Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,100,070	G→A	C144Y (TGT→TAT)	ycdY →	chaperone protein YcdY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,100,070	0	G	A	100.0%	222.7 / NA	62	C144Y (TGT→TAT)	ycdY	chaperone protein YcdY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/29);  total (33/29)

TTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCT  >  NC_000913/1099790‑1100361                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                      ttGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCaa                                                                                                                                                                                                                                                                                     <  1:622455/299‑1 (MQ=255)     agagaaGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGATGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTgg                                                                                                                                                                                                                                                                                <  2:421826/296‑1 (MQ=255)       aaaaGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATTTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCTAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTgg                                                                                                                                                                                                                                                                                <  2:61858/298‑1 (MQ=255)          aGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGcc                                                                                                                                                                                                                                                                           <  2:230575/300‑1 (MQ=255)                      aCGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCAc                                                                                                                                                                                                                                                                >  1:381066/1‑299 (MQ=255)                      aCGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCACATGCAAacac                                                                                                                                                                                                                                                                >  1:381511/1‑299 (MQ=255)                      aCGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCATGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCAc                                                                                                                                                                                                                                                                >  1:381169/1‑299 (MQ=255)                           aGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCttt                                                                                                                                                                                                                                                          >  1:359648/1‑300 (MQ=255)                                      aTTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCATTTCTGGCGCAcc                                                                                                                                                                                                                                                >  2:258949/1‑299 (MQ=255)                                            aTGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCAc                                                                                                                                                                                                                                         <  1:566255/300‑1 (MQ=255)                                              gCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCATTTCTGGCGCACCATGGCAcc                                                                                                                                                                                                                                        >  1:341906/1‑299 (MQ=255)                                                   gttTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCATTTCTGGCGCACCATGGCACCGCTAAc                                                                                                                                                                                                                                  >  1:630507/1‑300 (MQ=255)                                                     ttATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAAccc                                                                                                                                                                                                                                <  1:424733/300‑1 (MQ=255)                                                               tGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCAt                                                                                                                                                                                                                      >  1:162210/1‑300 (MQ=255)                                                                 aaTGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTa                                                                                                                                                                                                                    >  2:618386/1‑300 (MQ=255)                                                                    gtgctgtgcCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGt                                                                                                                                                                                                                  >  1:601207/1‑299 (MQ=255)                                                                         gtgcCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACACATTTCTGGCGCACCATGGCCCCGCTAACCCGTGATGTAATTCGTGCAAt                                                                                                                                                                                                             >  2:180497/1‑299 (MQ=255)                                                                          tgcCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAAtgt                                                                                                                                                                                                           <  2:233257/300‑1 (MQ=255)                                                                                 atatCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGAc                                                                                                                                                                                                      <  2:359648/298‑1 (MQ=255)                                                                                         gcgcATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGgaa                                                                                                                                                                                             >  1:624772/1‑299 (MQ=255)                                                                                         gcgcATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCATTGGCAAAGTGGAGGCCCATGCCACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGgaa                                                                                                                                                                                             >  2:351082/1‑299 (MQ=255)                                                                                         gcgcATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCATGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGgaa                                                                                                                                                                                             >  2:627518/1‑299 (MQ=255)                                                                                                               ggaagcggaagTGCGCGCTTTTCTTTCTGAGCGATGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAgtgt                                                                                                                                                                     <  2:636221/299‑1 (MQ=255)                                                                                                                gaagcggaagTGCGCGCTTTTCTT‑‑TGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATc                                                                                                                                                                 <  1:224978/300‑1 (MQ=255)                                                                                                                 aagcggaagTGTGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCACTGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGAt                                                                                                                                                                  >  2:218463/1‑300 (MQ=255)                                                                                                                                 ttttCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGNCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAAc                                                                                                                                                  <  1:139133/300‑1 (MQ=255)                                                                                                                                        tctGAGCGAGGGATGCNCCATTAGGGCGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAGCATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGATNGTCTGAAGAATAAgtg                                                                                                                                                                      <  1:531006/280‑1 (MQ=255)                                                                                                                                                         ccATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATNTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATttgttgtt                                                                                                                             >  1:139655/1‑299 (MQ=255)                                                                                                                                                         caaTTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCATGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATCATttgttgtt                                                                                                                             >  2:17742/3‑299 (MQ=255)                                                                                                                                                          cATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGAGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGc                                                                                                                           >  2:568762/1‑300 (MQ=255)                                                                                                                                                                                gATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAGTGTGTGCTCGAtct                                                                                                       >  1:178330/1‑300 (MQ=255)                                                                                                                                                                                gATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGAtct                                                                                                       >  1:173993/1‑300 (MQ=255)                                                                                                                                                                                      aTCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCtcattc                                                                                                  <  1:31197/299‑1 (MQ=255)                                                                                                                                                                                      aTCGGCACATTGCTGCTCGCAGCGTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGCTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCtcattc                                                                                                  <  1:640242/299‑1 (MQ=255)                                                                                                                                                                                          gCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGc                                                                                             >  1:142754/1‑300 (MQ=255)                                                                                                                                                                                            acaTTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAGGCACGGTAAACACTATTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTACAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAAc                                                                                                                                                  <  2:20350/245‑1 (MQ=255)                                                                                                                                                                                               ttGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGt                                                                                        >  2:635920/1‑300 (MQ=255)                                                                                                                                                                                                      tCGCAGCTTCCTGGCTNGAAGATCAGTCAACGGAAN‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTNATGGCCGCGTTTTCTg                                                                                  <  1:544742/299‑1 (MQ=255)                                                                                                                                                                                                          aGCTTCCTGGCTTGAAGATCAGTCAACGGAAG‑ATGAAAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACTCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGAGGCCATTAGTGCAATGTTGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCtcattc                                                                                                  <  2:134108/279‑1 (MQ=255)                                                                                                                                                                                                           gCTTCCTTGCTGGAAGATCAGTAAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTTTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCAGGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTTGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTTTGATCTACGTCACTCATAACTTCAACGGATAATTTTTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCa                                                                            <  2:353227/300‑1 (MQ=255)                                                                                                                                                                                                           gCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCa                                                                            <  1:218463/300‑1 (MQ=255)                                                                                                                                                                                                                      ggAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCAt                                                                  >  2:341560/1‑299 (MQ=255)                                                                                                                                                                                                                       gAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATA‑GTGTGATCTACGTCAATCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGaa                                                               >  2:184628/1‑300 (MQ=255)                                                                                                                                                                                                                           aTCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGCGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACAt                                                            <  2:524190/300‑1 (MQ=255)                                                                                                                                                                                                                                tCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTtc                                                       <  2:548419/300‑1 (MQ=255)                                                                                                                                                                                                                                  aaaggAAG‑ATGAAAACGAAACACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGcc                                                                                                                                                                                                                                                                           <  2:79222/85‑1 (MQ=255)                                                                                                                                                                                                                                         g‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTAtt                                               >  1:227478/1‑299 (MQ=255)                                                                                                                                                                                                                                             gagaGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCaa                                           <  1:526932/300‑1 (MQ=255)                                                                                                                                                                                                                                               gagCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATc                                         <  2:347418/300‑1 (MQ=255)                                                                                                                                                                                                                                                        aCTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAATGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACAgg                                 <  1:493184/299‑1 (MQ=255)                                                                                                                                                                                                                                                         cTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACAGGCa                               >  1:627180/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   tGTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCgg                     <  2:19083/300‑1 (MQ=255)                                                                                                                                                                                                                                                                   ggTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCgg                     <  2:177003/299‑1 (MQ=255)                                                                                                                                                                                                                                                                    gTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGANGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGAGGCATGAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGt                    >  2:464111/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    gTTCAGTGAGTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCggc                    >  1:177003/1‑299 (MQ=255)                                                                                                                                                                                                                                                                           gAGTATCTGTTACCCTGGTATGGTGCGTTCCTGGGCAAATTGGAGGCCCATGCAACCACGCCTTTCGGGCGCACCATGGCACCGCTAACCCACGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTTAATAATGAGTGTGATCTACGTCACTCATAACTGCAACGCATAATTTGTTGTTGCATAAAATGTGTGCTCGACCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTg                                              <  2:541654/267‑1 (MQ=255)                                                                                                                                                                                                                                                                             gTATCTGTTACCCTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTACGGTATCTGGGGa           >  1:93393/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              tATCTGTTACCCTGGTATGGTGCGTTCCTTTGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTNAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGTGGa           <  2:200866/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                       cccTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACAGGTATTCTCTCCGGTATCTGGGGATGGGTGGCt  >  2:420768/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                       cccTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCt  >  2:417262/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                       cccTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCt  >  1:414331/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                       cccTGGTATGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTATCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCt  >  2:418002/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                      TTGCAGAAAAGCTGTGATATGACGCAAGTCTCTGCCGATTACAATGCGTTGTTTATCGGCGATGAATGTGCTGTGCCGCCATATCGTAGCGCATGGGTTGAGGGCGCGACGGAAGCGGAAGTGCGCGCTTTTCTTTCTGAGCGAGGGATG‑‑CCATTAG‑‑CGGATACGCCAGCCGATCACATCGGCACATTGCTGCTCGCAGCTTCCTGGCTGGAAGATCAGTCAACGGAAG‑ATGAGAGCGAAGCACTGGAAACACTGTTCAGTGAGTATCTGTTACCCTGGTGTGGTGCGTTCCTTGGCAAAGTGGAGGCCCATGCAACCACGCCTTTCTGGCGCACCATGGCACCGCTAACCCGCGATGCCATTAGTGCAATGTGGGACGAGCTGGAAGAAGAT‑‑TCTGAAGAATAAGTGTGATCTACGTCACTCATAACTGCAACGGATAATTTGTTGTTGCATAAAATGTGTGCTCGATCTCATTCATGGCCGCGTTTTCTGCTATCATGCGCGGCATGAACATACTTCTCTCTATTGCAATCACAACAGGCATTCTCTCCGGTATCTGGGGATGGGTGGCT  >  NC_000913/1099790‑1100361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑