Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,477,2650AC29.1% 83.5 / 14.4 55L122* (TTA→TGA) dsdCDNA‑binding transcriptional dual regulator DsdC
Reads supporting (aligned to +/- strand):  ref base A (15/24);  new base C (16/0);  total (31/24)
Fisher's exact test for biased strand distribution p-value = 1.15e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCA  >  NC_000913/2476986‑2477561
                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                            
tCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑t                                                                                                                                                                                                                                                                                        <  2:498807/300‑1 (MQ=255)
      gTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGc                                                                                                                                                                                                                                                                                   <  1:232797/299‑1 (MQ=255)
                 gTTGCTCCATTCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGTGCTACATACTGGCAGGATTTCTTCAGCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGc                                                                                                                                                                                                                                                                         <  2:55795/298‑1 (MQ=255)
                     cTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGGCGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTCAAGTC‑CCCCCACGCGGGAacc‑aaccaaaatcgggggagtgagggacgggaa                                                                                                                                                                                                                                                                    >  2:359037/1‑275 (MQ=255)
                     cTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTNAGCGAAATAGACGGATACCGGCGTGGAAAATC‑CCCCCATGCGGGAACC‑CACCCGCA‑TTTGGCGGTAGAGGGCCGGGaa                                                                                                                                                                                                                                                                   >  1:403206/1‑300 (MQ=255)
                         aTGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATAc                                                                                                                                                                                                                                                                <  1:554512/299‑1 (MQ=255)
                         aTGCATGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCAAAATCGATTCCGGCACGTTGCAGATTGAAGTTGTCATTACCAGTGAGCACGGTGAGTGAAATAGGCGGATACCGGCGTGTAAAGTC‑CCCTACTGTGGGAacc‑aaccaacctctggcggcggggggaagggaatgcc                                                                                                                                                                                                                                                                >  2:303735/1‑271 (MQ=255)
                          tGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACag                                                                                                                                                                                                                                                              <  1:454174/300‑1 (MQ=255)
                                              aCGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAATC‑AACTAATGCGGGCAAC‑AACCAGCA‑TTGGGCGATAGAGGGACGGGAATCCAAAGTTGACGTTCCCGATA‑ACt                                                                                                                                                                                                                                         >  2:132574/1‑300 (MQ=255)
                                                gTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGGAGAGTC‑ACCTACTGCTGGCACC‑AACCAACA‑TTGGGTGGTAGAGGGCCGGGGACACCGAGTTGACGTTCCCGAtcaaccc                                                                                                                                                                                                                                        >  2:494624/1‑294 (MQ=255)
                                                   cAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAATC‑ACCTCACGCGGGTacc                                                                                                                                                                                                                                                                                                   >  2:463757/1‑240 (MQ=255)
                                                     gTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGANGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGAtt                                                                                                                                                                                                                                  <  2:407379/300‑1 (MQ=255)
                                                             ggTTAATTACCGTGTTNGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAATC‑ACCTAATGCGGGCACC‑CACCCGCA‑CTTGGTGATAGGGGGCCGGGGATCCNAAGTTCACATTCCCAATC‑NCTCCTTATTTTTGatat                                                                                                                                                                                                                          >  1:461199/1‑300 (MQ=255)
                                                                                   aGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGNCGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGt                                                                                                                                                                                                   <  2:164730/300‑1 (MQ=255)
                                                                                    gCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGGAAAATC‑AACTaa                                                                                                                                                                                                                                                                                                             >  2:98607/1‑197 (MQ=255)
                                                                                       tGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATcc                                                                                                                                                                                               <  1:227505/300‑1 (MQ=255)
                                                                                         tCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAg                                                                                                                                                                                             <  1:137448/300‑1 (MQ=255)
                                                                                         tCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAACGTGGGAACC‑AACCAACA‑CTGGGTGATAGAGGGACGGGGAGACAGAGGTAACGTTCCCGATC‑ACTCCCTATTTTTTATCTCCAGAACTTCCTGGTT‑CAGGGTATccca                                                                                                                                                                                             >  2:554836/1‑298 (MQ=255)
                                                                                         tCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCCCACGCGGGCACA‑CACCAACA‑CTTGGTGAGAGAGGGACGGGAACCCAAAGGTAACGTTCCCGATA‑ACCCCCGATCTTTGGCATCAAAAACTTCATGGTT‑CAGGGTaccc                                                                                                                                                                                               >  2:207471/1‑295 (MQ=255)
                                                                                                    aTTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGGAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCAAAATCGATTCCGGCACATTGCAGATTGAAGTTGTCATTAACAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGGAAAGTCCACC‑CCCGTGGGCACC‑AACAAGCA‑CTGGGAGGGAGGGGACCAGGAATACAGAGTTACCACTCCCGGTACACACC‑GATTTTTGATATCAAGCATTTcccgtttccgggtcccccaagccgatttta                                                                                                                                                                                   >  2:142899/1‑272 (MQ=255)
                                                                                                    aTTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGAAGGATACCGGCGTGTAAAGTC‑ACATCACGTGGGCacc‑aa                                                                                                                                                                                                                                                                                                >  2:142920/1‑193 (MQ=255)
                                                                                                           gCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTCAAATC‑ACCCCACACGGGAACC‑AACCCACACTTGGG‑GATAGAGGGCCGGGGATACCGAGTTAACATTCCCGATC‑ACTCCTTATTTTTTATATCCAAAACTTCCTGGTT‑CCGGGTCTCCCACGACGACTTTCGCGccc                                                                                                                                                                            >  2:530570/1‑299 (MQ=255)
                                                                                                                    tGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACg                                                                                                                                                                  <  1:314725/300‑1 (MQ=255)
                                                                                                                    gggcaggagggaggcagcaagcAGAAAGTGATGAGTGAGTTGCGCTGACGGCGAATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACg                                                                                                                                                                  <  1:314500/279‑1 (MQ=255)
                                                                                                                                                                            catcaAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAATC‑ACCCCACGCGGGAACC‑AACCAGCA‑TTGGGTGAGAGAGGGGCGGGAATAAAGAATTAACATTCCNCACC‑ACCCCCGATTTTTTGTACCCAGAATTTCCTGGTTTCAGG‑TATCCAACGAAGAACTTAGCGCCCAACGAACACGGTTCCCCCCGTGCGTTGATTCCACTTTTGGATGGGAACAAACAAACCAATGAAt                                                                                                            >  2:179305/1‑298 (MQ=255)
                                                                                                                                                                            catcaAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGGAAAATC‑ACCTCACGCGGGCacc‑aacc                                                                                                                                                                                                                                                                                              >  1:271074/1‑123 (MQ=255)
                                                                                                                                                                                   gTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCCAACGCGGGCACC‑AACCAACA‑TTGGGCGATAGAGGGACGGGAATCCAAAGTTCACGTTCCCAATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CCGGGTATCCCGCGACGATTTTCGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTTCGATGGGAACGAACCAAAAAATGAATGTCCAAtt                                                                                                    >  1:296118/1‑299 (MQ=255)
                                                                                                                                                                                          gCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGTTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAAttcttctt                                                                                              <  2:133775/298‑1 (MQ=255)
                                                                                                                                                                                             aaaTCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAACTCTTCTTCCAGc                                                                                         <  1:384613/300‑1 (MQ=255)
                                                                                                                                                                                                         gCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACgg                                                                             <  1:545288/300‑1 (MQ=255)
                                                                                                                                                                                                                  aGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTAc                                                                     <  1:552532/299‑1 (MQ=255)
                                                                                                                                                                                                                   gATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCg                                                                   <  1:303735/300‑1 (MQ=255)
                                                                                                                                                                                                                   gATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAATC‑CCCCCACGCGGGCACC‑CACCAACC‑TTGGGCGATAGAGGGACGGGGATACAGAGTTCACGTTCCCAATA‑ACTCCTTATTTTTGATATCCAAAAGTTCCTGGTT‑CAAGGTATCCCGCAACGACTTTCGCGCCCAACAAACACATTTCCCCCCGTGTGTTAATTCCCCTTTGCGATGGGGAAGAAAAAAAAACTGAATGCCCCATTCTTCTTCCAGCTGATTGATACGGTggattacc                                                                    >  1:40960/1‑292 (MQ=255)
                                                                                                                                                                                                                      tGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCAc                                                                <  1:132202/300‑1 (MQ=255)
                                                                                                                                                                                                                        aCGTTGTCATTAACAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTCAAGTC‑CCCTCACGCGGGCACC‑CACCAgaa                                                                                                                                                                                                                                                                                          >  2:297879/1‑81 (MQ=255)
                                                                                                                                                                                                                          gTTGTCAGTATCAGTGAGCACGGGGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGNTTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTgg                                                             <  2:165109/299‑1 (MQ=255)
                                                                                                                                                                                                                             gTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTCACGTGGGTACC‑AACCAGCA‑TTTGGGGGTGGAGGGCCGGGAATACAGAGTTCACATTCCCAATC‑ACTCCTGATTTTTGATGTCCAAAATTTCCTGGTT‑CCGGGTATTCCGCGAAGATTTTAGCGCCCAACAAACACGTTTCCCCCCGTGTGTTCATTCCACCTTGCGAGGGGGACGAAAAAACAACCGAATTCACAATTCTTCTTCCCGCTGATTGATCCAGTGACTTACCGCAccagggat                                                         >  2:191454/1‑293 (MQ=255)
                                                                                                                                                                                                                             gTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCCAACGCAGGAACC‑AACCAACC‑CTGGGCGATAGAGGGACGGGAATACCGAGTTCACATTCCCAATC‑ACTCCCGATTTTTTATATCAAAAACTTCCCGGTT‑CAGGGTATCCAACGACGATTTTCGCGCCCAACAAACACGTTTCCCCCCATGCGTTAATTCCACCTTGAGATGGGGAAGAACAAACAACCTAATGTCCCATTCTTCTTTCCGCTTATTTATTCGGTGAATTCCCTCACTggggat                                                         >  1:443995/1‑298 (MQ=255)
                                                                                                                                                                                                                             gTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAACC‑CCCCCACGTGGGCACC‑AACCCACA‑CTGGGCGATAGAGGGCAAGGGATACAAAGTTCACATTCCCAATC‑ACTCCTGATTTTTGATATCCAGAATTTCCTTGTT‑CAGGGTATCCAAAAACGAGTTTCGCGCCCAATAAACACATTTCCCCCCGTGCGTTAATTCCAACTTGCGGTGGGAAAGAAAAAAAAAACGAAGGTCCAATTCTTCTTTCAGCTGATTGATACGGTGAATTACCGCACTggggat                                                         >  1:444310/1‑298 (MQ=255)
                                                                                                                                                                                                                                      aGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGAc                                                  <  1:494624/298‑1 (MQ=255)
                                                                                                                                                                                                                                      aGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAATC‑AACTCACGCGGGCACC‑AACCAGCA‑tt                                                                                                                                                                                                                                                                                       >  2:40180/1‑71 (MQ=255)
                                                                                                                                                                                                                                          aGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTcc                                            <  2:313576/300‑1 (MQ=255)
                                                                                                                                                                                                                                                  gAGCGAAATAGACGGGTACCGGCGTGTAAAGTC‑ACCCCATGCGGGCACC‑CACCCACA‑TTGGGGGATAGAGGGACGGGAACACAAAGTTCACGTTCCCAATA‑ACTCCTGATTTTTGATATCCAGAATTTCCCGGTT‑CAGGGTATCCCGCGACGATTTTAGCGCCCAACAAACACATTTCCCCCCGTGTGTTAATTCCCCTTTGCGATGGGGAAGAACAAACAACCGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTCCCGCCCTGGGGATCAGCGACAACTCCTCTgccgc                                    >  1:283566/1‑300 (MQ=255)
                                                                                                                                                                                                                                                  gAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCCCACGCGGGCACCCACCCACCT‑TTGGG‑GATAGAGGGCCGGGAACCCAGAGTTCACATTCCCAATA‑ACTCCTGATTTTTTATATCCAGAATTTCCTGGTT‑CAGGGTATCCAGCGAAAATTTTCGCACCCAACAAACACATTTCCCCTCGTGTGTTAATTCCACTTTTCGATGGGGACAAACAAACAACCGAATGCCCCATTCTTCTTCCCACTGATTGGTACAGTGACTTCCCGAAATGGGGCTCCGCGACAACTCCTCTtccga                                    >  1:283557/1‑299 (MQ=255)
                                                                                                                                                                                                                                                       aaaTAGACGGATACCGGCGTGTAAAATC‑ACCCAATGCGGGCACC‑AACCAACA‑TTGGGCGATAGAGGGCCAGGAATACAGAGTTCACGTTCCCGATA‑ACTCCTGATTTTTGATATCCAGAATTTCCTGGTT‑CAGGGTATCCCGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCCCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACCGAATGCCCAATTCTTCTTCCAACTGATTGATACGGTGACTTCCCGCACTGGGGATCCGCGACAATTCCTCTgccgaca                                  >  1:350201/1‑294 (MQ=255)
                                                                                                                                                                                                                                                               ggATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGAc                       <  1:139775/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                      ggCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATGTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCNTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCCCTGCCGCCAGGGCGAAGGACTGATGc                 <  2:403206/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                       gCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGc                 <  1:132574/298‑1 (MQ=255)
                                                                                                                                                                                                                                                                         gtgtAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCTATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCt               >  1:165599/1‑298 (MQ=255)
                                                                                                                                                                                                                                                                         gtgtAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGCACAAACAACTGAATGCCCAATTCTTATTCCAGCTGATTGATATGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGcat               >  2:62298/1‑296 (MQ=255)
                                                                                                                                                                                                                                                                         gtgtAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTATGCCGACAGGGCGAAGGACTGATGc                 >  2:62292/1‑296 (MQ=255)
                                                                                                                                                                                                                                                                         gtgtAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCAGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCTATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCt               >  1:166187/1‑298 (MQ=255)
                                                                                                                                                                                                                                                                             aaaGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGc         <  1:454817/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     ccTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGTGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACttaa  >  2:208559/1‑298 (MQ=255)
                                                                                                                                                                                                                                                                                     ccTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTATTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTATGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCa  >  2:322042/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                     ccTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCATCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCATGGCAGCCAATTCa  >  2:295981/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                            
TCATCCGTACCGGAGTCGTTGCTCCATGCCTGTCTGTCATGGAGCAACGTACAGTGACACAGGTTAATTACCGTGTTGGTTAAAGCATGTCTTTGAGCGTATTCCGGGCTACATACTGGCAGGATTTCTTCATCCATCAGAAAGTGATGAGTGAGTTGCGCTGACGGCGCATCATCAAAGTAGATCGCCAAATCGATTCCGGCACGTTGCAGATTGACGTTGTCATTACCAGTGAGCACGGTGAGCGAAATAGACGGATACCGGCGTGTAAAGTC‑ACCTAATGCGGGCACC‑AACCAGCA‑TTGGGCGATAGAGGGCCGGGAATACAGAGTTAACGTTCCCGATA‑ACTCCTGATTTTTGATATCAAGAATTTCCTGGTT‑CAGGGTATCCAGCGACGATTTTAGCGCCCAATAAACACGTTTCCCCTCGTGCGTTAATTCCACTTTGCGATGGGAACGAACAAACAACTGAATGCCCAATTCTTCTTCCAGCTGATTGATACGGTGACTTACCGCACTGGGGCTCAGCGACAATTCCTCTGCCGCCAGGGCGAAGGACTGATGCCTGGCAGCCACTTCA  >  NC_000913/2476986‑2477561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑