Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	72,691	C→T	E79E (GAG→GAA)	thiQ ←	thiamine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	72,691	0	C	T	100.0%	190.6 / NA	52	E79E (GAG→GAA)	thiQ	thiamine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/23);  total (29/23)

GTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGACAACGCGCTAACGCCACTCGCTGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCCCCTGCTGTACCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGA  >  NC_000913/72407‑72983                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                     gTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCACGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATc                                                                                                                                                                                                                                                                                       >  2:56841/1‑300 (MQ=255)           gCGTCAGCATCTCCTTACGTAACGCCGGATTGAGCGCAGAGAACGGTTCAGCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTTTCGCTGACCGCCGGAAAGCTCGCCATTTAACCGCGCCATTAAGTTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACTGTCAGGTGACTGAACAGGTTGTTTTCTTGAAACAGCATCGACACCGGAc                                                                                                                                                                                                                                                                             <  1:203016/300‑1 (MQ=255)           gCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGAc                                                                                                                                                                                                                                                                             <  1:203009/300‑1 (MQ=255)           gCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGGAACCGCGCCATTAAATTATCAATCCCCATCNGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTNCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGAc                                                                                                                                                                                                                                                                             <  2:490889/300‑1 (MQ=255)                      tcccgACGTAACGCCGAATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGAcgg                                                                                                                                                                                                                                                                   <  1:215276/295‑1 (MQ=255)                      tcCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGAcggc                                                                                                                                                                                                                                                                  <  1:75200/300‑1 (MQ=255)                                  gCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTTTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTTGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACggcggcgtcgtggtg                                                                                                                                                                                                                                                      <  2:41970/300‑10 (MQ=255)                                  gCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACggcggcgtcgtggtg                                                                                                                                                                                                                                                      <  2:426119/300‑10 (MQ=255)                                     ggATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGa                                                                                                                                                                                                                                                   >  1:577694/1‑300 (MQ=255)                                       aTCGAGCGCAGAGAAGGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATc                                                                                                                                                                                                                                                 <  1:61547/300‑1 (MQ=255)                                        tCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGTCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATc                                                                                                                                                                                                                                                 <  2:18003/299‑1 (MQ=255)                                        tCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATca                                                                                                                                                                                                                                                <  1:598937/300‑2 (MQ=255)                                              gcAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGCCAACGCGCTAACGCCACGCGCTGTCACTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCTGAGTGAGCCCTAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGc                                                                                                                                                                                                                                            <  2:257427/298‑1 (MQ=255)                                                        gTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATTTGGCGGGCGATAGCGTGCATTTTCTACTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAAGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGAAGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTc                                                                                                                                                                                                                                 >  2:338287/1‑299 (MQ=255)                                                                    aTAAAGTCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAAcagat                                                                                                                                                                                                                      >  2:135554/1‑294 (MQ=255)                                                                    aTAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATGGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCAGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCgct                                                                                                                                                                                                                      >  2:136141/1‑298 (MQ=255)                                                                    aTAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTACTGCTGTGTCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACAGGACGGCGTGACGGCGGCGTCGTGGTGTGGTCAACGCCGTCGATAGTCAGCGAACCgct                                                                                                                                                                                                                      >  2:136916/1‑298 (MQ=255)                                                                                gTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTTGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGa                                                                                                                                                                                                         <  2:283462/299‑1 (MQ=255)                                                                                 ttCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGNTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGaaa                                                                                                                                                                                                       <  2:194574/300‑1 (MQ=255)                                                                                       caCCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGc                                                                                                                                                                                                 >  1:396623/2‑300 (MQ=255)                                                                                         ccAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGa                                                                                                                                                                                               >  1:424554/1‑300 (MQ=255)                                                                                                   cgcTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTca                                                                                                                                                                                      >  1:112901/1‑299 (MQ=255)                                                                                                      tAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCACCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTcagcag                                                                                                                                                                                  <  2:365077/300‑1 (MQ=255)                                                                                                             acgcgctgtcgctgACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACtt                                                                                                                                                                           <  1:182264/297‑1 (MQ=255)                                                                                                                    gtcgctgACCGCCGGAAAGCTCGCCAGGTAACCGCGCAATTAAATTATCAATCCACATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCAGGATTTAGCACCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCAGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGCAAACCGGCGATCAAGTTCAGCAGGGTACTTTTAcct                                                                                                                                                                     >  2:325921/1‑298 (MQ=255)                                                                                                                                           ccaggTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGTGTGCATTTTCTACTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGcc                                                                                                                                             >  2:454705/4‑300 (MQ=255)                                                                                                                                              ggTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCAcc                                                                                                                                          <  1:158124/300‑1 (MQ=255)                                                                                                                                                                      aTCCCCATCTGGCGGGCGATGGCGTGCATTTTCTACTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCCCCTGCTCGCCGCGTTCTACCGTTaa                                                                                                                   >  2:548394/1‑299 (MQ=255)                                                                                                                                                                      aTCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTaa                                                                                                                   >  2:548387/1‑299 (MQ=255)                                                                                                                                                                               tGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAAc                                                                                                           <  1:609574/298‑1 (MQ=255)                                                                                                                                                                                     ggggtAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCAAGCCCGATGTTCTGCGCGACCGTCAGGTGGCTGAACAGGTTGTTTTCACGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCGGCGAACCGCTGGCTGGCGTCAGAACAACGGTGATCAAGTTCAGCAGGGTACCTTTCCCTGCGCCGATTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTGCAGCTAAGCTAAAACGCATGGGc                                                                                                   >  2:320260/5‑300 (MQ=255)                                                                                                                                                                                     gcgATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCAGGATTTAGCACCAGCCCGATGTTCTGCGCGACCGCCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCCGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCACGAGGATCGCCAACCGCTCGCCACGTTCTACCGTTAAGCTaaa                                                                                                             >  2:320479/1‑290 (MQ=255)                                                                                                                                                                                                      ctccTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCa                                                                                  >  2:330224/3‑300 (MQ=255)                                                                                                                                                                                                         ctgctgTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCAAGGt                                                                               >  2:579180/1‑300 (MQ=255)                                                                                                                                                                                                               tgccGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCAATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATAtc                                                                         <  2:317021/298‑1 (MQ=255)                                                                                                                                                                                                                     gTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCAAGGTGATAtcagtca                                                                    >  2:345892/1‑299 (MQ=255)                                                                                                                                                                                                                      ttCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATcagtcagt                                                                  >  1:452948/1‑300 (MQ=255)                                                                                                                                                                                                                            ttCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGNGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAAc                                                            >  2:195832/1‑300 (MQ=255)                                                                                                                                                                                                                             tCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACa                                                           <  1:602278/300‑1 (MQ=255)                                                                                                                                                                                                                                      gATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGccc                                                   >  1:452209/1‑299 (MQ=255)                                                                                                                                                                                                                                      gATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGNGTGATCAACGCCGTCGATAGTCAGCGAACCGCNGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGcccc                                                  >  2:482796/1‑300 (MQ=255)                                                                                                                                                                                                                                       aTTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTTGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGcccc                                                  >  1:601224/1‑299 (MQ=255)                                                                                                                                                                                                                                                  gCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCa                                      >  2:531290/1‑300 (MQ=255)                                                                                                                                                                                                                                                    ccGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACCTCTAGCCCAGGTAGTTTTTAAAt                                    >  2:190088/1‑300 (MQ=255)                                                                                                                                                                                                                                                                      gTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGTTAGTTTTTCAATCACGGTAAACAGCAGaaa                  <  1:63012/300‑1 (MQ=255)                                                                                                                                                                                                                                                                       tCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCACGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGaaaa                 >  2:358231/1‑300 (MQ=255)                                                                                                                                                                                                                                                                          ggTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACag              <  2:424554/300‑1 (MQ=255)                                                                                                                                                                                                                                                                            tgacTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTAACGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTAACCCCGGTCGTTTTTCAATCACGGTAAACAGCAGAAAACaga             >  2:530990/4‑299 (MQ=255)                                                                                                                                                                                                                                                                                 gAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCTAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGagtagca       >  1:401640/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                  aaCAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGagtagcag      <  1:507627/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                      ggTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGTAGCAGCAGa  >  1:397072/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                       gttgttTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAGTTCAGCAGGGTACTTTTACCTGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCTACCGTTAAGCTAAAACGCATCGGCAAATGTTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATCTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGagtagcagc     <  1:266419/296‑1 (MQ=255)                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                                     GTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGACAACGCGCTAACGCCACTCGCTGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCCCCTGCTGTACCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAAACCGGCGATCAAATTCAGCAGGGTACTTTTACCCGCGCCGCTTGGCCCGAGGATCGCCACCTGCTCGCCGCGTTCCACCGTTAAGCTAAAACGCATCGGCAAATGGTGGTAAAGCCAGGTGATATCAGTCAGTTTTAACATTTCGCCCCGGTAGTTTTTCAATCACGGTAAACAGCAGAAAACAGAGCAGCAGCAGA  >  NC_000913/72407‑72983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑