Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,610,440	C→T	E81K (GAA→AAA)	uxaB ←	tagaturonate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,610,440	0	C	T	100.0%	177.2 / NA	56	E81K (GAA→AAA)	uxaB	tagaturonate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/34);  total (22/34)

CCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAACCTGTTTGAATTGGTACGAC  >  NC_000913/1610151‑1610729                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                  ccATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCa                                                                                                                                                                                                                                                                                         >  1:387226/1‑300 (MQ=255)   aTTGTAGTCAATCAACTCACACTGAATGATGATCCAACCGTTAGCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCAtt                                                                                                                                                                                                                                                                                       <  2:238507/300‑1 (MQ=255)    gtttAGTCAATCAACTCACACGGAATGATAATCCAACCTTTATCCAGCGCACCGTGGAAATGGCTAAAGCGTTCGAACAGCAGACTGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGTTTTCCCCTTCTCATTc                                                                                                                                                                                                                                                                                      <  2:236009/297‑1 (MQ=255)                     acacGTAATGATGATCCAACCTTTATCCAGCACACCGTTGAAATGGCTAAAGCGTTCGAACAGAAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAAtgg                                                                                                                                                                                                                                                                      <  2:379573/299‑1 (MQ=255)                     acacGGAATGATGATCCAACCCCTGGTCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAAtggt                                                                                                                                                                                                                                                                     <  2:579523/300‑1 (MQ=255)                     acacGGAATGAGGATCCCACCTTTATCCATCGCACCGGTGAAATTGCTAGAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCAGCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTTCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACACTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAAtgg                                                                                                                                                                                                                                                                      <  2:307392/299‑1 (MQ=255)                     acacGGAATGACAATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCGTACCGCTGGCGCGTCATCGAATTTATCACCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTGGAGAAAACAAAGCGCATTTCCGGGTTGTGCACCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTGACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAAtggt                                                                                                                                                                                                                                                                     <  2:579541/300‑1 (MQ=255)                     acaaggAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGGAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTGCTCATTCAGGCCACGGagaaggg                                                                                                                                                                                                                                                                      <  2:307213/295‑7 (MQ=255)                       acGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAAtggt                                                                                                                                                                                                                                                                     <  2:43759/298‑1 (MQ=255)                          gAATGATAATCCAACCTTCATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGGACAGCAGACTGGTCAGTTTTGCCGGAGAGCTTACCGCTGGTGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCGTCGGTGGTGGGAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTTATTCAGGCCACGGATAAtggt                                                                                                                                                                                                                                                                     <  2:43745/295‑1 (MQ=255)                            atgatgATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTAc                                                                                                                                                                                                                                                              <  2:101760/300‑1 (MQ=255)                            atgatgATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTAc                                                                                                                                                                                                                                                              <  1:30443/300‑1 (MQ=255)                                 gatCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGAcc                                                                                                                                                                                                                                                         <  2:369065/300‑1 (MQ=255)                                 gatCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGAcc                                                                                                                                                                                                                                                         <  1:336349/300‑1 (MQ=255)                                      aatcTTTATCCAGCGCACCGTTGAAATGGCTAAGGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCTCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATcc                                                                                                                                                                                                                                                     <  2:597798/296‑1 (MQ=255)                                      aaCCCTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTGAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATcc                                                                                                                                                                                                                                                     <  1:186156/299‑1 (MQ=255)                                        ccTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTAATCCAGGCCACGGATAATGGTGGTGTACAGACCACCCTGc                                                                                                                                                                                                                                                  >  2:122984/1‑300 (MQ=255)                                            tATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGc                                                                                                                                                                                                                                              <  2:614149/300‑1 (MQ=255)                                                         gTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGAcggcg                                                                                                                                                                                                                                  <  1:305031/299‑1 (MQ=255)                                                                 ggCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGaa                                                                                                                                                                                                                          >  1:611400/1‑299 (MQ=255)                                                                                       aCGGGTCAGTTTTGCCTGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAATAACGAc                                                                                                                                                                                                   <  2:245237/300‑1 (MQ=255)                                                                                                    gCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTATAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTc                                                                                                                                                                                      <  2:180252/300‑1 (MQ=255)                                                                                                    gCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCNTTTCCGGGTTGTGCGCCATTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTc                                                                                                                                                                                      <  2:506692/300‑1 (MQ=255)                                                                                                     ccGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAATTTCAATCGGACGAACAACGACCACGCCAGAATTCa                                                                                                                                                                                     >  1:210705/1‑300 (MQ=255)                                                                                                         aTAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGTTAGCTGATACCCTCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTGCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATc                                                                                                                                                                                 <  2:61593/300‑1 (MQ=255)                                                                                                           aGCTTACCACTTGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCgg                                                                                                                                                                               <  2:365486/300‑1 (MQ=255)                                                                                                                   gCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCTGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAAGTCAGATCGGTGTGCTc                                                                                                                                                                        <  2:405970/299‑1 (MQ=255)                                                                                                                   gCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCa                                                                                                                                                                       <  1:240030/300‑1 (MQ=255)                                                                                                                           gTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGga                                                                                                                                                                <  2:387226/299‑1 (MQ=255)                                                                                                                                  aaTTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGAtt                                                                                                                                                        >  1:623266/1‑300 (MQ=255)                                                                                                                                             cGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTcaac                                                                                                                                             >  1:200471/1‑300 (MQ=255)                                                                                                                                               cATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTcaacaa                                                                                                                                           >  1:372997/1‑300 (MQ=255)                                                                                                                                               cATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCAAGTcaacaa                                                                                                                                           >  2:76863/1‑300 (MQ=255)                                                                                                                                                                 cTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTAc                                                                                                                         >  2:112551/1‑300 (MQ=255)                                                                                                                                                                 cTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTAc                                                                                                                         >  1:574240/1‑300 (MQ=255)                                                                                                                                                                         gtgtTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCa                                                                                                                 >  1:410469/1‑300 (MQ=255)                                                                                                                                                                                               tttCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGAt                                                                                           >  1:240147/1‑300 (MQ=255)                                                                                                                                                                                               tttCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCAACAAACTGAATGATACGTTCTGGAt                                                                                           >  2:138861/1‑300 (MQ=255)                                                                                                                                                                                                           gcgcCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCgg                                                                               <  1:345994/300‑1 (MQ=255)                                                                                                                                                                                                                aGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGaaaa                                                                          >  2:149979/1‑300 (MQ=255)                                                                                                                                                                                                                aGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTAATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCAAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGaaaa                                                                          >  2:159202/1‑300 (MQ=255)                                                                                                                                                                                                                                 gTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAgtg                                                          <  1:27209/299‑1 (MQ=255)                                                                                                                                                                                                                                        cTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTcaca                                                  >  2:601931/1‑300 (MQ=255)                                                                                                                                                                                                                                        cTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGAAGATTTAGTGTTTTcaaa                                                  >  2:147197/1‑298 (MQ=255)                                                                                                                                                                                                                                                     tttCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCAAAATGGGTTCCCTTc                                     >  1:516055/1‑300 (MQ=255)                                                                                                                                                                                                                                                        cACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGa                                  >  1:493806/1‑300 (MQ=255)                                                                                                                                                                                                                                                          cGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGAtt                                >  1:23025/1‑300 (MQ=255)                                                                                                                                                                                                                                                            aTTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGNCCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAg                              <  1:122984/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    aGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCATGGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACaa                      <  2:355548/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    aGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACaa                      <  1:35688/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    aGCGAATCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGAGCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACaa                      <  2:436374/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        aaTCAGACGCGCGTCGCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTNTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAACCt                   >  1:129759/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                       gCTGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAACCTGTTTGAATTGGTAc     >  2:59936/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                         tGACCGCTTTCCCCTTCTCATTCAGTCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAACCTGTTTGAATTGGTACGAc  <  2:313783/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                         tGACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAACCTGTTTGAATTGGTACGAc  <  1:482112/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                          gACCGCTTTCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCATTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGTAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCTGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAACCTGTTTGAATTGGTACGAc  <  2:67585/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                  CCATTGTAGTCAATCAACTCACACGGAATGATGATCCAACCTTTATCCAGCGCACCGTTGAAATGGCTAAAGCGTTCGAACAGCAGACGGGTCAGTTTTGCCGGATAGCTTACCGCTGGCGCGTCATCGAATTTATCGCCCGCATGGTAGCTGATACCCGCTTCGGTGGTGTTAGAGAAAACAAAGCGCATTTCCGGGTTGTGCGCCAGTTTCAGGAATTCATCGTATTCACTGTAGACGCTGATTTCACGATTAACCGAGCGAATCAGACGCGCGTCGCTGACCGCTTCCCCCTTCTCATTCAGGCCACGGATAATGGTGGTGTACAGACCATCCTGCGTGCTCAGTGACGGCGGGAATGAAGTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGATTTAGTGTTTTCACAATGGGTTCCCTTCTGATTAGTCATACAACCTGTTTGAATTGGTACGAC  >  NC_000913/1610151‑1610729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑