Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	72,493	T→C	V145V (GTA→GTG)	thiQ ←	thiamine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	72,493	0	T	C	100.0%	172.9 / NA	49	V145V (GTA→GTG)	thiQ	thiamine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/23);  total (26/23)

ACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGACAACGCGCTAACGCCACTCGCTGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCCCCTGCTGTACCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAA  >  NC_000913/72207‑72786                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                      acgtcgcATCTGACAGTGCATACTAACCCGTAATCCCCAATAGTGCTTAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCGCCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAGCGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAAcgc                                                                                                                                                                                                                                                                                           <  2:350564/295‑1 (MQ=255)               aGTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCATGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACgcgc                                                                                                                                                                                                                                                                            <  2:549075/300‑1 (MQ=255)                gTGCATACTAACCCGTAATCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTACTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACgcgc                                                                                                                                                                                                                                                                            >  1:365077/1‑299 (MQ=255)                                 aTCCCCAATAGTGCTGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCTTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGa                                                                                                                                                                                                                                                           >  2:95376/1‑299 (MQ=255)                                                     aCTCGCCTTACCGCTCAACAACTCATTGGTCCTACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGNTCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACcg                                                                                                                                                                                                                                         <  2:517141/297‑1 (MQ=255)                                                                                 gtcttACCCTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTggc                                                                                                                                                                                                            >  1:522463/5‑298 (MQ=255)                                                                                         cTGCCAGGCGATGCGCCCGTCGGCGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTACTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGc                                                                                                                                                                                                  >  2:602278/1‑300 (MQ=255)                                                                                                           gtcgtcGACCACCACCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACTCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCctgc                                                                                                                                                                                  <  2:526013/298‑1 (MQ=255)                                                                                                                 gaccaccacCGAGCGCGTGGCGATCCGCGCGGCATCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCATGACGTAACGACGGATCGAGTGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTAGCTGACCGCCGGAAAGCTCGCCAGGTACCCGCGCCATTAAATTATCCATCCCCATCTGGCGGGCGATAGTGTGCATTTTCTCCTGCTGTGCcgc                                                                                                                                                                          >  2:63012/8‑300 (MQ=255)                                                                                                                                                  aTCTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGcc                                                                                                                                          <  1:553331/299‑1 (MQ=255)                                                                                                                                                    cTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCGTTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTTTGCCACGTTTAGTTTCAATCCCGGATTTAGCCCCAGcc                                                                                                                                          >  2:182264/1‑297 (MQ=255)                                                                                                                                                    cTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCCATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGa                                                                                                                                       >  1:145471/1‑300 (MQ=255)                                                                                                                                                    cTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCNCCATCTGGCGGGCGATAGCGTGCATTTTCTCATGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGa                                                                                                                                       >  1:476253/1‑300 (MQ=255)                                                                                                                                                    cTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGTGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCACGa                                                                                                                                       >  2:266419/1‑300 (MQ=255)                                                                                                                                                    cTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGa                                                                                                                                       >  1:88322/1‑300 (MQ=255)                                                                                                                                                    cTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTACTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGa                                                                                                                                       >  1:146211/1‑300 (MQ=255)                                                                                                                                                    cTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTACTGACGTAACGCCGGATCGAGCGCAGNGAACGGTTCATCGAGCAATAAAATCGGCTGTTNGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAACCCACATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGa                                                                                                                                       >  2:491675/1‑300 (MQ=255)                                                                                                                                                    cTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTACTGACGTAACGCCGGATCGAGCGCAGGGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGa                                                                                                                                       >  2:568292/1‑300 (MQ=255)                                                                                                                                                    cTTCCACACTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTACTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGACAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGTCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGa                                                                                                                                       >  1:145357/1‑300 (MQ=255)                                                                                                                                                         acacTGTGTGACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTc                                                                                                                                  <  2:595008/297‑1 (MQ=255)                                                                                                                                                             tgtgtgACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATCTTCtgc                                                                                                                               <  2:522463/299‑2 (MQ=255)                                                                                                                                                              gtgtgACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCtgcgc                                                                                                                             >  1:317021/5‑297 (MQ=255)                                                                                                                                                              gtgtgACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAANCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCtgcgc                                                                                                                             >  2:516586/5‑297 (MQ=255)                                                                                                                                                              gtgtgACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAANCCCCATCTGGCGGGCGATAGCGTGCATTTTCTACTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCtgcgc                                                                                                                             >  2:507627/5‑297 (MQ=255)                                                                                                                                                              gtgtgACACCATCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTACTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCtgcgc                                                                                                                             >  1:350867/5‑297 (MQ=255)                                                                                                                                                              gtgtgACACCATCAATAACGTCATTTCTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTACTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTACTGCTGTGCCGCGTTCCGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCtgcgc                                                                                                                             >  1:511159/5‑297 (MQ=255)                                                                                                                                                                        aTCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAgg                                                                                                                   <  1:423607/300‑1 (MQ=255)                                                                                                                                                                         tCAATAACGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCAAGGTAACCGCGCCATTAAATTATCAATCCCAATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTTAGTTTCAATTCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGt                                                                                                                  >  2:70703/1‑300 (MQ=255)                                                                                                                                                                            ataacGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTgac                                                                                                               <  1:70703/296‑3 (MQ=255)                                                                                                                                                                                  tcgttttttGCTGCTGGCAGCTCGTGCTCATCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAAGCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAAc                                                                                                          <  2:79110/296‑1 (MQ=255)                                                                                                                                                                                                         gTGCTCACCAGCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCACGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATc                                                                                  >  2:56841/1‑300 (MQ=255)                                                                                                                                                                                                                   gCGTCAGCATCTCCTTACGTAACGCCGGATTGAGCGCAGAGAACGGTTCAGCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTTTCGCTGACCGCCGGAAAGCTCGCCATTTAACCGCGCCATTAAGTTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACTGTCAGGTGACTGAACAGGTTGTTTTCTTGAAACAGCATCGACACCGGAc                                                                        <  1:203016/300‑1 (MQ=255)                                                                                                                                                                                                                   gCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGAc                                                                        <  1:203009/300‑1 (MQ=255)                                                                                                                                                                                                                   gCGTCAGCATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGGAACCGCGCCATTAAATTATCAATCCCCATCNGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTNCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGAc                                                                        <  2:490889/300‑1 (MQ=255)                                                                                                                                                                                                                              tcccgACGTAACGCCGAATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGAcgg                                                              <  1:215276/295‑1 (MQ=255)                                                                                                                                                                                                                              tcCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGAcggc                                                             <  1:75200/300‑1 (MQ=255)                                                                                                                                                                                                                                          gCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTTTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTTGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACggcggcgtcgtggtg                                                 <  2:41970/300‑10 (MQ=255)                                                                                                                                                                                                                                          gCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACggcggcgtcgtggtg                                                 <  2:426119/300‑10 (MQ=255)                                                                                                                                                                                                                                             ggATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGa                                              >  1:577694/1‑300 (MQ=255)                                                                                                                                                                                                                                               aTCGAGCGCAGAGAAGGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATc                                            <  1:61547/300‑1 (MQ=255)                                                                                                                                                                                                                                                tCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGTCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATc                                            <  2:18003/299‑1 (MQ=255)                                                                                                                                                                                                                                                tCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATca                                           <  1:598937/300‑2 (MQ=255)                                                                                                                                                                                                                                                      gcAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGCCAACGCGCTAACGCCACGCGCTGTCACTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCTGAGTGAGCCCTAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGc                                       <  2:257427/298‑1 (MQ=255)                                                                                                                                                                                                                                                                gTTCATCGAGCAATAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATTTGGCGGGCGATAGCGTGCATTTTCTACTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAAGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGAAGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTc                            >  2:338287/1‑299 (MQ=255)                                                                                                                                                                                                                                                                            aTAAAGTCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAAcagat                 >  2:135554/1‑294 (MQ=255)                                                                                                                                                                                                                                                                            aTAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATGGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCAGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCgct                 >  2:136141/1‑298 (MQ=255)                                                                                                                                                                                                                                                                            aTAAAATCGGCTGTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTACTGCTGTGTCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACAGGACGGCGTGACGGCGGCGTCGTGGTGTGGTCAACGCCGTCGATAGTCAGCGAACCgct                 >  2:136916/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                        gTTCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTTGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGa    <  2:283462/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                         ttCGCGCACCAGACAACGCGCTAACGCCACGCGCTGTCGCTGACCGCCGGAAAGCTCGCCAGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCTCCTGCTGTGCCGCGNTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGCGCGACCGTCAGGTGACTGAACAGGTTGTTTTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCGTCGTGGTGTGATCAACGCCGTCGATAGTCAGCGAACCGCTGGCTGGCGTCAGaaa  <  2:194574/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                      ACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAATAGTGCCGAAGCACTCGCCTTACCGCTCAACAACTCATTGGTCATACCCTGCCAGGCGATGCGCCCGTCGGCGACTACTACCGAGCGCGTGGCGATCCGCGCCGCATCTTCCACGCTGTGCGACACCATCAATAGCGTCATTTTTTGCTGCTGGCAGCTCGTGCTCACCAGCGTCAACATCTCCTGACGTAACGCCGGATCGAGCGCAGAGAACGGTTCATCGAGCAATAAAATCGGCTGTTCGCGTACCAGACAACGCGCTAACGCCACTCGCTGTCGCTGACCGCCGGAAAGCTCGCCCGGTAACCGCGCCATTAAATTATCAATCCCCATCTGGCGGGCGATAGCGTGCATTTTCCCCTGCTGTACCGCGTTCAGTTTCAATCCCGGATTTAGCCCCAGCCCGATGTTCTGTGCGACCGTCAGGTGGCTGAACAGGTTGTTCTCCTGAAACAGCATCGACACCGGACGGCGTGACGGCGGCATAGTTGTGTGATCTACGCCATCGATAGTCAGCGAACCGCTGGCTGGCGTCAGAAA  >  NC_000913/72207‑72786

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑