Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,738,377	C→T	H178H (CAC→CAT)	purR →	DNA‑binding transcriptional repressor PurR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,738,377	0	C	T	100.0%	224.1 / NA	65	H178H (CAC→CAT)	purR	DNA‑binding transcriptional repressor PurR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/39);  total (26/39)

GCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACG  >  NC_000913/1738097‑1738672                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                        gCTTCCAGAAAGGTTACACCCTTGTTCTGGGCAATGTGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATcc                                                                                                                                                                                                                                                                                      <  2:44480/300‑1 (MQ=255) gCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATcc                                                                                                                                                                                                                                                                                      <  1:484527/300‑1 (MQ=255)            ggTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCt                                                                                                                                                                                                                                                                            >  1:270864/1‑299 (MQ=255)             gTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCATTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCAAGCCCGCt                                                                                                                                                                                                                                                                            >  2:518724/1‑298 (MQ=255)                       gATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAacac                                                                                                                                                                                                                                                                <  1:331342/300‑1 (MQ=255)                        gttcggggCAATGCGTTGAACAATCTTTAGAAACAGCGGGCCTATCTGTCAATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTAAATACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAACTGACTTCACCGATGCGGTCATTGATAGCGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACAcc                                                                                                                                                                                                                                                               <  2:269333/295‑1 (MQ=255)                        gttCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCACGGCCCGCTGGAACGTAACAcc                                                                                                                                                                                                                                                               >  1:237309/2‑300 (MQ=255)                        aTTCTGGTCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGTTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCACCCCCGGCCCGCTGGAACGTAACAcc                                                                                                                                                                                                                                                               <  2:269200/300‑1 (MQ=255)                        aTTCTGGGCAATGCGTGGAACAATCTTTAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACAcc                                                                                                                                                                                                                                                               <  2:268555/300‑1 (MQ=255)                        aTTCTGGGCAATGCGGGGGACAATTTTGGGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGTTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCTGCTACATGGCCGGGCGTTATCTGATTGAGCGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAATGTAACAcc                                                                                                                                                                                                                                                               <  2:269343/300‑1 (MQ=255)                                    gCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCCGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCCAAAGCTGACTTCACAGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTCCATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGAGTCATCCAAGGCCCGCTGGAACGTAacaacagc                                                                                                                                                                                                                                                            >  2:300906/1‑286 (MQ=255)                                      ctgcgagatgtgtataagagacagggCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCAACGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCt                                                                                                                                                                                                                                                 >  1:256123/24‑300 (MQ=255)                                                cTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCAAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATNGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGtttt                                                                                                                                                                                                                                       >  1:471463/1‑300 (MQ=255)                                                   gagaAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGTTTTTATg                                                                                                                                                                                                                                    <  1:267771/300‑1 (MQ=255)                                                   gagaAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATg                                                                                                                                                                                                                                    <  1:267760/300‑1 (MQ=255)                                                   gagaAACAGCGGGCTTACCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGTTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATg                                                                                                                                                                                                                                    <  1:267696/300‑1 (MQ=255)                                                              ggCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGga                                                                                                                                                                                                                         <  1:569814/300‑1 (MQ=255)                                                                    tCTGTCGATGATGGCGCAAAAACGCTTCGATGGTCTGTTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGc                                                                                                                                                                                                                   <  2:111356/300‑1 (MQ=255)                                                                       gTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCgat                                                                                                                                                                                                                >  2:544085/1‑300 (MQ=255)                                                                              atgGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAg                                                                                                                                                                                                         <  1:637027/300‑1 (MQ=255)                                                                                 gcgcAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGt                                                                                                                                                                                                       >  1:153358/1‑299 (MQ=255)                                                                                                gATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGAt                                                                                                                                                                                        <  1:446017/299‑1 (MQ=255)                                                                                                    gTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGc                                                                                                                                                                                   <  1:13126/300‑1 (MQ=255)                                                                                                          tgGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGTTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGTCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGt                                                                                                                                                                              <  2:233919/299‑1 (MQ=255)                                                                                                          tgGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGt                                                                                                                                                                              <  1:78291/299‑1 (MQ=255)                                                                                                                     tCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGaa                                                                                                                                                                                                                                                                        >  2:442751/1‑198 (MQ=255)                                                                                                                     tCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGaa                                                                                                                                                                                                                                                                        <  1:442751/198‑1 (MQ=255)                                                                                                                     tCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTAGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGaa                                                                                                                                                                                                                                                                        <  1:442763/198‑1 (MQ=255)                                                                                                                     tCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTAGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGaa                                                                                                                                                                                                                                                                        >  2:442763/1‑198 (MQ=255)                                                                                                                          gTACCCAGAGCCGTTGCTGTCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATc                                                                                                                                                             <  1:261139/300‑1 (MQ=255)                                                                                                                          gTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATc                                                                                                                                                             <  1:574756/300‑1 (MQ=255)                                                                                                                          gTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCACCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATc                                                                                                                                                             <  1:261058/300‑1 (MQ=255)                                                                                                                          gTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACAGGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATc                                                                                                                                                             <  1:574740/300‑1 (MQ=255)                                                                                                                                 gagCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAacac                                                                                                                                                                                                                                                                <  1:508449/194‑1 (MQ=255)                                                                                                                                 gagCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAacac                                                                                                                                                                                                                                                                >  2:508449/1‑194 (MQ=255)                                                                                                                                      gTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCNCCGGCCCGCTGGAACGTAACACCGGCGCAGgcc                                                                                                                                                                                                                                                     >  2:188141/1‑200 (MQ=255)                                                                                                                                      gTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGgcc                                                                                                                                                                                                                                                     <  1:188141/200‑1 (MQ=255)                                                                                                                                       ttGCTGGCGATGCGGGAAGAGTATCGCCATATCCCAATGTTGGTCATGGACTGGGGGGGAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATTGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATcgcg                                                                                                                                                  <  2:287179/298‑1 (MQ=255)                                                                                                                                         gCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCAt                                                                                                                                              >  1:480102/1‑300 (MQ=255)                                                                                                                                                  gCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAAt                                                                                                                                     <  1:106787/300‑1 (MQ=255)                                                                                                                                                    tGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCNAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATc                                                                                                                                    <  2:510211/299‑1 (MQ=255)                                                                                                                                                                      tCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGcc                                                                                                                 >  1:191115/1‑300 (MQ=255)                                                                                                                                                                      tCCCAAGGGTGGTCATGGACTGGGTTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCTGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGcc                                                                                                                 <  2:95319/300‑1 (MQ=255)                                                                                                                                                                            tggtggTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTg                                                                                                           <  1:245048/300‑1 (MQ=255)                                                                                                                                                                             ggtggtTAGGGACTGGGGTTAAGCAAAAGCTGACTTCACCGATGCGGGCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCTGCGTCATCCCCGGCCCGCTGGAACGGAATACCGGCGCAGGCCGTCTGGCCGGTTTTATGAAGGCGATGTAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGGGCAGGTTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGc                                                                                                          <  1:1429/300‑1 (MQ=255)                                                                                                                                                                             ggtggtCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGc                                                                                                          <  1:260946/300‑1 (MQ=255)                                                                                                                                                                                      ggACTGGGGTGAAGCAANAGCTGACTTCACCGATGCNGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCNCTACTGCCGTCTTCtg                                                                                                 <  1:532138/300‑1 (MQ=255)                                                                                                                                                                                       gACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCAGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTAtgt                                                                                                >  2:601212/1‑300 (MQ=255)                                                                                                                                                                                              gTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCACGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTACCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTATTCTgtggt                                                                                             >  2:388526/1‑296 (MQ=255)                                                                                                                                                                                                   gcaagagttGACTTCACCGATGCGGTCATTGATAACGCGTTCGAATGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTTGCGATATc                                                                                    <  2:124061/292‑1 (MQ=255)                                                                                                                                                                                                   gCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATc                                                                                    <  2:426724/300‑1 (MQ=255)                                                                                                                                                                                                                          ggTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGTCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGtgc                                                             <  2:234594/300‑1 (MQ=255)                                                                                                                                                                                                                             cATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCAGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCAGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGtgctgc                                                          >  2:301476/1‑300 (MQ=255)                                                                                                                                                                                                                                                  cggcTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGt                                     >  1:626643/1‑300 (MQ=255)                                                                                                                                                                                                                                                     cTACATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTccc                                  >  1:108266/1‑300 (MQ=255)                                                                                                                                                                                                                                                       aCATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCTGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGc                                >  1:348254/1‑300 (MQ=255)                                                                                                                                                                                                                                                       aCATGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCTGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGAAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGc                                >  1:347340/1‑300 (MQ=255)                                                                                                                                                                                                                                                          tggacGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCAACCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATCTCATGGCAATGGGCGAACTTTGTGCTGCTGCTGAAATGGGCATGCGCGTcc                                   >  2:311121/5‑294 (MQ=255)                                                                                                                                                                                                                                                          tGGCCGGGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCCTCCACGGCCCGCTGGAACGTCACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGGTTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCCTGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGTCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGt                                     >  2:311141/1‑292 (MQ=255)                                                                                                                                                                                                                                                            gCCGTGCGTTATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGTCCCGCGGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGNAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGAt                           <  2:191115/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   gTTATCTGATTGAACGCGGTCATCGCGAAATCTGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGc                    <  2:541000/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    ttATCTGATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGGCTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTAATGCCGTCTTCTGTGGTGGCGATATCATGGAAATGGGAGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGGTGTTTCGCt                   >  2:256120/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          gATTGAACGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATc               >  1:525702/1‑298 (MQ=255)                                                                                                                                                                                                                                                                               aaCGCGGTCATCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCAGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTAt         >  1:318348/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                     gTCATCGCGAAATCGGCGTCATCCCCGGCCTGCTGGAACTTAACACCGTCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTTTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACg  <  2:391387/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                        GCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATCTGTCGATGATGGCGCAAAAACGCGTCGATGGTCTGCTGGTGATGTGTTCTGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGGTTATGATAACG  >  NC_000913/1738097‑1738672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑