Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	65,788	A→T	intergenic (‑8/+67)	polB ← / ← araD	DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	65,788	0	A	T	100.0%	157.9 / NA	45	intergenic (‑8/+67)	polB/araD	DNA polymerase II/L‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (26/19);  total (26/19)

GCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGAT  >  NC_000913/65496‑66079                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                    gcgcTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCCTACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCATTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCa                                                                                                                                                                                                                                                                                              >  1:59507/1‑300 (MQ=255) gcgcTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCa                                                                                                                                                                                                                                                                                              >  1:55785/1‑300 (MQ=255)                                     aCAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTCACCTGCAACGGCCCGTTGTCCGTCGACAGCCAGAAGGAGACTTCTGTCCCTTGAGGGGTGTCCAGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCAAGCTGTAATCCCCTCAAAAAACCAGGCTTGATTATAGACCGGTTTCGt                                                                                                                                                                                                                                                              >  2:608485/1‑296 (MQ=255)                                       aGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGAtt                                                                                                                                                                                                                                                        >  2:587164/1‑300 (MQ=255)                                        gTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCATTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCATGGCTTCGTTTGAtt                                                                                                                                                                                                                                                        >  2:354012/1‑299 (MQ=255)                                              gCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTgg                                                                                                                                                                                                                                                      >  2:152326/1‑295 (MQ=255)                                              gCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCGTGATTATAGCCTGGTTTCGTTTGATTgg                                                                                                                                                                                                                                                      >  2:152314/1‑295 (MQ=255)                                              gCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAAACAGGCTTGATTATAGCCTGGTTTCGTTTGATTgg                                                                                                                                                                                                                                                      >  1:326533/1‑295 (MQ=255)                                              gCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCAAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTgg                                                                                                                                                                                                                                                      >  1:326560/1‑295 (MQ=255)                                                        ggCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCTGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGANGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCNGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATAc                                                                                                                                                                                                                                        <  2:480935/299‑1 (MQ=255)                                                                 tGAAAATCCTTTAACGCCAGCGGTGTCATGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCGGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACt                                                                                                                                                                                                                              <  1:156406/300‑1 (MQ=255)                                                                 tGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACt                                                                                                                                                                                                                              <  1:487335/300‑1 (MQ=255)                                                                                       gtgtCAGGCGAACGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGcc                                                                                                                                                                                                        >  1:33444/1‑300 (MQ=255)                                                                                                                                gcgcGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTACCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATNCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTAGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTttctgcc                                                                                                                                                               >  2:192868/1‑299 (MQ=255)                                                                                                                                         aCCTGATCTGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATATCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTa                                                                                                                                                      <  2:531963/300‑1 (MQ=255)                                                                                                                                         aCCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTa                                                                                                                                                      <  1:92606/300‑1 (MQ=255)                                                                                                                                                     ggAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCATGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTa                                                                                                                                          >  2:422178/1‑300 (MQ=255)                                                                                                                                                                    gACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGTGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAata                                                                                                                           >  1:832/1‑300 (MQ=255)                                                                                                                                                                      ctctTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCATTGTGGGGTGTCCAGCCAGTGTCGGGTTAAGATAAACCCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTAGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCTGGTAACTGCGGCGCTAACTGACGGCAGAATATc                                                                                                                         >  2:408324/1‑300 (MQ=255)                                                                                                                                                                         ttGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGGTTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATccc                                                                                                                       <  1:607209/299‑1 (MQ=255)                                                                                                                                                                              gTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCACAtata                                                                                                                 >  1:273183/1‑300 (MQ=255)                                                                                                                                                                                  aaGCTTAACCTGCAACGGCCCGTTGTCCTTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTTACGGCAGAATATCCCCATATAAGCg                                                                                                             <  1:65947/300‑1 (MQ=255)                                                                                                                                                                                  aaGCGTAACCTGCAACGGCCCGTTGTCCTTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCg                                                                                                             <  1:66474/300‑1 (MQ=255)                                                                                                                                                                                   aGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGa                                                                                                            >  1:395343/1‑300 (MQ=255)                                                                                                                                                                                   aGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCATTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTNATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGa                                                                                                            >  2:464517/1‑300 (MQ=255)                                                                                                                                                                                    gCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGAc                                                                                                           >  1:562407/1‑300 (MQ=255)                                                                                                                                                                                         aCCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGNTGTAATTCCATCAAAAAACCAGGCTTGATTATNGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGAACTCtt                                                                                                      >  2:480580/1‑300 (MQ=255)                                                                                                                                                                                               aaCGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCa                                                                                                <  2:562407/300‑1 (MQ=255)                                                                                                                                                                                                   gCCCGTTGTCCGTCGCCAGCCATAAGGAGACTTCTGTCCCTTGCTGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGTTTTCGTTCGATTGGCTGTGTTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCAc                                                                                               <  2:240468/297‑1 (MQ=255)                                                                                                                                                                                                   gCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGAt                                                                                            <  1:555767/300‑1 (MQ=255)                                                                                                                                                                                                              gTCGCTAGCCAGAAGTAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGGGAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCATGCTTTATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTACCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCAc                                                                                               <  2:341630/286‑1 (MQ=255)                                                                                                                                                                                                              gTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTTTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCGGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGc                                                                                  <  2:628351/299‑1 (MQ=255)                                                                                                                                                                                                               tCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATTGCGTTATGCAc                                                                                <  1:326544/300‑1 (MQ=255)                                                                                                                                                                                                               tCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCAc                                                                                <  1:327223/300‑1 (MQ=255)                                                                                                                                                                                                                      ccagAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTACAGCACGATGGCGTTATGCACTGCATCt                                                                         >  2:62365/1‑300 (MQ=255)                                                                                                                                                                                                                                 cttatGTCCCTTGCGGGGTGTCCCGCCAGTGTAGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTGACTGACGGCAGAATATCCCCATATAAGCGAACTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCAttttt                                                              >  2:118970/5‑300 (MQ=255)                                                                                                                                                                                                                                 cTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGc                                                                                  >  2:232098/1‑280 (MQ=255)                                                                                                                                                                                                                                 cTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGc                                                                                  <  1:232098/280‑1 (MQ=255)                                                                                                                                                                                                                                 cTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCAttttt                                                              >  1:400528/1‑300 (MQ=255)                                                                                                                                                                                                                                 cTTCTGTCCATTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGGTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTAATGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGTGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCAATATAAGCGACCTCTTACAGCACGATGGCGTTATGCACCGCATCTTCGGCAtttt                                                               >  2:157424/1‑299 (MQ=255)                                                                                                                                                                                                                                               ggggTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCaaatg                                                <  1:152326/299‑3 (MQ=255)                                                                                                                                                                                                                                               ggggTGTCCCGCCAGGGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCaaatg                                                <  1:152314/299‑3 (MQ=255)                                                                                                                                                                                                                                                                          aTAAAACCTGCCTGCGCCACGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTAATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAggac                          <  2:373545/294‑4 (MQ=255)                                                                                                                                                                                                                                                                                            aCGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGAt  >  1:108450/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            aCGCTGTAATTCCATCAAAAAACCAGGCTTGATTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTAATGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATTCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCC‑ATGCAAATGGGCCGTGAGAATGGACCAGGACGCCGGGCATTTGCGCTGCATCGAt  >  1:108315/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                    GCGCTTTTCGTAATTCATCAATTGGCGATGGGCGCGACAGTAAAGGCCATACACCGGCTGGCGGTGAAAATCCTTTAACGCCAGCGGTGTCAGGCGAAAGCCTTGTTCACCCTGCAAAATATGCTGAGCGCGGGGAACCTGATCGGCGGGAATAAACGCCACGGACTCTTGCGGTGCAAGCGTAACCTGCAACGGCCCGTTGTCCGTCGCCAGCCAGAAGGAGACTTCTGTCCCTTGCGGGGTGTCCCGCCAGTGTCGGGTTAAGATAAAACCTGCCTGCGCCACGCTGAAAATCCATCAAAAAACCAGGCTTGAGTATAGCCTGGTTTCGTTTGATTGGCTGTGGTTTTATACAGTCATTACTGCCCGTAATATGCCTTCGCGCCATGCTTACGCAGATAGTGTTTATCCAGCAGCGTTTGCTGCATATCCGGTAACTGCGGCGCTAACTGACGGCAGAATATCCCCATATAAGCGACCTCTTCCAGCACGATGGCGTTATGCACCGCATCTTCGGCATTTTTGCCCCATGCAAACGGGCCGTGGGAATGGACCAGAACGCCGGGCATTTGCGCTGCATCGAT  >  NC_000913/65496‑66079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑