Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,015,220	A→C	N723T (AAC→ACC)	metE →	cobalamin‑independent homocysteine transmethylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,015,220	0	A	C	100.0%	198.8 / NA	56	N723T (AAC→ACC)	metE	cobalamin‑independent homocysteine transmethylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (27/29);  total (27/29)

TTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCA  >  NC_000913/4014925‑4015507                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                ttCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAcccc                                                                                                                                                                                                                                                                                              >  1:557044/1‑299 (MQ=255)     gtgtCAATGCCGCCGTGGCGAAAGAGGACACACAAAGCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCAGCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTTGGTCAccccatac                                                                                                                                                                                                                                                                                          <  2:379882/296‑5 (MQ=255)     gTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCgtac                                                                                                                                                                                                                                                                                          <  1:313151/299‑4 (MQ=255)     gTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTNGATTATCCAAATGAAATCGGTCCTGGCGTCTANGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCgtac                                                                                                                                                                                                                                                                                          <  2:487453/299‑4 (MQ=255)                                  acacaAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGaa                                                                                                                                                                                                                                                            >  1:589745/1‑300 (MQ=255)                                  acacaAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCATGAAAACGCGCGGCTGGCCAGaa                                                                                                                                                                                                                                                            >  1:589394/1‑300 (MQ=255)                                     caAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAAcc                                                                                                                                                                                                                                                         >  1:311918/1‑300 (MQ=255)                                                 cacaTGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACGTGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACt                                                                                                                                                                                                                                              >  1:613798/1‑299 (MQ=255)                                                 cacaTGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACt                                                                                                                                                                                                                                              >  1:630154/1‑299 (MQ=255)                                                    aTGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCg                                                                                                                                                                                                                                          <  1:507375/300‑1 (MQ=255)                                                         ttATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACAt                                                                                                                                                                                                                                     <  1:375220/300‑1 (MQ=255)                                                                   ttCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAggcg                                                                                                                                                                                                                           <  1:339008/300‑1 (MQ=255)                                                                                                gcTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGTAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATcc                                                                                                                                                                                              <  1:160676/300‑1 (MQ=255)                                                                                                gcTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATcc                                                                                                                                                                                              >  1:57065/1‑300 (MQ=255)                                                                                                    gACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAAc                                                                                                                                                                                          <  1:526148/300‑1 (MQ=255)                                                                                                     aCGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTaaaactcaaac                                                                                                                                                                                          >  2:102663/1‑292 (MQ=255)                                                                                                          gACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGggt                                                                                                                                                                                     >  1:55161/1‑299 (MQ=255)                                                                                                            cGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGggtggt                                                                                                                                                                                  >  2:455243/1‑300 (MQ=255)                                                                                                                 tcaCCATCGAAACCTCGCGTTCCGACATGGAGTTGCTTGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATac                                                                                                                                                                             <  2:431677/300‑1 (MQ=255)                                                                                                                   aCCATCGAAACCTCGCGTTCCTACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATacc                                                                                                                                                                            <  2:20184/299‑1 (MQ=255)                                                                                                                   aCCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGGGTCGTTTTAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCTAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATacc                                                                                                                                                                            <  2:20855/299‑1 (MQ=255)                                                                                                                     cATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTNAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCAGTACTGTGGCCTGAAAACGCGCGGCTGGCAAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATaccacc                                                                                                                                                                         >  2:466210/1‑300 (MQ=255)                                                                                                                     cATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCAGGCAGAGNGCCTGTGGGTCACCACGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATaccacc                                                                                                                                                                         >  2:194249/1‑300 (MQ=255)                                                                                                                          aaaCCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCAACCGGTc                                                                                                                                                                    >  2:145896/1‑300 (MQ=255)                                                                                                                          aaaCCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGAAATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCAGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGAGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAGTaccacacagtc                                                                                                                                                                    >  2:149175/1‑294 (MQ=255)                                                                                                                                 gcgTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTtctc                                                                                                                                                             <  1:493053/300‑1 (MQ=255)                                                                                                                                    ttCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGANCATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCAtt                                                                                                                                                          >  1:463575/1‑300 (MQ=255)                                                                                                                                         aCATGGAGTTGCTGTAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCACTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGc                                                                                                                                                     <  2:531210/300‑1 (MQ=255)                                                                                                                                         aCATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATGATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCAGTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACatc                                                                                                                                                     <  2:530959/300‑3 (MQ=255)                                                                                                                                                            gTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATAc                                                                                                                                   <  2:446622/299‑1 (MQ=255)                                                                                                                                                            gTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATAc                                                                                                                                   <  1:153233/299‑1 (MQ=255)                                                                                                                                                                 aaGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGTTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCtt                                                                                                                              <  1:302667/299‑1 (MQ=255)                                                                                                                                                                     gTTTGATTATCCAAGTGGGATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGGACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTTGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAGGATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAAcc                                                                                                                         <  2:146625/300‑1 (MQ=255)                                                                                                                                                                     gTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCTTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCNAGAAACCCGCGCGGCACTGGCGAACATGGTGCNGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAAcc                                                                                                                         <  2:482900/300‑1 (MQ=255)                                                                                                                                                                     gTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTTAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGGACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAAcc                                                                                                                         <  2:435706/300‑1 (MQ=255)                                                                                                                                                                     gTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAAcc                                                                                                                         <  1:488969/300‑1 (MQ=255)                                                                                                                                                                                   aTGAAATCGGTCTGGGCGTCTATTACATTCACTCGCCAAACGTACCAAGCGTGGAATGGATGGAAGCCTTGCTGAAGAAATCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCTGCACTGTCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGc                                                                                                           <  2:336077/300‑1 (MQ=255)                                                                                                                                                                                            gTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTANTACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTgccagcca                                                                                                  >  1:128504/1‑300 (MQ=255)                                                                                                                                                                                             tCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCAt                                                                                                 <  1:398528/300‑1 (MQ=255)                                                                                                                                                                                                 ggCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCAtcttc                                                                                             >  2:537391/1‑300 (MQ=255)                                                                                                                                                                                                    gTCTATGACATTCACTNGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTNAAACCATTCCAGCATACGNTGCCAGCCATCTTCTGc                                                                                          >  1:551811/1‑300 (MQ=255)                                                                                                                                                                                                      cTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCAGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCa                                                                                         >  2:110352/1‑299 (MQ=255)                                                                                                                                                                                                       tacgACATTCACTCGCCAAACGTACCGAGCTTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAGCTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCACTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAg                                                                                        <  2:73095/296‑1 (MQ=255)                                                                                                                                                                                                       tATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTTAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAg                                                                                        <  2:73432/299‑1 (MQ=255)                                                                                                                                                                                                        aTGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATACAAACCGGGTGGTAATACCAACCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCAAGCCATTTTCTGCAGAt                                                                                      >  2:46670/1‑300 (MQ=255)                                                                                                                                                                                                          gACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGc                                                                                    >  2:603165/1‑300 (MQ=255)                                                                                                                                                                                                                   tCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGAt                                                                            <  1:259893/299‑1 (MQ=255)                                                                                                                                                                                                                           cGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGc                                                                   >  2:599688/1‑300 (MQ=255)                                                                                                                                                                                                                           cGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTAAAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATCCGCTGCCAGCCATCTTCTGCAGATGTGGCCTGATAGCTAGGGc                                                                   >  2:295776/1‑300 (MQ=255)                                                                                                                                                                                                                                       ggAATGGATTGAAGCCTTGCTTAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTTGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGt                                                       <  2:328150/300‑1 (MQ=255)                                                                                                                                                                                                                                        gAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCAGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGGTAGCTCGGGCGATAATCAGCGtt                                                      >  2:37736/1‑300 (MQ=255)                                                                                                                                                                                                                                            ggATTGAATCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAat                                                  <  2:606230/300‑1 (MQ=255)                                                                                                                                                                                                                                            ggATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCATACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTG‑AGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAat                                                  <  1:209890/299‑1 (MQ=255)                                                                                                                                                                                                                                                                   gCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTacac                           <  1:186642/300‑1 (MQ=255)                                                                                                                                                                                                                                                                        aaaaCGCATTCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCAAGCCATCTTCTGCCGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGCATGCATGCCCGGCGTCCGGGTACACGATaa                      >  2:154534/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                ttCCGGCAGAGCGCCTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCNGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCt              >  1:468540/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             ccTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTagc   >  2:597964/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                             ccTGTGGGTCACCCCGTACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATAAGCGTTGCATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTagca  >  2:386184/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                TTCCGTATCAACGCCGCCGTGGCGAAAGATGACACACAAATCCACACTCACATGTGTTATTGCGAGTTCAACGACATCATGGATTCGATTGCGGCGCTGGACGCAGACGTCATCACCATCGAAACCTCGCGTTCCGACATGGAGTTGCTGGAGTCGTTTGAAGAGTTTGATTATCCAAATGAAATCGGTCCTGGCGTCTATGACATTCACTCGCCAAACGTACCGAGCGTGGAATGGATTGAAGCCTTGCTGAAGAAAGCGGCAAAACGCATTCCGGCAGAGCGCCTGTGGGTCAACCCGGACTGTGGCCTGAAAACGCGCGGCTGGCCAGAAACCCGCGCGGCACTGGCGAACATGGTGCAGGCGGCGCAGAACTTGCGTCGGGGGTAAAATCCAAACCGGGTGGTAATACCACCCGGTCTTTTCTCATTACAGCGACTTCTTCCCACCATACTGCTTAAACCATTCCAGCATACGCTGCCAGCCATCTTCTGCAGATGCGGCATGATAGCTCGGGCGATAATCAGCGTTGAATGCATGCCCGGCGTCCGGGTACACGATAATCTCTGCTTTCGCATTAGCA  >  NC_000913/4014925‑4015507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑