Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,223,646	C→T	G338G (GGC→GGT)	ebgA →	evolved beta‑D‑galactosidase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,223,646	0	C	T	100.0%	227.4 / NA	64	G338G (GGC→GGT)	ebgA	evolved beta‑D‑galactosidase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (38/26);  total (38/26)

TGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAA  >  NC_000913/3223358‑3223939                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                      tGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAAcc                                                                                                                                                                                                                                                                                                <  1:100459/296‑1 (MQ=255) tGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCg                                                                                                                                                                                                                                                                                               <  1:610944/297‑1 (MQ=255)              cTGGAATATACCCTGTTTGATGGCGAACGCGTAGTGCACAGCAGCGCCACTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAGCAGCCGCAGCAATGGTCAGCAGAATTCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGTGCGACGGTCTGTTCTGGATCAATAACCATTATATGATGCCGCATGGTGTAAACCATCAAGAAAACGaccat                                                                                                                                                                                                                                                                               >  2:102989/1‑300 (MQ=255)                  aaTATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAANTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCa                                                                                                                                                                                                                                                                           >  1:499132/1‑300 (MQ=255)                  aaTATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCa                                                                                                                                                                                                                                                                           >  1:49671/1‑300 (MQ=255)                   atatACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACANCCGCAGCAATGGTCAGCAGAATCCCCTTATCTNTACCATCTGGTCATGAAGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAAAGATCATCGCaa                                                                                                                                                                                                                                                                          >  2:526320/1‑300 (MQ=255)                         gcgagatgtgtataagggacaggtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATTTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGAAAAGGcc                                                                                                                                                                                                                                                                     >  1:386641/22‑299 (MQ=255)                                    gCGAACGCGTGGTGCACAGCAGCGCCGTGGATCATTGGGCAATTGAAAAACTTACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCATCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCTTTGGCTTCCTTGATATCAAGGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATTTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGaa                                                                                                                                                                                                                                                         <  2:285683/300‑1 (MQ=255)                                      gAACGCTTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGANGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATg                                                                                                                                                                                                                                                       <  2:217005/300‑1 (MQ=255)                                      gAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGaa                                                                                                                                                                                                                                                         <  2:286046/298‑1 (MQ=255)                                      gAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATg                                                                                                                                                                                                                                                       <  2:141965/300‑1 (MQ=255)                                               gtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTc                                                                                                                                                                                                                                              >  1:23639/1‑300 (MQ=255)                                               gtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCAGTTGGAATGGATCGCGTc                                                                                                                                                                                                                                              >  1:324377/1‑300 (MQ=255)                                               gtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTGATCTTTAACATCTGGTCATGACGCTGAAAGACCCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTc                                                                                                                                                                                                                                              >  2:606535/1‑300 (MQ=255)                                               gtgCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCATTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAAACGTCACGAAAAAGATCATCGCAAAGGCCGCGCCGTGGGAATgggtc                                                                                                                                                                                                                                                   >  2:293386/1‑292 (MQ=255)                                                            ccATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAtctac                                                                                                                                                                                                                                 >  2:430813/1‑298 (MQ=255)                                                                      tttGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCNTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGaa                                                                                                                                                                                                                       <  1:508900/300‑1 (MQ=255)                                                                             aTTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGcac                                                                                                                                                                                                                <  2:446227/300‑1 (MQ=255)                                                                                  aaaaCTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAatat                                                                                                                                                                                                           <  1:439683/300‑1 (MQ=255)                                                                                  aaaaCTGACCAGCGCCAGCTTTGCTTCTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAatat                                                                                                                                                                                                           <  2:98847/300‑1 (MQ=255)                                                                                             gcgcCAGTTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGc                                                                                                                                                                                                >  2:664181/1‑300 (MQ=255)                                                                                                   gctttgctttTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTAcc                                                                                                                                                                                           >  1:665678/1‑299 (MQ=255)                                                                                                      ttgctttTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTc                                                                                                                                                                                       <  1:595794/300‑1 (MQ=255)                                                                                                           tttACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTAc                                                                                                                                                                                  <  1:192585/300‑1 (MQ=255)                                                                                                                 gTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTACGTGCGTACCGCTCACTACCCGaa                                                                                                                                                                             >  2:4280/1‑299 (MQ=255)                                                                                                                  tCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACg                                                                                                                                                                           <  2:53544/300‑1 (MQ=255)                                                                                                                             cagcaATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGtttt                                                                                                                                                                >  1:14050/1‑300 (MQ=255)                                                                                                                                    ggTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAAc                                                                                                                                                         >  1:189756/1‑300 (MQ=255)                                                                                                                                    ggTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAAc                                                                                                                                                         >  2:681260/1‑300 (MQ=255)                                                                                                                                     gTCAGCAGAATCCCCTTATCTTTACCATCTGTTCATTACGCTGAAAGACGCCAACGGCAACGTTCTGGAATTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATTCGCGTTTTTACGAAc                                                                                                                                                         <  2:357334/299‑1 (MQ=255)                                                                                                                                         gcagAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACtgtgt                                                                                                                                                    >  2:636036/1‑300 (MQ=255)                                                                                                                                              aTCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTTTGatat                                                                                                                                               <  1:398491/300‑1 (MQ=255)                                                                                                                                              aTCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGatat                                                                                                                                               <  1:398478/300‑1 (MQ=255)                                                                                                                                                                  tGGTCAGGACGCTGAAAGACGCCAACGGCAACGTTCTGTAATTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGAt                                                                                                                             <  2:69935/298‑1 (MQ=255)                                                                                                                                                                         gACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTTTGATGGCGGAAAc                                                                                                                    <  1:289440/300‑1 (MQ=255)                                                                                                                                                                          aCGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTACAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCAGAACAATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAAcc                                                                                                                   >  2:191715/1‑300 (MQ=255)                                                                                                                                                                                                aCGTTCTGGAAGTGGTGCCAAAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTACAGTTGATGAAGGAGCACAATATCAACTCCGTGCGTACCGCTCACTAACCGAACGATCCGCGCTTTTACGAACTGTGTGATATCTACGGACTGTTTGTGATGGCAGAAACCGAAGTAGAACCGCACGGCttt                                                                                              >  2:943/1‑299 (MQ=255)                                                                                                                                                                                                   ttcgggAAGTGGTGCCACAACGCGTTGGCTTCCTTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTa                                                                                          <  1:325235/296‑1 (MQ=255)                                                                                                                                                                                                   ttCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTa                                                                                          <  1:324126/300‑1 (MQ=255)                                                                                                                                                                                                      tGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATg                                                                                       <  2:654641/300‑1 (MQ=255)                                                                                                                                                                                                           gtggtgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGc                                                                                  >  1:643776/1‑300 (MQ=255)                                                                                                                                                                                                                 ccACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATAtt                                                                            >  1:346567/1‑300 (MQ=255)                                                                                                                                                                                                                              gCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACcg                                                               <  2:189756/300‑1 (MQ=255)                                                                                                                                                                                                                                       gcgagatgtgtataagagacaggTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGAAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTGATGTCGGCGATATTAGCCGTATTACCGACGATCCGc                                                      >  1:525702/23‑300 (MQ=255)                                                                                                                                                                                                                                       atatCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGc                                                      >  1:685267/1‑300 (MQ=255)                                                                                                                                                                                                                                         atCAACGTGCGCGACGGGCTGTTCTGGATCAATAACCCTTATGTAATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTACTACCGACGATCCGCAg                                                    <  1:233427/300‑1 (MQ=255)                                                                                                                                                                                                                                         atCAACGTGCGCGACGGCCTGTTCTGGATCAATAACCCTTATGTAATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAg                                                    <  1:232899/300‑1 (MQ=255)                                                                                                                                                                                                                                         atCAAAGTGCGCGACGGCCTGTTCTGGATCAATAACCCTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAg                                                    <  1:232889/300‑1 (MQ=255)                                                                                                                                                                                                                                                        ggTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCATAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGAGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCAGTATTACCGACGATCCGCAGTGGGAAAAAGTCTAc                                     >  2:257208/1‑300 (MQ=255)                                                                                                                                                                                                                                                        ggTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTa                                      >  2:395662/1‑299 (MQ=255)                                                                                                                                                                                                                                                        ggTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTAc                                     >  1:483739/1‑300 (MQ=255)                                                                                                                                                                                                                                                         gTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTAc                                     >  1:176010/1‑299 (MQ=255)                                                                                                                                                                                                                                                         gTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCAGTATTACCGACGATCCGCAGTGGGAAAAAGTCTa                                      >  2:290291/1‑298 (MQ=255)                                                                                                                                                                                                                                                             gTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGNGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGCCTACGTCGa                                >  2:543148/1‑300 (MQ=255)                                                                                                                                                                                                                                                             gTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAACAGTCTACGTCGa                                >  2:389685/1‑300 (MQ=255)                                                                                                                                                                                                                                                             gTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGa                                >  1:425079/1‑300 (MQ=255)                                                                                                                                                                                                                                                             gTTCTGGATCAATAACCGTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGAGTACCGCTCACTACCCGAACGATCCGNGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGACTCGCAAGGGGAAAAAGTCTACGTCGa                                >  2:220191/1‑300 (MQ=255)                                                                                                                                                                                                                                                                              gTTATGTGATGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCata               >  1:533886/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      aTGCTGCACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCAcgc        >  1:23805/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                      aTGCTGCACGGTGTCAACCGTCAAGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGAAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCAcgc        >  2:14621/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                           gcACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAANTCCGTGCGTACCGCTCACTACCCGAACGATCCNCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTCCCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGaa  >  2:519406/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           gcACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGt                                   <  1:262457/267‑1 (MQ=255)                                                                                                                                                                                                                                                                                           gcACGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTACGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTAAGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATCTTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGt                                   >  2:262457/1‑267 (MQ=255)                                                                                                                                                                                                                                                                                           gaaCGGTGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGACGCAGCACAATATCAANTACGTGCGTACCGCTCACTAACCGAACGATCCNCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTCGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGCGCGTATTGTTCGCCATATCCACGCGCAGaa  >  2:519405/3‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                      TGTCGTCACGACGCTGGAATATACCCTGTTTGATGGCGAACGCGTGGTGCACAGCAGCGCCATTGATCATTTGGCAATTGAAAAACTGACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAA  >  NC_000913/3223358‑3223939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑