Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	71,968	C→T	D206D (GAC→GAT)	yabI →	DedA family protein YabI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	71,968	0	C	T	100.0%	159.5 / NA	48	D206D (GAC→GAT)	yabI	DedA family protein YabI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/30);  total (18/30)

AGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAAT  >  NC_000913/71675‑72247                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                        aGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGttta                                                                                                                                                                                                                                                                                   >  2:281160/1‑299 (MQ=255) aGCATGTTCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCAACGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGttta                                                                                                                                                                                                                                                                                   >  2:280422/1‑299 (MQ=255)    aTGTTCACCATTCTGGNCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTTGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGNTTATGGCGCAGCGGTAAANCAACCGATCGTTTAAGt                                                                                                                                                                                                                                                                                >  1:552476/1‑300 (MQ=255)        tcaacATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCAGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGNTGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCatt                                                                                                                                                                                                                                                                            >  1:125901/5‑300 (MQ=255)        tCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGNCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCatt                                                                                                                                                                                                                                                                            >  1:126269/1‑300 (MQ=255)        tCACCATTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCAGGTGGCAAAGTTCATTACGCAGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTACTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCatt                                                                                                                                                                                                                                                                            >  2:436582/1‑300 (MQ=255)             aTTCTGGTCGGTCGCTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCattatt                                                                                                                                                                                                                                                                         >  2:428726/1‑298 (MQ=255)                             ttgttgGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTttgt                                                                                                                                                                                                                                                       <  1:168813/300‑1 (MQ=255)                                                  tGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGAtt                                                                                                                                                                                                                                   <  1:522510/299‑1 (MQ=255)                                                     tGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGACGTTGATTTCt                                                                                                                                                                                                                                >  2:29514/1‑299 (MQ=255)                                                         aaTGGTGGCGGGAATGCTGGAGCTGCCGTTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGcc                                                                                                                                                                                                                             <  2:335876/298‑1 (MQ=255)                                                                    gAATGCTGGATCTGCCGGTGGCAAACTTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGTGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTCTGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCa                                                                                                                                                                                                                            >  2:331095/1‑288 (MQ=255)                                                                             aTCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGTTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtggtgg                                                                                                                                                                                                       <  1:622258/300‑1 (MQ=255)                                                                             aTCTGCCGGTGGCAAAATTCATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCtggtgg                                                                                                                                                                                                       <  1:622259/300‑1 (MQ=255)                                                                             aTCTGCCGGTGGCAAAACCCATTACGCCGAATATTACCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGTGTGGTGGCGCtggtgg                                                                                                                                                                                                       <  1:222148/300‑1 (MQ=255)                                                                                            aaTTCATTACGCCGAACATGATCGGCTGCCTGCTGTGGCCACCGTTCTACTTCCTGCCTGGGATCCTGGCGGGCTCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCGGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCAcc                                                                                                                                                                                        <  2:330400/300‑1 (MQ=255)                                                                                                          aaTATTATCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGgtg                                                                                                                                                                          <  1:53361/300‑1 (MQ=255)                                                                                                             attatCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGtat                                                                                                                                                                       >  2:585869/1‑300 (MQ=255)                                                                                                               tatCGGCTGCCTGCTTTGGCCACCTTTTTACTCCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATCCCAGCCGGAATACAGAGCGTCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGTTTGCTGGCTGTGCTGGCGGTTATGGCGCAGCAGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCATTTGTTTTGGCTAACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGtatac                                                                                                                                                                     <  1:169912/300‑2 (MQ=255)                                                                                                               tatCGGCTGCCTGCTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTatat                                                                                                                                                                     <  1:435382/300‑1 (MQ=255)                                                                                                                      tgcctgcTGTGGCCACCGTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATAtt                                                                                                                                                              >  1:647708/1‑300 (MQ=255)                                                                                                                           gctgTGGCCACCGTTTNACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCNCCACCCGTTGATGCCGGTNTATATCGATATTTTGc                                                                                                                                                          >  1:549075/1‑299 (MQ=255)                                                                                                                                 gccaccgTTTTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGt                                                                                                                                                   <  1:171988/296‑1 (MQ=255)                                                                                                                                 gccaccgTTCTACTTCCTGCCTGGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGt                                                                                                                                                   <  1:172165/296‑1 (MQ=255)                                                                                                                                               tCCTGCCTTGGATTCTGGCGGGCGCGGCGATCGATATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATNATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAgg                                                                                                                                     <  2:478422/300‑1 (MQ=255)                                                                                                                                                        ggATTCTGGCGTGCTCAGCGATCGATACTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGTCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATTGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCTCGGCCGTTTGTGTTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTTGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCt                                                                                                                            <  2:239066/300‑1 (MQ=255)                                                                                                                                                              ttgcgggcgCTGCGATCGATATTCCATCCGGAATGCAGAGCGGCGAGTTTAAATGTTTGCTGCTTGCAACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAACAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCAATATTTTGCGTAAAGTGGTTGGGGTTTATGGGATTGTCTTGTGc                                                                                                                       <  2:355726/297‑1 (MQ=255)                                                                                                                                                                gcgggcgCGTCGATCGATATTCCATCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGTTGGCTGGCTGTGCTTGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCggg                                                                                                                    <  2:308483/300‑1 (MQ=255)                                                                                                                                                                              cgatATTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGAc                                                                                                      >  1:350564/1‑300 (MQ=255)                                                                                                                                                                                atatTCCAGCCGGAATGCAGAGCGGCGAGTTTAAATGGTTGCTGCTTTCGACGGCGGTATTTGTGTGGGTTGGTTGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCAATCGTTTAAGTCATTATTTGTCGCTCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGTCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGc                                                                                                    <  2:421565/300‑1 (MQ=255)                                                                                                                                                                                               tGCAGAGCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGTTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATc                                                                                     <  1:625493/300‑1 (MQ=255)                                                                                                                                                                                                  agagCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAgg                                                                                  <  1:453979/300‑1 (MQ=255)                                                                                                                                                                                                  agagCGGCGAGTTTAAATGGTTGCTGCTGGCGACGGCGGTATTTGTGTGGGGTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAATTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAgg                                                                                  <  1:453972/300‑1 (MQ=255)                                                                                                                                                                                                    agCGGCGAGTTTAAATGGTTTCTGCTGGCGACGGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTTGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGcc                                                                                <  2:392943/300‑1 (MQ=255)                                                                                                                                                                                                          gAGTTTAAATGGTTGCTGCTGTCGACGGCTGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGNGGCGCTGGTGGTGTTAATTCGCCACCCGTTGANGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACaa                                                                           <  2:486132/299‑1 (MQ=255)                                                                                                                                                                                                                          tgctgGCGACTGCGGTATTTGTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAgg                                                          <  1:648476/300‑1 (MQ=255)                                                                                                                                                                                                                                        gtatttgtgtgGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGc                                                                                                         >  1:347188/8‑239 (MQ=255)                                                                                                                                                                                                                                        gtatttgtgtgGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGc                                                                                                         <  2:347092/232‑1 (MQ=255)                                                                                                                                                                                                                                        gtatttgtgtgGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAAACGATCGTTTAGGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGc                                                                                                         >  1:347092/8‑239 (MQ=255)                                                                                                                                                                                                                                        gtatttgtgtgGGTTGGTGGCTGGCTGTGCTGGCGGTCATGGCGCAGCGTTAAAGCAACCAATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTGCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGTGGATTGTCTTGTGCGGGTTGCCTGATGc                                                                                                         <  2:347188/232‑1 (MQ=255)                                                                                                                                                                                                                                          atttgtgtgGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGgtgt                                                                                                                                                                         <  2:127772/168‑1 (MQ=255)                                                                                                                                                                                                                                          atttgtgtgGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGgtgt                                                                                                                                                                         >  1:127772/6‑173 (MQ=255)                                                                                                                                                                                                                                              gtgtgGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTTACGCCGTTGATTTCTTCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCAcgcc                                      >  1:526013/2‑300 (MQ=255)                                                                                                                                                                                                                                              gtgtgGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCAcgcc                                      <  1:267271/299‑1 (MQ=255)                                                                                                                                                                                                                                                    gTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATct                                >  1:517141/1‑299 (MQ=255)                                                                                                                                                                                                                                                                 tgtgCTGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACt                   <  1:428726/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      tGGCGGTTATGGCGCAGCGGTAAAGCAACCGATCGTTTAATTCATTATTTGTCGCGCTGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGGATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAAcc               <  1:174835/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                     aGCGGTAAAGCAACCGATCGTTTAAGTCATTATTTGTCGCGCGGCCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTTGGGTTTAGGGGATTGTCTTGTGCGGGTTGCCTGATGCGACGCTGACCGCGTCTTATCAGGCCTACAAAACGCACTGTCCGTAGGTCGGATAAGGTGTTCACGCCGCATCTGACAGTGCATACTAACCCGTAATCCCCAAt  <  1:336534/297‑1 (MQ=255)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                        AGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGCCCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTTATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATTCTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTGCTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGCAGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTGACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTGATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAGGAGATAGTCTTGTGCGGGTTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAACGCACTACCCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAGTGCATACTAACCCGTAATCCCCAAT  >  NC_000913/71675‑72247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑