Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	66,518	T→C	E11E (GAA→GAG)	araD ←	L‑ribulose‑5‑phosphate 4‑epimerase AraD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	66,518	0	T	C	100.0%	186.2 / NA	56	E11E (GAA→GAG)	araD	L‑ribulose‑5‑phosphate 4‑epimerase AraD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/35);  total (21/35)

GCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAA  >  NC_000913/66231‑66789                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                gCCTGCGCCCAGATAGTGGCGTGGCGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCt                                                                                                                                                                                                                                                                       <  1:39815/298‑1 (MQ=255)           aGATAGTGGCGTGGCGCGGGTGTGTGTGCACAATGCCGCCAATGGAAGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCCCCGGTTTCGATGTTAAACACGAACATATCGTCAGCGGTCCTGATGCTGTAATCGACGACAGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGTCCCATGTGAGCGTGGCCCGGTTATTTTTTGGCAGCGCCAGGTTGGCCCCTAACCACTGGCGTTTgaggt                                                                                                                                                                                                                                                           >  2:78963/1‑298 (MQ=255)            gatagtggcgtggcGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACATGGCGTTTgaggtc                                                                                                                                                                                                                                                          >  1:604153/5‑297 (MQ=255)            gatagtggcgtggcGCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCAACGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGTGTTTggggtc                                                                                                                                                                                                                                                          >  2:472913/5‑295 (MQ=255)                     gtggcgCGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACa                                                                                                                                                                                                                                                  <  1:633152/299‑1 (MQ=255)                        gcgcGAGTGTGTGTGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCAACGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCACCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGtt                                                                                                                                                                                                                                              >  2:346520/1‑300 (MQ=255)                                gtgtgtGCACAATGCCNCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATNCCTGGCGTTTGAGATCTTNTAACATGTTGACTCCtt                                                                                                                                                                                                                                      >  1:580499/6‑300 (MQ=255)                                  gtgtGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCg                                                                                                                                                                                                                                    <  2:690594/297‑1 (MQ=255)                                    gtGCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGt                                                                                                                                                                                                                                   >  1:605854/2‑299 (MQ=255)                                      gCACAATGCCGCCAATGGACGGGAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCAGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCACCATGTGAGCGTGAACAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTATAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTAGTGcc                                                                                                                                                                                                                                >  2:128290/1‑300 (MQ=255)                                                        acgggAATGCCTGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATc                                                                                                                                                                                                              <  1:630465/298‑1 (MQ=255)                                                                   tGATAGAGCAGTCGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACaaa                                                                                                                                                                                                   <  1:212577/300‑1 (MQ=255)                                                                            agtcGGTGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCg                                                                                                                                                                                          <  1:178388/297‑1 (MQ=255)                                                                                  tGAGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAgg                                                                                                                                                                                    <  1:266479/300‑1 (MQ=255)                                                                                    agtttgcgtatcgtagtagtgCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGcc                                                                                                                                                                                  <  2:364693/280‑1 (MQ=255)                                                                                    agtggacatatcggaggaagacctcctcgcaCCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGGCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGNGAGCGTGACCAGGTTATGTTTTGGCATCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGcc                                                                                                                                                                                  <  2:496318/270‑1 (MQ=255)                                                                                    aGTTGGCGTATCGTAGGAGTGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGTTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCGCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGTCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGcc                                                                                                                                                                                  <  2:334010/300‑1 (MQ=255)                                                                                    aGTTGGCGTATCGGATGGGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTCGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGcc                                                                                                                                                                                  <  2:43154/300‑1 (MQ=255)                                                                                    aGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGTAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGcc                                                                                                                                                                                  <  2:566469/300‑1 (MQ=255)                                                                                    aGTTGGCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGcc                                                                                                                                                                                  <  1:350583/300‑1 (MQ=255)                                                                                         gcgtatcgtagtaaggCTTTTTCGCACCTTCAACCACGTCACCGGTTTAGATGCTAACCACGACCATATCGTTAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGNGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCCAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCCTATCCGGCCTACGAAATCGCAGCGTGTAGGcc                                                                                                                                                                                  <  2:496237/281‑1 (MQ=255)                                                                                         gCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATNAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCAAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATa                                                                                                                                                                             >  2:510231/1‑300 (MQ=255)                                                                                         gCGTATCGGAGGAGGGCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATa                                                                                                                                                                             <  1:166366/300‑1 (MQ=255)                                                                                                      gggCTTTTTCGCACCTNCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCAGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACANAATCGCAGCGTGTAGGCCNGATAAGACGCGCCAGc                                                                                                                                                                 >  1:573420/1‑299 (MQ=255)                                                                                                        gCTTTTTCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACTACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGt                                                                                                                                                               <  2:154583/299‑1 (MQ=255)                                                                                                            tttCGCACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTAAGCGTTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCAt                                                                                                                                                          <  1:394204/300‑1 (MQ=255)                                                                                                                  accgtCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCCCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGc                                                                                                                                                     <  2:469366/295‑1 (MQ=255)                                                                                                                  aCCTTCAACCATTTCACTGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGc                                                                                                                                                     <  2:106572/299‑1 (MQ=255)                                                                                                                   ccTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGcc                                                                                                                                                                                  <  1:624580/269‑1 (MQ=255)                                                                                                                   ccTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAAAATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGACTACAAAATCGCAGCGTGTAGGcc                                                                                                                                                                                  >  2:624580/1‑269 (MQ=255)                                                                                                                                             gCTAACCACGACCATATCGTCAGCGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATc                                                                                                                         <  1:637484/300‑1 (MQ=255)                                                                                                                                                       aCCATATCGTCAGTGGTCATGATGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATTAACGGCGCTGAAGTTGCCCCATGTGAGCGTGAATAGGCTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGGTCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCAAGCGTAGCATCAGGCGTTGAATACAGGATGCGCTTTGTTTATCCAGCCGACaa                                                                                                               >  2:623639/1‑300 (MQ=37)                                                                                                                                                                         atgatgCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACTGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTTAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGcc                                                                                             <  1:93103/300‑1 (MQ=255)                                                                                                                                                                         atgatgCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGcc                                                                                             <  2:551293/300‑1 (MQ=255)                                                                                                                                                                         atgatgCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGcc                                                                                             <  1:42119/300‑1 (MQ=255)                                                                                                                                                                                 gTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGTGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTTGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGGATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGcc                                                                                             <  2:649533/292‑1 (MQ=255)                                                                                                                                                                                                         cacaAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCAATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATTTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCTGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCAGGCCTACAAAATCGGAAAGCGTAGGCATGATAAGACGCGCCAGCGTCGCATCAGGCGtt                                                             >  2:193875/1‑300 (MQ=25)                                                                                                                                                                                                           caAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGa                                                           >  1:332912/1‑300 (MQ=255)                                                                                                                                                                                                                   ccgcGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGGCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAACATCGGAACGCATAAGCCTGATAAGAAGCGCAAGCGTCGCATCAGGCGTCGACTGCCGGa                                                   >  2:188412/1‑300 (MQ=25)                                                                                                                                                                                                                   ccgcGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAACGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGTCTCTAATACCTGGCGCTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGATTTGCTTATCCGGCATACAAAATCGAAGCGTGTAAGCCTGATAAGAAGCGCAAGCGTCGCATCAGGCGCTGAATGCCGGATgcgc                                                                                                                                   >  2:186473/1‑220 (MQ=255)                                                                                                                                                                                                                           gcgATCAACTGCGCTGACGTTGCCCCATGGGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTTGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCtt                                            <  1:425161/299‑1 (MQ=255)                                                                                                                                                                                                                                  aCGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATc                                    <  1:381387/300‑1 (MQ=255)                                                                                                                                                                                                                                  aCGGCGCTGACGTTGCCCCATGGGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATc                                    <  1:380360/300‑1 (MQ=255)                                                                                                                                                                                                                                     gcgcTGACGTTGCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATACCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGAAGCGCCAGTGTCGCATCCGGCGTTAAACGCCGGATGTGCTTTGCTTATcc                                   >  2:156657/1‑298 (MQ=25)                                                                                                                                                                                                                                                gCCCCATGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATc                      >  1:647727/1‑300 (MQ=255)                                                                                                                                                                                                                                                     aTGTGAGCGTGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGc                 >  1:263539/1‑300 (MQ=255)                                                                                                                                                                                                                                                              tGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTTAGATCTTCGAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGcc        <  1:278939/300‑1 (MQ=255)                                                                                                                                                                                                                                                              tGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTNGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGcc        <  1:144221/300‑1 (MQ=255)                                                                                                                                                                                                                                                              tGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACCAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCAGCCTAACAAATCGGAACGCGTAGGCCTGATAAGACGCGACAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTa            >  2:296560/1‑296 (MQ=25)                                                                                                                                                                                                                                                              tGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGcc        >  1:14108/1‑300 (MQ=255)                                                                                                                                                                                                                                                              tGACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCAGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGcc        <  1:278624/300‑1 (MQ=255)                                                                                                                                                                                                                                                               gACCAGGTTATGTTTTTGCAGCGCCAGGTTTGCCTCGAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAGTCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATTAGGCGTTGAATGCCGGAGGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTTTAGGcc        <  2:141370/299‑1 (MQ=37)                                                                                                                                                                                                                                                               gACCAGGTTATGTTTTGGCGGCGCCAGGTTGGCCTCGAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGcc        <  2:675803/299‑1 (MQ=255)                                                                                                                                                                                                                                                               gACCAGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGAGGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGcc        <  2:580499/299‑1 (MQ=255)                                                                                                                                                                                                                                                                   aGGTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATTTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATa   <  1:346520/300‑1 (MQ=255)                                                                                                                                                                                                                                                                    ggTTATGTTTTGGCAGCGCCAGGTTGGCCTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGGAACGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATaa  >  1:162330/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                GCCTGCGCCCAGATGGTGGCGTGGCGCGAGTGCGTATGCACAATGCCGCCAATGGAGGGGAATGCCTGATAGAGCAGCCGGTGAGTTGGCGTGTCGGAGGAGGGCTTTTTCGTACCTTCAACCACTTCACCGGTTTCGATGCTAACCACGACCATATCGTCAGCGGTCATGACGCTGTAATCGACGCCGGAAGGTTTGATCACAAAGACGCCGCGCTCGCGATCAACGGCGCTGACGTTGCCCCATGTGAGCGTGACCAGGTTGTGTTTTGGCAGCGCCAGGTTGGCTTCTAATACCTGGCGTTTGAGATCTTCTAACATGTTGACTCCTTCGTGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGCGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCGTTGAATGCCGGATGCGCTTTGCTTATCCGGCCTACAAAATCGCAGCGTGTAGGCCAGATAA  >  NC_000913/66231‑66789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑