:350782/300‑1 (MQ=255)                                                                                                                                                                                                            gAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAGTGAGTGGCAAACCGACATCCTGGCAGGTGAATGGTGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGTAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCAGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGGTATTGCACCGCAGGTGTTTGACTTCGGGATGCGCTGCACCTa                                                                                  >  2:260293/1‑300 (MQ=255)                                                                                                                                                                                                            gAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTAAAGCCGATGATCGAAAACCACAAGCAGGAATACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCAGTAGAACAAAGCGATGGTGAAGTGCTGATCATCAGCAGCATAGTTATTGCCCCGCCGGTGTTTGACCTCGGGATGCGCTGCACCt                                                                                   >  2:331873/1‑299 (MQ=255)                                                                                                                                                                                                                tGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATc                                                                              <  1:334682/300‑1 (MQ=255)                                                                                                                                                                                                                 gtaCCGTTCGCGGCTACAACTTCACGACCACCTTCTCAAAAATGAGTGGCAAACCGACATCCCGGTAGGTGAATGGCGAATCGCTGTTGCATAGCGAGCCAACGATCAACTTCTTCAAGCCGCTGATCGCCAACCACAAGAAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑ATAGATCATGCCGGAACATCTGCGCGACTTTGCCGTAGAACCGAGCGATGGCGAAGTGCTGATCATCAGAAGCACAGTTATTGCACAGCCGGTGTTTGACTTCGGGATGCGTTGCACCTATATCt                                                                             >  2:255974/3‑300 (MQ=255)                                                                                                                                                                                                                      gTTCGCTGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGTCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCANAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGgc                                                                          <  2:221657/298‑1 (MQ=255)                                                                                                                                                                                                                      gTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCANAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGgc                                                                          <  2:221458/298‑1 (MQ=255)                                                                                                                                                                                                                            ggCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATc                                                                     >  2:689904/1‑297 (MQ=255)                                                                                                                                                                                                                                     ttCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGATCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGc                                                         <  2:486293/300‑1 (MQ=255)                                                                                                                                                                                                                                           agcACCTCCTCAAAAATGAGTGGCAAACAGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTTGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGtt                                                   <  2:65816/298‑1 (MQ=255)                                                                                                                                                                                                                                            tCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGtt                                                   <  2:604951/299‑1 (MQ=255)                                                                                                                                                                                                                                                  tctcAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACTAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGt                                              <  2:316311/298‑1 (MQ=255)                                                                                                                                                                                                                                                      aaaaaTGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGTGgcgc                                         <  1:130994/299‑1 (MQ=255)                                                                                                                                                                                                                                                       aaaaTGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCTAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGTGGCGCtt                                       >  1:183625/1‑300 (MQ=255)                                                                                                                                                                                                                                                       aaaaTGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCACGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGTGGCGCtt                                       >  2:483895/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  cAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGTGGCGCTTTCCGGCGAGc                             >  1:581111/1‑299 (MQ=255)                                                                                                                                                                                                                                                                             ccTGGCAGGTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGTGGCGCTTTCCGGCGAGCGTTACGGCGACt                 >  2:641005/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 gCAGTTGAATGTCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCATGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCATGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTTAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGTGGCGCTTTCCgg                                  <  2:268617/279‑1 (MQ=255)                                                                                                                                                                                                                                                                                     gTGAATGGCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATNATTT‑GCAGATCATGCAGGAACATCTGCGCGACNTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACCGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGTGGCGCTTTCCGGCGAGCGTTACGGTGACCATCCGcac         >  2:546716/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            gCGAATCGCTGTTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGTGGCGCTTTCCGGCGAGCGTTACGGCGACTATCCGCACATCATTc  <  1:489834/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                  GCCCCCTTGCAGATCACGCTGCCGCAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGGTGACCAAAGATTCCCGCACCCGCTACAGCGAAGCCGGACACCCTATCGCCACTTATCAGTTCCCGCTGAAGGAAAACACCGCGCAGCCAGTGCCTTTCGCACCAAATAATGCGCGTCCGCTGACGCTGGAAGACGATCGTTTGAGCTGCACCGTTCGCGGCTACAACTTCGCGATCACCTTCTCAAAAATGAGTGGCAAACCGACATCCTGGCAGGTGAATGGCGAATCGCTGCTGACTCGCGAGCCAAAGATCAACTTCTTCAAGCCGATGATCGACAACCACAAGCAGGAGTACGAAGGGCTGTGGCAACCGAATCATTT‑GCAGATCATGCAGGAACATCTGCGCGACTTTGCCGTAGAACAGAGCGATGGTGAAGTGCTGATCATCAGCCGCACAGTTATTGCCCCGCCGGTGTTTGACTTCGGGATGCGCTGCACCTACATCTGGCGCATCGCTGCCGATGGCCAGGTTAACGTGGCGCTTTCCGGCGAGCGTTACGGCGACTATCCGCACATCATTC  >  NC_000913/3224601‑3225183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑