Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	78,533	A→C	M305L (ATG→CTG)	setA →	sugar exporter SetA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	78,533	0	A	C	98.9%	328.9 / NA	92	M305L (ATG→CTG)	setA	sugar exporter SetA
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base C (51/39);  minor base T (0/1);  total (52/40)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAA  >  NC_000913/78242‑78819                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                   aTGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGAAGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCNGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTCAACCGGATTGATTTTATTTCATAGCCGTCTGGCGtt                                                                                                                                                                                                                                                                                             >  2:216275/1‑299 (MQ=255)        gTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGCCCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGAc                                                                                                                                                                                                                                                                                       >  1:366537/1‑298 (MQ=255)        gTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGc                                                                                                                                                                                                                                                                                     >  1:366722/1‑300 (MQ=255)                  ggATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACtttt                                                                                                                                                                                                                                                                           >  2:584672/1‑300 (MQ=255)                  ggATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACtttt                                                                                                                                                                                                                                                                           >  1:684739/1‑300 (MQ=255)                  ggATAGTAACGTACGGATGTTATTTGTCGCATCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGAATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTAAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTAATAGCCGTCTGGCGtt                                                                                                                                                                                                                                                                                             >  2:584662/1‑282 (MQ=255)                      aGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAAc                                                                                                                                                                                                                                                                       <  1:49430/300‑1 (MQ=255)                           cGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCCGGAGTCCTGTTTTACAACGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACg                                                                                                                                                                                                                                                                      >  2:5014/1‑296 (MQ=255)                                  aTGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATc                                                                                                                                                                                                                                                           <  2:684739/300‑1 (MQ=255)                                    ggtattgttCGCCGCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCgg                                                                                                                                                                                                                                                         <  2:458896/292‑1 (MQ=255)                                    gTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCgg                                                                                                                                                                                                                                                         <  2:459088/300‑1 (MQ=255)                                               ccTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGc                                                                                                                                                                                                                                                >  2:409320/1‑298 (MQ=255)                                                 tCGATGTTAATGTGGACCTACAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGt                                                                                                                                                                                                                                            <  2:78728/300‑1 (MQ=255)                                                 tCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCgg                                                                                                                                                                                                                                              <  1:27465/298‑1 (MQ=255)                                                    aCGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTAtt                                                                                                                                                                                                                                         >  2:446798/1‑300 (MQ=255)                                                    aCGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTATGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGCTGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTAtt                                                                                                                                                                                                                                         >  2:199875/1‑300 (MQ=255)                                                        tAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTTGG‑a                                                                                                                                                                                                                                    <  1:176950/300‑1 (MQ=255)                                                        tAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑a                                                                                                                                                                                                                                    <  1:297076/300‑1 (MQ=255)                                                                aCCTGTAACACCATGTACAGTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTAttg                                                                                                                                                                                                                           <  2:445945/300‑3 (MQ=255)                                                                aCCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATgg                                                                                                                                                                                                                           <  1:163962/300‑1 (MQ=255)                                                                  cTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGtt                                                                                                                                                                                                                         >  2:693156/1‑300 (MQ=255)                                                                  cTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATAGtt                                                                                                                                                                                                                         >  2:698806/1‑300 (MQ=255)                                                                  cTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTAATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGtt                                                                                                                                                                                                                         >  2:172298/1‑300 (MQ=255)                                                                       acacCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAgg                                                                                                                                                                                                                    <  1:336128/300‑1 (MQ=255)                                                                       acacCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCATCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCa                                                                                                                                                                                                                      >  1:267158/1‑298 (MQ=255)                                                                         acCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGAt                                                                                                                                                                                                                  >  1:227712/1‑300 (MQ=255)                                                                           cATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCANTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCAGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTCTCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGAttt                                                                                                                                                                                                                >  2:220863/1‑300 (MQ=255)                                                                              gTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑t                                                                                                                                                                                                            >  1:673359/1‑300 (MQ=255)                                                                                aCATTATTGATATTCCGTTGTGGATCAGTAGCGATTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCGGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGc                                                                                                                                                                                                          <  2:459621/300‑1 (MQ=255)                                                                                              ccGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑ATGGAAGAGc                                                                                                                                                                                               >  2:155538/1‑296 (MQ=255)                                                                                                 ttGTGGATCAGTAGCGNGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGAAGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGACGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCCACTTTTTCACGCTGTATTTATCGGCCTTGTTGCGGGTATTGGGGATGTNTATGGTTTCAGGATTTAANTGC‑CTGGAAGAGc                                                                                                                                                                                               >  1:597674/1‑296 (MQ=255)                                                                                                 ttGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTCA‑TGCGCTGAAAGAGCGGGGGc                                                                                                                                                                                         >  1:241114/1‑300 (MQ=255)                                                                                                 ttGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑ATGGAAGAGCGGGGGCa                                                                                                                                                                                        >  1:190037/1‑300 (MQ=255)                                                                                                        tCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTacca                                                                                                                                                                                 <  1:67384/300‑1 (MQ=255)                                                                                                          aGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTaccac                                                                                                                                                                                <  2:418303/299‑1 (MQ=255)                                                                                                           ttAGCGAGTTAGGATTGCCATACAAACTGGCGGGTTTCCTGATGTGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTACGGTAAGCTGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCATCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATc                                                                                                                                                                                                                                                           <  2:66534/226‑1 (MQ=255)                                                                                                           gTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCt                                                                                                                                                                              <  1:385919/300‑1 (MQ=255)                                                                                                                gAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTAtt                                                                                                                                                                          >  1:97083/1‑299 (MQ=255)                                                                                                                 aGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTaccacc                                                                                                                                                                               >  2:318164/1‑293 (MQ=255)                                                                                                                 aGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTAttt                                                                                                                                                                         >  2:717107/1‑299 (MQ=255)                                                                                                                   ttAGGATTGCCAGACAAACCGTCGGGTTTCCTGATTGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTTGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTa                                                                                                                                                                                    <  2:699861/286‑1 (MQ=255)                                                                                                                      ggATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAAc                                                                                                                                                                   >  1:193361/1‑300 (MQ=255)                                                                                                                              aGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGTTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTTGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTc                                                                                                                                                           <  2:158562/300‑1 (MQ=255)                                                                                                                                  aaaCTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTAc                                                                                                                                                        <  1:352050/299‑1 (MQ=255)                                                                                                                                  aaaCTGGCGGGTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTCGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTAc                                                                                                                                                        <  2:321277/299‑1 (MQ=255)                                                                                                                                            gTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTc                                                                                                                                             <  1:688999/300‑1 (MQ=255)                                                                                                                                            gTTTCCTGATGGGGACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTANGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTc                                                                                                                                             >  1:508617/1‑300 (MQ=255)                                                                                                                                                        ggACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATNTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTa                                                                                                                                 >  1:156571/1‑300 (MQ=255)                                                                                                                                                         gACGGCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTCCCGGGGTAATTCTGGCTGGCGTTAt                                                                                                                                >  2:66942/1‑300 (MQ=255)                                                                                                                                                            ggCAGCTGGTCTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTc                                                                                                                              <  1:694350/299‑1 (MQ=255)                                                                                                                                                            ggCAGCTGGTCTGGAAATACCAGCAATAATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTc                                                                                                                              <  2:165972/299‑1 (MQ=255)                                                                                                                                                                    gtctggaAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTAACGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCaa                                                                                                                     >  1:213419/3‑300 (MQ=255)                                                                                                                                                                     tctggaAATACCAGCAATGATTCTTGCTTGCTACTATGTCAAACGGTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTACTTATCGGCATTGTTGCGGGTATTTGG‑ATG‑CTATGGTGTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAAt                                                                                                                    <  2:495580/299‑1 (MQ=255)                                                                                                                                                                      ctggaAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAAtt                                                                                                                   >  1:235507/1‑300 (MQ=255)                                                                                                                                                                          aaaTACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGcac                                                                                                               <  2:478347/300‑1 (MQ=255)                                                                                                                                                                          aaaTACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGcac                                                                                                               <  1:409320/300‑1 (MQ=255)                                                                                                                                                                                 aGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGAGGCAGCTACCACCTTATTTACTAACAGTATTTATACCGGGGTAATTCTGGCTGACGTTATTCAGGGAGCAATTGCACAAAGtt                                                                                                         >  2:283616/1‑299 (MQ=255)                                                                                                                                                                                            tggctggcTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCAc                                                                                                 >  2:329379/1‑296 (MQ=255)                                                                                                                                                                                                  gctACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTTGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCt                                                                                       <  2:490234/300‑1 (MQ=255)                                                                                                                                                                                                  gctACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCt                                                                                       <  1:216275/300‑1 (MQ=255)                                                                                                                                                                                                    tactaTGTCAAACGTTATGGTAAGCGGCGAATGATGTTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGNTTTTATTTCATAGACGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTAc                                                                                     <  1:510966/300‑1 (MQ=255)                                                                                                                                                                                                    tactaTGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTAc                                                                                     <  1:175919/300‑1 (MQ=255)                                                                                                                                                                                                        aTGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCGTAGCCGTCTGACGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTAACGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCAATTTGCTGTCTACt                                                                                    >  2:315382/1‑297 (MQ=255)                                                                                                                                                                                                         tGTCAAACGTTATGTGAAGCGGCGAATGATAGTCATAGCAGTGGCTGCAGGAGTACTGTTTTACACCGTATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCTGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGt                                                                                <  2:349759/300‑1 (MQ=255)                                                                                                                                                                                                         tGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCTNCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGt                                                                                <  2:560374/300‑1 (MQ=255)                                                                                                                                                                                                         tGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGt                                                                                >  2:40124/1‑300 (MQ=255)                                                                                                                                                                                                              aaCGTTATAGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGCATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTg                                                                           <  2:493816/300‑1 (MQ=255)                                                                                                                                                                                                                      ggTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTTGAAGAGCGGGGGCAGCTACCACCTTATTTACTCACAGTATTTCTACCGGGGTAGTTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAGTTGCGGTTAtt                                                                   >  1:247419/1‑300 (MQ=255)                                                                                                                                                                                                                                 aaTGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACAGCGGATTGATTTTATTTCATAGTCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCAGGTATTGGG‑ATG‑CTATGGTTTCCGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGAGGGCACTTTGCTGTCTACTGGGTAGTTGCGGTTATTTATGTTATCga                                                        >  1:337967/1‑299 (MQ=255)                                                                                                                                                                                                                                 aaTGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGc                                                        >  1:337538/1‑300 (MQ=255)                                                                                                                                                                                                                                       ggTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCattatt                                                  >  1:464352/1‑300 (MQ=255)                                                                                                                                                                                                                                       ggTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGAattatt                                                  >  2:600725/1‑300 (MQ=255)                                                                                                                                                                                                                                       ggTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTCGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCattatt                                                  >  1:389332/1‑300 (MQ=255)                                                                                                                                                                                                                                       ggTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTAACGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCattatt                                                  >  1:464362/1‑300 (MQ=255)                                                                                                                                                                                                                                       ggTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACCTTCTAATGCGGTATTTATCGATATTGTTGCGAGTATTTAG‑ATG‑CTATGGTTTAAGGATTTAA‑TGC‑ATGGAAGAGCGGGGGCAGCTACCACATTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAACTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCattatt                                                  >  2:650684/1‑300 (MQ=255)                                                                                                                                                                                                                                         tCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTAtttt                                                >  1:169776/1‑300 (MQ=255)                                                                                                                                                                                                                                                aGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTTTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTcctgtctcttatacacatctcgcag                                         <  1:172298/300‑25 (MQ=255)                                                                                                                                                                                                                                                     cggcAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCaaa                                      >  1:251974/1‑298 (MQ=255)                                                                                                                                                                                                                                                        cAGGAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTa                                 >  1:345593/1‑300 (MQ=255)                                                                                                                                                                                                                                                          ggAGTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTaaa                               >  2:344270/1‑300 (MQ=255)                                                                                                                                                                                                                                                            aGTACTTTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTGGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGTGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCTTGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGTGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTAAAGa                             <  1:5014/300‑1 (MQ=255)                                                                                                                                                                                                                                                             gTACTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTAATGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTAAAGAc                            >  2:125136/1‑300 (MQ=255)                                                                                                                                                                                                                                                                cTGTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑ATGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTAAAGACGtt                         >  2:505680/1‑300 (MQ=255)                                                                                                                                                                                                                                                                cTGTTTTACACCAGATTGACTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATCGGA‑CTG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGTGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCCGGCTAGCGTTATTCCGGAAACAACTGCACAAAGTTGGGAGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTCTCGCATTATTTTTACCCGCAAAggctcaaaaagtt                         >  2:505668/1‑289 (MQ=255)                                                                                                                                                                                                                                                                  gTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTAAAGACGttt                        >  1:438507/1‑299 (MQ=255)                                                                                                                                                                                                                                                                  gTTTTACACCGGATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTAAAGACGttt                        >  2:158658/1‑299 (MQ=255)                                                                                                                                                                                                                                                                     ttACACCGGATTGATTTTACTTCATAGCCGTCTTGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCTGCATTGTTGCGGGTATTTGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCATGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTtgatg                    <  2:366537/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     ttACACCAGATTGATTTTATTCCAGAGCCGTCTGGCGTGGATGACGCTGCAACTTTTTAACGCTTTATTTATCTGCATTGTTGCGGGTATTTGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCTGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGTGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTtgatg                    <  2:366722/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            ggATTGATTTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCATGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGa             >  1:258914/1‑300 (MQ=255)                                                                                                                                                                                                                                                                             gattgattTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGAc            <  1:446798/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  attTTATTTCATAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTCGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAg       <  2:156571/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       atttcaTAGCCGTCTGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTATCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGaa  <  1:693156/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                   ATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTATTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTGTGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCAGCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAGCGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTCTTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATTGTTGCGGGTATTGGG‑ATG‑CTATGGTTTCAGGATTTAA‑TGC‑CTGGAAGAGCGGGGGCAGCTACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGAGCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTTGTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGATGACGTGGACGATAGCGGAA  >  NC_000913/78242‑78819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑