Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	67,259	G→A	A360V (GCA→GTA)	araA ←	L‑arabinose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	67,259	0	G	A	98.7%	246.0 / ‑6.5	79	A360V (GCA→GTA)	araA	L‑arabinose isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (34/44);  minor base C (1/0);  total (35/44)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATCGCCGAGATCAATCAAGCTGGCGACAATCGCTGGGCCGGTTTGGGTATTGAAGATCAGGCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTGCGGCGATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCAGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCC  >  NC_000913/66969‑67553                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                       gcgCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCACTACCGAGATGTTGAACGTCGAGGATCGGTTTCTCTTCTacggcaat                                                                                                                                                                                                                                                                                                 >  2:622813/3‑295 (MQ=255) gcgCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATc                                                                                                                                                                                                                                                                                                >  2:392431/3‑299 (MQ=255)      tGGCTGAAGACGGTATNGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACNTCGAGGATCGGTTTCTCTNCTACGGCAATCGAc                                                                                                                                                                                                                                                                                             >  1:549249/1‑297 (MQ=255)        gCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGCGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGACGAGATCAATCAGGCTGTCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCAACAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCa                                                                                                                                                                                                                                                                                        >  1:158335/1‑300 (MQ=255)        gCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGTCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCa                                                                                                                                                                                                                                                                                        >  1:158086/1‑300 (MQ=255)        gCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCAACAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCCATCAACGGGCa                                                                                                                                                                                                                                                                                        >  2:389670/1‑300 (MQ=255)             aGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCTAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACtt                                                                                                                                                                                                                                                                                   <  2:280545/300‑1 (MQ=255)             aGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTAAGGCAATCGACGGGCAGACtt                                                                                                                                                                                                                                                                                   >  2:555361/1‑300 (MQ=255)                  gTATGGTGCGCTCCACCGGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACA‑GCGCATTCGCCACCGGTAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCGTACGGTAACGATCGCCTAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCTAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAg                                                                                                                                                                                                                                                                               <  2:175447/299‑1 (MQ=255)                         gcgcgcCACCAGCGAGGAGCCACGCTTCGTAAGCAGTTGGCAGATCCGGGTGCGCTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGNCGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAACCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATggg                                                                                                                                                                                                                                                                       <  2:212380/300‑1 (MQ=255)                             gcCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGc                                                                                                                                                                                                                                                                    <  1:411816/299‑1 (MQ=255)                              ccaccaGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCNCCGTGTCGATACAGTTAACCAGCAGACGGTAANGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTACAGCATATGGGAGcc                                                                                                                                                                                                                                                                   >  2:486266/1‑299 (MQ=255)                                   aGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCTAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGc                                                                                                                                                                                                                                                               <  2:285812/298‑1 (MQ=255)                                        ggATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGt                                                                                                                                                                                                                                                        <  1:44682/300‑1 (MQ=255)                                         gATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGCGATCACTCAGGCTGGCGACAATCGATGGACCGGTTTGGGTATTAAAGATCAGTCGAGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTAGAGGATCGGTTTCTATTCTACGGCAATCGACGGGCAGACTTCCAGAATATGGGAGCCGAGCACCa                                                                                                                                                                                                                                                           >  2:163781/1‑296 (MQ=255)                                              aCGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTAc                                                                                                                                                                                                                                                  <  1:116876/300‑1 (MQ=255)                                                                        gtttGCGCTTTCCACA‑GCGCATTTTCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGGGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGTGCAGGATCGTCCTTACCACCAATACCTAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGtcc                                                                                                                                                                                                                        <  2:56461/298‑1 (MQ=255)                                                                        ggtcgtgacttCCACACNCGCATTCGCCACCGGCAGNTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGNAGTGATAGGTGTAGtc                                                                                                                                                                                                                         <  1:539371/291‑1 (MQ=255)                                                                        ggTTGCGCTTTCCACA‑GCTCGTTCGCCACCTGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCTAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGtcc                                                                                                                                                                                                                        <  2:349694/300‑1 (MQ=255)                                                                        ggTTGCGCTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTANCCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGtcc                                                                                                                                                                                                                        <  1:137332/300‑1 (MQ=255)                                                                        ggTTGCGCTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGtcc                                                                                                                                                                                                                        <  1:605447/300‑1 (MQ=255)                                                                        ggTTGCGCTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTAACACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGtc                                                                                                                                                                                                                         <  1:647743/299‑1 (MQ=255)                                                                        ggTTGCGCTTTCCACA‑GCGAATTCGTCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAATAATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGAACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTTTCGAAGTGATAGGTGTAGtcc                                                                                                                                                                                                                        <  2:113791/300‑1 (MQ=255)                                                                        ggTTGCGCGTTCCACA‑GCGCATTCGCCACCGGCAGGTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCTAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGTGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGtcc                                                                                                                                                                                                                        <  1:430795/300‑1 (MQ=255)                                                                             cgcTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTCACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTAGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGAACCAGGTCATTCCCTTtct                                                                                                                                                                                                                                            >  2:549920/1‑275 (MQ=255)                                                                             cgcTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGAGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCAACAATACCGAGATGCTGAACGTCGAGGATCGGTTTATATTCCACGGCAATCGACAGGCAGACTTCCAGCATATGGGa                                                                                                                                                                                                                                                                      >  2:49803/1‑249 (MQ=255)                                                                              gcTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATNGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTAACTTTCTCGAAGTGATAGGTGTAGTCCTCCATa                                                                                                                                                                                                                  >  1:469999/1‑300 (MQ=255)                                                                              gcTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATa                                                                                                                                                                                                                  >  1:105864/1‑300 (MQ=255)                                                                                 ttCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCAGGCAGGATCGTCCTTACCCCCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCCCTTCTACGGCAATCGACGGGCAGACTTCCAGCAGATGGGAGCAGAGCACCAGGTCATTACCCTTCTCGAAGTGACAGGTGCAGtactaaataaa                                                                                                                                                                                                                >  2:22711/1‑289 (MQ=255)                                                                                  tCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACNGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAagg                                                                                                                                                                                                              <  1:459001/300‑1 (MQ=255)                                                                                  tCCACA‑GCGCATTCACCACCGGCAGTTTCGGCAGTGAATGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGTGCAGGATCGTCCTTACCACCAATACCTAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAagg                                                                                                                                                                                                              <  2:17260/300‑1 (MQ=255)                                                                                              ttCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGccc                                                                                                                                                                                                   <  1:187934/300‑1 (MQ=255)                                                                                                              tCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATGTGGGAGCCGAGCACCAGGTCATTACCTTTCTAGAAGTGATGGGTGTAGTCCTCAATAAAGGAGGTGCAGCCCTGCAAAATGGTTGACa                                                                                                                                                                                   >  2:355537/1‑300 (MQ=255)                                                                                                                ggCAGGGAATGCGGTGTTTTCACCGTGTCGAGACAGTTAACCAGCAGACGGTAACGATCGACGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACAt                                                                                                                                                                                  <  1:75937/299‑1 (MQ=255)                                                                                                                    gggAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCTAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCt                                                                                                                                                                             <  1:64382/300‑1 (MQ=255)                                                                                                                    gggAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGTAGGTGCCGCCCTGCAGACCGGTTGACATCACCt                                                                                                                                                                             <  1:62593/300‑1 (MQ=255)                                                                                                                    gggAGTGCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCt                                                                                                                                                                             <  1:62582/300‑1 (MQ=255)                                                                                                                          gCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGTGCAGGATCGTCCGTACCACCAATACCTAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCatga                                                                                                                                                                       <  1:165686/300‑1 (MQ=255)                                                                                                                          gCGGTGTTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCTAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACTGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCatga                                                                                                                                                                       <  1:165429/300‑1 (MQ=255)                                                                                                                             gtgtTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGtcc                                                                                                                                                                                                                        <  1:209414/248‑1 (MQ=255)                                                                                                                             gtgtTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCNCCGGCAATCGACGGGCAGACTTCCCGCATATGGGAGCAGAGCAACAGGGCATTAACTTTATAGAAGTGGTAGGTGTAGtc                                                                                                                                                                                                                         >  2:209414/1‑247 (MQ=255)                                                                                                                               gtcttCACCGTGTCGATACAGTTAACCAGCAGACGGTAGCGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTACTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCat                                                                                                                                                                         >  2:245871/4‑293 (MQ=255)                                                                                                                               gtTTTCACCGTGTCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGa                                                                                                                                                                  >  1:400831/1‑300 (MQ=255)                                                                                                                                        gtgtCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCTAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGTAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAggg                                                                                                                                                           <  1:240140/298‑1 (MQ=255)                                                                                                                                        gtgtCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCTAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGggc                                                                                                                                                          <  1:158643/299‑1 (MQ=255)                                                                                                                                        gtgtCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGTAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGggc                                                                                                                                                          <  1:158979/299‑1 (MQ=255)                                                                                                                                        gtgtCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGggc                                                                                                                                                          <  1:158651/299‑1 (MQ=255)                                                                                                                                         tgtCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGTCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCTAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGggcgg                                                                                                                                                        <  1:580574/300‑1 (MQ=255)                                                                                                                                         tgtCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGaa                                                                                                                                                                 <  2:635674/291‑1 (MQ=255)                                                                                                                                          gtCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTAGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCCGCa                                                                                                                                                                                                                                                                             >  2:271168/1‑182 (MQ=255)                                                                                                                                          gtCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTAGGGCAGGATCGTCCTTACCAACAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGTATATGGGGGCAGAGCACCAGGTCATTACCTTTATCGAAGTGGGAGGTGTAGTCCTCCCTAAAGGAGGTGTCGCACTGCCGGCCGGTTACCATCACTTTCCTGATGCGAAGCAGgacggc                                                                                                                                                       >  2:269877/1‑300 (MQ=255)                                                                                                                                          gtCGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTCTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCTAGTATATGGGAGCCGCGCACCAGGTCATTACCTTTCTAGAAGTGATAGGTGTAGTCCTCCATCAGGGAGGTGCAGCCCTGCAGACCGGTTGGCATCACCTTCATGATGCGAAGCAGggcggc                                                                                                                                                       >  2:269342/1‑300 (MQ=255)                                                                                                                                            cGATACAGTTAACCAGCAGACGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGCGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGggcggc                                                                                                                                                       >  2:590715/1‑298 (MQ=255)                                                                                                                                                    ttAACCAGCAGACGGTANCGATCGCCGAGATCAATCNGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCANGGCGGCGGTTTTCCAg                                                                                                                                             <  1:546412/300‑1 (MQ=255)                                                                                                                                                                cGGTAACGATCGCCGAGATCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCAGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCAAGTCGCCTTCGccc                                                                                                                                 >  2:527812/1‑300 (MQ=255)                                                                                                                                                                              gagaTCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTGCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAAcc                                                                                                                   <  2:388562/300‑1 (MQ=255)                                                                                                                                                                               agaTCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCTAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAAccc                                                                                                                  <  1:152583/300‑1 (MQ=255)                                                                                                                                                                               agaTCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTCCGTTTATCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGCCATCACCTTAATGGTGCGAAGCGAGGCGGAGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTGAccc                                                                                                                  >  2:389284/1‑300 (MQ=255)                                                                                                                                                                               agaTCAATCAGGCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAAccc                                                                                                                  <  1:555361/300‑1 (MQ=255)                                                                                                                                                                                         ggCTGGCGACAATCGCTGGACCGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCa                                                                                                        >  2:452522/2‑300 (MQ=255)                                                                                                                                                                                              gCGACAATCGCTGGACCGGTTTGGGGATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACTTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGc                                                                                                   <  2:70720/300‑1 (MQ=255)                                                                                                                                                                                                          ggaccGGTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGa                                                                                       >  1:412573/4‑300 (MQ=255)                                                                                                                                                                                                               ggTTTGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGAAGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTAGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCAGTTGACATCAACTTCATGATGCGAAGCAGGGCGGCGATTTTCCAGTCGACTTCGCCCGCCAAGCCGTAACCCTGCTGCACCGGCCGCTGTACGGCCAGAcct                                                                                    >  2:175147/1‑297 (MQ=255)                                                                                                                                                                                                                 tttGGGTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACTGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGaa                                                                                <  2:454981/300‑1 (MQ=255)                                                                                                                                                                                                                   tGGGTATTGAAGATCAGTCGGGCAGGATCATCCTTACCACCAATACCTAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGc                                                                              <  2:126735/300‑1 (MQ=255)                                                                                                                                                                                                                   tGGGTATGGAAGAGCAGTCGTGCAGGACCGTCCCTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGTGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCACCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGc                                                                              <  2:126589/300‑1 (MQ=255)                                                                                                                                                                                                                    gggTATTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTATCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCAACTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCt                                                                             >  1:558729/1‑300 (MQ=255)                                                                                                                                                                                                                        aTTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCGGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGAACTGGAAGCTGtt                                                                          >  2:291654/1‑299 (MQ=255)                                                                                                                                                                                                                        aTTGAAGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTAGAAGTGATCGGTGTAGTCCCCCATAAAGGAGGTGCAGCCCTGCAGACTGGTTGACATCAACTTCATGATGCGAAGAAGGACGGCGGTTTTCCAGTCGCCTTCGCACGCACAGCCGTAGCCCTGCTGCATAAGACGCTGTCCGGACAGAACTGGAAGCTGttt                                                                         >  2:291531/1‑300 (MQ=255)                                                                                                                                                                                                                             agatcattcGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGGTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGCTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGAc                                                                    <  2:420868/292‑1 (MQ=255)                                                                                                                                                                                                                             aGATCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGTAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGAc                                                                    <  2:549249/300‑1 (MQ=255)                                                                                                                                                                                                                              gATCAGTCGGGCAAGATCGTCCTTACCACCAATACCTAGATGCGGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGTGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGAc                                                                    <  2:422693/299‑1 (MQ=255)                                                                                                                                                                                                                               aTCAGTCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCg                                                                  <  1:392431/300‑1 (MQ=255)                                                                                                                                                                                                                                       ggCAGGATCGTCCTTACCACCAATACCTAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGTAGACTTCCAGCATATGGGAGCCGAGCACCAGTTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGTAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATc                                                          <  2:87205/300‑1 (MQ=255)                                                                                                                                                                                                                                           ggATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCa                                                      >  1:483054/1‑300 (MQ=255)                                                                                                                                                                                                                                                   cTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAggtggt                                              >  1:576413/1‑300 (MQ=255)                                                                                                                                                                                                                                                        caccaATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGa                                         >  1:259922/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          gAACGTCGAGGATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCtt                       >  1:87554/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   ggATCGGTTTCTCTTCTACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGa              >  1:389306/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               cttctACGGCAATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCGGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCTGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATcc  >  2:403053/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                                                                       GTGCATGGCTGAAGACGGTATGGTGCGCGCCACCAGCGAGGATCCACGCTTCGGAAGCAGTTGGCAGATCCGGTTGCGCTTTCCACA‑GCGCATTCGCCACCGGCAGTTTCGGCAGGGAGTGCGGTGTTTTCACCGTGTCGATGCAGTTAACCAGTAGACGGTAACGATCGCCGAGATCAATCAAGCTGGCGACAATCGCTGGGCCGGTTTGGGTATTGAAGATCAGGCGGGCAGGATCGTCCTTACCACCAATACCGAGATGCTGAACGTCGAGGATCGGTTTCTCTTCTGCGGCGATCGACGGGCAGACTTCCAGCATATGGGAGCCGAGCACCAGGTCATTACCTTTCTCGAAGTGATAGGTGTAGTCCTCCATAAAGGAGGTGCCGCCCTGCAGACCGGTTGACATCACCTTCATGATGCGAAGCAGGGCGGCAGTTTTCCAGTCGCCTTCGCCCGCAAAGCCGTAACCCTGCTGCATCAGACGCTGTACGGCCAGACCAGGAAGCTGTTTCAGACCGTGCAAATCTTCAAAGGTGGTGGTGAACGCGTGGAAGCCACCTTGTTCCAGGAAACGCTTCATCC  >  NC_000913/66969‑67553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑