Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,392,997	G→A	V357V (GTC→GTT)	queG ←	epoxyqueuosine reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,392,997	0	G	A	100.0%	317.8 / NA	88	V357V (GTC→GTT)	queG	epoxyqueuosine reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/50);  total (38/50)

TGTTGTCTCTGACAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCGACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCGGTGCGTGTAGCGGCTTACGCGGGCTGAAATCCTCTTCTGTGGTGAGT  >  NC_000913/4392713‑4393288                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                     tgttgtCTCTGACAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑ttt                                                                                                                                                                                                                                                                                      >  1:147079/1‑300 (MQ=255)      tctcTGACAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGtct                                                                                                                                                                                                                                                                                 >  2:413457/1‑300 (MQ=255)              aaaCTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACTTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTAtt                                                                                                                                                                                                                                                                          <  2:306151/299‑1 (MQ=255)               aaCTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTtg                                                                                                                                                                                                                                                                        <  1:76002/300‑1 (MQ=255)               aaCTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTtg                                                                                                                                                                                                                                                                        <  2:619805/300‑1 (MQ=255)                actcgtggaaaCAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTtgc                                                                                                                                                                                                                                                                       <  1:230762/292‑1 (MQ=255)                aCTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAATTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTtgc                                                                                                                                                                                                                                                                       <  1:347194/300‑1 (MQ=255)                aCTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTTTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTtgc                                                                                                                                                                                                                                                                       <  1:64850/300‑1 (MQ=255)                aCTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTtgc                                                                                                                                                                                                                                                                       <  1:485519/300‑1 (MQ=255)                 ccgagatgtgtataagngacagcaGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCNGCTTCAACTATGCACGCANTTTCGTctctctgcttt                                                                                                                                                                                                                                                                        >  1:536160/23‑295 (MQ=255)                  tctTGTAAACAGAGTTATCCACAGCCTCAGACTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAACCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTtgc                                                                                                                                                                                                                                                                       <  1:12877/298‑1 (MQ=255)                     tGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACATATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTgcgc                                                                                                                                                                                                                                                                  >  2:608716/1‑300 (MQ=255)                      gTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCa                                                                                                                                                                                                                                                                 >  1:272531/1‑300 (MQ=255)                             gggTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCCTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTATCTATTTGCTGCGCAATCGccc                                                                                                                                                                                                                                                          >  1:331227/3‑300 (MQ=255)                             gagTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGccc                                                                                                                                                                                                                                                          >  1:264326/1‑300 (MQ=255)                             gagTTATCCACAGCCTAAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCAAGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTATCTATTTGCTGCGCAATCGccc                                                                                                                                                                                                                                                          >  1:330836/1‑300 (MQ=255)                                 tATCCACAGCCTCATGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTACTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGc                                                                                                                                                                                                                                                      <  2:449514/300‑1 (MQ=255)                                   tCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTTTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTa                                                                                                                                                                                                                                                    <  2:432719/300‑1 (MQ=255)                                    ccACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTAt                                                                                                                                                                                                                                                   >  2:444343/1‑300 (MQ=255)                                       caGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTAtgt                                                                                                                                                                                                                                                 >  1:426798/1‑299 (MQ=255)                                        atccTCAGGCTGTAATCTTAATTGCAAAGAAACTTCGCACTGTGAATAGTATTTTTTTAACCCATTGATAGGTAGGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATGCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCATGCGTCACGCGTTAACCCTTTTTCAATCACCCGAACCAGACACTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTGTTTGCTGTGTAATCGTTCACGCTATGTGc                                                                                                                                                                                                                                               <  2:110232/298‑1 (MQ=255)                                        aGCCTCAGGCTTTAATCTTAATTTCAAAGAAACTTCGCACTGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTAtgtg                                                                                                                                                                                                                                                <  2:105644/299‑1 (MQ=255)                                        aGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCATTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTAtgggc                                                                                                                                                                                                                                               <  1:382136/300‑4 (MQ=255)                                        aGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGc                                                                                                                                                                                                                                               <  2:54215/300‑1 (MQ=255)                                         gCCTCAGGCTGTAATCTTCATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCAGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCt                                                                                                                                                                                                                                              >  1:513978/1‑300 (MQ=255)                                         gCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCt                                                                                                                                                                                                                                              >  1:265981/1‑300 (MQ=255)                                         gCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCt                                                                                                                                                                                                                                              >  2:141848/1‑300 (MQ=255)                                         gCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTATCTATTTGCTGCGCAATCGCCCACGCTATGTGCt                                                                                                                                                                                                                                              >  1:266120/1‑300 (MQ=255)                                                  tGTAATCTTAATTTCAAATAAACTTCGCACGGTGAATAGTATTTCGTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTAGTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGc                                                                                                                                                                                                                                               <  2:110197/290‑1 (MQ=255)                                                   gTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAg                                                                                                                                                                                                                                    <  2:159545/300‑1 (MQ=255)                                                   gTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAg                                                                                                                                                                                                                                    <  1:430862/300‑1 (MQ=255)                                                   gTAATCTCAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGGTAAAAATTAAGATTCCGTTTGGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAAGTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCTTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTACTCATCGAGAAg                                                                                                                                                                                                                                    <  2:57972/300‑1 (MQ=255)                                                        cTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGNTTTTATTCGTCTTGAATTGCAATGCGTTTTTANTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGt                                                                                                                                                                                                                               >  2:497179/1‑300 (MQ=255)                                                                       aCTTCACACGGTGAATAAGATTCTTTGAACCTGCTGATAGATAAATTAAAAATTAATATTCCGTTTTTTCGAGTCGGTCGCTTGGCGATTTTATTCGTCTTGAGTGGCAATGCGTGTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCGTACGCTGTTTTTTCTGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCTAGAAGTGGGTGCTCACCTTTACGAc                                                                                                                                                                                                                <  2:172560/300‑1 (MQ=255)                                                                                 gTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCTTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTNGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAgcgc                                                                                                                                                                                                      <  2:499451/300‑1 (MQ=255)                                                                                           tttttttAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCTAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATc                                                                                                                                                                                            <  2:627796/300‑1 (MQ=255)                                                                                             tttttAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTNCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGt                                                                                                                                                                                          >  1:126448/1‑300 (MQ=255)                                                                                             tttttAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTNCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCAAGCGCTGTCAAAATCGt                                                                                                                                                                                          >  1:124994/1‑300 (MQ=255)                                                                                             tttttAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTATCTATTTNCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGt                                                                                                                                                                                          >  1:124965/1‑300 (MQ=255)                                                                                                       aTTGATAGATAAGCTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCATCTTGAATTGCAATGCGTTTTTATTTTCATTCACCAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTAAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCaa                                                                                                                                                                                >  2:349686/1‑300 (MQ=255)                                                                                                          gatagataAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAgtg                                                                                                                                                                             <  2:403312/300‑3 (MQ=255)                                                                                                           atagataAGTTAAAAATTAAGATTCCGTTTTGTCGATTCGATCACTTGACGATTTTATTCGTCGTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAggg                                                                                                                                                                             <  2:165024/299‑1 (MQ=255)                                                                                                           atagataAGTTAAAAATTAAGATTCCGTTTTGTCGATGCGATCACTTGACGATTTTATTCTTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAggg                                                                                                                                                                             <  2:403057/299‑1 (MQ=255)                                                                                                             agataAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTNTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCACAAGGGGCa                                                                                                                                                                          <  1:141848/300‑1 (MQ=255)                                                                                                              gataAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACACTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTATCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCAt                                                                                                                                                                         >  2:639354/1‑300 (MQ=255)                                                                                                                   gTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCt                                                                                                                                                                    >  1:363289/1‑300 (MQ=255)                                                                                                                   gTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTACAGCGCTGTCAAAATCGTTTAATCCCAAGGGGCATTGCCt                                                                                                                                                                    >  1:363671/1‑300 (MQ=255)                                                                                                                        aaaTTAAGATTCCGTTTTTTCGATTCGGTCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCATGCGGTAACCCTTTGTCAATCACCCGAACCAGACACTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCTAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAATGGGCATTGCCTAATGc                                                                                                                                                               <  2:185804/300‑1 (MQ=255)                                                                                                                        aaaTTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGc                                                                                                                                                               <  1:444343/300‑1 (MQ=255)                                                                                                                             aaGATTCCGTTTTTTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAACTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTACTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTGCATCCCAATGGGCATTGCCTAATGCTACgg                                                                                                                                                          <  2:186486/300‑1 (MQ=255)                                                                                                                               gATTCCGTTTTGTCGATTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATCCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCTAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCg                                                                                                                                                        <  2:446177/300‑1 (MQ=255)                                                                                                                                   ccGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGTGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCAAAGGGGCATTGCCTAATGCTACGGCGatat                                                                                                                                                    >  1:96924/1‑300 (MQ=255)                                                                                                                                     gTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTATCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCNTCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTa                                                                                                                                                  >  2:507701/1‑300 (MQ=255)                                                                                                                                        ttttcgagtcgaTCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGc                                                                                                                                               <  1:168907/297‑1 (MQ=255)                                                                                                                                        ttGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGc                                                                                                                                               <  1:232742/300‑1 (MQ=255)                                                                                                                                                       cTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTAATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTAAGCAGCCAACGCAGGTGa                                                                                                                                >  2:400146/1‑300 (MQ=255)                                                                                                                                                           aCGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCaa                                                                                                                            <  1:215187/300‑1 (MQ=255)                                                                                                                                                              aTTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCTAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATac                                                                                                                         <  2:451096/300‑1 (MQ=255)                                                                                                                                                                  tATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATacgac                                                                                                                      <  2:632725/299‑1 (MQ=255)                                                                                                                                                                       gTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCNATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATc                                                                                                                 >  2:197959/1‑299 (MQ=255)                                                                                                                                                                       gTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCAAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTATCTATTTGCTGCGCAATCGCCCACGCTCTGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCAAGCGCTGTCAAAATCGTTTCGTCCCGAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATc                                                                                                                 >  2:334278/1‑299 (MQ=255)                                                                                                                                                                            gAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCTGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGAt                                                                                                           >  1:523369/1‑300 (MQ=255)                                                                                                                                                                                        gTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGCATCGCCGATCCTTCCGTGACt                                                                                               >  2:583160/1‑300 (MQ=255)                                                                                                                                                                                        gTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGAc                                                                                                <  2:110465/299‑1 (MQ=255)                                                                                                                                                                                                     ttCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTtct                                                                                  >  1:427989/1‑300 (MQ=255)                                                                                                                                                                                                            gCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCt                                                                           >  1:449707/1‑300 (MQ=255)                                                                                                                                                                                                            gCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCAGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCt                                                                           >  1:111457/1‑300 (MQ=255)                                                                                                                                                                                                             cTGTGGATGAATCAGGCGTCAAGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAACTACA‑CTCCAACTATGTACGTA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCCTCGAGAAGTGGGTGCTCGCCTTTCCGACTTTCCAGCGCTGTCACAATCGTTTCATACAAAGGGGCATTGCCTAGTGCTACGGCGATATTATGCAGCAAAAGCATGTGAACAATACGAAGAAGCGAAGATCATTGCGTGAATTTTAAAAACTTCTCTTCGCta                                                                          >  2:169077/1‑299 (MQ=255)                                                                                                                                                                                                                   aTGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCATGCg                                                                    <  1:193181/300‑1 (MQ=255)                                                                                                                                                                                                                      aaTCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGa                                                                   >  2:636909/1‑298 (MQ=255)                                                                                                                                                                                                                      aaTCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAAGTGACCAATACGACGAATCGCCGATCCTTCAGTGACTTTTAAAAACTTATCTTCGCTCCAGGCGAAt                                                                 >  2:636985/1‑300 (MQ=255)                                                                                                                                                                                                                      aaTCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTCTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTAAGTGACTTGTAATAACTTCTCTTCGCTCCAGGCGAAt                                                                 >  2:636938/1‑300 (MQ=255)                                                                                                                                                                                                                              gTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTAAGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAAt                                                         >  2:329520/1‑300 (MQ=255)                                                                                                                                                                                                                              gTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTAGTCTATCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGAAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAAt                                                         >  2:329433/1‑300 (MQ=255)                                                                                                                                                                                                                                         aCCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCGGTg                                              <  2:456206/300‑1 (MQ=255)                                                                                                                                                                                                                                             tttttCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTATGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAATGTGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCAATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCATGCGAATAACTCAATGAGTTCCGGTGCgt                                           <  2:265981/299‑1 (MQ=255)                                                                                                                                                                                                                                              ttttCAATCACCCAAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAATGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCGGTGCgt                                           <  2:266120/298‑1 (MQ=255)                                                                                                                                                                                                                                                 tCAATCACCCGAACCATACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACNCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAATGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCGGTGCGTGTAGc                                      <  2:513978/300‑1 (MQ=255)                                                                                                                                                                                                                                                   aaTCACCCGAGCCAGACGCTGTTTGTTCGGCAATTACA‑CTTCAACTATTCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCTAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAATGTGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCGGTGCGTGTAGCgg                                    <  2:124994/300‑1 (MQ=255)                                                                                                                                                                                                                                                   aaTCACCCGAACCAGACTTTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCGGTGCGTGTAGCgg                                    <  2:124965/300‑1 (MQ=255)                                                                                                                                                                                                                                                   aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCGGTGCGTGTAGCgg                                    <  2:126448/300‑1 (MQ=255)                                                                                                                                                                                                                                                   aaTCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCATTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCGGTGCGTGTAGCgg                                    <  1:197959/300‑1 (MQ=255)                                                                                                                                                                                                                                                     tCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCGGTGCGTGTAGCGGCt                                  <  1:423510/300‑1 (MQ=255)                                                                                                                                                                                                                                                           gAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCGCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACTACTAATCGCCGATCCTTCCGTGACTTTTAAAAACTCCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCGGTGCGTTTAGCGGCTTAcgc                             <  1:332125/299‑1 (MQ=255)                                                                                                                                                                                                                                                                      tGTTTTGTCGGCAATTGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCGGTGCGTGTAGCGGCTTACGCGGGCTGAAAt                   <  2:264326/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                     tGCA‑CTTCAACTGTGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCGGTGCGTGTAGCGGCTTACGCGGGCTGAAATCCTCTTCTGTGGTGAGt  <  2:330836/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     tGCA‑CTTCAACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCGGTGCGTGTAGCGGCTTACGCGGGCTGAAATCCTCTTCTTTGGTGAGt  <  2:147079/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                     tGCA‑CTGCAACTATTCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCACTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCGGTGCGTGTAGCGGCTTACGCGGGCTGAAATCCTCTTCTGTGGTGAGt  <  2:331227/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                     TGTTGTCTCTGACAAACTCTTGTAAACAGAGTTATCCACAGCCTCAGGCTGTAATCTTAATTTCAAAGAAACTTCGCACGGTGAATAGTATTTTTTTAACCTATTGATAGATAAGTTAAAAATTAAGATTCCGTTTTGTCGAGTCGATCACTTGACGATTTTATTCGTCTTGAATTGCAATGCGTTTTTATTTTTATTCACAAGCTGTGGATGAATCAGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCTGTTTTTTCGGCAATTGCA‑CTTCGACTATGCACGCA‑TTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAACTTCTCTTCGCTCCAGGCGAATAACTCAATGAGTTCCGGTGCGTGTAGCGGCTTACGCGGGCTGAAATCCTCTTCTGTGGTGAGT  >  NC_000913/4392713‑4393288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑