Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,579,943 G→A G103D (GGC→GAC)  tktB → transketolase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,579,9430GA94.6% 173.5 / ‑7.0 56G103D (GGC→GAC) tktBtransketolase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (32/21);  minor base C (3/0);  total (35/21)
Fisher's exact test for biased strand distribution p-value = 2.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGC  >  NC_000913/2579648‑2580237
                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                      
gACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGGTGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACACGTCGATGCTGCTCTAAAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCAGGAGATTGACtat                                                                                                                                                                                                                                                                                                    >  2:354522/1‑300 (MQ=255)
gACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCAAGGCCACCCGGAGATTGACtat                                                                                                                                                                                                                                                                                                    >  2:354317/1‑300 (MQ=255)
                       aCTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAaccaccac                                                                                                                                                                                                                                                                             <  2:573497/300‑1 (MQ=255)
                       aCTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAaccaccac                                                                                                                                                                                                                                                                             <  1:516308/300‑1 (MQ=255)
                        cTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAaccaccacc                                                                                                                                                                                                                                                                            >  1:43183/1‑300 (MQ=255)
                        cTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACACGGAGATTGACTATACGCCAGGCGTTGAAaccaccacc                                                                                                                                                                                                                                                                            >  1:310056/1‑300 (MQ=255)
                          cAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCTCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAaccaccacc                                                                                                                                                                                                                                                                            >  1:432073/1‑298 (MQ=255)
                          cAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATAAGCCAGGCGTTGAAaccaccacc                                                                                                                                                                                                                                                                            >  1:432066/1‑298 (MQ=255)
                            gTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCTGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGcc                                                                                                                                                                                                                                                                        >  1:412607/1‑300 (MQ=255)
                            gTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCGCCCGGAGGTTGACTATACGCCAGGCGGTGAAACCACCACGGGcc                                                                                                                                                                                                                                                                        >  1:225736/1‑300 (MQ=255)
                            gTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGcc                                                                                                                                                                                                                                                                        >  1:517009/1‑300 (MQ=255)
                            gTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAATTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGcc                                                                                                                                                                                                                                                                        <  2:155353/300‑1 (MQ=255)
                                       gTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTTAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACaa                                                                                                                                                                                                                                                             >  1:554596/1‑300 (MQ=255)
                                           aaaaaGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGt                                                                                                                                                                                                                                                          <  1:308140/299‑1 (MQ=255)
                                                   aaCTCTGTTCATCCCTGCGCGCCGATGGGCATGGCTGATATTGCCTAAGTGCTTTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTTAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGc                                                                                                                                                                                                                                                     <  2:94648/296‑1 (MQ=255)
                                                                           aTGGGCATTGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGTTCACGCTTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGTAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCagag                                                                                                                                                                                                                         <  2:61409/300‑1 (MQ=255)
                                                                               gCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTa                                                                                                                                                                                                                     >  1:71227/1‑300 (MQ=255)
                                                                                  tggatGATATTGCCGAAGTGCTGTGGCACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTACAACAGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGCAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCAGTAGGGCTGGTGATAGTAGAGCGTacac                                                                                                                                                                                                                  >  2:314590/5‑300 (MQ=255)
                                                                                  tGGCTGATATTGCCGAGGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAAACTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCAACCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTacac                                                                                                                                                                                                                  >  2:3756/1‑300 (MQ=255)
                                                                                  tGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACATGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCAGGAGATTGCCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTacac                                                                                                                                                                                                                  >  2:313729/1‑300 (MQ=255)
                                                                                  tGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTAACGACCCAAACTGGTATGATCGCGACCGCTTTATTCTTTACAACGGTCAAGCGTCGATGCTGCTCTACAGTTTGCTACATCTGAACGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGACAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCAACACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCCGAGCGTa                                                                                                                                                                                                                     >  2:314942/1‑297 (MQ=255)
                                                                                   ggCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACt                                                                                                                                                                                                                 >  1:420607/1‑300 (MQ=255)
                                                                                                    tGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAAcc                                                                                                                                                                                                <  2:541325/300‑1 (MQ=255)
                                                                                                     gCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCAACACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAAcca                                                                                                                                                                                               >  2:12/1‑300 (MQ=255)
                                                                                                                  tttCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGAGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCACAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATgag                                                                                                                                                                                  <  1:349475/300‑1 (MQ=255)
                                                                                                                    tCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGAt                                                                                                                                                                                <  1:647933/300‑1 (MQ=255)
                                                                                                                          aCATAACCCTACCGACCCCACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCAACCGGAGATTGCCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGCCAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGAtcgtcta                                                                                                                                                                          >  2:5147/1‑298 (MQ=255)
                                                                                                                                    aCCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCAcc                                                                                                                                                                <  1:572000/300‑1 (MQ=255)
                                                                                                                                            aaCCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGtt                                                                                                                                                        >  1:312063/1‑300 (MQ=255)
                                                                                                                                             aCCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGttt                                                                                                                                                       >  1:630349/1‑300 (MQ=255)
                                                                                                                                                  gTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGATAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTAt                                                                                                                                                     >  2:118899/1‑297 (MQ=255)
                                                                                                                                                  gTATGATCGCGACCGCTTTACTCTTTCCAACGGTCACGCGTCGACGCTGCTCTACAGTTCGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCAAGGCCACCCGGAGATTGACTATACGCCAGACGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCAATAGCAGAGCGTACACTGGCGACGCAGTTTAACCAGCCAGACCATGAGATCATCGATCACTTAACCTATGTGtttct                                                                                                                                                     >  2:119143/1‑295 (MQ=255)
                                                                                                                                                          gcgACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGc                                                                                                                                           <  2:571079/299‑1 (MQ=255)
                                                                                                                                                                               aCGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTccc                                                                                                                     <  1:93501/300‑1 (MQ=255)
                                                                                                                                                                                cGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCa                                                                                                                    >  1:348902/1‑300 (MQ=255)
                                                                                                                                                                                        cgTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTAACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCt                                                                                                            >  1:316584/1‑300 (MQ=255)
                                                                                                                                                                                        cgTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGAACTGCCGCTGGAAGAACTGAAAAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTCTACGCCAGGCGTTGAAACCACCACCGGCCCGATTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTACCACGAAGTCt                                                                                                            >  2:228751/1‑300 (MQ=255)
                                                                                                                                                                                             aTGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGGTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCg                                                                                                       <  2:324304/300‑1 (MQ=255)
                                                                                                                                                                                                ctgctCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCGGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTcact                                                                                                     >  2:87500/1‑296 (MQ=255)
                                                                                                                                                                                                ctgctCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCt                                                                                                     >  1:375103/1‑299 (MQ=255)
                                                                                                                                                                                                  gctCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTggc                                                                                                  >  1:31001/1‑300 (MQ=255)
                                                                                                                                                                                                  gatctACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGCCTATACGCCAGGTGTTGAAACCAACACCGACCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGTGTACACTGGCGGCGCAGTTTAACCAGCCAGACTATGAGATCGTCGATCATTTCACCTATGGGTTTATGGGCGACGGCTGCCTAATAGAAGGTATTTAACACGAAGTCTGTTCGCTggc                                                                                                  >  2:517763/3‑300 (MQ=255)
                                                                                                                                                                                                     ctACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCAACTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTggca                                                                                                 >  1:161871/1‑298 (MQ=255)
                                                                                                                                                                                                         aGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCNGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCAc                                                                                            <  1:461410/299‑1 (MQ=255)
                                                                                                                                                                                                             tGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCt                                                                                         >  2:225646/1‑298 (MQ=255)
                                                                                                                                                                                                                tACATCTGACCGTTTACGACCTGCCGCTGTAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCANAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGTCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGAc                                                                                    <  2:460353/300‑1 (MQ=255)
                                                                                                                                                                                                                    tCTGACCGGTTACGACCTGCCGCTGTAAGAACTGAAGAACTTCCGTCAGTTGCATTCAAAAACCCCAGGCCACCCGTAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTg                                                                                  <  2:39755/298‑1 (MQ=255)
                                                                                                                                                                                                                            gTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGAAGGCACGCTGGGACTGGGCAAGCTGa                                                                        >  2:555006/1‑300 (MQ=255)
                                                                                                                                                                                                                            gTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCAACTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTACAACGAAGTCTGTTCGCTGGCAGGAACGCTGGGACTGGGCAAGCTGa                                                                        >  2:437974/1‑300 (MQ=255)
                                                                                                                                                                                                                            gTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGNCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGTAGGCACGCTGGGACTGGGCAAGCTGa                                                                        >  2:468816/1‑300 (MQ=255)
                                                                                                                                                                                                                                                        gaactTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTc                                            <  1:3756/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                gTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGTGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCAGGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGa                                      <  1:256856/298‑1 (MQ=255)
                                                                                                                                                                                                                                                                         aTTCGAAAACCCCAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTTGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAAcgg                           <  2:412607/300‑3 (MQ=255)
                                                                                                                                                                                                                                                                                  ccccAGGCCACCCGGAGATTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGt                  >  2:122137/1‑300 (MQ=255)
                                                                                                                                                                                                                                                                                                  gaTTGACTATACGCCATGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATTAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGc  <  1:354522/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                  gaTTGACTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGc  <  1:354317/300‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                      
GACCTTGCCAATGCGATTCGCGCACTCAGTATGGATGCGGTACAAAAAGCCAACTCTGGTCATCCCGGCGCGCCGATGGGCATGGCTGATATTGCCGAAGTGCTGTGGAACGATTTTCTTAAACATAACCCTACCGACCCAACCTGGTATGATCGCGACCGCTTTATTCTTTCCAACGGTCACGCGTCGATGCTGCTCTACAGTTTGCTACATCTGACCGGTTACGACCTGCCGCTGGAAGAACTGAAGAACTTCCGTCAGTTGCATTCGAAAACCCCAGGCCACCCGGAGATTGGCTATACGCCAGGCGTTGAAACCACCACCGGCCCGCTTGGACAAGGTTTGGCGAACGCCGTCGGGCTGGCGATAGCAGAGCGTACACTGGCGGCGCAGTTTAACCAGCCAGACCATGAGATCGTCGATCACTTCACCTATGTGTTTATGGGCGACGGCTGCCTGATGGAAGGTATTTCCCACGAAGTCTGTTCGCTGGCAGGCACGCTGGGACTGGGCAAGCTGATTGGTTTTTACGATCACAACGGTATTTCCATCGACGGTGAAACAGAAGGCTGGTTTACCGACGATACGGC  >  NC_000913/2579648‑2580237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑