Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	72,751	A→G	D59D (GAT→GAC)	thiQ ←	thiamine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	72,751	0	A	G	100.0%	207.7 / NA	60	D59D (GAT→GAC)	thiQ	thiamine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/23);  total (37/23)

GTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGACAACGCGCTAACGCCACTCGCTGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCCCCTGCTGTACCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAA  >  NC_000913/72462‑73043                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                     gTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATTTGGCGGGCGATAGCGTGCATTTTCTACTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAAGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGAAGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTc                                                                                                                                                                                                                                                                                             >  2:338287/1‑299 (MQ=255)             aTAAAGTCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAAcagat                                                                                                                                                                                                                                                                                  >  2:135554/1‑294 (MQ=255)             aTAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATGGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCAGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCgct                                                                                                                                                                                                                                                                                  >  2:136141/1‑298 (MQ=255)             aTAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTACTGCTGTGTCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACAGGACGGCGTGACGGCGGCGTCGTGGTGTGGTCAACGCCGTCGATAGTCAGCGAACCgct                                                                                                                                                                                                                                                                                  >  2:136916/1‑298 (MQ=255)                         gTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTTGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGa                                                                                                                                                                                                                                                                     <  2:283462/299‑1 (MQ=255)                          ttCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGNTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGaaa                                                                                                                                                                                                                                                                   <  2:194574/300‑1 (MQ=255)                                caCCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGc                                                                                                                                                                                                                                                             >  1:396623/2‑300 (MQ=255)                                  ccAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGa                                                                                                                                                                                                                                                           >  1:424554/1‑300 (MQ=255)                                            cgcTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTca                                                                                                                                                                                                                                                  >  1:112901/1‑299 (MQ=255)                                               tAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCACCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTcagcag                                                                                                                                                                                                                                              <  2:365077/300‑1 (MQ=255)                                                      acgcgctgtcgctgACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACtt                                                                                                                                                                                                                                       <  1:182264/297‑1 (MQ=255)                                                             gtcgctgACCGCCGGAAAGCTCGCCAGGTAACCGCGCAATTAAATTATCAATCCACATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCAGGATTTAGCACCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCAGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGCAAACCGGCGATCAAGTTCAGCAGGGTACTTTTAcct                                                                                                                                                                                                                                 >  2:325921/1‑298 (MQ=255)                                                                                    ccaggTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGTGTGCATTTTCTACTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGcc                                                                                                                                                                                                         >  2:454705/4‑300 (MQ=255)                                                                                       ggTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCAcc                                                                                                                                                                                                      <  1:158124/300‑1 (MQ=255)                                                                                                               aTCCCCATCTGGCGGGCGATGGCGTGCATTTTCTACTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCCCCTGCTCGCCGCGTTCTACCGTTaa                                                                                                                                                                               >  2:548394/1‑299 (MQ=255)                                                                                                               aTCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTaa                                                                                                                                                                               >  2:548387/1‑299 (MQ=255)                                                                                                                        tGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAAc                                                                                                                                                                       <  1:609574/298‑1 (MQ=255)                                                                                                                              ggggtAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCAAGCCCGATGTTCTGCGCGACCGTCAGGTGGCTGAACAGGTTGTTTTCACGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCGGCGAACCGCTGGCTGGCGTCAGAACAACGGTGATCAAGTTCAGCAGGGTACCTTTCCCTGCGCCGATTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTGCAGCTAAGCTAAAACGCATGGGc                                                                                                                                                               >  2:320260/5‑300 (MQ=255)                                                                                                                              gcgATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCAGGATTTAGCACCAGCCCGATGTTCTGCGCGACCGCCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCCGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCACGAGGATCGCCAACCGCTCGCCACGTTCTACCGTTAAGCTaaa                                                                                                                                                                         >  2:320479/1‑290 (MQ=255)                                                                                                                                               ctccTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCa                                                                                                                                              >  2:330224/3‑300 (MQ=255)                                                                                                                                                  ctgctgTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCAAGGt                                                                                                                                           >  2:579180/1‑300 (MQ=255)                                                                                                                                                        tgccGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCAATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATAtc                                                                                                                                     <  2:317021/298‑1 (MQ=255)                                                                                                                                                              gTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCAAGGTGATAtcagtca                                                                                                                                >  2:345892/1‑299 (MQ=255)                                                                                                                                                               ttCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATcagtcagt                                                                                                                              >  1:452948/1‑300 (MQ=255)                                                                                                                                                                     ttCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGNGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAAc                                                                                                                        >  2:195832/1‑300 (MQ=255)                                                                                                                                                                      tCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACa                                                                                                                       <  1:602278/300‑1 (MQ=255)                                                                                                                                                                               gATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGccc                                                                                                               >  1:452209/1‑299 (MQ=255)                                                                                                                                                                               gATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGNGTGATCAACGCCGTCGATAGTCAGCGAACCGCNGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGcccc                                                                                                              >  2:482796/1‑300 (MQ=255)                                                                                                                                                                                aTTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTTGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGcccc                                                                                                              >  1:601224/1‑299 (MQ=255)                                                                                                                                                                                           gCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCa                                                                                                  >  2:531290/1‑300 (MQ=255)                                                                                                                                                                                             ccGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACCTCTAGCCCAGGTAGTTTTTAAAt                                                                                                >  2:190088/1‑300 (MQ=255)                                                                                                                                                                                                               gTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGTTAGTTTTTCAATCACGGTAAACAGCAGaaa                                                                              <  1:63012/300‑1 (MQ=255)                                                                                                                                                                                                                tCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCACGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGaaaa                                                                             >  2:358231/1‑300 (MQ=255)                                                                                                                                                                                                                   ggTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACag                                                                          <  2:424554/300‑1 (MQ=255)                                                                                                                                                                                                                     tgacTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTAACGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTAACCCCGGTCGTTTTTCAATCACGGTAAACAGCAGAAAACaga                                                                         >  2:530990/4‑299 (MQ=255)                                                                                                                                                                                                                          gAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCTAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGagtagca                                                                   >  1:401640/1‑300 (MQ=255)                                                                                                                                                                                                                           aaCAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGagtagcag                                                                  <  1:507627/300‑1 (MQ=255)                                                                                                                                                                                                                               ggTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGa                                                              >  1:397072/1‑300 (MQ=255)                                                                                                                                                                                                                                gttgttTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGagtagcagc                                                                 <  1:266419/296‑1 (MQ=255)                                                                                                                                                                                                                                      ttcCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAAcg                                                       <  1:491675/299‑1 (MQ=255)                                                                                                                                                                                                                                             aaCAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGTTGATATCAGTCAGTTTTAACATCTCGCCCCGTTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGAc                                                <  2:350867/300‑1 (MQ=255)                                                                                                                                                                                                                                                       acacCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTcc                                      <  2:577694/300‑1 (MQ=255)                                                                                                                                                                                                                                                       acacCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTAAGTTTTAACATCTCGCCCCGGTAGTTTTTAAATCACGGTAAACAGCAGAGAACAGAGTAGAAGCAGAAt                                                            >  2:101025/1‑278 (MQ=255)                                                                                                                                                                                                                                                            ggACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCt                                 <  1:568292/300‑1 (MQ=255)                                                                                                                                                                                                                                                            ggACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCt                                 >  2:640887/1‑300 (MQ=255)                                                                                                                                                                                                                                                             gactgcgtgacggcttcttcgtggtgtgATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCNGCAGGGTACTTTTACCTTCTCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCt                                 <  2:511159/276‑1 (MQ=255)                                                                                                                                                                                                                                                                 gCGTGACAGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTCACGCGGTGACCGCGCCGTCCTGGCTGcaat                            >  2:306377/1‑297 (MQ=255)                                                                                                                                                                                                                                                                            gcgtcgtggtttgATCAACGCCGTCGATAGTCAGCGGACCGCTGGCTGGCGTCAGAAAACCGTCGATCAAATTCAGCAGGGTACTTTTACCTGCGCCGCTTTGCCCTAGGATCGCCACCTGCTCGCTGCGGTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGTTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAAtt                 <  2:145357/289‑1 (MQ=255)                                                                                                                                                                                                                                                                            gcgtcgtggtgtgATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCTAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGTTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAAtt                 <  2:145471/292‑1 (MQ=255)                                                                                                                                                                                                                                                                              gtcgtggtgtgATCAACGCCGTCGATAGTCAGCGAACCGCTTGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGGTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGTTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAAtt                 <  2:146211/292‑1 (MQ=255)                                                                                                                                                                                                                                                                              gtcgtggtgtgATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTNAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGTGGTGACCGCGCCGTCCTGGCTGCGATAGGAAACAAttt                >  2:212676/7‑299 (MQ=255)                                                                                                                                                                                                                                                                              gtcgtggtgtgATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTNAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCAAGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAAttt                >  2:212622/7‑299 (MQ=255)                                                                                                                                                                                                                                                                              gtcgtggtgtgATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAAttt                >  1:419374/7‑299 (MQ=255)                                                                                                                                                                                                                                                                                   ggtgtgATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGt          >  1:305933/2‑300 (MQ=255)                                                                                                                                                                                                                                                                                        gATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAgaga     >  1:417975/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        gATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCAAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAAAAGAGTAGCAGCAGAATTAACGCGGTGACCGTGTCGTCCTGGCTGCGATAGGGGCCAATTTGCTGGTCgaga     >  2:18325/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        gATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCAGCCTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAACCAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGAc                                                >  2:66046/1‑257 (MQ=255)                                                                                                                                                                                                                                                                                            aaCGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGNGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTTGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGTTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATaa  <  1:135554/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                            aaCGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGNGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATaa  <  1:136141/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                            aaCGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGAGCAAGTTCAGCAGGGTACTTTTACCTGNGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGAATTAACGCGGTGACCGCGCCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATaa  <  1:136916/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                     GTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGACAACGCGCTAACGCCACTCGCTGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCCCCTGCTGTACCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGAATTAACGCGGTGACCGCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAA  >  NC_000913/72462‑73043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑