Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,015,225	G→T	D725Y (GAC→TAC)	metE →	cobalamin‑independent homocysteine transmethylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,015,225	0	G	T	100.0%	214.7 / NA	61	D725Y (GAC→TAC)	metE	cobalamin‑independent homocysteine transmethylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (34/27);  total (34/27)

ACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCT  >  NC_000913/4014958‑4015520                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                        acacaAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGaa                                                                                                                                                                                                                                                                         >  1:589745/1‑300 (MQ=255) acacaAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCATGAAAACGCGCGGCTGGCCAGaa                                                                                                                                                                                                                                                                         >  1:589394/1‑300 (MQ=255)    caAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAAcc                                                                                                                                                                                                                                                                      >  1:311918/1‑300 (MQ=255)                cacaTGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACGTGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACt                                                                                                                                                                                                                                                           >  1:613798/1‑299 (MQ=255)                cacaTGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACt                                                                                                                                                                                                                                                           >  1:630154/1‑299 (MQ=255)                   aTGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCg                                                                                                                                                                                                                                                       <  1:507375/300‑1 (MQ=255)                        ttATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACAt                                                                                                                                                                                                                                                  <  1:375220/300‑1 (MQ=255)                                  ttCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAggcg                                                                                                                                                                                                                                        <  1:339008/300‑1 (MQ=255)                                                               gcTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGTAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATcc                                                                                                                                                                                                           <  1:160676/300‑1 (MQ=255)                                                               gcTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATcc                                                                                                                                                                                                           >  1:57065/1‑300 (MQ=255)                                                                   gACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAAc                                                                                                                                                                                                       <  1:526148/300‑1 (MQ=255)                                                                    aCGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTaaaactcaaac                                                                                                                                                                                                       >  2:102663/1‑292 (MQ=255)                                                                         gACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGggt                                                                                                                                                                                                  >  1:55161/1‑299 (MQ=255)                                                                           cGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGggtggt                                                                                                                                                                                               >  2:455243/1‑300 (MQ=255)                                                                                tcaCCATCGAAACCTCGCGTTCCGACATGGAGTTGCTTGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATac                                                                                                                                                                                          <  2:431677/300‑1 (MQ=255)                                                                                  aCCATCGAAACCTCGCGTTCCTACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATacc                                                                                                                                                                                         <  2:20184/299‑1 (MQ=255)                                                                                  aCCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGGGTCGTTTTAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCTAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATacc                                                                                                                                                                                         <  2:20855/299‑1 (MQ=255)                                                                                    cATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTNAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCAGTACTGTGGCCTGAAAACGCGCGGCTGGCAAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATaccacc                                                                                                                                                                                      >  2:466210/1‑300 (MQ=255)                                                                                    cATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCAGGCAGAGNGCCTGTGGGTCACCACGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATaccacc                                                                                                                                                                                      >  2:194249/1‑300 (MQ=255)                                                                                         aaaCCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCAACCGGTc                                                                                                                                                                                 >  2:145896/1‑300 (MQ=255)                                                                                         aaaCCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGAAATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCAGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGAGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAGTaccacacagtc                                                                                                                                                                                 >  2:149175/1‑294 (MQ=255)                                                                                                gcgTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTtctc                                                                                                                                                                          <  1:493053/300‑1 (MQ=255)                                                                                                   ttCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGANCATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCAtt                                                                                                                                                                       >  1:463575/1‑300 (MQ=255)                                                                                                        aCATGGAGTTGCTGTAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCACTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGc                                                                                                                                                                  <  2:531210/300‑1 (MQ=255)                                                                                                        aCATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATGATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCAGTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACatc                                                                                                                                                                  <  2:530959/300‑3 (MQ=255)                                                                                                                           gTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATAc                                                                                                                                                <  1:153233/299‑1 (MQ=255)                                                                                                                           gTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATAc                                                                                                                                                <  2:446622/299‑1 (MQ=255)                                                                                                                                aaGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGTTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCtt                                                                                                                                           <  1:302667/299‑1 (MQ=255)                                                                                                                                    gTTTGATTATCCAAGTGGGATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGGACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTTGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAGGATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAAcc                                                                                                                                      <  2:146625/300‑1 (MQ=255)                                                                                                                                    gTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCTTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCNAGAAACCCGCGCGGCACTGGCGAACATGGTGCNGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAAcc                                                                                                                                      <  2:482900/300‑1 (MQ=255)                                                                                                                                    gTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTTAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGGACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAAcc                                                                                                                                      <  2:435706/300‑1 (MQ=255)                                                                                                                                    gTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAAcc                                                                                                                                      <  1:488969/300‑1 (MQ=255)                                                                                                                                                  aTGAAATCGGTCTGGGCGTCTATTACATTCACTCGCCAAACGTACCAAGCGTGGAATGGATGGAAGCCTTGCTGAAGAAATCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCTGCACTGTCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGc                                                                                                                        <  2:336077/300‑1 (MQ=255)                                                                                                                                                           gTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTANTACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTgccagcca                                                                                                               >  1:128504/1‑300 (MQ=255)                                                                                                                                                            tCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCAt                                                                                                              <  1:398528/300‑1 (MQ=255)                                                                                                                                                                ggCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCAtcttc                                                                                                          >  2:537391/1‑300 (MQ=255)                                                                                                                                                                   gTCTATGACATTCACTNGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTNAAACCATTCCAGCATACGNTGCCAGCCATCTTCTGc                                                                                                       >  1:551811/1‑300 (MQ=255)                                                                                                                                                                     cTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCAGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCa                                                                                                      >  2:110352/1‑299 (MQ=255)                                                                                                                                                                      tacgACATTCACTCGCCAAACGTACCGAGCTTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAGCTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCACTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAg                                                                                                     <  2:73095/296‑1 (MQ=255)                                                                                                                                                                      tATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTTAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAg                                                                                                     <  2:73432/299‑1 (MQ=255)                                                                                                                                                                       aTGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATACAAACCGGGTGGTAATACCAACCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCAAGCCATTTTCTGCAGAt                                                                                                   >  2:46670/1‑300 (MQ=255)                                                                                                                                                                         gACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGc                                                                                                 >  2:603165/1‑300 (MQ=255)                                                                                                                                                                                  tCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGAt                                                                                         <  1:259893/299‑1 (MQ=255)                                                                                                                                                                                          cGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGc                                                                                >  2:599688/1‑300 (MQ=255)                                                                                                                                                                                          cGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTAAAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATCCGCTGCCAGCCATCTTCTGCAGATGTGGCCTGATAGCTAGGGc                                                                                >  2:295776/1‑300 (MQ=255)                                                                                                                                                                                                      ggAATGGATTGAAGCCTTGCTTAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTTGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGt                                                                    <  2:328150/300‑1 (MQ=255)                                                                                                                                                                                                       gAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCAGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGGTAGCTCGGGCGATAATCAGCGtt                                                                   >  2:37736/1‑300 (MQ=255)                                                                                                                                                                                                           ggATTGAATCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAat                                                               <  2:606230/300‑1 (MQ=255)                                                                                                                                                                                                           ggATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCATACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTG‑AGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAat                                                               <  1:209890/299‑1 (MQ=255)                                                                                                                                                                                                                                  gCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTacac                                        <  1:186642/300‑1 (MQ=255)                                                                                                                                                                                                                                       aaaaCGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCAAGCCATCTTCTGCCGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGCATGCATGCCCGGCGTCCGGGTACACGATaa                                   >  2:154534/1‑300 (MQ=255)                                                                                                                                                                                                                                               ttCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCNGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCt                           >  1:468540/1‑300 (MQ=255)                                                                                                                                                                                                                                                            ccTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTagc                >  2:597964/1‑298 (MQ=255)                                                                                                                                                                                                                                                            ccTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATAAGCGTTGCATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTagca               >  2:386184/1‑299 (MQ=255)                                                                                                                                                                                                                                                                      accccGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAGTACCAACCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTANACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAACCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAgcgc    >  2:205608/3‑300 (MQ=255)                                                                                                                                                                                                                                                                      accccGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTATGCAGATGCGGCATGATAGCTCGGGCGATAACCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAgc      >  2:525114/3‑298 (MQ=255)                                                                                                                                                                                                                                                                      accccGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCANTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAgcgc    >  2:469776/3‑300 (MQ=255)                                                                                                                                                                                                                                                                      accccGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTAATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCAAGCAATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGTCCGGCGTCCGGGTACACGATAATCTATGCTTTCGCATTAGCCGCCCGCAgcgc    >  2:355288/3‑300 (MQ=255)                                                                                                                                                                                                                                                                      accccGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGCCTTTTCTCATTCCAGCGACTTCCTCCCACCATAATGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATTTATGATTTCGAATTAGCAGCCAGCAgcgc    >  2:524875/3‑300 (MQ=255)                                                                                                                                                                                                                                                                      accccGTACTATGGCCTGCAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCAACCGGTCTTTTCTCATTACAGCGAATTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCAGCACGATAGCTCGGGCGATAACCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTACTTTCGCATTAGCAGCCCGCAgcgc    >  1:597542/3‑300 (MQ=255)                                                                                                                                                                                                                                                                        cccGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGTGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAgcgcat  >  2:410043/1‑298 (MQ=255)                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                        ACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCAGCCCGCAGCGCCT  >  NC_000913/4014958‑4015520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑