Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,878,536	G→A	Y528Y (TAC→TAT)	gyrB ←	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,878,536	0	G	A	100.0%	186.3 / NA	52	Y528Y (TAC→TAT)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/33);  total (19/33)

ACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCA  >  NC_000913/3878243‑3878827                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                    acgttCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAACTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCTTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTTGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACg                                                                                                                                                                                                                                                                                               <  2:91524/296‑1 (MQ=255) aCGGTCTGCTCATCATAAAGGTCAGCTTCCGTCAACTTCGGCTGATAGATAAGCTCTTTCAGCATTTCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCTTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACg                                                                                                                                                                                                                                                                                               <  2:142554/300‑1 (MQ=255) aCGGTCTGCTCATCAGAAAGTTCAGCTTCCTTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACg                                                                                                                                                                                                                                                                                               <  2:33764/300‑1 (MQ=255) aCGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAANGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACg                                                                                                                                                                                                                                                                                               <  1:127311/300‑1 (MQ=255) aCGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACg                                                                                                                                                                                                                                                                                               <  1:650091/300‑1 (MQ=255)   ggTCTGCTCGTCAGAAAGTTCAGTTTCCTTCAACGTCGGCTGATAGATAAGTTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCTTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGAGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACg                                                                                                                                                                                                                                                                                               <  2:240048/298‑1 (MQ=255)     tCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCGGATAGATAAGCTCTTTCAGCATTGCTTGCGGATAACGACGCTCCATACGAGTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCTGCGGCTAAGCGATATAGACg                                                                                                                                                                                                                                                                                               <  2:142549/296‑1 (MQ=255)           catcaGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGtt                                                                                                                                                                                                                                                                                     >  1:594022/1‑300 (MQ=255)                 aaaGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGa                                                                                                                                                                                                                                                                               <  1:383080/300‑1 (MQ=255)                                        ggCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATagaa                                                                                                                                                                                                                                                         >  2:553620/1‑299 (MQ=255)                                                    aGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCNGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAAcagc                                                                                                                                                                                                                                            <  1:137359/300‑1 (MQ=255)                                                           tCAGCATTGCTTTATGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCNTCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTAc                                                                                                                                                                                                                                     <  2:465615/300‑1 (MQ=255)                                                              gCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCTGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGaa                                                                                                                                                                                                                                  <  1:155006/300‑1 (MQ=255)                                                              gCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGaa                                                                                                                                                                                                                                  <  2:454057/300‑1 (MQ=255)                                                              gCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGaa                                                                                                                                                                                                                                  <  1:155027/300‑1 (MQ=255)                                                              gCATTGCTTTCGGATAACGACGCTCCATACGATGGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCACCAGCCAATGCCGTTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGaa                                                                                                                                                                                                                                  <  2:32014/300‑1 (MQ=255)                                                              gCATTGCTTTCGAATAACGACGCTCCATACGAGTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGaa                                                                                                                                                                                                                                  <  2:623032/300‑1 (MQ=255)                                                                     tttCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGa                                                                                                                                                                                                                           <  1:37019/300‑1 (MQ=255)                                                                                                 aTCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCatgatg                                                                                                                                                                                               <  2:532405/300‑1 (MQ=255)                                                                                                          tGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCtg                                                                                                                                                                                      <  1:153465/300‑1 (MQ=255)                                                                                                                gcgTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATaa                                                                                                                                                                                >  1:251826/1‑300 (MQ=255)                                                                                                                gcgTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTAATGATGATGATGCTGTGATaa                                                                                                                                                                                >  1:453361/1‑300 (MQ=255)                                                                                                                  gTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAAc                                                                                                                                                                               >  2:643208/1‑299 (MQ=255)                                                                                                                     gTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCa                                                                                                                                                                            >  1:245209/1‑299 (MQ=255)                                                                                                                           aGATACCAGTTTCTCTAACTCTTCGCCAGCCAATGCCGGTGCACTTGCGTTGGTGTGCAGCGTTGCGCCGTCCATCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCTGCTGAGCGATATAGACg                                                                                                                                                                                                                                                                                               <  2:91060/178‑1 (MQ=255)                                                                                                                                  aGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGtt                                                                                                                                                               <  2:644299/299‑1 (MQ=255)                                                                                                                                     ttCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTAc                                                                                                                                                           <  1:389612/300‑1 (MQ=255)                                                                                                                                          tAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGTTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTc                                                                                                                                                         <  2:91990/297‑1 (MQ=255)                                                                                                                                          tAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTc                                                                                                                                                      <  1:553620/300‑1 (MQ=255)                                                                                                                                              gCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACg                                                                                                                                                   <  1:201904/299‑1 (MQ=255)                                                                                                                                                ttCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGAc                                                                                                                                                 <  2:594022/299‑1 (MQ=255)                                                                                                                                                      agccaATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGAccaacacc                                                                                                                                          >  1:251766/1‑294 (MQ=255)                                                                                                                                                      agccaATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGAACGATAcc                                                                                                                                          >  1:251965/1‑300 (MQ=255)                                                                                                                                                             gccggtatacttgCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGGGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCa                                                                                                                                   <  2:22703/288‑1 (MQ=255)                                                                                                                                                                       tGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGa                                                                                                                         <  1:591661/300‑1 (MQ=255)                                                                                                                                                                         gCGTTGGTGTGCAGCGTTGCGCCGTCAAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTACGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATa                                                                                                                       >  2:632318/1‑300 (MQ=255)                                                                                                                                                                              ggTGTGCAGCGTGGCGCCGTCCAGCTCGATAGAGATTGGGTACTGATCCATCGCTGCGTCGTCTTGAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGGGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAAAGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGt                                                                                                                  <  1:261369/300‑1 (MQ=255)                                                                                                                                                                                      gCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACtt                                                                                                          >  1:95967/1‑300 (MQ=255)                                                                                                                                                                                                gTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaaga                                                                                                >  1:571578/1‑300 (MQ=255)                                                                                                                                                                                                               gaTCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGaa                                                                                 >  2:454436/1‑300 (MQ=255)                                                                                                                                                                                                               gaTCTGATCCTGATTCATCGCTTCGTCGTCTTTACTGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGACGGCTGAGCGATATAGACGTGACCGCGTTCAACGGTTTCCGGCATCTGACGATAGAAGAAGGTCAACAGTAGTGTACGAACGTGCGAGCCATCGACGCCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGAACGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCAATTCCTGCGAAGATAGCATCTCATCGa                                                                                  >  2:454494/1‑299 (MQ=255)                                                                                                                                                                                                                     gTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGAACGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAgcgcgc                                                                           >  2:392146/1‑300 (MQ=255)                                                                                                                                                                                                                          gATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTtct                                                                      >  1:322864/1‑300 (MQ=255)                                                                                                                                                                                                                                       gtcgttTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGNTCAACGATTTCCGGCATCTGACGAGAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTTTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAt                                                         <  1:128257/300‑1 (MQ=255)                                                                                                                                                                                                                                                    tACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCNGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGc                                            <  1:471762/300‑1 (MQ=255)                                                                                                                                                                                                                                                    tACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGc                                            <  1:354199/300‑1 (MQ=255)                                                                                                                                                                                                                                                      cTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGAAGTTGAGGATTTTACCCTTCAGc                                            >  2:603025/1‑298 (MQ=255)                                                                                                                                                                                                                                                        gTTCCTGCTTGCCTTTCTTAACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCa                                        >  1:28337/1‑300 (MQ=255)                                                                                                                                                                                                                                                              gCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCg                                  <  2:542783/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            aCTTTGTACAGCGGAGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGctg                    <  1:433420/300‑3 (MQ=255)                                                                                                                                                                                                                                                                               ttGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCCGCGGCAGAATCGCCTGGTTCTTGCGGTTa                 >  2:40111/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               gAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCa  >  1:233851/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                    ACGGTCTGCTCATCAGAAAGGTCAGCTTCCGTCAACGTCGGCTGATAGATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCA  >  NC_000913/3878243‑3878827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑