Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,293,781	2 bp→AT	coding (239‑240/597 nt)	nrfG →	putative formate‑dependent nitrite reductase complex subunit NrfG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,293,781	0	G	A	100.0%	299.2 / NA	85	G80D (GGC→GAT)	nrfG	putative formate‑dependent nitrite reductase complex subunit NrfG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/48);  total (37/48)
*	NC_000913	4,293,782	0	C	T	100.0%	287.2 / NA	85	G80D (GGC→GAT)	nrfG	putative formate‑dependent nitrite reductase complex subunit NrfG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/48);  total (37/48)

AGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGGCGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCAAGCCATCGAACTATGGCAAAAAGTGATGGATCTCAACTC  >  NC_000913/4293489‑4294066                                                                                                                                                                                                                                                                                                           ||                                                                                                                                                                                                                                                                                             aGTGGTGTTGTTACTGCTGATTGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTAc                                                                                                                                                                                                                                                                                        <  1:401704/300‑1 (MQ=255) aGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTAc                                                                                                                                                                                                                                                                                        <  1:401858/300‑1 (MQ=255)     gtgtTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATc                                                                                                                                                                                                                                                                                    >  1:534197/1‑300 (MQ=255)      tgttgtTACTGCTGATTGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑NTCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGNCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCt                                                                                                                                                                                                                                                                                   <  2:550782/300‑1 (MQ=255)      tgttgtTACTGCTGATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCt                                                                                                                                                                                                                                                                                   <  1:594446/300‑1 (MQ=255)      tgttgtTACTGCTGATGGCACTGATCCGCTGGCGAGTGAGGGCGAAGCGATG‑AAACAGCCCAAAATACCTGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCGGTGTCGGCAGTTATCTGTTAAGCCCAAAATTGCATGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCt                                                                                                                                                                                                                                                                                   <  2:239544/300‑1 (MQ=255)      tgttgtTACTGCTGACGGCACTAATCCTCTTGCGAGTGAGGGCGAATCGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑TTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATTGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCt                                                                                                                                                                                                                                                                                   <  2:262651/300‑1 (MQ=255)      tgttgtTACTACTGATGGCACGGATCCTCGGGCGAGTGAGGTCTAAGCGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑CGGACAACACGCACGATATTGATGGTACTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATGGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCt                                                                                                                                                                                                                                                                                   <  2:74707/300‑1 (MQ=255)       gttgttACTGCTGATGGCACTGACCCTCTTGCGAGTGAGGGCGAAGCGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCt                                                                                                                                                                                                                                                                                   <  2:151615/299‑1 (MQ=255)           ttACTGCTGATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGc                                                                                                                                                                                                                                                                              <  1:94603/300‑1 (MQ=255)             tgctggttgcacttatcctcttgctagtgTGAGGGCGAAGCGATGGAAACAGCCCAAAATACCGGTGAAAATGGCTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGTTATCAGCGTCAGCGCGGATCCGCTACAGTCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAGGCGAACAGTGGGCGTTACTGGATGAGTACTATCt                                                                                                                                                                                                                                                                                   <  2:595530/271‑1 (MQ=255)                gctgATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAAc                                                                                                                                                                                                                                                                         >  1:246603/1‑300 (MQ=255)                  tgATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGa                                                                                                                                                                                                                                                                       >  1:242366/1‑300 (MQ=255)                         aCTGATCCTCTGGCGAGTGAGGGCGAAGCGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCATGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAg                                                                                                                                                                                                                                                                 <  2:638014/299‑1 (MQ=255)                         aCTGATCCTCTGGCGAGTGAGGGCGAAGCGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAg                                                                                                                                                                                                                                                                 <  1:697045/299‑1 (MQ=255)                          cTGATCCTCTGGCGAGTGAGGGCGAAGCGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCANTTATCTGTTAAGCCCAAAATGGCAGGCGGTACNTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTAATGGATGAGTACTATCTGTGGCAAAACGATTACAGCa                                                                                                                                                                                                                                                               >  2:543969/1‑300 (MQ=255)                                 tctGGCGAGTGAGGGCGAAGCGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTATCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGtt                                                                                                                                                                                                                                                        >  2:382863/1‑300 (MQ=255)                                   tGGCGAGTGAGGGCGAAGCGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGc                                                                                                                                                                                                                                                      <  1:245867/300‑1 (MQ=255)                                         gTGAGGGCGAAGCGATG‑AAACAGCCAAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGTGGTACGTGCGGAGT‑ATTAGCGTCAGCGCG‑GTCCGCTACA‑TCAGTTTGCAAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGTGTTATTGGATGAGTACTATCTGTGGCAAAATAATCACAGAGATTTGTTGCTGGCGt                                                                                                                                                                                                                                                >  2:255802/1‑300 (MQ=255)                                           gAGGGCGAAGCGATG‑AAACAGCCCAAAATACCGTTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTAc                                                                                                                                                                                                                                              <  1:179519/300‑1 (MQ=255)                                                   aGCGATG‑AAACAGCCCANAATACCGGTGAAAATG‑CTNACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGNTGGCGTACCGTCAGGc                                                                                                                                                                                                                                      <  1:608953/300‑1 (MQ=255)                                                   aGCGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCGAAACGATTAAAGCAATTCGTTGCTGGCGTACCGTCAGGc                                                                                                                                                                                                                                      >  1:118989/1‑300 (MQ=255)                                                    gCGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATNCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAAAAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGc                                                                                                                                                                                                                                      >  2:516172/1‑299 (MQ=255)                                                    gCGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGc                                                                                                                                                                                                                                      >  1:270590/1‑299 (MQ=255)                                                     cGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGt                                                                                                                                                                                                                                    >  1:16779/1‑300 (MQ=255)                                                      gagg‑AAACAGCCCAAAATATCTTTGAAAATG‑CTGACAACACTCACGATAGTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCg                                                                                                                                                                                                                                     <  2:52037/295‑1 (MQ=255)                                                            aaCAGCCCAAAATACCGTTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGTAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAAc                                                                                                                                                                                                                              <  2:636572/300‑1 (MQ=255)                                                            aaCAGCCCAAAATACCGTTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAAc                                                                                                                                                                                                                              <  1:227088/300‑1 (MQ=255)                                                            aaCAGCCCAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAAc                                                                                                                                                                                                                              <  1:586331/300‑1 (MQ=255)                                                               aGCCCAAAATACCGGTAAAAATG‑CTGACAACACTCACGATATTGATAGTATTTCTCTGTGTCGGCAGTTATCTGGTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTa                                                                                                                                                                                                                            <  2:176751/299‑1 (MQ=255)                                                                            ggTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGNAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAAc                                                                                                                                                                                                                <  1:513006/298‑1 (MQ=255)                                                                            ggTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAAc                                                                                                                                                                                                                <  1:721427/298‑1 (MQ=255)                                                                                            aacaCTCACGATATTGATGGTATTTCTCTGTTTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATgcggc                                                                                                                                                                                               <  2:702324/300‑1 (MQ=255)                                                                                                    cGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGa                                                                                                                                                                                       >  2:51086/1‑300 (MQ=255)                                                                                                     gATATTGATGTTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCNCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGgcgc                                                                                                                                                                                             <  2:229826/293‑1 (MQ=255)                                                                                                      atatTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGAc                                                                                                                                                                                      >  1:465286/1‑299 (MQ=255)                                                                                                      atatTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGAGCTGGCGAc                                                                                                                                                                                      >  1:630143/1‑299 (MQ=255)                                                                                                      atatTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTCGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGAc                                                                                                                                                                                      >  1:630473/1‑299 (MQ=255)                                                                                                             tGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCttt                                                                                                                                                                              >  1:703135/1‑300 (MQ=255)                                                                                                                   ttCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTAcc                                                                                                                                                                        <  1:271540/300‑1 (MQ=255)                                                                                                                                      tCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGAc                                                                                                                                                     <  1:42390/300‑1 (MQ=255)                                                                                                                                                  aaaaTGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACt                                                                                                                                           <  1:51086/298‑1 (MQ=255)                                                                                                                                                  aaaaTGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACt                                                                                                                                           <  2:176411/298‑1 (MQ=255)                                                                                                                                                       ggCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCaa                                                                                                                                    <  1:1241/300‑1 (MQ=255)                                                                                                                                                               gTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTCTTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGAc                                                                                                                            >  1:638326/1‑300 (MQ=255)                                                                                                                                                               gTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGAc                                                                                                                            <  2:411669/300‑1 (MQ=255)                                                                                                                                                                 aCGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACaa                                                                                                                          <  1:717178/300‑1 (MQ=255)                                                                                                                                                                   gTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACaaa                                                                                                                         >  1:474857/1‑299 (MQ=255)                                                                                                                                                                        gAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGccccc                                                                                                                   >  1:356623/1‑298 (MQ=255)                                                                                                                                                                        gAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTc                                                                                                                   >  1:498199/1‑300 (MQ=255)                                                                                                                                                                          gT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGNGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTcgc                                                                                                                 >  1:517761/1‑300 (MQ=255)                                                                                                                                                                          gT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTcgc                                                                                                                 >  1:443284/1‑300 (MQ=255)                                                                                                                                                                          gT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCACAGACTCGCGCAATGATCGACAAAGCCCTc                                                                                                                   >  1:213046/1‑298 (MQ=255)                                                                                                                                                                           gtattagcgttcgcgcG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTCCAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTAACAGGCCAGCAAGCATATGACCGCCCAGACTCGCGCAATGATCGCAAAAGCCCtagc                                                                                                                 >  2:460512/13‑297 (MQ=255)                                                                                                                                                                             aTCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCGGGCGTTGCAACTACGTAGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTAACAGGCCAGCCAGCa                                                                                                                                                           >  2:266954/1‑256 (MQ=255)                                                                                                                                                                             aTCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCAGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTAAGTGGTGAGAACGATGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGAACGCCCAGACTCGCGCAATGATCGACAAAGCACTCgcgc                                                                                                               >  2:138454/1‑300 (MQ=255)                                                                                                                                                                                 gcgtcagcgCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTCAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGa                                                                                                           >  1:292298/1‑300 (MQ=255)                                                                                                                                                                                   gtcagcgCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCNGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGAc                                                                                                          <  1:532721/299‑1 (MQ=255)                                                                                                                                                                                             aTCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCNACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGaaa                                                                                                <  1:151913/300‑1 (MQ=255)                                                                                                                                                                                                             ttGCCAGCCAGCAAACCCCGTAAGCGCAGCTTCATGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGAtgc                                                                                 <  1:403520/300‑1 (MQ=255)                                                                                                                                                                                                             ttGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGAtgc                                                                                 <  2:675647/300‑1 (MQ=255)                                                                                                                                                                                                             ttGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGAtgc                                                                                 <  1:403879/300‑1 (MQ=255)                                                                                                                                                                                                             ttGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACGGGATGAGTACTATCTGTGGTAAAACGATTACAGCAATTCGTTNCTGGCGTACCGTCAGGCGTTGTAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGAtgc                                                                                 <  2:230703/300‑1 (MQ=255)                                                                                                                                                                                                                          aaCCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAANGATTACAGCAATTCGTTGCTGGCGTACCGTCANGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCACTGATGCTGCTGGCTTcc                                                                      >  2:538002/1‑298 (MQ=255)                                                                                                                                                                                                                            ccccGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGAt                                                                   >  1:53801/1‑299 (MQ=255)                                                                                                                                                                                                                            cccaggAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGAt                                                                   >  1:53632/5‑299 (MQ=255)                                                                                                                                                                                                                                      gcAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGTGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTGCCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGc                                                         <  2:654200/299‑1 (MQ=255)                                                                                                                                                                                                                                      gcAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGGCCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGc                                                         <  2:654131/299‑1 (MQ=255)                                                                                                                                                                                                                                      gcAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGc                                                         <  1:397303/299‑1 (MQ=255)                                                                                                                                                                                                                                        aGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGc                                                         <  2:459467/297‑1 (MQ=255)                                                                                                                                                                                                                                         ggggaaggaaTTGGAGGGTAAAATCCGCGCTAATCGAAAAAAAAG‑TGGGTGGTGGGCGTTAATGGATGAGTACCCTCTGTGGCAAAACGATTACAGCAGTTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAATGCTGAACTGTATGCGGCGCTGGTGACGGTGCTTTATTACCAGGCCGGCCAGCATATGACCGCCCAGACTCGAGCAATGATCGCCAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGACCTGATACTGCTGGCTTCCGATGTGTTTATGCGGGc                                                     >  2:592854/10‑300 (MQ=255)                                                                                                                                                                                                                                         gCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGc                                                     >  1:187525/1‑300 (MQ=255)                                                                                                                                                                                                                                         gCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGACCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGc                                                     >  2:207154/1‑300 (MQ=255)                                                                                                                                                                                                                                           ttCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCACTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTACGATGCGTTTATGCAGGCGa                                                   >  2:334448/1‑300 (MQ=255)                                                                                                                                                                                                                                                        ggATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTACAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGAACGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCACTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCAAGc                                      >  2:157320/1‑300 (MQ=255)                                                                                                                                                                                                                                                         gATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCAAGcc                                     <  1:235258/300‑1 (MQ=255)                                                                                                                                                                                                                                                                tCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTNGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCAAGCCATCGAAc                              <  1:133527/300‑1 (MQ=255)                                                                                                                                                                                                                                                                tCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCAAGCCATCGAAc                              <  2:474857/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           ccACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCg                                                    <  1:439386/267‑1 (MQ=255)                                                                                                                                                                                                                                                                           ccACAAAACAG‑CGAACAGTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCg                                                    >  2:439386/1‑267 (MQ=255)                                                                                                                                                                                                                                                                                aaaCAG‑CGAACAGTGGNCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGANCTACGCGCAAGCCATCGANCTATGGCAAAAAGTGAt              >  1:595681/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             ttGGGCATTACTGGATGAGTACTATCTGCGGCAAAACTATTACAGCAATTCGTTGCTGGCGTACCGTCATGCGTGGCAACTACGTGGTGAGAACGCTGAATTGTATGCGGCGCGGTCGACGATGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCAAGCCATCGAACTATGGCAAAAAGTGATGGATCTCAACTc  <  2:434288/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                             gTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCNCGCAATGATCGACAAAGCCCTCGCGCTGGACANTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCAAGCCATCGAACTATGGCAAAAAGTGATGGATCTCAACTc  <  2:534197/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                             gTGGGCGTTACTGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCAAGCCATCGAACTATGGCAAAAAGTGATGGATCTCAACTc  >  2:440138/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             gTGGGCGTTACGGGATGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCAAGCCATCGAACTATGGCAAAAAGTGATGGATCTCAACTc  <  2:434289/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                           ||                                                                                                                                                                                                                                                                                             AGTGGTGTTGTTACTGCTGATGGCACTGATCCTCTGGCGAGTGAGGGCGAAGCGATG‑AAACAGCCCAAAATACCGGTGAAAATG‑CTGACAACACTCACGATATTGATGGTATTTCTCTGTGTCGGCAGTTATCTGTTAAGCCCAAAATGGCAGGCGGTACGTGCGGAGT‑ATCAGCGTCAGCGCG‑ATCCGCTACA‑TCAGTTTGCCAGCCAGCAAACCCCGGAAGCGCAGCTTCAGGCATTGCAGGATAAAATCCGCGCTAATCCACAAAACAG‑CGAACAGTGGGCGTTACTGGGCGAGTACTATCTGTGGCAAAACGATTACAGCAATTCGTTGCTGGCGTACCGTCAGGCGTTGCAACTACGTGGTGAGAACGCTGAACTGTATGCGGCGCTGGCGACGGTGCTTTATTACCAGGCCAGCCAGCATATGACCGCCCAGACTCGCGCAATGATCGACAAAGCCCTCGCGCTGGACAGTAATGAAATCACCGCCCTGATGCTGCTGGCTTCCGATGCGTTTATGCAGGCGAACTACGCGCAAGCCATCGAACTATGGCAAAAAGTGATGGATCTCAACTC  >  NC_000913/4293489‑4294066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑