Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	80,529	C→G	G112G (GGG→GGC)	leuC ←	3‑isopropylmalate dehydratase subunit LeuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	80,529	0	C	G	100.0%	221.5 / NA	62	G112G (GGG→GGC)	leuC	3‑isopropylmalate dehydratase subunit LeuC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (28/34);  total (28/34)

TTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACAC  >  NC_000913/80243‑80822                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                      ttGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGa                                                                                                                                                                                                                                                                                          >  2:388854/1‑300 (MQ=255)                            gCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGt                                                                                                                                                                                                                                                               <  1:638390/300‑1 (MQ=255)                                 gTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCACATTACGTGGACGATCCACTGATACGGGTGATTCAGGTCATAc                                                                                                                                                                                                                                                          >  1:339040/1‑300 (MQ=255)                                 gTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCACGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATAc                                                                                                                                                                                                                                                          >  2:534118/1‑300 (MQ=255)                                     cgccTGCGCTAACGGTTTTACCGATAATTGACAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTCCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCAGGCAAGGTGACGCACTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCACACAGtt                                                                                                                                                                                                                                                      >  2:191544/1‑300 (MQ=255)                                      gccTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTc                                                                                                                                                                                                                                                     <  1:563767/300‑1 (MQ=255)                                                 cGGTTTTACCGATAATTGCCAGCATGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCACGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAAt                                                                                                                                                                                                                                           >  2:270654/1‑299 (MQ=255)                                                  ggTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTc                                                                                                                                                                                                                                         <  1:407120/300‑1 (MQ=255)                                                                  gCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATc                                                                                                                                                                                                                         <  1:376804/300‑1 (MQ=255)                                                                               cTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCNCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCt                                                                                                                                                                                                            >  2:207312/1‑300 (MQ=255)                                                                               cTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCNCAGACAATGGTCATCCACGGCAAGGTGACGCCCTGTTCAGGGCACATTACGTGGACGATCCCCTGATACGGGTGATTAAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCGTGCATCt                                                                                                                                                                                                            >  2:205772/1‑300 (MQ=255)                                                                                       gTAATGCCCGGCGCGGCTTTGCACTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATAcgc                                                                                                                                                                                                    >  1:456950/1‑300 (MQ=255)                                                                                                ggCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTc                                                                                                                                                                                            <  1:608031/299‑1 (MQ=255)                                                                                                 gcgcTGCTTTGCCCTGGACTTCAATGTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTc                                                                                                                                                                                            <  2:111226/298‑1 (MQ=255)                                                                                                      gCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCa                                                                                                                                                                                      >  2:611461/1‑299 (MQ=255)                                                                                                           gCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCCTTTCACCGAAGGTAtt                                                                                                                                                                                >  2:154757/1‑300 (MQ=255)                                                                                                           gCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCAtt                                                                                                                                                                                >  1:254698/1‑300 (MQ=255)                                                                                                                ggACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGt                                                                                                                                                                           >  1:240508/1‑300 (MQ=255)                                                                                                                              atgttttttGCGCGGCCCTGTTTCAGGGTTTGCGTTACCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGTTGGCGGTATGCGAGTCGCCGCATACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGt                                                                                                                                                              <  2:319856/295‑1 (MQ=255)                                                                                                                              aTGGTTTTTGCGCGGTCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAATGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGt                                                                                                                                                              <  2:338286/299‑1 (MQ=255)                                                                                                                                  tttttGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAgaga                                                                                                                                                         <  2:540635/300‑1 (MQ=255)                                                                                                                                  tttttGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAgaga                                                                                                                                                         <  1:388854/300‑1 (MQ=255)                                                                                                                                            gCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGTTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATc                                                                                                                                               <  2:589137/300‑1 (MQ=255)                                                                                                                                            gCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATc                                                                                                                                               >  2:551349/1‑300 (MQ=255)                                                                                                                                                tGTTTCATGGTTTGCGTTGCCAGTACGTGTTCAACTTCGTAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGNTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATcc                                                                                                                                              <  2:507538/297‑1 (MQ=255)                                                                                                                                                 gTTTCAGGGTTTGCGTTGCCGGTACGTGTTCAACTTAGGAAGTGCCGATACCAAAGGTCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCATGATACGGGTGATTCCGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGGTCAGTTCCTGCATCTGGATCCGCGCAATTTCAACGCAGGCATTAATGTTTTTGGTCTGGGTAGAGACGTTGTGATCCAt                                                                                                                                            >  2:143489/1‑298 (MQ=255)                                                                                                                                                 gTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCTACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCAt                                                                                                                                            >  1:314760/1‑298 (MQ=255)                                                                                                                                                 gTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGTATTAATGTATTTGGTCTGGGTAGAGACGTTGTGATCCAt                                                                                                                                            >  2:132022/1‑298 (MQ=255)                                                                                                                                                 gTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCACTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCAt                                                                                                                                            >  2:143434/1‑298 (MQ=255)                                                                                                                                                  tttCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGt                                                                                                                                         <  1:652033/300‑1 (MQ=255)                                                                                                                                                                 tGCCGGTACGTGTTCAACTTCGTAAGGGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGcc                                                                                                                          <  2:68579/300‑1 (MQ=255)                                                                                                                                                                 tGCCAGTACGTGTTCAACTTCTGAAGTGCCGATACCAAAGGCCGGTGCGCCAAACTCGCCGGGGGTTGCGGTAGGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGTTGACGCCCTGTTCCTGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGcc                                                                                                                          <  2:68580/300‑1 (MQ=255)                                                                                                                                                                 tGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGcc                                                                                                                          <  1:572619/300‑1 (MQ=255)                                                                                                                                                                  gCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCAGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGccc                                                                                                                         >  2:639137/1‑300 (MQ=255)                                                                                                                                                                                aaCTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCg                                                                                                            <  2:622301/299‑1 (MQ=255)                                                                                                                                                                                   ttCGGAAGTGCCGATANCAAAGGCCAGTGCGCCAANCGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGCCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCNGGCTGACGTACCGGGc                                                                                                         <  1:551349/299‑1 (MQ=255)                                                                                                                                                                                            gCCGATACCAAAGGCCAGTGCGCCAGACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGNAAGTTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGgc                                                                                               <  1:138681/300‑1 (MQ=255)                                                                                                                                                                                            gCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGNAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGgc                                                                                               <  1:138537/300‑1 (MQ=255)                                                                                                                                                                                                   ccAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGa                                                                                        >  1:66227/1‑300 (MQ=255)                                                                                                                                                                                                   ccAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTAGGCGCGCAGa                                                                                        >  2:419819/1‑300 (MQ=255)                                                                                                                                                                                                             gTGCGCCAACCGCGCCGTGAGTGGCGGTATGAGAGTCGCCGCAGACAAGGGTCATCCCCGGCAATGTGACTCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATGCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGTTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGTCGACCGTGGGCGCGCAGACCATCGAAc                                                                               <  2:270369/299‑1 (MQ=255)                                                                                                                                                                                                                 gcCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGTAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCt                                                                           >  1:183276/1‑299 (MQ=255)                                                                                                                                                                                                                 gcCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCAAGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCACTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTCCCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCt                                                                           >  2:431273/1‑299 (MQ=255)                                                                                                                                                                                                                             gtgggtggCGGTATGCGAGTCGCCGCAGACAATGGTCATCTCCGGCAATGTGACGCCCTGTTCCTGGCCCATTACGTGGACGATCCCCTGATACGGTTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGGTCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACACCTGCGGTGatg                                                                  <  2:2818/296‑3 (MQ=255)                                                                                                                                                                                                                                    gCGGTATGCGAGTCGCNGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGNAGACCATCGAACGCCTGCNGTGAGGTCACTTCATGc                                                       >  1:538833/1‑300 (MQ=255)                                                                                                                                                                                                                                      ggTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCAc                                                     <  1:18062/300‑1 (MQ=255)                                                                                                                                                                                                                                       gTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCAcc                                                    <  1:534118/300‑1 (MQ=255)                                                                                                                                                                                                                                                  gccgcAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCNAATTCTTTGCAGTTTTTGATCAGTTCCTGCATNTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTc                                         >  2:492763/1‑300 (MQ=255)                                                                                                                                                                                                                                                            aTGGTCATCCCCTGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAAc                               <  1:407826/300‑1 (MQ=255)                                                                                                                                                                                                                                                            aTGGTCATCCCCGGCAAGTTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAAc                               <  1:42534/300‑1 (MQ=255)                                                                                                                                                                                                                                                            aTGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATaa                                >  2:64587/1‑299 (MQ=255)                                                                                                                                                                                                                                                            aTGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAAc                               <  1:5145/300‑1 (MQ=255)                                                                                                                                                                                                                                                            aTGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAAc                               <  2:630453/300‑1 (MQ=255)                                                                                                                                                                                                                                                            aTGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGGGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAAc                               <  1:42483/300‑1 (MQ=255)                                                                                                                                                                                                                                                              ggTCATCCCCTGCAAGGTGACGCCCTGTTCCGTGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAg                             <  1:170883/300‑1 (MQ=255)                                                                                                                                                                                                                                                              ggTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGTTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTATAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAg                             <  1:171686/300‑1 (MQ=255)                                                                                                                                                                                                                                                              ggTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAg                             <  1:83625/300‑1 (MQ=255)                                                                                                                                                                                                                                                              ggTCATCCCCGGCAAGGTGACGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATGCAGTTCAGACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAg                             <  1:26426/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                  cGCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCtt         >  1:372923/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   gCCCTGTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTc        >  1:484341/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        gTTCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTaca   >  1:13722/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         ttCCGGGCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTacac  <  1:270654/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                      TTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAGGGTTTGCGTTGCCAGTACGTGTTCAACTTCGGAAGTGCCGATACCAAAGGCCAGTGCGCCAAACGCGCCGTGGGTGGCGGTATGCGAGTCGCCGCAGACAATGGTCATCCCCGGCAAGGTGACGCCCTGTTCCGGCCCCATTACGTGGACGATCCCCTGATACGGGTGATTCAGGTCATACAGTTCGACGCCAAATTCTTTGCAGTTTTTGATCAGTTCCTGCATCTGGATACGCGCCATTTCACCGCAGGCATTAATGTCTTTGGTCTGGGTAGAGACGTTGTGATCCATGGTAGCGAAGGTTTTGCCCGGCTGACGTACCGGGCGACCGTGGGCGCGCAGACCATCGAACGCCTGCGGTGAGGTCACTTCATGCACCAGGTGGCGGTCGATATATAACAGTGGGGTTTCGTTTTCGGCTTCGTACAC  >  NC_000913/80243‑80822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑