Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	75,752	2 bp→G	coding (1547‑1548/1656 nt)	sgrR ←	DNA‑binding transcriptional dual regulator SgrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	75,752	0	C	.	96.1%	218.7 / 1.9	51	coding (1548/1656 nt)	sgrR	DNA‑binding transcriptional dual regulator SgrR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base . (21/28);  minor base G (1/1);  total (22/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01
*	NC_000913	75,753	0	A	G	98.1%	180.6 / ‑4.1	52	L516S (TTG→TCG)	sgrR	DNA‑binding transcriptional dual regulator SgrR
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (22/29);  total (23/29)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

TAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAA‑TGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAGTCAATGGGAATGCAATGTTGTAGCAGTGGCACTTCGCATAAATGTGCGAAAACAGAGAAGTCCAGCGGCAGGGTAAAGTTGGCGCTGTTTAGCCAGATATCACTTTCGATCTCTCCTGTATGCCACTGATCGTAGTCGATCTCTTTGATTTTCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGC  >  NC_000913/75475‑76034                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                            tAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATNAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGc                                                                                                                                                                                                                                                                      <  1:142962/300‑1 (MQ=255)     acTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGTGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCAttgctttgct                                                                                                                                                                                                                                                                     >  1:657659/1‑297 (MQ=255)                 aaaaaaGAGTGGCAAAGGACTTTAGAAGGAGCCTCAAACCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTNATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCt                                                                                                                                                                                                                                                       <  2:223362/299‑1 (MQ=255)                                 ggACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCCCGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCCTACTGCGTTGCCCCTGAATGATCCGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCa                                                                                                                                                                                                                                        >  1:465506/1‑298 (MQ=255)                                              aGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTGGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCAt                                                                                                                                                                                                                         <  2:398806/300‑1 (MQ=255)                                                 cTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGAACAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGACATTgc                                                                                                                                                                                                                      >  1:483808/1‑300 (MQ=255)                                                                 ggCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTc                                                                                                                                                                                                      <  1:470371/300‑1 (MQ=255)                                                                       aTCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCCTGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGTGGCACCATTGCTTTGCTGGCTAACAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCAATTGCGCCAGCGGGCGGCGTCGGCtt                                                                                                                                                                                                 >  1:153254/1‑299 (MQ=255)                                                                           ggATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGcc                                                                                                                                                                                              <  2:479588/298‑1 (MQ=255)                                                                            gATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCTTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTTTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGcc                                                                                                                                                                                              <  2:112033/297‑1 (MQ=255)                                                                                         gggTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAAtac                                                                                                                                                                               <  1:520781/299‑3 (MQ=255)                                                                                           gTATTTTCTCAGCGCACGCGTACGCGTTGCACCCCGTTGAGAACGGCGTTGGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAAtacagt                                                                                                                                                                            <  2:182010/300‑6 (MQ=255)                                                                                           gTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAAtacagt                                                                                                                                                                            <  1:8534/300‑6 (MQ=255)                                                                                             aTTTTCTCATCGCACGCGTACGCGTGGCACCCCGTTGAGAACGTCGTTAGTGTAGGGATTTTGTTATCAACCAGCAATCATGGATCCGTTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTTGCGACCAGTTGCTTGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAAtacagt                                                                                                                                                                            <  1:8623/298‑6 (MQ=255)                                                                                                   tcAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGtgttggag                                                                                                                                                                     <  2:657659/299‑4 (MQ=255)                                                                                                     aGCGCACGCGTACGCTTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCTAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGc                                                                                                                                                                    <  2:55152/298‑1 (MQ=255)                                                                                                      gcgcACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGACATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAgc                                                                                                                                                                 >  1:528615/1‑299 (MQ=255)                                                                                                            gcgtacgcgtTGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCNGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGNGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCAc                                                                                                                                                            <  2:511474/299‑1 (MQ=255)                                                                                                                     tGGCACCCCGTTGAGAACTGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCacaag                                                                                                                                                  <  2:154753/300‑5 (MQ=255)                                                                                                                     tGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCacaag                                                                                                                                                  <  1:365995/300‑5 (MQ=255)                                                                                                                            ccGTTGAGAACGGCGTTAGTGTAGTGATTTTTTTATCCACCAGCAATCCTGGACCCGGTGGCGCCAACCACACTGATTTAAAATCGAACCCGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGAACAGTTTCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCACCAGCGGGCGGCATCGGCTTGCCAGTCGATCGGAATCCAGTGTTGGAGCAACGGCACCTCGCACAAAtgcgc                                                                                                                                             >  1:566683/1‑295 (MQ=255)                                                                                                                            ccGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGNTCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGtgttggag                                                                                                                                                                     <  1:492759/274‑4 (MQ=255)                                                                                                                            ccGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCCTACTGCGTTGCCCCTNAATGATCCGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTTATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTcgatctgaatacagtgttggag                                                                                                                                                                     >  2:492759/1‑253 (MQ=255)                                                                                                                                        gCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGAACAGTTGCTGGCACCAGTTCGCCAGGTTCCTTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGaacagcgaaaaa                                                                                                                                >  1:344675/1‑289 (MQ=255)                                                                                                                                        gCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCNGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGaacagcgaaaaat                                                                                                                               >  2:505444/1‑289 (MQ=255)                                                                                                                                        gCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAAGCCGCGCATACTGCGTTGCCCCTGAATGATCCGCAAGTGGTGGAGCAG‑CGGCACCATTGCTTTGCTGGCGACCAGCTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTTGGCTTGCCAATCAATCGGAATACAGTGTTGGCGCAGCGGCACCTCGCACAAGTGCGCGaacagcgaaaaat                                                                                                                               >  2:100024/1‑289 (MQ=255)                                                                                                                                          gTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGaacagcgaaaaatcc                                                                                                                             <  1:618945/300‑14 (MQ=255)                                                                                                                                              gtgtagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCCAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTAGCCATTGCGCCAGCGGGCGGCGTCGGCTTTCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGaacagcgaaaaatcca                                                                                                                            >  2:345197/1‑283 (MQ=255)                                                                                                                                                          tGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCGTTTCGCCATTGCGCCAGCGGGCTGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGt                                                                                                             <  1:372773/300‑1 (MQ=255)                                                                                                                                                          tGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGt                                                                                                             <  1:371970/300‑1 (MQ=255)                                                                                                                                                                  aCCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCCGCGTAAAGTTGGCGCt                                                                                                      >  2:426361/1‑299 (MQ=255)                                                                                                                                                                          tCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCa                                                                                             >  2:236459/1‑300 (MQ=255)                                                                                                                                                                            aTGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAg                                                                                            <  1:154985/299‑1 (MQ=255)                                                                                                                                                                              ggATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTAGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGata                                                                                         >  2:558934/1‑300 (MQ=255)                                                                                                                                                                                        gcgcAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCATGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTAGACATTGCGCCAGCGGGCGGCGTCGGCTTGCAAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGa                                                                               >  2:244700/1‑300 (MQ=255)                                                                                                                                                                                                   gcggATTTAAAATCGAACCAGCCGAGGGGATTTATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTTGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCttc                                                                     <  2:48310/296‑3 (MQ=255)                                                                                                                                                                                                      gATTTAAAATCGAACCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCttcatgc                                                                 >  1:406678/1‑294 (MQ=255)                                                                                                                                                                                                        tttAAAATCGAACCAGCCGAGGGTATTCATGCTTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCATTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCttcat                                                                   <  2:191418/296‑5 (MQ=255)                                                                                                                                                                                                                   aCCAGCCGAGGGTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTTGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAgc                                                    <  1:195096/300‑2 (MQ=255)                                                                                                                                                                                                                          taGGGTATTCATGCGTATGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAgctgatc                                               <  2:308337/297‑7 (MQ=255)                                                                                                                                                                                                                            gggTATTCATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCtt                                           >  1:448710/1‑300 (MQ=255)                                                                                                                                                                                                                                   cATGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGAtttc                                    >  1:631673/1‑299 (MQ=255)                                                                                                                                                                                                                                     tGCGTAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGAtttcca                                  >  1:242519/1‑297 (MQ=255)                                                                                                                                                                                                                                              cgcgCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGAGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGGCACTGATCGTAGCTGATCTATTTGATTTCCAGCGTGACCt                         >  2:73867/1‑300 (MQ=255)                                                                                                                                                                                                                                                   aTACTGCGTTGCCCCTGAATGATCAGCTAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGAcc                          <  2:464154/294‑1 (MQ=255)                                                                                                                                                                                                                                                   aTACTGCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGa                    <  1:524401/300‑1 (MQ=255)                                                                                                                                                                                                                                                   aTACTGCGTTGCCCCTGAATGATCAGCCAGTGG‑GGGATAAGCGGCACCATTGCGTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGGGACCTgggga                    <  1:524394/300‑4 (MQ=255)                                                                                                                                                                                                                                                    tACTGCGTTGTCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCNATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGAc                   <  2:226250/300‑1 (MQ=255)                                                                                                                                                                                                                                                        gCGTTGCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGc               >  1:328845/1‑300 (MQ=255)                                                                                                                                                                                                                                                             gCCCCTGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGACATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTATCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCAAGaa          >  2:352599/1‑300 (MQ=255)                                                                                                                                                                                                                                                                  tGAATGATCAGCCAGTGGTG‑GATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATTTCCAGCGTGACCTGGTGACTTGCCAGAAt         <  1:112596/296‑1 (MQ=255)                                                                                                                                                                                                                                                                     aTGATCAGCCAGTGGT‑NGATAAGCGGCACCATTGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGGTTCATTTCGCCATTGCGCCAGCGGGCGGCGTCGGCTTGCCAGTCGATCGGAATACAGTGTTGGAGCAGCGGCACCTCGCACAAGTGCGCGAACAGCGAAAAATCCAGCGGCAGCGTAAAGTTGGCGCTGTTAAGCCAGATATCGCTCTCGATCTCTCCTTCATGCCACTGATCGTAGCTGATCTCTTTGATNTCCAGCGTGACCTGGTGANTTGCCAGAATCtgctgc  >  1:583103/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                            TAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCGCCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCGTTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAA‑TGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAGTCAATGGGAATGCAATGTTGTAGCAGTGGCACTTCGCATAAATGTGCGAAAACAGAGAAGTCCAGCGGCAGGGTAAAGTTGGCGCTGTTTAGCCAGATATCACTTTCGATCTCTCCTGTATGCCACTGATCGTAGTCGATCTCTTTGATTTTCAGCGTGACCTGGTGACTTGCCAGAATCTGCTGC  >  NC_000913/75475‑76034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑