breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 68,590 G→A Q487* (CAG→TAG)  araB ← ribulokinase
RA 134,196 +T coding (2582/2598 nt) acnB → AcnB
RA 154,182 C→T L415L (CTG→CTA htrE ← putative fimbrial usher protein HtrE
RA 156,068 A→G V45A (GTA→GCA)  yadV ← putative fimbrial chaperone YadV
JC JC 168,362 IS1 (–) +9 bp coding (879‑887/2244 nt) fhuA → ferrichrome outer membrane transporter/phage receptor
RA 203,297 G→A V246V (GTG→GTA lpxA → acyl‑[acyl‑carrier‑protein]‑‑UDP‑N‑ acetylglucosamine O‑acyltransferase
RA 203,307 A→T T250S (ACC→TCC)  lpxA → acyl‑[acyl‑carrier‑protein]‑‑UDP‑N‑ acetylglucosamine O‑acyltransferase
RA 218,872 G→A intergenic (‑97/+15) proS ← / ← trmO proline‑‑tRNA ligase/tRNA m(6)t(6)A37 methyltransferase
JC JC 233,968 IS2 (+) +5 bp intergenic (‑13/+55) mltD ← / ← gloB membrane‑bound lytic murein transglycosylase D/hydroxyacylglutathione hydrolase GloB
MC JC 255,892 Δ18,063 bp IS5‑mediated gpt[ykfC] 26 genes
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 292,019 C→T L174L (CTA→TTA)  yagJ → protein YagJ
RA 309,381 C→T D216N (GAT→AAT)  ecpB ← putative fimbrial chaperone EcpB
RA 309,985 T→A G14G (GGA→GGT ecpB ← putative fimbrial chaperone EcpB
RA 321,260 A→T intergenic (+179/‑348) rclR → / → ykgE DNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE
RA 322,812 C→T H159Y (CAT→TAT)  ykgF → putative amino acid dehydrogenase with NAD(P)‑binding domain and ferridoxin‑like domain
RA 348,736 C→T P19S (CCA→TCA)  prpB → 2‑methylisocitrate lyase
JC JC 362,204 IS1 (–) +9 bp coding (968‑976/1254 nt) lacY ← lactose permease
RA 410,666 C→T P175S (CCT→TCT)  mak → fructokinase
RA 421,027 C→T R14R (CGC→CGT proY → putative transporter ProY
JC JC 431,643 IS1 (+) +9 bp coding (363‑371/885 nt) tsx ← nucleoside‑specific channel‑forming protein Tsx
RA 451,961 T→A intergenic (‑351/+109) cyoA ← / ← ampG cytochrome bo3 subunit 2/muropeptide:H(+) symporter
RA 465,005 G→A V181V (GTC→GTT queC ← 7‑cyano‑7‑deazaguanine synthase
RA 472,849 A→G G84G (GGA→GGG glnK → nitrogen regulatory protein PII‑2
JC JC 481,307 IS1 (+) +8 bp coding (3090‑3097/3150 nt) acrB ← multidrug efflux pump RND permease AcrB
RA 495,622 C→T T168I (ACC→ATC)  htpG → chaperone protein HtpG
RA 506,799 G→A S95L (TCG→TTG)  ybaK ← Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase YbaK
RA 534,742 G→A G276D (GGT→GAT)  gcl → glyoxylate carboligase
MC JC 575,786 Δ3,398 bp IS5‑mediated [nmpC]borD [nmpC], essD, rrrD, rzpD, rzoD, borD
RA 582,597 C→T intergenic (+500/‑278) tfaD → / → tfaX putative tail fiber assembly protein TfaD/protein TfaX
RA 606,259 C→T intergenic (‑60/+6) ybdJ ← / ← ybdK DUF1158 domain‑containing protein YbdJ/putative glutamate‑‑cysteine ligase 2
RA 652,054 (T)6→5 intergenic (‑198/‑181) citC ← / → dpiB citrate lyase synthetase/sensor histidine kinase DpiB
RA 694,941 G→A L5L (CTC→CTT miaB ← isopentenyl‑adenosine A37 tRNA methylthiolase
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 708,774 G→A A147A (GCG→GCA chiP → chitooligosaccharide outer membrane channel
RA 716,722 C→T intergenic (+816/+224) ybfP → / ← potE lipoprotein YbfP/putrescine transporter PotE
RA 721,604 G→A R44C (CGT→TGT)  kdpE ← DNA‑binding transcriptional activator KdpE
RA 736,238 Δ1 bp intergenic (+19/‑207) ybfC → / → ybfQ uncharacterized protein YbfC/inactive transposase YbfQ
RA 780,624 C→T noncoding (71/76 nt) lysT → tRNA‑Lys
RA 789,580 C→A E134* (GAA→TAA)  galK ← galactokinase
JC JC 820,914 IS5 (+) +4 bp coding (122‑125/714 nt) ybhM → Bax1‑I family protein YbhM
RA 822,527 C→T E405K (GAA→AAA)  clsB ← cardiolipin synthase B
RA 883,396 C→T intergenic (‑8/‑277) ybjG ← / → mdfA undecaprenyl pyrophosphate phosphatase/multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 900,653 T→C intergenic (‑78/+213) artJ ← / ← artM L‑arginine ABC transporter periplasmic binding protein/L‑arginine ABC transporter membrane subunit ArtM
RA 908,169 C→T R38R (AGG→AGA ybjT ← putative NAD(P)‑binding protein YbjT
MC JC 989,673 Δ8 bp coding (677‑684/1203 nt) pncB ← nicotinate phosphoribosyltransferase
RA 1,034,054 G→T T267T (ACG→ACT hyaB → hydrogenase 1 large subunit
RA 1,038,477 2 bp→AA coding (738‑739/1545 nt) appC → cytochrome bd‑II subunit 1
RA 1,062,222 G→A E142K (GAG→AAG)  torD → trimethylamine‑N‑oxide reductase‑specific chaperone
RA 1,078,792 A→T W31R (TGG→AGG)  putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
RA 1,088,365 Δ1 bp coding (1493/2019 nt) pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine N‑deacetylase and beta‑1,6 glycoside hydrolase
JC JC 1,105,699 IS1 (+) +9 bp coding (286‑294/312 nt) ymdA → uncharacterized protein YmdA
RA 1,123,823 2 bp→AG coding (231‑232/648 nt) grxB ← reduced glutaredoxin 2
RA 1,147,300 C→T S169S (AGC→AGT yceD → DUF177 domain‑containing protein YceD
RA 1,147,792 T→A S58T (TCG→ACG)  plsX → putative phosphate acyltransferase
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
JC JC 1,186,408 IS2 (–) +5 bp coding (565‑569/1227 nt) pepT → peptidase T
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,194,922 G→A T10T (ACC→ACT rluE ← 23S rRNA pseudouridine(2457) synthase
RA 1,201,548 A→C T18P (ACC→CCA)  ymfI → uncharacterized protein YmfI
RA 1,201,550 C→A T18P (ACC→CCA ymfI → uncharacterized protein YmfI
RA 1,253,002 C→T intergenic (+17/+83) dhaR → / ← ycgV DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV
RA 1,270,336 C→T A4T (GCG→ACG)  ldrC ← small toxic polypeptide LdrC
RA 1,275,021 C→T P413L (CCT→CTT)  ychO → putative invasin YchO
JC JC 1,293,196 IS5 (+) +4 bp intergenic (‑274/‑328) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
RA 1,301,164 G→A intergenic (+617/‑18) insH21 → / → oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,308,684 T→C intergenic (‑39/‑104) clsA ← / → yciY cardiolipin synthase A/uncharacterized protein YciY
RA 1,320,154 G→A Q411* (CAA→TAA)  trpD ← anthranilate synthase subunit TrpD
RA 1,331,059 2 bp→AT coding (12‑13/2598 nt) topA → DNA topoisomerase 1
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,382,603 G→T P219P (CCG→CCT ompG → outer membrane porin G
RA 1,395,367 G→A R186C (CGT→TGT)  ynaI ← small conductance mechanosensitive channel YnaI
RA 1,405,758 G→A intergenic (‑7/‑221) mcaS ← / → smrA small regulatory RNA McaS/DNA endonuclease SmrA
MC JC 1,411,925 Δ23,060 bp [ttcA]ynaM 34 genes
RA 1,488,688 A→G N153D (AAC→GAC)  aldA → aldehyde dehydrogenase A
RA 1,497,385 4 bp→CAA coding (530‑533/1656 nt) opgD → glucans biosynthesis protein D
RA 1,505,527 C→T G185D (GGT→GAT)  ydcO ← putative transport protein YdcO
RA 1,515,161 T→A F134I (TTC→ATC)  ydcV → putative ABC transporter membrane subunit YdcV
RA 1,517,587 2 bp→TT intergenic (+25/‑60) ortT → / → ydcY orphan toxin OrtT/DUF2526 domain‑containing protein YdcY
RA 1,522,583 C→T Q161Q (CAG→CAA yncD ← putative TonB‑dependent outer membrane receptor
RA 1,541,037 A→G I518I (ATT→ATC narZ ← nitrate reductase Z subunit alpha
RA 1,541,058 G→A T511T (ACC→ACT narZ ← nitrate reductase Z subunit alpha
RA 1,591,711 C→A E156* (GAG→TAG)  hipA ← serine/threonine‑protein kinase toxin HipA
RA 1,629,247 C→T I11I (ATC→ATT ydfZ → putative selenoprotein YdfZ
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,660,102 C→T N678N (AAC→AAT ynfE → putative selenate reductase YnfE
RA 1,667,460 G→A Q369* (CAG→TAG)  mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,682,877 A→T *240L (TAA→TTA)  rstA → DNA‑binding transcriptional regulator RstA
RA 1,712,661 A→T intergenic (+503/‑108) nth → / → dtpA endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA
RA 1,748,841 C→T R240K (AGA→AAA)  ydhT ← uncharacterized protein YdhT
RA 1,757,981 C→T V249I (GTT→ATT)  ldtE ← L,D‑transpeptidase LdtE
RA 1,836,397 G→A E109K (GAG→AAG)  ynjB → putative ABC transporter periplasmic binding protein YnjB
RA 1,843,473 C→T P368L (CCG→CTG)  gdhA → glutamate dehydrogenase
RA 1,848,300 2 bp→TT coding (376‑377/552 nt) ydjA ← putative oxidoreductase YdjA
JC JC 1,854,298 IS5 (–) +4 bp coding (554‑557/759 nt) ydjF ← putative DNA‑binding transcriptional regulator YdjF
RA 1,860,493 G→A F280F (TTC→TTT ydjL ← putative zinc‑binding dehydrogenase YdjL
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,907,251 G→T A62E (GCA→GAA)  cspC ← transcription antiterminator and regulator of mRNA stability CspC
RA 1,956,004 A→C I335R (ATA→AGA)  torZ ← trimethylamine N‑oxide reductase 2
RA 1,968,630 C→A L245F (TTG→TTT cheR ← chemotaxis protein methyltransferase
RA 1,977,484 A→G W121R (TGG→CGG)  flhC ← DNA‑binding transcriptional dual regulator FlhC
RA 2,002,357 G→A A417V (GCT→GTT)  fliC ← flagellar filament structural protein
RA 2,023,559 A→G T223A (ACT→GCT)  fliR → flagellar biosynthesis protein FliR
RA 2,027,332 T→A E229V (GAA→GTA)  dgcQ ← diguanylate cyclase DgcQ
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,041,278 G→A intergenic (+160/‑97) msrQ → / → zinT membrane‑bound flavocytochrome MsrQ/metal‑binding protein ZinT
RA 2,043,488 G→A noncoding (70/90 nt) serU ← tRNA‑Ser
RA 2,052,885 G→A intergenic (+871/‑758) yeeJ → / → shiA inverse autotransporter adhesin/shikimate:H(+) symporter
RA 2,095,413 G→A A97T (GCG→ACG)  hisA → 1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino]imidazole‑4‑ carboxamide isomerase
RA 2,099,200 G→A F138F (TTC→TTT ugd ← UDP‑glucose 6‑dehydrogenase
RA 2,101,965 G→A A304V (GCG→GTG)  insH7 ← IS5 family transposase and trans‑activator
RA 2,110,045 G→A Q32* (CAA→TAA)  rfbC ← dTDP‑4‑dehydrorhamnose 3,5‑epimerase
RA 2,142,598 G→A L117L (CTC→CTT udk ← uridine/cytidine kinase
RA 2,144,705 G→A L480L (CTG→CTA dgcE → putative diguanylate cyclase DgcE
RA 2,149,659 G→A Q443* (CAG→TAG)  yegI ← putative serine/threonine‑protein kinase YegI
RA 2,149,705 G→T I427I (ATC→ATA yegI ← putative serine/threonine‑protein kinase YegI
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,173,448 C→T intergenic (‑575/+834) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,233,609 C→T Q4* (CAG→TAG)  yeiS → DUF2542 domain‑containing protein YeiS
JC JC 2,239,316 IS5 (–) +4 bp intergenic (‑27/+31) mglA ← / ← mglB D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit/D‑galactose/methyl‑galactoside ABC transporter periplasmic binding protein
RA 2,242,247 C→T G241R (GGG→AGG)  yeiB ← DUF418 domain‑containing protein YeiB
RA 2,247,134 A→C Y467D (TAC→GAC)  lysP ← lysine:H(+) symporter
RA 2,279,019 G→A P248S (CCG→TCG)  bcr ← multidrug efflux pump Bcr
JC JC 2,319,839 IS2 (+) +5 bp coding (34‑38/2850 nt) rcsC ← histidine kinase RcsC
RA 2,329,935 C→T intergenic (‑138/+4401) yfaQ ← / ← yfaT tandem DUF2300 domain‑containing protein YfaQ/DUF1175 domain‑containing protein YfaT
RA 2,354,286 A→C M8L (ATG→CTG)  glpB → anaerobic glycerol‑3‑phosphate dehydrogenase subunit B
RA 2,389,456 G→T G114V (GGA→GTA)  yfbP → uncharacterized protein YfbP
RA 2,404,402 G→A S71L (TCG→TTG)  nuoB ← NADH:quinone oxidoreductase subunit B
RA 2,481,608 A→G L190S (TTA→TCA)  emrY ← tripartite efflux pump membrane subunit EmrY
RA 2,498,392 A→C intergenic (‑97/‑279) alaC ← / → pyrS glutamate‑‑pyruvate aminotransferase AlaC/sensor histidine kinase PyrS
RA 2,517,590 G→A L81L (CTC→CTT pdeA ← putative c‑di‑GMP phosphodiesterase PdeA
RA 2,526,902 A→C intergenic (‑46/‑44) yfeR ← / → yfeH putative DNA‑binding transcriptional regulator YfeR/putative solute:Na(+) symporter
RA 2,546,804 T→A D44E (GAT→GAA murP → N‑acetylmuramic acid‑specific PTS enzyme IICB component/anhydro‑N‑acetylmuramic acid transporter
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,577,937 T→C E147E (GAA→GAG maeB ← malate dehydrogenase (oxaloacetate‑decarboxylating) (NADP(+))
RA 2,584,265 C→T G421S (GGC→AGC)  aegA ← putative oxidoreductase AegA
RA 2,584,397 +T coding (1129/1980 nt) aegA ← putative oxidoreductase AegA
RA 2,584,400 C→A D376Y (GAT→TAT)  aegA ← putative oxidoreductase AegA
RA 2,587,112 C→T A461V (GCA→GTA)  narQ → sensor histidine kinase NarQ
MC JC 2,615,407 Δ54 bp coding (422‑475/1062 nt) yfgO ← putative transporter YfgO
JC JC 2,698,402 IS30 (+) +2 bp coding (148‑149/636 nt) pgpC ← phosphatidylglycerophosphatase C
RA 2,745,600 A→G Y107H (TAC→CAC)  rimM ← ribosome maturation factor RimM
RA 2,774,947 G→A G544S (GGT→AGT)  yfjW → uncharacterized protein YfjW
RA 2,781,539 T→G Q396H (CAA→CAC ypjA ← adhesin‑like autotransporter YpjA
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,880,635 T→C L138L (TTA→TTG casD ← type I‑E CRISPR system Cascade subunit CasD
RA 2,893,657 A→C E334A (GAG→GCG)  ygcN → putative oxidoreductase with FAD/NAD(P)‑binding domain
RA 2,926,772 C→A G155G (GGC→GGA ppnN → nucleotide 5'‑monophosphate nucleosidase
RA 2,992,062 C→A intergenic (+303/‑32) ygeG → / → ygeH TPR repeat‑containing putative chaperone YgeG/putative transcriptional regulator YgeH
RA 3,025,601 (T)5→4 coding (1251/1401 nt) xanQ → xanthine:H(+) symporter XanQ
RA 3,043,140 C→T Q3Q (CAG→CAA yqfA ← hemolysin‑III family protein YqfA
RA 3,070,858 T→G K129N (AAA→AAC fbaA ← fructose‑bisphosphate aldolase class II
MC JC 3,131,341 Δ128 bp IS5‑mediated yghQ ← putative transport protein YghQ
RA 3,147,115 C→T intergenic (‑378/+482) yghW ← / ← yqhI DUF2623 domain‑containing protein YghW/protein YqhI
RA 3,194,816 A→T noncoding (50/145 nt)
A17A (GCA→GCT
sibD ←
ibsD →
small RNA SibD
putative toxic peptide IbsD
RA 3,207,549 Δ1 bp coding (179/606 nt) ttdB → L(+)‑tartrate dehydratase subunit beta
RA 3,218,794 C→T V95M (GTG→ATG)  aer ← aerotaxis receptor
RA 3,227,355 G→T M374I (ATG→ATT ygjI → putative transporter YgjI
JC JC 3,261,756 IS2 (+) +5 bp coding (659‑663/2295 nt) tdcE ← 2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase 4
RA 3,275,902 A→T E207D (GAA→GAT garD → GarD
RA 3,296,481 G→A V25M (GTG→ATG)  dolP → division and outer membrane stress‑associated lipid‑binding lipoprotein
RA 3,329,743 G→T D261Y (GAT→TAT)  dacB → peptidoglycan DD‑endopeptidase DacB
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,433,900 C→T L71L (CTA→TTA)  def → peptide deformylase
RA 3,444,923 T→C T103A (ACC→GCC)  rpsE ← 30S ribosomal subunit protein S5
RA 3,474,425 T→G K43T (AAA→ACA)  rpsL ← 30S ribosomal subunit protein S12
RA 3,486,175 G→A C19Y (TGC→TAC)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,486,502 C→T T128I (ACT→ATT)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,507,249 C→T D30N (GAC→AAC)  yhfT ← uncharacterized protein YhfT
RA 3,511,466 C→T V293V (GTG→GTA yhfZ ← putative DNA‑binding transcriptional regulator YhfZ
RA 3,525,566 T→C intergenic (+143/+23) mrcA → / ← nudE peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA/ADP‑sugar diphosphatase NudE
RA 3,537,999 C→T F205F (TTC→TTT yhgF → putative RNA‑binding protein YhgF
RA 3,553,214 G→A G44R (GGA→AGA)  malT → DNA‑binding transcriptional activator MalT
RA 3,555,047 G→T V655F (GTC→TTC)  malT → DNA‑binding transcriptional activator MalT
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
MC JC 3,582,181 Δ1,247 bp IS1‑mediated [yhhZ][yrhA] [yhhZ], [yrhA]
RA 3,624,545 T→A D56E (GAT→GAA yhhI → putative transposase
RA 3,631,356 2 bp→AG coding (1234‑1235/1623 nt) yhiJ ← DUF4049 domain‑containing protein YhiJ
JC JC 3,635,895 IS1 (+) +9 bp intergenic (+2/+305) yhiM → / ← yhiN inner membrane protein YhiM/putative oxidoreductase YhiN
RA 3,647,428 C→T A377V (GCG→GTG)  gor → glutathione reductase (NADPH)
RA 3,677,528 2 bp→TT coding (822‑823/2061 nt) yhjG ← AsmA family protein YhjG
RA 3,679,508 C→T T30T (ACC→ACT kdgK → 2‑dehydro‑3‑deoxygluconokinase
RA 3,686,705 G→A F823F (TTC→TTT bcsC ← cellulose synthase outer membrane channel
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,714,356 A→T I680K (ATA→AAA)  bisC ← biotin sulfoxide reductase
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,770,539 C→T V281M (GTG→ATG)  yibH ← inner membrane protein YibH
RA 3,773,173 Δ1 bp coding (893/1914 nt) mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,773,176 C→T S299F (TCT→TTT)  mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,817,167 C→T T164T (ACC→ACT yicC → putative RNase adaptor protein YicC
RA 3,872,012 Δ1 bp coding (987/1149 nt) dgoD ← D‑galactonate dehydratase
RA 3,887,188 C→T L121F (CTT→TTT)  mnmE → 5‑carboxymethylaminomethyluridine‑tRNA synthase GTPase subunit
RA 3,938,072 C→T A260V (GCG→GTG)  rbsK → ribokinase
RA 3,949,240 C→T E244K (GAG→AAG)  yifB ← putative magnesium chelatase YifB
RA 3,953,602 C→T S42L (TCG→TTG)  ilvD → dihydroxy‑acid dehydratase
RA 3,961,662 G→T G329V (GGC→GTC)  rep → ATP‑dependent DNA helicase Rep
RA 3,965,174 G→A H153Y (CAC→TAC)  rhlB ← ATP‑dependent RNA helicase RhlB
RA 3,968,683 G→A M256I (ATG→ATA rfe → UDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase
RA 3,969,748 T→C S240P (TCG→CCG)  wzzE → enterobacterial common antigen polysaccharide co‑polymerase
RA 3,997,332 G→A Q50Q (CAG→CAA yigB → 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase
RA 4,034,225 C→T L361L (CTG→TTG)  trkH → K(+) transporter TrkH
RA 4,048,596 G→A G544D (GGT→GAT)  polA → DNA polymerase I
RA 4,053,510 C→T intergenic (+92/+137) hemN → / ← yshB coproporphyrinogen III dehydrogenase/uncharacterized protein YshB
RA 4,058,080 C→T intergenic (‑46/‑327) glnA ← / → bipA glutamine synthetase/50S ribosomal subunit assembly factor BipA
RA 4,060,209 C→T N601N (AAC→AAT bipA → 50S ribosomal subunit assembly factor BipA
RA 4,061,758 G→A P198P (CCG→CCA yihM → putative TIM barrel domain‑containing protein YihM
RA 4,090,435 Δ1 bp coding (491/1071 nt) frvX ← peptidase M42 family protein FrvX
RA 4,103,300 T→A L160Q (CTG→CAG)  yiiM → 6‑hydroxyaminopurine reductase
RA 4,143,377 C→T S283L (TCA→TTA)  frwC → putative PTS enzyme IIC component FrwC
RA 4,193,996 T→G D70A (GAT→GCT)  thiE ← thiamine phosphate synthase
RA 4,215,670 G→T D65Y (GAT→TAT)  aceB → malate synthase A
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,331,890 C→T L463L (CTC→CTT proP → osmolyte:H(+) symporter ProP
RA 4,366,023 C→A V251F (GTT→TTT)  dcuA ← C4‑dicarboxylate transporter DcuA
RA 4,422,299 G→T L38L (CTG→CTT ulaD → 3‑keto‑L‑gulonate‑6‑phosphate decarboxylase UlaD
RA 4,437,890 C→T Q62* (CAG→TAG)  ytfI → protein YtfI
RA 4,473,900 C→T G52R (GGA→AGA)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,475,493 T→C F19F (TTT→TTC yjgL → protein YjgL
RA 4,476,605 G→A S390N (AGC→AAC)  yjgL → protein YjgL
RA 4,489,016 C→T E350K (GAA→AAA)  yjgR ← DUF853 domain‑containing protein YjgR
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,537,010 G→A H195H (CAC→CAT nanS ← N‑acetyl‑9‑O‑acetylneuraminate esterase
RA 4,537,018 G→A P193S (CCT→TCT)  nanS ← N‑acetyl‑9‑O‑acetylneuraminate esterase
RA 4,538,910 Δ1 bp coding (592/717 nt) nanC ← N‑acetylneuraminate outer membrane channel
RA 4,566,834 C→T G138D (GGT→GAT)  yjiN ← DUF445 domain‑containing protein YjiN
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1978484 1979270–1978503 20–787 35 [0] [2] 32 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3423870–3424234 3424438–3424238 5–569 8 [6] [4] 7 [rrlD] [rrlD]
* * ÷ NC_000913 4542050 4555485 13436 44 [0] [0] 51 [fimE]–uxuR [fimE],fimA,fimI,fimC,fimD,fimF,fimG,fimH,gntP,uxuA,uxuB,uxuR

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 257908 =NA (NA)11 (0.150) 11/472 2.7 30.6% noncoding (768/768 nt) IS1 repeat region
?NC_000913 1858973 = 25 (0.340)coding (1257/1380 nt) ydjK putative transporter YdjK
* ? NC_000913 381260 =NA (NA)21 (0.280) 20/476 1.8 55.3% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 923019 = 17 (0.230)coding (107/321 nt) clpS specificity factor for ClpA‑ClpP chaperone‑protease complex
* ? NC_000913 = 382590NA (NA)26 (0.350) 25/476 1.4 59.1% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 923023 18 (0.240)coding (111/321 nt) clpS specificity factor for ClpA‑ClpP chaperone‑protease complex
* ? NC_000913 1207790 =35 (0.470)14 (0.200) 13/444 2.4 35.6% coding (290/630 nt) ycfK protein StfP
?NC_000913 1209619 = 18 (0.260)intergenic (‑500/‑66) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 120780530 (0.410)51 (0.740) 48/444 0.4 68.9% coding (305/630 nt) ycfK protein StfP
?NC_000913 = 1209602 18 (0.260)intergenic (‑483/‑83) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 12994980 (0.000)23 (0.320) 23/468 1.5 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 1638286 =11 (0.150)28 (0.380) 25/476 1.4 71.8% coding (159/189 nt) ynfQ protein YnfQ
?NC_000913 = 1651548 NA (NA)noncoding (706/706 nt) IS2 repeat region
* ? NC_000913 = 163829010 (0.140)17 (0.230) 17/474 2.0 63.0% coding (155/189 nt) ynfQ protein YnfQ
?NC_000913 = 2070271 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 1858745 =14 (0.190)15 (0.200) 14/474 2.4 51.7% coding (89/1044 nt) ydjJ putative zinc‑binding dehydrogenase YdjJ
?NC_000913 1978503 = NA (NA)noncoding (768/768 nt) IS1 repeat region
* ? NC_000913 = 185875215 (0.200)23 (0.310) 20/476 1.8 60.5% coding (82/1044 nt) ydjJ putative zinc‑binding dehydrogenase YdjJ
?NC_000913 = 1979270 NA (NA)noncoding (1/768 nt) IS1 repeat region
* ? NC_000913 = 19784830 (0.000)35 (0.470) 33/476 1.0 100% intergenic (‑286/+35) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
?NC_000913 = 2070271 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 1978503 =NA (NA)51 (0.690) 51/476 0.4 100% noncoding (768/768 nt) IS1 repeat region
?NC_000913 4555486 = 0 (0.000)intergenic (+137/+4) uxuR/yjiC DNA‑binding transcriptional repressor UxuR/DUF2686 domain‑containing protein YjiC
* ? NC_000913 1979271 =2 (0.030)30 (0.430)
+TTAAGTAATTGAGTG
27/446 1.2 94.1% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 2996360 = NA (NA)intergenic (+2504/+12) ygeI/insD4 protein YgeI/IS2 insertion element protein InsB
* ? NC_000913 3583428 =NA (NA)44 (0.600) 40/474 0.7 100% noncoding (1/768 nt) IS1 repeat region
?NC_000913 = 4542049 0 (0.000)coding (13/597 nt) fimE regulator for fimA