Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	83,344	G→A	A62A (GCC→GCT)	leuA ←	2‑isopropylmalate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	83,344	0	G	A	100.0%	253.8 / NA	79	A62A (GCC→GCT)	leuA	2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/36);  total (43/36)

TAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGC‑GGGCGAT‑GGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGAT  >  NC_000913/83052‑83633                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                   tAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAt                                                                                                                                                                                                                                                                                               >  2:610880/1‑298 (MQ=255)         gcTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCATGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCAc                                                                                                                                                                                                                                                                                     <  1:240532/299‑1 (MQ=255)         gcTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGNTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCAc                                                                                                                                                                                                                                                                                     <  2:501614/299‑1 (MQ=255)         gcTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTGCGCAAGAAAATTCAACATCATCGGTGGAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCAc                                                                                                                                                                                                                                                                                     <  1:240733/299‑1 (MQ=255)                       cgcCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTCACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATcacc                                                                                                                                                                                                                                                                      >  1:587734/1‑297 (MQ=255)                       cgcCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATcac                                                                                                                                                                                                                                                                       >  1:587710/1‑297 (MQ=255)                        gcCAGATCGGCAATGGGTGGACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCAc                                                                                                                                                                                                                                                                                     <  2:19904/284‑1 (MQ=255)                           aGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGc                                                                                                                                                                                                                                                                  >  1:166432/1‑300 (MQ=255)                                ggCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATTACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGAATTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGATTGTTTTTAACCTGGC‑GAGCGAT‑GTTTTGCCCTGATTCa                                                                                                                                                                                                                                                                              >  2:163351/1‑283 (MQ=255)                                   aaTGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAagaaa                                                                                                                                                                                                                                                           <  1:500884/299‑3 (MQ=255)                                      gggTGTACGCCCGGCACTCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTCACCTGGCCGAGCGATCGGTTTGCCCTGATTCaaacatcaacaggcgacggga                                                                                                                                                                                                                                                          >  1:219931/1‑282 (MQ=255)                                       ggTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACgggg                                                                                                                                                                                                                                                      <  1:506305/300‑1 (MQ=255)                                        gtgtACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGGT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGa                                                                                                                                                                                                                                                     >  1:587239/1‑300 (MQ=255)                                        gtgtACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAagaaa                                                                                                                                                                                                                                                           >  2:345059/1‑292 (MQ=255)                                        gtgtACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGa                                                                                                                                                                                                                                                     >  1:377664/1‑300 (MQ=255)                                                   gCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGTGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGTGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCAGGCGAAGAAACGGGGAAACCGACTTcc                                                                                                                                                                                                                                          >  2:318404/1‑300 (MQ=255)                                                     a‑TCTTCGCAAGAAACTTCAACATCATCTGTTTACTTACTGGCGCGTTTCACCATATAAATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGTGGGCGGTGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAATGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCGCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCTAAGAAACGGGGAAACCGACTTCCAt                                                                                                                                                                                                                                        <  2:340098/300‑1 (MQ=255)                                                          tCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACg                                                                                                                                                                                                                                    <  1:634163/300‑1 (MQ=255)                                                                     ttCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTT‑GGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATa                                                                                                                                                                                                                        <  1:316338/300‑1 (MQ=255)                                                                           atcatcGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCATGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTc                                                                                                                                                                                                                   <  1:201613/300‑1 (MQ=255)                                                                           atcatcGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTc                                                                                                                                                                                                                   <  1:523601/300‑1 (MQ=255)                                                                                 ggTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTTGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTTGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGTCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGc                                                                                                                                                                                                             <  2:161244/300‑1 (MQ=255)                                                                                 ggTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAggg                                                                                                                                                                                                              <  2:409387/299‑1 (MQ=255)                                                                                 ggTGTAATGACGGGCGCGTTTCACCATAGAGATAACGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGGAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACTTCAACACCCATACGCTCaactgc                                                                                                                                                                                                             <  2:161262/300‑5 (MQ=255)                                                                                  gtgtAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCCTACGCTCAAGGGcc                                                                                                                                                                                                            >  2:434324/1‑300 (MQ=255)                                                                                  gtgtAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGcc                                                                                                                                                                                                            >  1:352471/1‑300 (MQ=255)                                                                                  gtgtAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCAATTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGcc                                                                                                                                                                                                            >  2:434311/1‑300 (MQ=255)                                                                                    gtAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGAACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCa                                                                                                                                                                                                           >  2:590994/1‑299 (MQ=255)                                                                                                       aCCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttct                                                                                                                                                                                       >  1:514188/1‑300 (MQ=255)                                                                                                        ccATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttctt                                                                                                                                                                                      >  2:623043/1‑300 (MQ=255)                                                                                                         cATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttcttt                                                                                                                                                                                     >  1:558217/1‑300 (MQ=255)                                                                                                         cATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAAACTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGTGCAATTTGCAGTTtttcttt                                                                                                                                                                                     >  2:96797/1‑300 (MQ=255)                                                                                                          atatAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttcttt                                                                                                                                                                                     >  2:81232/1‑299 (MQ=255)                                                                                                          atatAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCAACAGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCAAGCGCAATTTGCAGTCtttctttc                                                                                                                                                                                    >  2:82816/1‑300 (MQ=255)                                                                                                                      cgTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCtt                                                                                                                                                                        >  2:334657/1‑300 (MQ=255)                                                                                                                         tCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGTCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGcc                                                                                                                                                                     <  1:427796/300‑1 (MQ=255)                                                                                                                               acttcatCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAAc                                                                                                                                                               >  1:171628/7‑300 (MQ=255)                                                                                                                                cttcatCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGTGAGCTAACGCGCCTACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGCTTTTCTTTCACACTCAAGCTTGCATGTAAc                                                                                                                                                               >  2:15701/6‑299 (MQ=255)                                                                                                                                cttcatCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCAAGTCAAAACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAAc                                                                                                                                                               >  2:15354/6‑299 (MQ=255)                                                                                                                                  tcatCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGcc                                                                                                                                                            <  1:44833/297‑1 (MQ=255)                                                                                                                                    atCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCANCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCt                                                                                                                                                           >  1:128902/2‑299 (MQ=255)                                                                                                                                        agcatgctgcGCAGCTGGGTGGCGATGTACATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCTGCGACTTTCGGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACTCGGCTGTTTTTAACCTGGC‑TAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTc                                                                                                                                                       <  2:7262/295‑1 (MQ=255)                                                                                                                                           gTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTNCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCAAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCATGTAACGCCTGTTCAcc                                                                                                                                                    >  2:194881/1‑299 (MQ=255)                                                                                                                                            tgctgcGCAGCTTGGTTGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCCTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGt                                                                                                                                                  <  2:43910/300‑1 (MQ=255)                                                                                                                                               tgcGCAGCTTGTTGGCGATGTGCATTGGCGAAGTTGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCAc                                                                                                                                                     <  2:352471/294‑1 (MQ=255)                                                                                                                                                    aGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCaa                                                                                                                                           <  1:109180/299‑1 (MQ=255)                                                                                                                                                     gCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAAt                                                                                                                                          >  1:148176/1‑299 (MQ=255)                                                                                                                                                       ttGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGAACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAAtgt                                                                                                                                        <  1:590994/299‑1 (MQ=255)                                                                                                                                                             gCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATc                                                                                                                                  >  2:36793/1‑299 (MQ=255)                                                                                                                                                                  gtgCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAaat                                                                                                                            <  1:368302/300‑1 (MQ=255)                                                                                                                                                                        tGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGAc                                                                                                                      <  1:481302/300‑1 (MQ=255)                                                                                                                                                                                         aGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCCTACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCAAGCCAACAACCATACGCTCAAGGGCCAGCGCAATTTGCNGTTTTTCTTTCACACTCAAGCTTGCCTGTCACGCCTGTTCACCGTCGCGCAATGTGGTACCGAAAATAATGAATTGCTGGCTCATGGttt                                                                                                     >  2:514041/1‑300 (MQ=255)                                                                                                                                                                                         aGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGttt                                                                                                     >  1:402136/1‑300 (MQ=255)                                                                                                                                                                                         aGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGttt                                                                                                     >  2:596761/1‑300 (MQ=255)                                                                                                                                                                                              tGAATACGGAAGGCTGCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCTGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTc                                                                                                <  2:100006/300‑1 (MQ=255)                                                                                                                                                                                               gAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTcc                                                                                               <  1:488738/300‑1 (MQ=255)                                                                                                                                                                                                               ggcggttttCAGGGATTCGGCCGCCACGTCGATATCTTTCGCCACGCAGCGAGCTAACGCGCATACGCTGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGTCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTagag                                                                                <  2:426964/293‑1 (MQ=255)                                                                                                                                                                                                               ggCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTagag                                                                                <  1:163429/299‑1 (MQ=255)                                                                                                                                                                                                               ggCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTATCTTTTagag                                                                                <  1:87027/299‑1 (MQ=255)                                                                                                                                                                                                                gCGACTTTCAGGGATTCGGCCGCCACGGCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCGGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCGCAAGGGCCAGCGCAATTTGCAGTTTTTCGGGCACACTCAAGCTTGCCTGGAACGCCTGTTCACCGTCGCGCAATGTGGTAGCGAAAATAATGACTTGCTGGCTCATGGTGTGGGTCCTTGTCTCTTTTagag                                                                                <  1:514041/298‑1 (MQ=255)                                                                                                                                                                                                                     tttCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAATGCCTGTTCACAGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCCATTTTAGAgcgcat                                                                           >  2:145179/1‑296 (MQ=255)                                                                                                                                                                                                                            gATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCGGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCggg                                                                  <  1:648095/300‑1 (MQ=255)                                                                                                                                                                                                                                          gTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCATGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCCTAAAAAAAccc                                                     >  2:318294/1‑299 (MQ=255)                                                                                                                                                                                                                                             gATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTAGGGCATAAAAAAACCCgcgc                                                 >  2:37273/1‑300 (MQ=255)                                                                                                                                                                                                                                                atCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAAt                                              >  1:190590/1‑300 (MQ=255)                                                                                                                                                                                                                                                    ttctCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCgcgccattgcg                                          <  2:582305/297‑8 (MQ=255)                                                                                                                                                                                                                                                     tctCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTATTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGgcgc                                         >  1:206022/3‑300 (MQ=255)                                                                                                                                                                                                                                                                 gAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTNAACACCCATACGCTCAAGGGCCAGCGCAATTTNCAGTTTTTCTTTCACACTCAAGCTTGCATGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAAACCGCGCAATGGCGCGGGTTTtttgtt                             >  2:489176/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 gAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtt                             >  1:22214/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  aGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgttt                            >  1:515868/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  aGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCAGCGCAATGGCGCGGGTTTtttgttt                            >  2:553318/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  aGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTAGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgttt                            >  2:573466/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  aGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCATGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATCAAAAAACCCGCGAAATGGCGCGGGTTTtttgttt                            >  1:563323/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          cgcaTACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGNTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTAGGGCATAAAAAAACCCGCGAAATGGCGCGGGTTTTTTGTTTGACTGCGt                    >  2:213867/3‑300 (MQ=255)                                                                                                                                                                                                                                                                                      ttttttgAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTTTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATTGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCt             <  2:337690/293‑1 (MQ=255)                                                                                                                                                                                                                                                                                      tGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCTGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATg        <  1:626809/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      tGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATg        <  1:32392/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      tGTTTTTAACCCGGC‑GAGTGAT‑GGTTTGCACGGATTCAAAATCACCCGGCGAGGAACCGTGGAAACCAACTTTCATCCCGTCAACACCCATACGCTTAAGGGCCAGCGCAATTTGTAGTGTTTCTTTCACATTNAAGCTTGCCTGTAACGCCTGTTCACCTTCGCGTAATTTGGTATCGAAAATAATTGCTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAACGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATTGCGCGGGTTTTTTGTTTTACTGCGttctttcctaccc        <  2:213545/300‑13 (MQ=255)                                                                                                                                                                                                                                                                                            tAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGAt  <  1:382189/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                   TAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGC‑GGGCGAT‑GGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTTGACTGCGTGCTGGCTTAATGCTGGAT  >  NC_000913/83052‑83633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑