Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,992	A→T	N271Y (AAT→TAT)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,992	0	A	T	100.0%	293.0 / NA	88	N271Y (AAT→TAT)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (39/49);  total (39/49)

ACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAA  >  NC_000913/1301698‑1302276                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                              aCCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAAc                                                                                                                                                                                                                                                                                          <  2:554103/300‑1 (MQ=255) aCCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAAc                                                                                                                                                                                                                                                                                          <  1:363826/300‑1 (MQ=255)      aaGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTNTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTATGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCAt                                                                                                                                                                                                                                                                                     >  2:207664/1‑300 (MQ=255)      aaGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCAt                                                                                                                                                                                                                                                                                     >  1:563291/1‑300 (MQ=255)      aaGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTATGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCAt                                                                                                                                                                                                                                                                                     >  2:549543/1‑300 (MQ=255)       aGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAGCCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCAt                                                                                                                                                                                                                                                                                     >  1:248935/1‑299 (MQ=255)         tGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGcc                                                                                                                                                                                                                                                                                  <  1:649958/300‑1 (MQ=255)                            tataAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGaa                                                                                                                                                                                                                                                                <  1:362321/299‑1 (MQ=255)                            tataAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTAGCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGAGTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCataa                                                                                                                                                                                                                                                                <  2:291694/299‑4 (MQ=255)                                   tACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGaaaa                                                                                                                                                                                                                                                        >  1:412870/1‑300 (MQ=255)                                          tCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGAt                                                                                                                                                                                                                                                 >  2:533592/1‑300 (MQ=255)                                             cccATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCNCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATcc                                                                                                                                                                                                                                               >  1:127197/1‑299 (MQ=255)                                                aTCAAATTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAGAATGGACCCAGCCTGGTAGTATCGTAACCAACGGTGCCGATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑ACAAAAACCGTTATTAAACAGGTAAACTATTTGCCTATTGCTTCTGAAGTTGCCGATGTCAAACGCTACCGTAGTGGTGAAATCGACACGACATATAACAGCCTGCCGATCTACTTGTTcacgaggccgagaaagaagatccagga                                                                                                                                                                                                                                           >  2:6581/1‑274 (MQ=255)                                                            ggTGCCAAAATCCGCTATCTAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTGAAAAGATTNGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTTG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACg                                                                                                                                                                                                                                <  2:511960/299‑1 (MQ=255)                                                            ggTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGTTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCTACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACg                                                                                                                                                                                                                                <  2:184101/299‑1 (MQ=255)                                                             gTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGtt                                                                                                                                                                                                                              >  1:327667/1‑300 (MQ=255)                                                                  aaaaGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGTTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AANAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATc                                                                                                                                                                                                                          <  2:197997/299‑1 (MQ=255)                                                                  aaaaGCCGCTAGCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AANAAC‑GCGAAAACCGTTATTAATCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGACCCCGGACGAAGTTCACGTTGATc                                                                                                                                                                                                                          <  2:197969/299‑1 (MQ=255)                                                                  aaaaGCCACTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTTGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AANAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCGTGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATc                                                                                                                                                                                                                          <  2:208707/299‑1 (MQ=255)                                                                         gCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGTTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAcc                                                                                                                                                                                                                    <  2:64706/298‑1 (MQ=255)                                                                         gCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAcc                                                                                                                                                                                                                    <  2:302525/298‑1 (MQ=255)                                                                         gCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCNATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCtg                                                                                                                                                                                                                  <  2:463886/300‑1 (MQ=255)                                                                           tATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAcc                                                                                                                                                                                                                    <  1:21626/296‑1 (MQ=255)                                                                           tATCGAGAAATTCGGAGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAcc                                                                                                                                                                                                                    <  2:63338/296‑1 (MQ=255)                                                                               gagaAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCTTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCAc                                                                                                                                                                                                             <  1:253573/299‑1 (MQ=255)                                                                               gagaAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCAc                                                                                                                                                                                                             <  1:254223/299‑1 (MQ=255)                                                                                agaAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGNTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCAc                                                                                                                                                                                                             <  2:473504/298‑1 (MQ=255)                                                                                agaAATGCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGTTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCAc                                                                                                                                                                                                             <  2:515869/298‑1 (MQ=255)                                                                                                    aCCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCGGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTaacaac                                                                                                                                                                                       <  2:301456/300‑1 (MQ=255)                                                                                                         gCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGCTCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCAGGACGAAGTTCACGTTGATCAATACCTGTGAACTTACTATTACGAAATTAACAACCAGaa                                                                                                                                                                                  >  2:12046/1‑300 (MQ=255)                                                                                                         gCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCCGAAGCTGAAAAAAGAGATCCAGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGaa                                                                                                                                                                                  >  2:605725/1‑300 (MQ=255)                                                                                                            ggcgaaTATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGCAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATCGCTTCTGAAGTTACCGATGTCAACCGCCACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGCCGAAGTTCACGTTGATCCATACCTGTGCAcgta                                                                                                                                                                                                          <  2:534883/269‑4 (MQ=255)                                                                                                              gTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGc                                                                                                                                                                             >  1:167147/1‑300 (MQ=255)                                                                                                                aaTATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGCAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATCGCTTCTGAAGTTACCGATGTCAACCGCCACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGCCGAAGTTCACGTTGATCCATACCTGTGCACTTa                                                                                                                                                                                                          <  2:534868/269‑1 (MQ=255)                                                                                                                          ccAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAt                                                                                                                                                                  >  1:586993/1‑299 (MQ=255)                                                                                                                                gTGCCTATACCTTAAGAGATTGGGTCGTAAACGAACTAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTTAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtg                                                                                                                                                           <  2:356111/300‑1 (MQ=255)                                                                                                                                gTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACNTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCCCGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAAcc                                                                                                                                                                               >  2:203433/1‑280 (MQ=255)                                                                                                                                gTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgt                                                                                                                                                            >  1:354982/1‑299 (MQ=255)                                                                                                                                gTGCCTATACCGTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTGGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCGATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtg                                                                                                                                                           <  2:648281/300‑1 (MQ=255)                                                                                                                                 tGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgt                                                                                                                                                          >  1:597293/1‑300 (MQ=255)                                                                                                                                  gCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCAGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgt                                                                                                                                                          >  1:267773/1‑299 (MQ=255)                                                                                                                                  gCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCACGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGCTCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACTGCCATTCAAAGAtgtgcgtgt                                                                                                                                                          >  2:151586/1‑299 (MQ=255)                                                                                                                                     tataCCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgtgcg                                                                                                                                                       <  1:670/299‑1 (MQ=255)                                                                                                                                     tataCCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCAGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgtgcg                                                                                                                                                       <  1:360282/299‑1 (MQ=255)                                                                                                                                         ccTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCCGGTAACCTATTTGCCTCTTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACCGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCAGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTAc                                                                                                                                                    >  1:265543/1‑298 (MQ=255)                                                                                                                                         ccTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGACATTCAACGATGTGCGTGTGCGTAcc                                                                                                                                                   >  1:265615/1‑299 (MQ=255)                                                                                                                                         cattAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAAAGATGTGCGTGTGCGTAcc                                                                                                                                                   >  2:57700/3‑299 (MQ=255)                                                                                                                                           ttcaaaGATTGGGTCNTAAACGAACGAATCGTTCNTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGANGTGCGTGTGCGTACCg                                                                                                                                                  <  1:538677/295‑1 (MQ=255)                                                                                                                                            tAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAc                                                                                                                                               <  1:578698/300‑1 (MQ=255)                                                                                                                                                             aacgaacgAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTAGTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGAc                                                                                                                             <  2:12137/300‑1 (MQ=255)                                                                                                                                                             aacgaacgAAGCGTTCTTGAG‑CGCAGCCCGACCTACTGA‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTTAACCGCTACCGTAGTGTTGAAATCGACATGACGTATAACAGCATGCCGATTGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAGGTTCGCGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCATAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGAc                                                                                                                             <  2:114174/300‑1 (MQ=255)                                                                                                                                                                 acgctaTCGTTCTTGAANCGCAGCCCGACCTACTGGNAACAACTGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACCNGAAACTAGGTATGGAc                                                                                                                             <  1:536343/295‑1 (MQ=255)                                                                                                                                                                          ttCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCAtt                                                                                                                 >  1:431150/1‑299 (MQ=255)                                                                                                                                                                            cTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCATATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTNGTGGTGAAATCGACATGACGTATAACAGCATGNAGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCAGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGCTGTGCGTGTGCGTACAGCAC‑TGAAATTAGGTATGGAACGTGATATCGTTGt                                                                                                               >  2:486117/1‑299 (MQ=255)                                                                                                                                                                                     cAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGt                                                                                                      >  1:586586/1‑300 (MQ=255)                                                                                                                                                                                            aCCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAgc                                                                                                >  1:204855/1‑299 (MQ=255)                                                                                                                                                                                               tACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGTGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTCCTAAAGTGAAAGCGCa                                                                                             >  1:2095/1‑299 (MQ=255)                                                                                                                                                                                               tACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCGTTGTTAATAAAGTGAAAGCGCa                                                                                             >  1:2201/1‑299 (MQ=255)                                                                                                                                                                                                aCTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAgg                                                                                           <  2:431150/300‑1 (MQ=255)                                                                                                                                                                                                                aaaaCCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGcc                                                                             <  2:627495/300‑1 (MQ=255)                                                                                                                                                                                                                    ccGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTa                                                                           >  2:582072/1‑298 (MQ=255)                                                                                                                                                                                                                      gTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGt                                                                       <  1:605725/300‑1 (MQ=255)                                                                                                                                                                                                                      gTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCGATGGt                                                                       >  2:583851/1‑300 (MQ=255)                                                                                                                                                                                                                                aGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCAc                                                             <  1:112523/300‑1 (MQ=255)                                                                                                                                                                                                                                aGGTAACCTATTTGCCTATTGCTTCTGAAGCTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCAc                                                             <  1:112530/300‑1 (MQ=255)                                                                                                                                                                                                                                   tAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGt                                                          <  1:411890/300‑1 (MQ=255)                                                                                                                                                                                                                                     aCCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGANATTAACAACCAGAAACCGCCATTCAACGATGTNCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGtat                                                        <  2:478838/300‑1 (MQ=255)                                                                                                                                                                                                                                     aCCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGtat                                                        <  1:510029/300‑1 (MQ=255)                                                                                                                                                                                                                                        tatgtgtcTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGTTTACACTCCACCGTATAc                                                      <  2:248935/292‑1 (MQ=255)                                                                                                                                                                                                                                        tATTTGTCTATTGCTGCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGACCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCTCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAATTGAAAGCGCAGTGCAACATGCCCGCCTATGGTTACACTCCACCGTATAc                                                      <  2:363929/299‑1 (MQ=255)                                                                                                                                                                                                                                        tATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCTTATTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGACCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATAc                                                      <  2:2201/299‑1 (MQ=255)                                                                                                                                                                                                                                        tATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATAc                                                      <  2:2095/299‑1 (MQ=255)                                                                                                                                                                                                                                        tATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATAc                                                      <  1:152469/299‑1 (MQ=255)                                                                                                                                                                                                                                        tATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCCTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATAc                                                      <  2:563291/299‑1 (MQ=255)                                                                                                                                                                                                                                           ttGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGAt                                                  <  1:207664/300‑1 (MQ=255)                                                                                                                                                                                                                                                         tGAAGTTACCGACGTCAACCGCTACCTTAGTGGTGAAATCGACATGACGTATAACAGCATGCCAATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAcc                                                                                                                                                                                                                    <  2:214245/125‑1 (MQ=255)                                                                                                                                                                                                                                                               tACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGcc                              <  1:549543/300‑1 (MQ=255)                                                                                                                                                                                                                                                               tACCGATGCCAACCGCTACCGTATTAGTGAAATCGACATGACGTATAACAACATGCCGATTGAATTGTTCTAGAAGCTGAAAAAAGAAATCCCGGACGGAGTTCACGTTGATCCATACCTGTGCACTTACTATTATGAAATTAACAACCATAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGAc                                                                                                                             <  2:114777/205‑1 (MQ=255)                                                                                                                                                                                                                                                                aCCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACNAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGTGCAAAATTGACTCAGCCa                             >  2:468093/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   gATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAANGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGaa                          >  1:127186/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   gATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGTGCAAAATTGACTcagacaga                           >  2:71740/1‑299 (MQ=255)                                                                                                                                                                                                                                                                              cTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCt                >  1:374846/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                aCCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTCATAAAGTGAAAGCGCAGGGCAACATGCCAGCCTATGGTTACATTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGa             >  2:68672/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                aCCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCAACGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGa             >  2:68779/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        ggtgAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTCGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCCTGAGCCAggga     >  2:185516/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                        ggtgAAATCGACATGACGTACCACAGCATGCCGATCGAATCGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCAAATTGATCCATACCTGTGCACCTACTATTAAGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGCAACTAGGTATGGACCGCGATATTATTGTTAATAAAGTGAAAGCGCCGGGCAACATGCCCGCCTa                                                                           >  2:185693/1‑230 (MQ=255)                                                                                                                                                                                                                                                                                           gAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGaaaaa  >  2:85466/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           gAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGACATTCAACGATGTGCGTGTGCGTACAGCAA‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGTGCAGGGCAACATGCACGCCTATGGTTACACTCCACCGTATACTGATGGTGCAAAATTGACTCAGCCAGAATGGTTTGGATGGAGCCAgaaagaa  >  2:87731/1‑294 (MQ=255)                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                              ACCTTAAGTGAACCCGTTCCGTACTTCTATAAATTACTTGTTCACCCATCAACTTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAA‑CGCAGCCCGACCTACTGG‑AACAAC‑GCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCAC‑TGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAA  >  NC_000913/1301698‑1302276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑