Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	83,317	A→G	C71C (TGT→TGC)	leuA ←	2‑isopropylmalate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	83,317	0	A	G	100.0%	277.9 / NA	81	C71C (TGT→TGC)	leuA	2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (48/33);  total (48/33)

GAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGC‑GGGCGAT‑GGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTT  >  NC_000913/83025‑83607                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                     gAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATAcgc                                                                                                                                                                                                                                                                                               <  1:32771/300‑1 (MQ=255)   aTGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCAGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATAcgc                                                                                                                                                                                                                                                                                               >  1:439055/1‑298 (MQ=255)     gTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCt                                                                                                                                                                                                                                                                                           >  1:12434/1‑300 (MQ=255)     gTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAAAATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATAc                                                                                                                                                                                                                                                                                                 >  2:277295/1‑294 (MQ=255)     gTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTAGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCAACGTCGATATCTTTCTCCACGCAGCGAGCTCACGCGCATACGCGGCt                                                                                                                                                                                                                                                                                           >  2:277229/1‑300 (MQ=255)                 ggCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCt                                                                                                                                                                                                                                                                                >  1:33351/1‑299 (MQ=255)                       ggCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTggc‑gagc                                                                                                                                                                                                                                                                        <  2:575955/300‑4 (MQ=255)                        gCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTggc‑gagc                                                                                                                                                                                                                                                                        >  2:536521/1‑296 (MQ=255)                            tAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAt                                                                                                                                                                                                                                                                     >  2:610880/1‑298 (MQ=255)                                    gcTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCATGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCAc                                                                                                                                                                                                                                                           <  1:240532/299‑1 (MQ=255)                                    gcTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGNTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCAc                                                                                                                                                                                                                                                           <  2:501614/299‑1 (MQ=255)                                    gcTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTGCGCAAGAAAATTCAACATCATCGGTGGAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCAc                                                                                                                                                                                                                                                           <  1:240733/299‑1 (MQ=255)                                                  cgcCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTCACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATcacc                                                                                                                                                                                                                                            >  1:587734/1‑297 (MQ=255)                                                  cgcCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATcac                                                                                                                                                                                                                                             >  1:587710/1‑297 (MQ=255)                                                   gcCAGATCGGCAATGGGTGGACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCAc                                                                                                                                                                                                                                                           <  2:19904/284‑1 (MQ=255)                                                      aGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGc                                                                                                                                                                                                                                        >  1:166432/1‑300 (MQ=255)                                                           ggCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATTACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGAATTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGATTGTTTTTAACCTGGC‑GAGCGAT‑GTTTTGCCCTGATTCa                                                                                                                                                                                                                                                    >  2:163351/1‑283 (MQ=255)                                                              aaTGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAagaaa                                                                                                                                                                                                                                 <  1:500884/299‑3 (MQ=255)                                                                 gggTGTACGCCCGGCACTCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTCACCTGGCCGAGCGATCGGTTTGCCCTGATTCaaacatcaacaggcgacggga                                                                                                                                                                                                                                >  1:219931/1‑282 (MQ=255)                                                                  ggTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACgggg                                                                                                                                                                                                                            <  1:506305/300‑1 (MQ=255)                                                                   gtgtACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGGT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGa                                                                                                                                                                                                                           >  1:587239/1‑300 (MQ=255)                                                                   gtgtACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAagaaa                                                                                                                                                                                                                                 >  2:345059/1‑292 (MQ=255)                                                                   gtgtACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGa                                                                                                                                                                                                                           >  1:377664/1‑300 (MQ=255)                                                                              gCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGTGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGTGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCAGGCGAAGAAACGGGGAAACCGACTTcc                                                                                                                                                                                                                >  2:318404/1‑300 (MQ=255)                                                                                a‑TCTTCGCAAGAAACTTCAACATCATCTGTTTACTTACTGGCGCGTTTCACCATATAAATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGTGGGCGGTGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAATGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCGCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCTAAGAAACGGGGAAACCGACTTCCAt                                                                                                                                                                                                              <  2:340098/300‑1 (MQ=255)                                                                                     tCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACg                                                                                                                                                                                                          <  1:634163/300‑1 (MQ=255)                                                                                                ttCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTT‑GGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATa                                                                                                                                                                                              <  1:316338/300‑1 (MQ=255)                                                                                                      atcatcGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCATGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTc                                                                                                                                                                                         <  1:201613/300‑1 (MQ=255)                                                                                                      atcatcGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTc                                                                                                                                                                                         <  1:523601/300‑1 (MQ=255)                                                                                                            ggTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTTGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTTGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGTCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGc                                                                                                                                                                                   <  2:161244/300‑1 (MQ=255)                                                                                                            ggTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAggg                                                                                                                                                                                    <  2:409387/299‑1 (MQ=255)                                                                                                            ggTGTAATGACGGGCGCGTTTCACCATAGAGATAACGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGGAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACTTCAACACCCATACGCTCaactgc                                                                                                                                                                                   <  2:161262/300‑5 (MQ=255)                                                                                                             gtgtAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCCTACGCTCAAGGGcc                                                                                                                                                                                  >  2:434324/1‑300 (MQ=255)                                                                                                             gtgtAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGcc                                                                                                                                                                                  >  1:352471/1‑300 (MQ=255)                                                                                                             gtgtAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCAATTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGcc                                                                                                                                                                                  >  2:434311/1‑300 (MQ=255)                                                                                                               gtAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGAACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCa                                                                                                                                                                                 >  2:590994/1‑299 (MQ=255)                                                                                                                                  aCCATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttct                                                                                                                                                             >  1:514188/1‑300 (MQ=255)                                                                                                                                   ccATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttctt                                                                                                                                                            >  2:623043/1‑300 (MQ=255)                                                                                                                                    cATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttcttt                                                                                                                                                           >  1:558217/1‑300 (MQ=255)                                                                                                                                    cATATAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAAACTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGTGCAATTTGCAGTTtttcttt                                                                                                                                                           >  2:96797/1‑300 (MQ=255)                                                                                                                                     atatAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTtttcttt                                                                                                                                                           >  2:81232/1‑299 (MQ=255)                                                                                                                                     atatAGATAGCGCGTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCAACAGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCAAGCGCAATTTGCAGTCtttctttc                                                                                                                                                          >  2:82816/1‑300 (MQ=255)                                                                                                                                                 cgTTCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCtt                                                                                                                                              >  2:334657/1‑300 (MQ=255)                                                                                                                                                    tCGATCACTTCATCCAGCGTGCTGCGCAGCTTGGTGTCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGcc                                                                                                                                           <  1:427796/300‑1 (MQ=255)                                                                                                                                                          acttcatCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAAc                                                                                                                                     >  1:171628/7‑300 (MQ=255)                                                                                                                                                           cttcatCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGTGAGCTAACGCGCCTACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGAGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGCTTTTCTTTCACACTCAAGCTTGCATGTAAc                                                                                                                                     >  2:15701/6‑299 (MQ=255)                                                                                                                                                           cttcatCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCAAGTCAAAACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAAc                                                                                                                                     >  2:15354/6‑299 (MQ=255)                                                                                                                                                             tcatCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGcc                                                                                                                                  <  1:44833/297‑1 (MQ=255)                                                                                                                                                               atCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCANCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCt                                                                                                                                 >  1:128902/2‑299 (MQ=255)                                                                                                                                                                   agcatgctgcGCAGCTGGGTGGCGATGTACATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCTGCGACTTTCGGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACTCGGCTGTTTTTAACCTGGC‑TAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTc                                                                                                                             <  2:7262/295‑1 (MQ=255)                                                                                                                                                                      gTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTNCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCAAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCATGTAACGCCTGTTCAcc                                                                                                                          >  2:194881/1‑299 (MQ=255)                                                                                                                                                                       tgctgcGCAGCTTGGTTGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCCTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGt                                                                                                                        <  2:43910/300‑1 (MQ=255)                                                                                                                                                                          tgcGCAGCTTGTTGGCGATGTGCATTGGCGAAGTTGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCAc                                                                                                                           <  2:352471/294‑1 (MQ=255)                                                                                                                                                                               aGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCaa                                                                                                                 <  1:109180/299‑1 (MQ=255)                                                                                                                                                                                gCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAAt                                                                                                                >  1:148176/1‑299 (MQ=255)                                                                                                                                                                                  ttGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGAACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAAtgt                                                                                                              <  1:590994/299‑1 (MQ=255)                                                                                                                                                                                        gCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATc                                                                                                        >  2:36793/1‑299 (MQ=255)                                                                                                                                                                                             gtgCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAaat                                                                                                  <  1:368302/300‑1 (MQ=255)                                                                                                                                                                                                   tGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGAc                                                                                            <  1:481302/300‑1 (MQ=255)                                                                                                                                                                                                                    aGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCCTACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCAAGCCAACAACCATACGCTCAAGGGCCAGCGCAATTTGCNGTTTTTCTTTCACACTCAAGCTTGCCTGTCACGCCTGTTCACCGTCGCGCAATGTGGTACCGAAAATAATGAATTGCTGGCTCATGGttt                                                                           >  2:514041/1‑300 (MQ=255)                                                                                                                                                                                                                    aGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGttt                                                                           >  2:596761/1‑300 (MQ=255)                                                                                                                                                                                                                    aGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGttt                                                                           >  1:402136/1‑300 (MQ=255)                                                                                                                                                                                                                         tGAATACGGAAGGCTGCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCTGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTc                                                                      <  2:100006/300‑1 (MQ=255)                                                                                                                                                                                                                          gAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTcc                                                                     <  1:488738/300‑1 (MQ=255)                                                                                                                                                                                                                                          ggcggttttCAGGGATTCGGCCGCCACGTCGATATCTTTCGCCACGCAGCGAGCTAACGCGCATACGCTGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGTCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTagag                                                      <  2:426964/293‑1 (MQ=255)                                                                                                                                                                                                                                          ggCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTagag                                                      <  1:163429/299‑1 (MQ=255)                                                                                                                                                                                                                                          ggCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTATCTTTTagag                                                      <  1:87027/299‑1 (MQ=255)                                                                                                                                                                                                                                           gCGACTTTCAGGGATTCGGCCGCCACGGCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCGGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCGCAAGGGCCAGCGCAATTTGCAGTTTTTCGGGCACACTCAAGCTTGCCTGGAACGCCTGTTCACCGTCGCGCAATGTGGTAGCGAAAATAATGACTTGCTGGCTCATGGTGTGGGTCCTTGTCTCTTTTagag                                                      <  1:514041/298‑1 (MQ=255)                                                                                                                                                                                                                                                tttCAGGGATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAATGCCTGTTCACAGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCCATTTTAGAgcgcat                                                 >  2:145179/1‑296 (MQ=255)                                                                                                                                                                                                                                                       gATTCGGCCGCCACGTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCGGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCggg                                        <  1:648095/300‑1 (MQ=255)                                                                                                                                                                                                                                                                     gTCGATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCATGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCCTAAAAAAAccc                           >  2:318294/1‑299 (MQ=255)                                                                                                                                                                                                                                                                        gATATCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTAGGGCATAAAAAAACCCgcgc                       >  2:37273/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           atCTTTCTCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAAt                    >  1:190590/1‑300 (MQ=255)                                                                                                                                                                                                                                                                               ttctCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCgcgccattgcg                <  2:582305/297‑8 (MQ=255)                                                                                                                                                                                                                                                                                tctCCACGCAGCGAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTATTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGgcgc               >  1:206022/3‑300 (MQ=255)                                                                                                                                                                                                                                                                                            gAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTNAACACCCATACGCTCAAGGGCCAGCGCAATTTNCAGTTTTTCTTTCACACTCAAGCTTGCATGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAAACCGCGCAATGGCGCGGGTTTtttgtt   >  2:489176/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                            gAGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgtt   >  1:22214/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             aGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgttt  >  1:515868/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             aGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCAGCGCAATGGCGCGGGTTTtttgttt  >  2:553318/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             aGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTAGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTtttgttt  >  2:573466/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                             aGCTAACGCGCATACGCGGCTGTTTTTAACCTGGC‑GAGCGAT‑GGTTTGCACTGATTCAAAATCACCCGGCGAAGAAACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCATGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATCAAAAAACCCGCGAAATGGCGCGGGTTTtttgttt  >  1:563323/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                     GAATGTTGATGGTGGTGGCACCGGCATTAATCGCCGCTTCGACCACTCGCGCCAGATCGGCAATGGGTGTACGCCCGGCA‑TCTTCGCAAGAAAATTCAACATCATCGGTGTAATTACGGGCGCGTTTCACCATATAGATAGCGCGTTCGATCACCTCGTCCAGCGTGCTGCGCAGCTTGGTGGCGATGTGCATTGGCGAAGTGGCAATAAAGGTATGAATACGGAAGGCTTCGGCGACTTTCAGGGATTCGGCCGCCACGTCGATATCTTTTTCCACGCAGCGAGCTAACGCACATACGCGGCTGTTTTTAACCTGGC‑GGGCGAT‑GGTTTGCACCGATTCAAAATCGCCCGGCGAAGAGACGGGGAAACCGACTTCCATCACGTCAACACCCATACGCTCAAGGGCCAGCGCAATTTGCAGTTTTTCTTTCACACTCAAGCTTGCCTGTAACGCCTGTTCACCGTCGCGCAATGTGGTATCGAAAATAATGACTTGCTGGCTCATGGTTTGGGTCCTTGTCTCTTTTAGAGCGCCTCGCTTCGGGCATAAAAAAACCCGCGCAATGGCGCGGGTTTTTTGTTT  >  NC_000913/83025‑83607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑