Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,725,176	T→G	E48A (GAG→GCG)	glyQ ←	glycine‑‑tRNA ligase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,725,176	0	T	G	100.0%	312.1 / NA	87	E48A (GAG→GCG)	glyQ	glycine‑‑tRNA ligase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (38/49);  total (38/49)

ACTGCGTCACTTCCATGCCGTTCAGCCACACTTCCCAGCCCAGTCCCCAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACA  >  NC_000913/3724886‑3725460                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                   aCTGCGTCACTTCCATATCGTTCAGCCACACTTCCCAGCCCAGTCCCCAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACTAAACAGATGTCGTGAATAGTCGGTTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCGGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGAc                                                                                                                                                                                                                                                                                           <  2:91112/300‑1 (MQ=255)         aCTTCCATGCCGTTCAGCCACACTTCCCAGCCCAGTCCCCAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCAtt                                                                                                                                                                                                                                                                                   <  1:267125/300‑1 (MQ=255)          cTTCCATGCCGTTCAGCCACACTTCCCAGCCCAGTCCCCAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCAtt                                                                                                                                                                                                                                                                                   >  1:594234/1‑299 (MQ=255)          cTTCCATGCCGTTCAGCCACACTTCCCAGCCCAGTCCCCAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCAtt                                                                                                                                                                                                                                                                                   >  2:77850/1‑299 (MQ=255)          cTTCCATGCCGTTCAGCCACACTTCCCAGCCCAGTCCCCAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTAGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGACTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAAGTCAtt                                                                                                                                                                                                                                                                                   >  2:39968/1‑299 (MQ=255)                    gTTCAGCCACACTTCCCAGCCCAGTCCCCAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAgg                                                                                                                                                                                                                                                                         <  1:446681/299‑1 (MQ=255)                             tccttacaagcacagTCCCCAGGCACCCAGGGTCGGGTTTTCCCAGTTCTTTTCCCCAAAAAGGCTGTCTTGAATAGCCGGGTCCCTGCCCAGCT‑‑CTTTCAAAGAACCGAGTTACAGTTCCTGAGTGTTGTCTGGCGATGGCTTAATGACCACCTGGACCTGATAGCAGTGTTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTTGGGCGACAAGAAGATTGAACATAATCAGCAGCCGTCGGTTTTGGCCCC‑AGCGAGCACAGACAGGTCCTTGGGTGGGAGGTTCCCGCGcc                                                                                                                                                                                                                                                               >  2:90914/13‑300 (MQ=255)                               cTTCCCAGCCCAGTCCCCAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGNACCGAGGTACAGCTCCTGAATATTGTCCGGCGNTGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTCCCCGCGCCGa                                                                                                                                                                                                                                                             >  2:494979/1‑300 (MQ=255)                                 tCCCAGCCCAGTCCCCAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACt                                                                                                                                                                                                                                                           >  1:269082/1‑300 (MQ=255)                                          agTCCCCAGGCACCCAGCTTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGTTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTc                                                                                                                                                                                                                                                   <  2:157111/299‑1 (MQ=255)                                           gTCCCCAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCCTGTCCa                                                                                                                                                                                                                                                 >  2:94687/1‑300 (MQ=255)                                              cccAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAAt                                                                                                                                                                                                                                               >  1:396181/1‑299 (MQ=255)                                               ccAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCAGCGCCGACTTCCATGTCCAAt                                                                                                                                                                                                                                               >  1:269135/1‑298 (MQ=255)                                                 atgCACCCAGCGTCGGGTTTTCCCAGTTATCTTCTACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGTTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATgg                                                                                                                                                                                                                                             <  1:266740/296‑1 (MQ=255)                                                 atgCACCCAGCGGCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAACT‑‑CTTTCAGAGAACCGAAGTACAGCTCCTGAACATTGTCCGGCGATGGCTTAATTACCACCTGGAACTGATATTAGTGCTGTAAACGGGTTGGGTTTTCGCCGTAGCGACCATCGGTCGGTCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCACAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATgg                                                                                                                                                                                                                                             <  1:267380/296‑1 (MQ=255)                                                 aGGCACCCAGCTTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATgg                                                                                                                                                                                                                                             <  1:267004/298‑1 (MQ=255)                                                            gTCGGTTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCTAGGTACAGCTCCTGAATATTGTCCTGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGTTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACaa                                                                                                                                                                                                                                     <  2:1029/295‑1 (MQ=255)                                                               gggTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGNGAACCGAGGTACAGCTCCTGAATATTGTCCGTCGATGGCTTAATGACCACCGGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCTGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTc                                                                                                                                                                                                                                                   <  2:500946/278‑1 (MQ=255)                                                               gggTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGNACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑Ag                                                                                                                                                                                                                           <  1:125745/300‑1 (MQ=255)                                                                  ttttCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGccc                                                                                                                                                                                                                        <  2:647745/300‑1 (MQ=255)                                                                         aGTTATCTTCCACTAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGTTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCg                                                                                                                                                                                                                 <  2:231229/298‑1 (MQ=255)                                                                         aGTTATCTTCCACGAAACGGATGTCGTGAATATTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCTAGGTACAGCTCCTGAATATTGTCCGGCGATTGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAACAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCg                                                                                                                                                                                                                 <  2:297086/298‑1 (MQ=255)                                                                         aGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTTAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCg                                                                                                                                                                                                                 <  2:231258/298‑1 (MQ=255)                                                                         aGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCg                                                                                                                                                                                                                 <  1:607192/298‑1 (MQ=255)                                                                                    aCGAAATGGATGTAGTGAATAGTCGGGTTCATGCCCGGCT‑‑CTTTTAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATATTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑cc                                                                                                                                                                                                  <  2:219536/300‑1 (MQ=255)                                                                                      gAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTGCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAAGGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGATAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTg                                                                                                                                                                                                <  2:136617/300‑1 (MQ=255)                                                                                        aaCGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTNATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCNGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTa                                                                                                                                                                                              >  2:497576/1‑300 (MQ=255)                                                                                                 cGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCAATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCNTTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGa                                                                                                                                                                                     >  2:515700/1‑300 (MQ=255)                                                                                                        attCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGATGTACAGCTCCTGAATATTGTCCGTCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGcc                                                                                                                                                                              <  2:547535/298‑1 (MQ=255)                                                                                                        aGTCGGTTCCATGCCCAGCT‑‑CTTTCAGAGAACCTAGGTACAGCTCCTTAATATTGTCCGTCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGTTTGGGGTTTTCGCCGTAGCGACCATCGNTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGcc                                                                                                                                                                              <  2:206322/300‑1 (MQ=255)                                                                                                         gTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGccc                                                                                                                                                                             <  1:357356/300‑1 (MQ=255)                                                                                                         gTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTACCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGccc                                                                                                                                                                             <  1:604591/300‑1 (MQ=255)                                                                                                          tCGGGGCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCTGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGNCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGATAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAAGTCAGGATCAAGccc                                                                                                                                                                             <  2:202861/299‑1 (MQ=255)                                                                                                             ggTCCATGCCCAGCTCNCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCGAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCCCAGCCCGNTGGCGAGCCCAGTAATTGNCTGTAAGGTCAgcggat                                                                                                                                                                                  >  1:535755/1‑295 (MQ=255)                                                                                                               tCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGaa                                                                                                                                                                        >  2:554842/1‑299 (MQ=255)                                                                                                                    gCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCt                                                                                                                                                                  >  2:377555/1‑300 (MQ=255)                                                                                                                    gCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGGAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTTAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGaa                                                                                                                                                                        <  1:365506/294‑1 (MQ=255)                                                                                                                    gCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGAACACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTAAGGATCAAGCCCTGGAAGGtact                                                                                                                                                                  >  2:51467/1‑297 (MQ=255)                                                                                                                                                     tCCTGAAGATTGTCCTGCGATGGCTTAATGACCACCTGGAACTGATATTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGTTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGc                                                                                                                                    <  2:65478/299‑1 (MQ=255)                                                                                                                                                      ccTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCCTTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCACACTTTTGCATATTATTTCGTGCt                                                                                                                                   >  2:279000/1‑299 (MQ=255)                                                                                                                                                            tatTGTCCTGCGATGGCNTAATGACCACCTGGAACTNATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTNTTTCGTGCTGGATACg                                                                                                                            <  1:552974/300‑1 (MQ=255)                                                                                                                                                            tatTGTCCGGCGATGGCNTAATGACCACCTGGAACTNATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTNTTTCGTGCTGGATACg                                                                                                                            <  1:552975/300‑1 (MQ=255)                                                                                                                                                              ttGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGTTCGGTCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATActtg                                                                                                                          <  2:80167/300‑4 (MQ=255)                                                                                                                                                                    ggCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGAtt                                                                                                                    >  1:559023/1‑300 (MQ=255)                                                                                                                                                                         tGGCTTAACGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGTTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAaag                                                                                                               <  2:150449/300‑1 (MQ=255)                                                                                                                                                                          ggCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTaaa                                                                                                                >  1:512567/1‑298 (MQ=255)                                                                                                                                                                          ggCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTaaa                                                                                                                >  2:603760/1‑298 (MQ=255)                                                                                                                                                                          ggCTTAATGAACACCTGGAAATGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCagat                                                                                                                                              >  2:248681/1‑265 (MQ=255)                                                                                                                                                                           gCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCACTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGCTTTAaagga                                                                                                             >  2:328321/1‑300 (MQ=255)                                                                                                                                                                                      accTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCTAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGta                                                                                                  >  1:623538/1‑300 (MQ=255)                                                                                                                                                                                      accTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGta                                                                                                  >  1:533276/1‑300 (MQ=255)                                                                                                                                                                                      accTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGt                                                                                                   <  1:437913/299‑1 (MQ=255)                                                                                                                                                                                         tGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATAc                                                                                               <  2:580078/300‑1 (MQ=255)                                                                                                                                                                                          ggAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATNAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATAcc                                                                                              <  1:461675/300‑1 (MQ=255)                                                                                                                                                                                           gAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCCATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATAccc                                                                                             >  2:621323/1‑300 (MQ=255)                                                                                                                                                                                           gAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATAccc                                                                                             >  2:621329/1‑300 (MQ=255)                                                                                                                                                                                           gAACTGATAGGAGTGCTGTAAACAGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCGGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATAccc                                                                                             <  2:180586/300‑1 (MQ=255)                                                                                                                                                                                             aCTGATAGTAGTGCTGTAAACGGTTTGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATGAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGGTGAACAATGGTGC‑‑AGCCC‑‑TTGCGAGCCTAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGc                                                                                           <  2:248398/300‑1 (MQ=255)                                                                                                                                                                                                  tagtagTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCtggat                                                                                      >  1:100420/1‑300 (MQ=255)                                                                                                                                                                                                       gtGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAg                                                                                 <  2:412472/300‑1 (MQ=255)                                                                                                                                                                                                        tGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAg                                                                                 <  1:191231/299‑1 (MQ=255)                                                                                                                                                                                                         gCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CATGTAAGGTCAGGATCAAGCACTGGAAGGTACTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATCTAAAGGAAAGGGCCAGTATACCCGCTGGATGGAAGat                                                                               >  2:279018/1‑300 (MQ=255)                                                                                                                                                                                                            gTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGAtata                                                                            >  2:154235/1‑300 (MQ=255)                                                                                                                                                                                                             tAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATAc                                                                           <  1:383709/300‑1 (MQ=255)                                                                                                                                                                                                              aaaCGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACa                                                                          >  1:24871/1‑300 (MQ=255)                                                                                                                                                                                                                 cGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTa                                                                       >  2:110192/1‑300 (MQ=255)                                                                                                                                                                                                                       gggTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAAc                                                                 <  1:643709/300‑1 (MQ=255)                                                                                                                                                                                                                         gTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGaaa                                                                  >  2:243024/1‑297 (MQ=255)                                                                                                                                                                                                                                  gTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGc                                                       <  1:652152/299‑1 (MQ=255)                                                                                                                                                                                                                                       gACCATCGGTCGGTCGACGAGAAGGCTGAACATAAGCATCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TTGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGc                                                  <  2:289761/299‑1 (MQ=255)                                                                                                                                                                                                                                       gACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTTAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAGTGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGc                                                  <  2:289796/299‑1 (MQ=255)                                                                                                                                                                                                                                        aCCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCtt                                                >  2:187682/1‑300 (MQ=255)                                                                                                                                                                                                                                        aCCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGAGCtt                                                >  2:187006/1‑300 (MQ=255)                                                                                                                                                                                                                                         ccATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTa                                               >  2:521101/1‑300 (MQ=255)                                                                                                                                                                                                                                           aTCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAAc                                             <  1:590058/300‑1 (MQ=255)                                                                                                                                                                                                                                            tcggtcggGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGTTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACg                                            <  2:550212/300‑1 (MQ=255)                                                                                                                                                                                                                                            tcggtcggGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACg                                            <  2:550103/300‑1 (MQ=255)                                                                                                                                                                                                                                                   ggCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGaa                                     <  2:594234/300‑1 (MQ=255)                                                                                                                                                                                                                                                    gCGACGGGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGTGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGaaa                                    <  2:230074/300‑1 (MQ=255)                                                                                                                                                                                                                                                    gCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTGCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGaaa                                    <  2:230063/300‑1 (MQ=255)                                                                                                                                                                                                                                                      gacgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAg                                   <  1:343202/299‑1 (MQ=255)                                                                                                                                                                                                                                                             aGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCaaaa                           >  1:383101/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   aaCATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGATAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGc                     <  1:595325/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                gccATCGGTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGCTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCaaa        <  2:396181/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                       gTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACa  >  2:392080/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                       gTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACa  >  1:97829/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                       gTTCTGGCCCC‑AGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCAC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAAAGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCaaaaaagca  >  2:91228/1‑294 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                   ACTGCGTCACTTCCATGCCGTTCAGCCACACTTCCCAGCCCAGTCCCCAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCT‑‑CTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCC‑AGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGC‑‑AGCCC‑‑TGGCGAGCCCAGTAAT‑‑CCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATGGAAAGATGCAAAAAATGGCCATCCGCGTCAAAAGAACA  >  NC_000913/3724886‑3725460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑