Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,175,991	T→A	K111* (AAG→TAG)	gatZ ←	putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,175,991	0	T	A	100.0%	200.6 / NA	62	K111* (AAG→TAG)	gatZ	putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (38/24);  total (38/24)

AGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATAT  >  NC_000913/2175696‑2176284                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                      aGCCTACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTTGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATAcc                                                                                                                                                                                                                                                                                                   <  2:264884/300‑1 (MQ=255) aGCCCACTTGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATAc                                                                                                                                                                                                                                                                                                    <  2:545522/299‑1 (MQ=255) aGCCCACGGGCAATAAATGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATAcc                                                                                                                                                                                                                                                                                                   <  2:586746/300‑1 (MQ=255) aGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATAcc                                                                                                                                                                                                                                                                                                   <  1:486133/300‑1 (MQ=255) aGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATAcc                                                                                                                                                                                                                                                                                                   <  2:582543/300‑1 (MQ=255) aGCCCACGGGCAATAAAGGCCTTTTGAGGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAAGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATAc                                                                                                                                                                                                                                                                                                    <  2:226942/299‑1 (MQ=255)                                        gTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCCCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCt                                                                                                                                                                                                                                                            >  2:563386/1‑300 (MQ=255)                                                              gggTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCa                                                                                                                                                                                                                                      >  1:316350/1‑300 (MQ=255)                                                              gggTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCACCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCAGGTACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGGTTggacaaa                                                                                                                                                                                                                                      >  1:316638/1‑295 (MQ=255)                                                              gggTGATGTGTACTGACTGAATGGCGCTGGCCTCAACGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGccc                                                                                                                                                                                                                                        >  1:317257/1‑298 (MQ=255)                                                                  gATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAgat                                                                                                                                                                                                                                   >  1:113288/1‑299 (MQ=255)                                                                  gATGTGTACTGACTGAATGGCGCTGGCCCCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAgat                                                                                                                                                                                                                                   >  2:585518/1‑299 (MQ=255)                                                                       gtACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATcgc                                                                                                                                                                                                                             >  1:171895/1‑300 (MQ=255)                                                                       gtACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATcga                                                                                                                                                                                                                             >  2:399414/1‑299 (MQ=255)                                                                                 aaTGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTGGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGaat                                                                                                                                                                                                                   <  1:418910/300‑1 (MQ=255)                                                                                  aTGGCGCTGTCCTCACCGCCCGGAACCGGGACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCTG‑‑CGCAGGACATTTACGCATCAAGATGAATTTTACTGAAGCCTGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGACCGCCGCCGAGaat                                                                                                                                                                                                                   <  2:99757/299‑1 (MQ=255)                                                                                     gcgcTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGaataat                                                                                                                                                                                                                >  1:32081/1‑299 (MQ=255)                                                                                     gcgcTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCAG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGaatagt                                                                                                                                                                                                                >  1:75945/1‑297 (MQ=255)                                                                                     gcgcTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCAGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGCAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGaataat                                                                                                                                                                                                                >  1:31999/1‑299 (MQ=255)                                                                                                   gCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCCGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCaaa                                                                                                                                                                                                 >  2:172291/1‑300 (MQ=255)                                                                                                       ggaaccggaacTTCGGTGCCAATTACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTACCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAAc                                                                                                                                                                                                <  2:28532/297‑1 (MQ=255)                                                                                                                          cAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGACCCAGATGATCGCCGCCGCGAATAATGCGTTTGCGTGCAAACCCAACCTTATCGACAATCGtca                                                                                                                                                                          >  2:77637/1‑298 (MQ=255)                                                                                                                               aCATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACa                                                                                                                                                                     >  2:390796/1‑300 (MQ=255)                                                                                                                                    gCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTc                                                                                                                                                                >  1:298139/1‑300 (MQ=255)                                                                                                                                           tGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACCTTATCGGCAATCGTAAAAACAAATTCGCGaaa                                                                                                                                                          >  1:52440/1‑299 (MQ=255)                                                                                                                                                  gCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCg                                                                                                                                                  <  2:555474/300‑1 (MQ=255)                                                                                                                                                                              agcaaagcaaATCACATCAACCCATTCCGCAACCATTTCTGGTGCTAACGGTATTGGATCCCCCG‑‑AGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCTCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTTTCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAAc                                                                                                                                <  2:549204/290‑1 (MQ=255)                                                                                                                                                                                      aaagcaCAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCt                                                                                                              <  1:438226/300‑1 (MQ=255)                                                                                                                                                                                         gcaCAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATACCCGCCACATTGATTGACCTGGt                                                                                                           >  2:622238/1‑300 (MQ=255)                                                                                                                                                                                         gaacaGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCANCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCAGGTATAACCGCCAAATTGATTGACCTGGt                                                                                                           >  2:458053/3‑300 (MQ=255)                                                                                                                                                                                                          gCAACCGTTTCTGGTGCTAACGGTAGGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATc                                                                                          <  2:19636/300‑1 (MQ=255)                                                                                                                                                                                                                gTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCGGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCAc                                                                                     <  2:9832/299‑1 (MQ=255)                                                                                                                                                                                                                     tGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTgc                                                                                <  2:592241/299‑1 (MQ=255)                                                                                                                                                                                                                              cGGTATGGGATCCCCCGGGCGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAAccttc                                                                                                                                                                                       >  1:214181/1‑185 (MQ=255)                                                                                                                                                                                                                              cGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGtt                                                                      >  1:399700/1‑300 (MQ=255)                                                                                                                                                                                                                                gTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGc                                                                    >  1:565801/1‑300 (MQ=255)                                                                                                                                                                                                                                gTATGGGATCCCCCG‑‑CGCAGGACATTGACACATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGc                                                                    >  1:565829/1‑300 (MQ=255)                                                                                                                                                                                                                                  aTGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAACTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTTTTGCGa                                                                  >  2:212288/1‑300 (MQ=255)                                                                                                                                                                                                                                     ggATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCa                                                               >  1:93629/1‑300 (MQ=255)                                                                                                                                                                                                                                              g‑‑cgcAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAgc                                                      >  1:160498/1‑300 (MQ=255)                                                                                                                                                                                                                                              g‑‑cgcAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGAAGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAgc                                                      >  1:501836/1‑300 (MQ=255)                                                                                                                                                                                                                                                     ggACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGCTCAAATGCCAGcgccgc                                                 >  2:531892/1‑300 (MQ=255)                                                                                                                                                                                                                                                     ggACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCAGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTTCGATTAACTGCCAGcgccgc                                                 >  2:67448/1‑300 (MQ=255)                                                                                                                                                                                                                                                               gCATCAAGATTAATTTTACTTAAGACGGCACAAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTTACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAAcc                                       <  2:75945/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           aTTTTACTGAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCAGGTGTCATTCCGGTATACCCGCAAAATTGCTTGACCTGGTTTGACGTTTCTTCAATCCGCACTTTGCGCGTGTTGTTGCGATCAAATGCCAGTGCCGATTCGATAACCAACGGATGGGCa                           >  2:167665/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   gAAGCCGGCACGAACATATTCCTATACCAGCTCTACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATAGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGAc                   <  1:327567/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   gAAGCCGGCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATAGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGAc                   <  1:328291/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   gAAGCCGGCACGAACATATTCCTATACCAGCTAGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGAc                   <  1:327746/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   gAAGCCGGCAAGAACATATTCCTATACCAGCTAGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAACAATGCGTTCGCGTGCAAACCCAACTTTATAGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGAc                   <  1:326509/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         ggCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACa             >  1:443754/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         ggCACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCAGGTATAAACGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGCACAGACTGAACa             >  2:90478/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         ggCACGAACATATTCCTATACCAACTCGACGGATCTTTCCATCACCGCATCCGCACTTTCTTGCTGCCAGCAGTTTGGCCCCCGATGATCGCCGCCGAGGATACTGCGTTCGCGTGCAAACCCAACTTTATCGGCAACCGTAAAAACAAAATCACAAAAGTCCGCCGGTGTCCTTACGGTTTAACAGCCGACTTGATTGACCGGGTTTGACTTTGTTTAAATTAGCACTTTGTGCGTgtttttgtgattacatgccc                                                        >  2:90205/1‑238 (MQ=255)                                                                                                                                                                                                                                                                                              gAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGc        <  2:449777/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              gAACATATTCCTATACAAGATCGACGGATTGTTCCATCGCCGCATCCGCATGTGCGTGCTGCCAGCAGTTTGGCACCAGATGATAGACGCCGAGAATAATGCGGTCGCGTGCAAACCCAACTTTATCGGCAAGCGTAAGAAAAAATTAGCGAAAGTCTGCCGGTGTCATTCCGGGATAATCGCCAAATTGATTGACCTGGTTTGACGGTGCTTCAATCAGCACTTTGAGCGTGTTGTGGAGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATg         <  1:278022/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                               aaCATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCACCTTTATCGGCAATCGTAACAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGAAAAAttgattgaa                                                                                                                >  2:401982/1‑194 (MQ=255)                                                                                                                                                                                                                                                                                               aaCATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGcc       >  1:35922/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               aaCATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGAAAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCCCttt                                                                                  >  2:120414/1‑225 (MQ=255)                                                                                                                                                                                                                                                                                                 cATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCAGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTCTCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCAGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCaa                                     >  2:76776/1‑268 (MQ=255)                                                                                                                                                                                                                                                                                                   tatTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGata   <  2:32081/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   tatTCCTACACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCACCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCTGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGata   <  2:31999/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     ttCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGatat  >  1:197130/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                     ttCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACCTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCGTTCAGGTATAACCGACAAATTGATTGACATGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATTAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCCGAACAGACTGAACATATGCAGatat  >  2:210802/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                      AGCCCACGGGCAATAAAGGCCTTTTGATGCGTACGTAAAGTATTGGCGGCATCTTCAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCG‑‑CGCAGGACATTGACGCATCAAGATGAATTTTACTGAAGCCGGCACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATCAAATGCCAGCGCCGCTTCGATAACCAACGGATGGGCAGAACAGACTGAACATATGCCGATAT  >  NC_000913/2175696‑2176284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑