Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,925,200	T→C	K145R (AAG→AGG)	mnmG ←	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,925,200	0	T	C	100.0%	217.6 / NA	62	K145R (AAG→AGG)	mnmG	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/31);  total (31/31)

ACACGGCACCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGC  >  NC_000913/3924908‑3925494                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                       acacGGCACCTGCTGTGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTcc                                                                                                                                                                                                                                                                                                 <  2:410501/300‑1 (MQ=255) acacGGCACCTGCTGGNGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCAGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGATAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAANGGTGAGCACGACGGCTTTNGCACGGAACCTCCGTcc                                                                                                                                                                                                                                                                                                 >  1:543860/1‑300 (MQ=255)          ctgctgGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCAGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCAAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGt                                                                                                                                                                                                                                                                                        >  1:492541/1‑300 (MQ=255)              tgtggATGCTTGTACGCATTGCCCATAAACGAGAATACCTGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGTCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAAc                                                                                                                                                                                                                                                                                     <  2:182606/296‑1 (MQ=255)                  gATGCTGGGACGCATTGCCCATAAACTAGAGTACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCAc                                                                                                                                                                                                                                                                                <  2:525393/300‑1 (MQ=255)                  gATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATAGACATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCAc                                                                                                                                                                                                                                                                                <  1:570965/300‑1 (MQ=255)                  gATGCGGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCAc                                                                                                                                                                                                                                                                                <  2:525295/300‑1 (MQ=255)                   aTGCTGGGACGCATTGNCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTNGGCACGGAACCTCAGTCCNATTTGGGTAACAGCAcc                                                                                                                                                                                                                                                                               >  1:547843/1‑300 (MQ=255)                   aTGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCAcc                                                                                                                                                                                                                                                                               >  1:63388/1‑300 (MQ=255)                        gggACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGAACGATATGAATTTTACCGTCGAGGAACGTCCAAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCa                                                                                                                                                                                                                                                                          >  2:522192/1‑300 (MQ=255)                                  gCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCTAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGtt                                                                                                                                                                                                                                                                >  1:435881/1‑300 (MQ=255)                                  gCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGCAGCGGCAGCTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGtt                                                                                                                                                                                                                                                                >  1:249418/1‑300 (MQ=255)                                                     ggCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCAGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCa                                                                                                                                                                                                                                             >  1:413894/1‑300 (MQ=255)                                                      gCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCCATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGACTGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCACACGGTGAGCACGAAGGCTTTGGCACGGAACCTAAGTACCATTTGGGTAAAAGAAACGACCACACGATCGCTTTCGACAATACGATCTTCaa                                                                                                                                                                                                                                            >  2:133359/1‑300 (MQ=255)                                                        aTTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCAATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAAc                                                                                                                                                                                                                                           <  2:389378/299‑1 (MQ=255)                                                           gggTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGCAGCTGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGcc                                                                                                                                                                                                                                       >  1:515296/1‑300 (MQ=255)                                                           gggTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCNTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGcc                                                                                                                                                                                                                                       >  1:469247/1‑300 (MQ=255)                                                                 tCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCAGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCAACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCAAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCctgct                                                                                                                                                                                                                                   >  2:217935/1‑298 (MQ=255)                                                                    ccATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGaa                                                                                                                                                                                                                               >  2:395222/1‑299 (MQ=255)                                                                         cTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCACCGGTGAGCACGAAGGCTTTGGCACGGAACCTCAGTCCAATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCctgct                                                                                                                                                                                                                                   >  2:227532/1‑290 (MQ=255)                                                                                 ccAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCAAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTc                                                                                                                                                                                                                  >  2:96868/1‑299 (MQ=255)                                                                                             aGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCa                                                                                                                                                                                                     >  1:94170/1‑300 (MQ=255)                                                                                                                     gcgGTGGTGTCCCGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACgg                                                                                                                                                                              <  1:395222/299‑1 (MQ=255)                                                                                                                            tttCCCGGTTTGCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGcac                                                                                                                                                                      <  2:149533/298‑1 (MQ=255)                                                                                                                                  ggTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATc                                                                                                                                                                <  1:301835/300‑1 (MQ=255)                                                                                                                                           acgacCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGNCCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGc                                                                                                                                                       <  2:507014/300‑1 (MQ=255)                                                                                                                                             gacCCACGCGCAGCGGTAGTTCATGCAAACGGCGAGAAAGCTGAATGGACGGTGGATCATCAGCGCGGCCACCGCTGTAATTATCCGGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACTGAACCTCAGTCCCATTTGGGTAACAGCACCTACCACGCGATCGTTTGCGACAAGAAGATCTTCAACCGCTTGTTGTAAGATCATTAAGTTTGTTTGGTTCTCCAGCGCCGTACGTACCGCCTGACTGTAGAGCACACGATCCGCCTGatct                                                                                                                                                      <  2:215235/299‑4 (MQ=255)                                                                                                                                                 aaCGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGt                                                                                                                                                 <  1:385447/300‑1 (MQ=255)                                                                                                                                                           ggCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAAcc                                                                                                                                       <  1:328559/300‑1 (MQ=255)                                                                                                                                                                       aaaCGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGATCGATATGAATTTTACCGTCGATGAACGTCCCAACGGTGAGCACGACTGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATTTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCt                                                                                                                                                      <  2:215306/273‑1 (MQ=255)                                                                                                                                                                         aCGGCGAGAAAGCGGAANGGACGGCGGATCACCAGCNCGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGANCCGCCGGTCCTttgct                                                                                                                         <  1:540898/300‑1 (MQ=255)                                                                                                                                                                                            gACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTTGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGTTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAAc                                                                                                      <  2:312617/300‑1 (MQ=255)                                                                                                                                                                                            gACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGGCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAAATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAAc                                                                                                      <  2:312999/300‑1 (MQ=255)                                                                                                                                                                                             aCGGCGGATCACCAGCACTGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTGAGTATCCTAAAc                                                                                                      <  2:312922/299‑1 (MQ=255)                                                                                                                                                                                                      cACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGNAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATAccc                                                                                             >  2:199022/1‑299 (MQ=255)                                                                                                                                                                                                      cACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATAccc                                                                                             >  1:250367/1‑299 (MQ=255)                                                                                                                                                                                                                    ccGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCATCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCt                                                                              >  1:408309/1‑300 (MQ=255)                                                                                                                                                                                                                    ccGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCt                                                                              >  1:96255/1‑300 (MQ=255)                                                                                                                                                                                                                                   aGACCGATATGAAGTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACTGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGAc                                                                 <  2:354787/298‑1 (MQ=255)                                                                                                                                                                                                                                                ttttACCGTCTAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCa                                                  <  2:262952/300‑1 (MQ=255)                                                                                                                                                                                                                                                 tttACCGTCTATGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGTTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCNGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCACTTTCGCCATCAGACCGCCGAGTGCATCCAc                                                 <  2:212558/300‑1 (MQ=255)                                                                                                                                                                                                                                                       gTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCttt                                           >  1:220015/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 gTCCCAACGGTGAGCACGACGGCTTTTGCACTGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGGTGGTTCTCCAGCGCCGTACGTACCGCCTTACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATg                                  <  2:256091/299‑1 (MQ=255)                                                                                                                                                                                                                                                                  tCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCANCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTCCCAGATGTc                                >  2:464164/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  tCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGt                                                                                                                                                 <  1:400200/187‑1 (MQ=255)                                                                                                                                                                                                                                                                  tCCCAACGGTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGt                                                                                                                                                 >  2:400200/1‑187 (MQ=255)                                                                                                                                                                                                                                                                         ggTGAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCCTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTccc                         <  2:96255/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            gAGCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCAGTACGTACCGCCTGACGGTAGAGCACACGATCCGCATGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTCCCAGGTGTCCCTTCCCaa                       >  2:258864/1‑299 (MQ=255)                                                                                                                                                                                                                                                                              gCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTTGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATAc                    <  2:300571/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              gCACGACGGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTTACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAAATGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATAc                    <  1:567307/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              gCACGACGGCGTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATACGATCTTCAACCGCCTGCTTGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATAc                    <  2:350194/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  gacgGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGANCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATAccgc                 <  2:477585/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                  gacgGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATAccgcc                >  1:43315/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  gacgGCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATAccgc                 <  1:626391/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                      gCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGGTCTTAAACCGCATGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTCGCTTGCGTTTAGTATCCTAAACTGGATACCCGCATGATCGATCGCTTTCGCCATCAGACCGCCGAATGCACCTACTTCTTTTAACAGGTGTCCATTCACaacacagacgat             >  2:347077/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                      gCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCCACCGCCTGCTGGAAGATTATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCATGACGGTAGAGCACACGATCCGAATGAGCTCGGGTAGCGCGAACCGCAGGTACTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTACCTTCCCAATACCGACGATc            >  2:347075/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      gCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATc            >  1:108994/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      gCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCATTCCCAATACCGCCGATc            >  1:392497/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      gCTTTGGCACGGAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGAAAATAAGATCTTCAACCGCCTGCTGGAAGATTATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCAATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTACCAATACCGCAGATc            >  2:346557/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      gCTTTGGCACGGAACCNCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTCTCTACACTAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACNTCTTTTACCAGATGTCCCNTCCCCATACCGCCGAtt            >  1:543592/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                ggAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGc  <  2:547843/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 gAACCTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTACAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGc  <  2:63388/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                       ACACGGCACCTGCTGGGGATGCTGGGACGCATTGCCCATAAACGAGAATACCGGCATTGGGTTATCGCCATGCTGTTGCGCCAGTACGCTAAAGTCGATGGTTCGAGCATCAATACGCGGTGGTGTCCCGGTTTTCAGACGACCAACGCGCAGCGGCAGTTCACGCAAACGGCGAGAAAGCGGAATGGACGGCGGATCACCAGCACGGCCACCGCTGTAATTATCCAGACCGATATGAATTTTACCGTCGAGGAACGTCCCAACGGTGAGCACGACGGCTTTGGCACGGAACTTCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTCGACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATACCGCCGATCGCCGGGTTGC  >  NC_000913/3924908‑3925494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑